Objective To explore the clinical value of blood urea nitrogen(BUN)combined with carbon dioxide binding power(CO2-CP)in predicting the short-term prognosis in the patients with acute ischemic stroke(AIS)undergoing intravenous thrombolysis.Methods The medical case data of 238 patients with AIS treated by recombinant tissue plasminogen activator(rt-PA)intravenous thrombolysis in the neurology de-partment of the First Affiliated Hospital of Nanyang Medical College from January 2023 to January 2024 were retrospectively analyzed.The general data and laboratory test results were collected.The general data and la-boratory detection results were collected.According to the results of modified Rankin Scale(mRS)on 90 d,the patients were divided into the good prognosis group(mRS＜3 points,157 cases)and poor prognosis group(mRS≥3 points,81 cases).The levels of WBC count,RBC count,PLT,Na+,K+,TC,TG,HDL-C,Fib,D-di-mer,HCY,BUN,Cr,UA,UA/Cr ratio and CO2-CP before intravenous thrombolysis were compared between the two groups.The multivariate logistic regression was used to analyze the risk factors of poor prognosis in the patients with AIS intravenous thrombolysis treatment.The receiver operating characteristic(ROC)curve was drawn to evaluate the predictive value of the corresponding indicators in the short-term prognosis of the AIS patients with intravenous thrombolysis treatment.Results The NIHSS score,Glu,BUN and CO2-CP lev-els at admission had statistically significant difference between the two groups(P＜0.05).The multivariate logistic regression analysis results showed that BUN(OR=1.308,95％CI:1.119-1.528)and CO2-CP(OR=0.676,95％CI:0.589-0.776)were the independently influencing factors for the short-term prognosis in the patients with AIS intravenous thrombolysis treatment.The ROC curve analysis results showed that the area under the curve(AUC)of BUN and CO2-CP combination in the evaluation of short-term poor prognosis in the patients with AIS intravenous thrombolysis treatment was 0.850,the 95％CI was 0.800-0.901,the sensitivity was 79.0％,the specificity was 82.8％,which had good predictive value.Conclusion The combina-tion of BUN and CO2-CP in predicting the short-term prognosis of the patients with AIS intravenous throm-bolysis treatment has the higher clinical value.

In this paper, the history and current situation of environmental health standardization in China are reviewed, and the experience and shortcomings in the process of environmental health standardization in China are analyzed, suggestions for the next step of environmental health standards are also put forward.

In this paper, the history and current situation of environmental health standardization in China are reviewed, and the experience and shortcomings in the process of environmental health standardization in China are analyzed, suggestions for the next step of environmental health standards are also put forward.

OBJECTIVETo establish an in vitro culture model of single human hair follicle, and observe their morphological and histological changes.

METHODSHuman hair follicles were isolated from the volunteer patients. After dissecting follicles into single, follicles in growth phase were cultured in Williams E without any serum. This experiment included 3 groups: single follicle without sebaceous gland and other surrounding tissue (control group); single follicle with sebaceous gland and without the other surrounding tissue( experiment group A); single follicles with sebaceous gland and the other surrounding tissue (experiment group B). The survival rate, survival time, growth rate, multiplication capacity and apoptosis of cultured follicles and their morphological and histological changes were observed sequentially.

RESULTSThe hair follicles in experiment groups showed a better viability and a higher growth rate than those in control group. And the follicles in group B could keep growing for more than 25 days, which was longer than those in group A. Moreover, the sebaceous gland and the other surrounding tissue in group B showed great induction effect on follicle-cell proliferation and anti-apoptosis.

CONCLUSIONThe in vitro culture model of signal human hair follicles (single follicles including epidermis, sebaceous gland and the other surrounding tissue) had optimized internal environment which is similar to in vivo internal environment.

Culture Techniques ; Hair Follicle ; anatomy & histology ; cytology ; growth & development ; Humans ; In Vitro Techniques ; Sebaceous Glands ; Time Factors

Objective To investigate the potential clinical prognostic factors of severe traumatic brain injury.Methods Two hundred and thirteen severe traumatic brain injury patients,admitted to our hospital from May 2013 to July 2015,were chosen in our study.Their clinical data were retrospectively analyzed;prognostic factors,including age,gender,mGCS scores (movement GCS scores),improved CT scores,brain hernia,volume of intracranial hematoma,range of cerebral contusion and laceration,and location of intracranial hematoma,were estimated using Chi square test,and after the Chi square test,the factors enjoying statistical significance were analyzed by COX proportional hazards regression model.Results The mortality rate was 15.9％ (34/213).Death occurred within 30 days of admission,and death mostly occurred within 10 days of admission (1-10 d:23 patients;11-20 d:9 patients;20-30 d:2 patients).Cox proportional hazards regression analysis indicated that age,GCS scores (movement GCS scores),improved CT scores,and brain hernia were the prognostic risk factors in patients with severe traumatic brain injury,while gender,volume of intracranial hematoma,range of cerebral contusion and laceration,and location of intracranial hematoma were not.Conclusion Age,mGCS scores (movement GCS scores),improved CT scores,and brain hernia dare important factors affecting the prognosis of patients with severe traumatic brain injury.