Objective To understand the influencing factors of chronic dyslipidemia in T2DM patients who signed a contract for diabetes point system management in Qingpu District, and to provide a basis for comprehensive intervention and prevention and control of dyslipidemia in T2DM patients and to optimize the management strategy of Qingpu District diabetes point system. Methods Among the T2DM patients who signed the diabetes point system from 2017 to 2023, patients with chronic dyslipidemia and normal blood lipids were selected and included in the case group and the control group, respectively. A case-control study was conducted with 1:1 matching by age and gender to analyze the factors influencing dyslipidemia. Results Multifactorial paired logistic regression analysis showed that overweight/obesity and central obesity and smoking in T2DM patients increased the risk of dyslipidemia by 1.93, 2.27, and 2.16 times, respectively. Long-term use of lipid-lowering drugs, duration of diabetes for 5 years or more, regular physical exercise, knowledge of blood lipid status, and married status could reduce the risk of dyslipidemia in T2DM patients (OR values were 0.547, 0.452, 0.685, 0.386 and 0.354, respectively). Current complications (history of stroke, coronary heart disease, and renal insufficiency) were also associated with dyslipidemia (OR=1.802, 95% CI:1.125-2.888). Conclusion The management of diabetes point system in Qingpu District should strengthen the feedback and interpretation of blood lipid monitoring results, improve patients’ health awareness of blood lipid management, and actively take comprehensive management of lifestyle intervention and drug treatment to effectively control blood lipid and reduce the occurrence of related complications.

Objective:To select and optimize the conditions of urine cobalt determination by graphite furnace atomic absorption spectroscopy and establish a method for urine cobalt determination.Methods:In April 2020, the matrix modifier and spectrum wavelength were selected by mathematical statistics method, the heating procedure of graphite furnace was optimized, the variance of orthogonal test results was analyzed, and the working curve was quantified by external standard method.Results:The results showed that the diammonium hydrogen phosphate was a matrix modifier with a wavelength of 240.7 nm, and the optimized graphite furnace heating procedure showed a good linear relationship at 5-80 μg/L. The correlation coefficient was 0.9991. The detection limit was 0.9 μg/L, the lowest detection concentration was 1.8 μg/L. The recovery was 94.0%-101.4%, and the precision was 2.6%-5.8%.Conclusion:The orthogonal design method can efficiently and scientifically optimize the optimal combination of the various factor levels of graphite furnace atomic absorption spectrometry. The determination method established based on this has high precision and good accuracy, and can be applied to the determination of cobalt in urine.

Objective:To select and optimize the conditions of urine cobalt determination by graphite furnace atomic absorption spectroscopy and establish a method for urine cobalt determination.Methods:In April 2020, the matrix modifier and spectrum wavelength were selected by mathematical statistics method, the heating procedure of graphite furnace was optimized, the variance of orthogonal test results was analyzed, and the working curve was quantified by external standard method.Results:The results showed that the diammonium hydrogen phosphate was a matrix modifier with a wavelength of 240.7 nm, and the optimized graphite furnace heating procedure showed a good linear relationship at 5-80 μg/L. The correlation coefficient was 0.9991. The detection limit was 0.9 μg/L, the lowest detection concentration was 1.8 μg/L. The recovery was 94.0%-101.4%, and the precision was 2.6%-5.8%.Conclusion:The orthogonal design method can efficiently and scientifically optimize the optimal combination of the various factor levels of graphite furnace atomic absorption spectrometry. The determination method established based on this has high precision and good accuracy, and can be applied to the determination of cobalt in urine.

Objective: To establish an HPLC method for determining p-Anisidine in workplace air. Methods: An C₁₈ capillary column was used with the detection wavelength of 240 nm, the methanol solution (v/v=62:38) as mobile phase, the flow rate was 0.9 ml/min, and the injection volume was 20 μl. Results: The linear range (μg/ml) for p-Anisidine was 0~100; correlation coefficient was 0.999 7; the detection limit was 0.15 μg/ml; the average precision of RSD was 4.4%. The sampling efficiency was more than 95% using silicone tube as sampling filter. Sample can be stored at room temperature for more than 7 d. Conclusion the method appered to be accurate, simple, sensitive and reliable, also have a high sampling efficiency, and can be usd to determine p-anisidine in workplace air simultaneously.

Objective To investigate the correlation of occupational stress with the serum levels of immunoglobulins(IgG,IgM,and IgA) and complement(C3 and C4).Methods In May 2011,convenience sampling and cluster sampling were used to select 225 policemen from a local police station as study subjects.A questionnaire was used to investigate demographic features and occupational stress,and the immunoturbidimetric assay was applied to measure the serum levels of IgG,IgM,IgA,and complement C3 and C4.Results Positive affectivity was positively correlated with the concentration of IgG(r=0.084,P＜0.01);positive affectivity,coping strategy,and sleep quality were positively correlated with the concentration of IgM (r=0.146,0.155,and 0.203,all P＜0.05);negative affectivity was negatively correlated with the concentration of IgM(r=-0.185,P＜0.05).Reward and sleep quality were positively correlated with the concentration of C3 (r=0.172 and 0.285,both P ＜0.05),and negative affectivity was negatively correlated with the concentration of C3 (r=-0.174,P＜0.05);sleep quality was positively correlated with the concentration of C4 (r=0.173,P＜0.05),and negative affectivity was negatively correlated with the concentration of C4 (r=-0.164,P＜0.05).The logistic regression analysis revealed that the risks for a reduced concentration of IgG in the groups with high scores of social support and positive affectivity were 0.460 and 0.495 times those in the groups with low scores,while the risk for a reduced concentration of IgM in the group with a high score of coping strategy was 0.482 times that in the group with a low score.Conclusion Occupational stress is correlated with the serum levels of immunoglobulins and complement.

Objective To investigate the correlation of occupational stress with the serum levels of immunoglobulins(IgG,IgM,and IgA) and complement(C3 and C4).Methods In May 2011,convenience sampling and cluster sampling were used to select 225 policemen from a local police station as study subjects.A questionnaire was used to investigate demographic features and occupational stress,and the immunoturbidimetric assay was applied to measure the serum levels of IgG,IgM,IgA,and complement C3 and C4.Results Positive affectivity was positively correlated with the concentration of IgG(r=0.084,P＜0.01);positive affectivity,coping strategy,and sleep quality were positively correlated with the concentration of IgM (r=0.146,0.155,and 0.203,all P＜0.05);negative affectivity was negatively correlated with the concentration of IgM(r=-0.185,P＜0.05).Reward and sleep quality were positively correlated with the concentration of C3 (r=0.172 and 0.285,both P ＜0.05),and negative affectivity was negatively correlated with the concentration of C3 (r=-0.174,P＜0.05);sleep quality was positively correlated with the concentration of C4 (r=0.173,P＜0.05),and negative affectivity was negatively correlated with the concentration of C4 (r=-0.164,P＜0.05).The logistic regression analysis revealed that the risks for a reduced concentration of IgG in the groups with high scores of social support and positive affectivity were 0.460 and 0.495 times those in the groups with low scores,while the risk for a reduced concentration of IgM in the group with a high score of coping strategy was 0.482 times that in the group with a low score.Conclusion Occupational stress is correlated with the serum levels of immunoglobulins and complement.

Air Pollutants, Occupational ; analysis ; Butylene Glycols ; analysis ; Chromatography, Gas ; Workplace

OBJECTIVETo explore the relationship of occupational stressors with the serum levels of triiodothyronine (T3), thyroxine (T4), and thyroid stimulating hormone (TSH).

METHODSUsing convenience sampling and cluster sampling methods, 225 policemen from a local police station in China were enrolled as subjects. Questionnaires were used to investigate demographic features and occupational stressors in those subjects. The serum levels of T3, T4, and TSH were measured by radioimmunoassay. The SPSS 13.0 software was used to perform t test or analysis of variance, partial correlation analysis, and multivariate non-conditional logistic regression analysis.

RESULTSReward was positively correlated with the level of T3(P<0.05). Daily tension was positively correlated with the level of T4(P<0.05). Psychological demand, effort, and daily tension were negatively correlated with the level of TSH (all P<0.05). The quality of sleep was positively correlated with the level of TSH (P<0.05). The logistic regression analysis revealed that the risk of increase in T3 level in the group with a high score for daily tension was 3.19-fold higher than that in the group with a low score, while the risk of increase in T3 level in the group with a high score for negative emotion was 1.32-fold higher than that in the group with a low score. The risk of increase in TSH level in the group with a high score for negative emotion was 0.43-fold that in the group with a low score.

CONCLUSIONThe occupational stressors are correlated with the serum levels of thyroid hormones. Occupational stress can result in an increase in T3 level and a decrease in TSH level. However, occupational stress has no effect on T4 level.

China ; Humans ; Logistic Models ; Multivariate Analysis ; Police ; Stress, Psychological ; Surveys and Questionnaires ; Thyrotropin ; blood ; Thyroxine ; blood ; Triiodothyronine ; blood

OBJECTIVETo establish a method for simultaneously determining vanilmandelic acid (VMA), 5-hydroxyindoleacetic (5-HIAA), 3, 4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in urine by high-performance liquid chromatography (HPLC).

METHODSAfter being filtered with a 0.45 µm membrane syringe filter, the urinary samples were injected directly into the HPLC system using a C18 chromatographic column and a fluorescence detector. The excitation and emission wavelengths were chose as 280 nm and 315 nm, respectively, and the urinary samples were carried with a mobile phase of methanol-0.1 mol/L phosphate buffered solution (V/V = 20:80) at a flow rate of 1.0 ml/min and an injection volume of 20 µl.

RESULTSUsing the method reported here, the correlation coefficients of VMA, 5-HIAA, DOPAC, and HVA were 0.9999, 0.9998, 0.9997, 0.9999, respectively, over linear ranges of 0-2.5, 0-2.0, 0-2.0, and 0-2.5 µg/ml, the minimum detectable concentrations were 0.006, 0.008, 0.012, and 0.0082 µg/ml, the average precisions were 4.2%, 3.7%, 4.9%, and 3.6%, and the recovery rates were 91%∼102%, 93%∼101%, 94%∼101%, and 89%∼ 102%.

CONCLUSIONThis determination method is simple, efficient, accurate, and sensitive for the simultaneous detection of VMA, 5-HIAA, DOPAC, and HVA in urine.

Biogenic Amines ; urine ; Chromatography, High Pressure Liquid ; Homovanillic Acid ; urine ; Humans ; Hydroxyindoleacetic Acid ; urine ; Vanilmandelic Acid ; urine

OBJECTIVETo explore the possibility for 17-OHCS, 17-KS, VMA and HVA in urine as indicators of occupational stress identification and evaluation.

METHODS225 policies were investigated by using convenience sampling and cluster sampling methods, occupational stressors, strains, buffer factors and individual factors were measured by questionnaires. The urine 17-OHCS, 17-KS, VMA and HVA level was tested by ELISA and high performance liquid chromatography method respectively.

RESULTSThe urine 17-OHCS level of the groups with high coworker support and job control scores was lower than that of the groups with low score group (P < 0.05), the urine 17-OHCS level of the group with high negative affectivity score was higher than that of the group with low score group (P < 0.05). The urine 17-KS level of the group with high job security score was lower than that of the counterparts (P < 0.05). The urine VMA level of the group with high coworker support score was lower than that of the counterparts (P < 0.05). The urine HVA level of the group with high coping strategy score was lower than that of counterpart (P < 0.05). Logistic regression analysis revealed that the job control and respect were the protective factors of urine 17-OHCS level increase (OR = 0.353 and 0.352 respectively), but over-commitment, self-esteem and daily hassles were the risk factors of urine 17-OHCS with level increase (OR = 3.391, 2.439, and 2.584 respectively). The risk of urine 17-KS level increase for the groups high job control and job security scores were respectively 0.387, 0.356 times than that of the counterparts, the risk of urine VMA level increase for the group with high negative affectivity score was 2.643 times than that of the counterparts.

CONCLUSIONThe urine 17-OHCS, 17-KS and VMA level were potential indicators for occupational stress evaluation.

Adaptation, Psychological ; Adrenal Cortex Hormones ; urine ; Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; urine ; Risk Factors ; Stress, Psychological ; urine ; Surveys and Questionnaires ; Young Adult