OBJECTIVE To explore the distribution of pathogens isolated from respiratory tract secretions,observe the expressions of serum nitric oxide(NO),matrix metalloproteinase-2(MMP-2)and interleukin-8(IL-8)in the epiglottic cyst infection patients complicated with acute epiglottitis,and analyze the clinical significance.METHODS The clinical data were collected from 98 epiglottic cyst infection patients complicated with acute epi-glottitis who were treated in the First People's Hospital of Linping District from Jan.2019 to Jun.2023.The dis-tribution of pathogens isolated from the respiratory tract secretions was recorded.Among the enrolled patients,45 patients who received conservative therapy were assigned as the non-surgery group,and 53 patients who under-went electrotome resection under self-retaining laryngoscope were assigned as the surgery group.The levels of ser-um NO,MMP-2 and IL-8 were compared between the two groups of patients,the recurrence rate was observed and compared after the discharge for 6 months.The values of serum NO,MMP-2 and IL-8 at the admission in prediction of recurrence of the non-surgery group 6 months after the surgery were analyzed by means of receiver operating characteristic(ROC)curves.RESULTS Totally 61 strains of pathogens were isolated from the 98 pa-tients,63.93％of which were gram-negative bacteria,and 36.07％were gram-positive bacteria.The levels of ser-um NO,MMP-2 and IL-8 of the two groups were lower after the discharge than at the admission(P＜0.05),which were lower on the 7th day of admission than on the 3rd day of admission(P＜0.05).The levels of serum NO,MMP-2 and IL-8 of the surgery group were lower than those of the non-surgery group on the 7th day of dis-charge(P＜0.05).The recurrence rate of the surgery group was 0 after the discharge for 6 months,lower than 13.33％of the non-surgery group(0.00％vs.13.33％,continuity correction x2=5.387,P=0.020).ROC curve a-nalysis showed that the levels of serum NO,MMP-2 and IL-8 at the admission had statistical significance in pre-diction of recurrence 6 months after the discharge(P＜0.05).CONCLUSION As compared with conservative thera-py,the electrotome resection under self-retaining laryngoscope may reduce the recurrence rate of acute epiglottitis in the epiglottic cyst infection patients,and the levels of serum NO,MMP-2 and IL-8 can assist the prediction of risk of recurrence.

OBJECTIVE To explore the distribution of pathogens isolated from respiratory tract secretions,observe the expressions of serum nitric oxide(NO),matrix metalloproteinase-2(MMP-2)and interleukin-8(IL-8)in the epiglottic cyst infection patients complicated with acute epiglottitis,and analyze the clinical significance.METHODS The clinical data were collected from 98 epiglottic cyst infection patients complicated with acute epi-glottitis who were treated in the First People's Hospital of Linping District from Jan.2019 to Jun.2023.The dis-tribution of pathogens isolated from the respiratory tract secretions was recorded.Among the enrolled patients,45 patients who received conservative therapy were assigned as the non-surgery group,and 53 patients who under-went electrotome resection under self-retaining laryngoscope were assigned as the surgery group.The levels of ser-um NO,MMP-2 and IL-8 were compared between the two groups of patients,the recurrence rate was observed and compared after the discharge for 6 months.The values of serum NO,MMP-2 and IL-8 at the admission in prediction of recurrence of the non-surgery group 6 months after the surgery were analyzed by means of receiver operating characteristic(ROC)curves.RESULTS Totally 61 strains of pathogens were isolated from the 98 pa-tients,63.93％of which were gram-negative bacteria,and 36.07％were gram-positive bacteria.The levels of ser-um NO,MMP-2 and IL-8 of the two groups were lower after the discharge than at the admission(P＜0.05),which were lower on the 7th day of admission than on the 3rd day of admission(P＜0.05).The levels of serum NO,MMP-2 and IL-8 of the surgery group were lower than those of the non-surgery group on the 7th day of dis-charge(P＜0.05).The recurrence rate of the surgery group was 0 after the discharge for 6 months,lower than 13.33％of the non-surgery group(0.00％vs.13.33％,continuity correction x2=5.387,P=0.020).ROC curve a-nalysis showed that the levels of serum NO,MMP-2 and IL-8 at the admission had statistical significance in pre-diction of recurrence 6 months after the discharge(P＜0.05).CONCLUSION As compared with conservative thera-py,the electrotome resection under self-retaining laryngoscope may reduce the recurrence rate of acute epiglottitis in the epiglottic cyst infection patients,and the levels of serum NO,MMP-2 and IL-8 can assist the prediction of risk of recurrence.

Humans ; Proto-Oncogene Proteins p21(ras)/genetics* ; Prognosis ; Biomarkers, Tumor/genetics* ; Mutation/genetics* ; Colorectal Neoplasms/genetics* ; Proto-Oncogene Proteins B-raf/metabolism*

Objective To investigate the clinical efficacy of intratympanic corticosteroids(ITS)and hyperbaric oxygen therapy(HBOT)in patients with idiopathic sudden sensorineural hearing loss(ISSNHL)who had failed systemic corticosteroid(SS)treatment.Methods ISSNHL patients admitted to the Department of Otolaryngology,First People's Hospital of Linping District of Hangzhou city from January 2020 to January 2023 were retrospectively collected.According to the treatment plan,patients with ISSNHL were divided into SS group,HBOT group,and ITS group.The changes in hearing level[purity hearing threshold mean(PTA)]and hearing gain of ISSNHL patients before treatment(T0),day 5(T1)and after 3 months of treatment(T2)were observed and compared,and the total effective rate of treatment in different groups was compared.Univariate analysis was used to explore the influencing factors of the efficacy of patients with different treatment modalities of ISSNHL.Results A total of 156 ISSNHL patients were included,including 70 in the SS group,33 in the HBOT group and 53 in the ITS group.At T0,there was no significant difference in PTA among the three groups of patients(P>0.05).At T2,the level of PTA in the three groups of ISSNHL patients was significantly lower than that before treatment(P<0.05).The levels of PTA in the HBOT group and ITS group were significantly lower than those in the SS group(P<0.05).The level of PTA in the HBOT group was significantly lower than that in the ITS group(P<0.05).The hearing gain of the HBOT group and the ITS group was significantly higher than that of the SS group(P<0.05),and the hearing gain of the HBOT group was significantly higher than that of the ITS group(P<0.05).The total effective rate of HBOT group was significantly higher than that of ITS group(P<0.05),and the total effective rate of ITS group was significantly higher than that of SS group(P<0.05).In addition,vertigo was an influencing factor for poor hearing recovery in ISSNHL patients regardless of treatment regimen(P<0.05).Conclusion Both ITS and HBOT can improve the treatment efficiency of ISSNHL patients who have failed SS treatment and promote hearing recovery in ISSNHL.Compared with IST,HBOT has more significant efficacy,and it is recommended that clinical treatment be prioritized.Vertigo is an influencing factor for poor hearing recovery in patients with ISSNHL who have failed SS treatment,and should be focused on in clinical practice.

Objective To isolate endophytic fungi from Angelica sinensis and evaluate the bioactivity of their secondary metabolites.Methods Angelica sinensis and rhizosphere soil were utilized as materials.The tissue homogenization method was employed with six diverse culture media to isolate endophytic fungi.The antibacterial activity of secondary metabolites was gauged using a 96-well plate assay,while UV spectrophotometry was used to evaluate the inhibitory activity of four enzymes.Results A total of 153 fungal strains were isolated and purified from Angelica sinensis roots,stems,leaves,and soil.The samples exhibited specific inhibitory activities against adenosine deaminase(ADA),β-lactamase,xanthine oxidase(XO),and tyrosinase(TYR),with rates of 45.83%,52.78%,51.39%and 55.56%,respectively.Furthermore,1.39%of the samples displayed wide-ranging inhibitory effects against four indicator bacteria.Strain 6B also showcased the lowest inhibitory concentration values of 62.5 and 7.81 μg/mL against Escherichia coli ATCC25922 and ATCC35218,respectively,signifying its potential research significance.Conclusion Angelica sinensis has abundant endophytic fungal resources and is a good source for discovering active compounds,demonstrating certain research value.

Objective To establish a nomogram model for efficiently predicting overall survival(OS)and cancer-specific survival(CSS)in patients with CRCHBM.Method 2239 patients from 2010 to 2019 were retrospectively analyzed from the Surveillance,Epidemiology,and End Results Program(SEER)databases and Wuhan Union Hospital Cancer Center.SEER is randomly assigned to the training and internal validation cohorts,and the Wuhan database serves as the external validation.Cox regression analyses were used to determine the independent clinicopathological prognosis factors affecting OS and CSS,and a nomogram was constructed to predict OS and CSS.The clinical utility of columnar plots was assessed using calibration curves,area under the curve(AUC),and decision curve analysis(DCA).Result OS column line graphs were constructed based on nine independent predictors:age,tumor location,degree of differentiation,tumor size,TNM stage,chemotherapy,primary focus surgery,number of lymph nodes sampled,and serum carcinoembryonic antigen(CEA)level.The C-index of the nomogram to predict the 1-,3-,and 5-year OS were 0.764,0.790,and 0.805 in the training group,0.754,0.760,and 0.801 in the internal validation group,and 0.822,0.874,and 0.906 in the external validation group.CSS column line graphs were constructed based on 3 independent predictors of TNM staging,radiotherapy and chemotherapy.The 1-,3-,and 5-year CSS AUROC values of the training group were 0.791,0.757,and 0.782,respectively.0.682,0.709,0.625 in the internal validation group and 0.759,0.702,0.755 in the external validation group,respectively.The results of receiver operating characteristic curve(ROC),ROC and DCA showed that the use of our model was more effective in predicting OS and CSS than other single clinicopathological features.Conclusion In summary,the nomogram based on significant clinicopathological features can be conveniently used to predict OS and CSS individually in patients with CRCHBM.

The problem of single educational origin structure generally existed in affiliated hospitals of universities, which could not only affect the academic vitality and scientific research innovation ability of the hospital, but also may lead to restricted discipline development. Through measures such as reforming the hospital-retention policy, expanding recruitment channels, strengthening talent cultivation and formulating supporting policies, Union Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology has successfully increased the proportion of doctors graduated from other hospitals, promoted the diversification of educational origin structure. The author suggested that affiliated hospitals of universities should establish a diversified human resources allocation system during the recruitment stage, give support policies to employees graduated from other hospitals, pay attention to the balanced development of sub-specialties, and expand the educational origin of existing employees, so as to promote the healthy development of clinical disciplines.

The problem of single educational origin structure generally existed in affiliated hospitals of universities, which could not only affect the academic vitality and scientific research innovation ability of the hospital, but also may lead to restricted discipline development. Through measures such as reforming the hospital-retention policy, expanding recruitment channels, strengthening talent cultivation and formulating supporting policies, Union Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology has successfully increased the proportion of doctors graduated from other hospitals, promoted the diversification of educational origin structure. The author suggested that affiliated hospitals of universities should establish a diversified human resources allocation system during the recruitment stage, give support policies to employees graduated from other hospitals, pay attention to the balanced development of sub-specialties, and expand the educational origin of existing employees, so as to promote the healthy development of clinical disciplines.