ObjectiveTo investigate whether Huanglian Jiedutang （HLJD） and its major active constituents （geniposide， baicalin， and berberine） can inhibit the inflammatory response of BV2 cells under lipopolysaccharide （LPS） stimulation via the high-mobility group protein B1 （HMGB1）/Toll-like receptor 4 （TLR4）/nuclear factor-κB （NF-κB） signaling pathway， and to explore differences in therapeutic efficacy among the three monomers， their combined formula， and HLJD under equal content ratios. MethodsBV2 microglial cells were used as the primary experimental model. Cell viability was assessed using the cell counting kit-8 （CCK-8） method to examine the effects of different concentrations of dimethyl sulfoxide （DMSO， 0.8%， 0.4%， 0.2%， 0.1%， and 0.05%） on cell viability. IncuCyte was employed to monitor the growth of cells under different concentrations of HLJD （200， 100， 50， 25， 12.5， 6.25 mg·L^-1）. Nitric oxide （NO） assay was used to screen the optimal HLJD concentration. High-performance liquid chromatography （HPLC） determined the content of geniposide， baicalin， and berberine in HLJD， and experimental groups were subsequently established according to the relative proportions of these constituents. CCK-8 assay evaluated cell viability under different treatments. Enzyme-linked immunosorbent assay （ELISA） measured levels of inflammatory factors （TNF-α， IL-1β， IL-6， IL-10） in the supernatant. Flow cytometry assessed the effects of treatments on M1-type polarization of BV2 cells. Western blot determined the expression levels of HMGB1， TLR4， and NF-κB-related proteins. ResultsCompared with the blank group， DMSO at concentrations ≤0.2% did not affect cell viability within 48 h. BV2 cell growth plateaued at 24 h after treatment with 200 mg·L^-1 HLJD. Under stimulation with 2 mg·L^-1 LPS， this concentration of HLJD effectively reduced NO release， and 6 h pre-treatment had a stronger inhibitory effect on NO than direct administration. HPLC results showed that 1 mg of HLJD freeze-dried powder contained approximately 24 μg of geniposide， 15 μg of baicalin， and 30 μg of berberine. Based on these ratios， experimental groups were blank， LPS （2 mg·L^-1）， HLJD （200 mg·L^-1）， monomer combination， geniposide （4.8 mg·L^-1）， baicalin （3 mg·L^-1）， and berberine （6 mg·L^-1）. The monomer combination group consisted of all three active constituents dissolved together. LPS and HLJD or its active constituents did not affect cell viability compared with the blank group. LPS significantly increased TNF-α， IL-1β， IL-6， and IL-10 in the supernatant （P<0.01）. HLJD and its active constituents significantly reduced pro-inflammatory factors TNF-α， IL-1β， and IL-6 （P<0.05， P<0.01） while upregulating anti-inflammatory IL-10 （P<0.01）， with the monomer combination showing the strongest effect （P<0.05， P<0.01）. Compared with the blank group， LPS significantly increased the proportion of CD80⁺CD86⁺ （M1-type） BV2 cells （P<0.01）. HLJD and its constituents partially inhibited M1 polarization （P<0.05， P<0.01）， with the monomer combination exhibiting the most pronounced effect （P<0.05， P<0.01）. Compared with the blank group， LPS upregulated HMGB1， TLR4， and NF-κB-related proteins （P<0.01）， whereas HLJD and its active constituents significantly reduced their expression （P<0.05， P<0.01）， with the monomer combination having the strongest regulatory effect （P<0.05， P<0.01）. ConclusionHLJD and its major active constituents （geniposide， baicalin， berberine） can inhibit LPS-induced inflammatory responses in BV2 cells. The combination of the three active constituents demonstrates the most potent anti-inflammatory effect， significantly attenuating M1-type polarization of BV2 cells via the HMGB1/TLR4/NF-κB signaling pathway.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective To explore public emergency medical rescue resources'satisfaction of demand,influencing factors and multi-subject enabling countermeasures under the flood disaster.Methods A total of 651 valid question-naires were collected from victims in four towns in one city.Logistic regression analysis was applied to analyze the in-fluencing factors.Results 80.49％of the respondents considered that their and their families'emergency medical res-cue resources'demand could be met during the flood.The results of logistic regression analysis showed influencing factors of low satisfaction including there were the elderly aged 65 and above in the family,and patients with chronic diseases such as diabetes and hypertension in the family and the differences were statistically significant(P＜0.05);Influencing factors of high satisfaction including adequate preparation of familial emergency supplies,the grassroots government publicized the disaster prevention and self-help knowledge before the disaster,the grass-roots government had prepared emergency medical rescue resources,such as food,water and relief materials be-fore the disaster,the adequacy of emergency medical rescue resources of local hospital,and medical staff pro-moted psychological knowledge and skill and the differences were statistically significant(P＜0.05).Conclusion The store of emergency resource should be strengthened in all aspects,including medical institutions,the grass-roots governments and the public in the community,and emergency preparedness and publicity work should be promoted in a coordinated manner,so as to build a resilient community and society for disaster response.

Objective:To investigate the efficacy and safety of different treatment duration of dual therapy including vonoprazan and low-dose amoxicillin in Helicobacter pylori ( H. pylori) eradication. Methods:From December 1, 2022 to November 30, 2023, a total of 150 H. pylori-infected patients who underwent primary treatment in the Affiliated Changzhou No.2 People′s Hospital of Nanjing Medical University were recruited. The patients were divided into 10-day treatment group and 14-day treatment group with 75 patients in each group by random number table method. Patients of both groups were treated with vonoprazan (20 mg each time, twice per day) combined with amoxicillin (0.75 g each time, 3 times per day), and the treatment duration was 10 and 14 days, respectively. Intention-to-treat (ITT) and per-protocol (PP) analyses were used to evaluate the eradication efficacy of H. pylori of the 10-day treatment group and 14-day treatment group. PP analysis was used to compare the incidence of adverse events between the 2 groups. Cost-effectiveness analysis was performed to evaluate the economic efficiency of the 2 treatment regimens. Identified factors affecting the eradication rate in the 10-day treatment group based on the PP. Independent sample t-test and chi-square test were used for statistical analysis. Results:In ITT and PP analysis, a total of 75, 75 patients and 70, 72 patients were included in the 10-day treatment group and 14-day treatment group, respectively. The results of ITT analysis showed that the eradication rates of 10-day treatment group and 14-day treatment group were 86.7% (65/75) and 90.7% (68/75), respectively. The results of PP analysis showed that the eradication rates of the 2 groups were 92.9% (65/70) and 94.4% (68/72), respectively, and the differences were not statistically significant (both P>0.05). The results of PP analysis showed that the incidences of adverse events of the 10-day treatment group and 14-day treatment group were 12.9% (9/70) and 9.7% (7/72), respectively, and the difference was not statistically significant( P>0.05). The cost-effectiveness ratios of the 10-day treatment group and 14-day treatment group were 3.29 and 4.19 yuan/%, respectively. The optimal cut-off values were 63.5 kg for body weight and 1.73 m 2 for body surface area to affect the H. pylori eradication rate. The H. pylori eradication rate of patients with body weight ≤ 63.5 kg was higher than that of patients with body weight >63.5 kg (100.0%, 42/42 vs. 82.1%, 23/28), and the H. pylori eradication rate of the patients with body surface area≤1.73 m 2 was higher than that of patients with body surface area>1.73 m 2 (100.0%, 45/45 vs. 80.3%, 20/25), and the differences were statistically significant ( χ2=5.61 and 6.91, P=0.018 and 0.009). Conclusions:The 10-day combination therapy of vonoprazan and low-dose amoxicillin is a safe and effective primary treatment regimen for H. pylori eradication. Body weight and body surface area are influencing factors of the eradication rate.