Objective To observe the value of intratumoral tertiary lymphoid structure(TLS)for predicting prognosis of soft tissue sarcomas(STS)after cryoablation combined with immunotherapy.Methods Forty-three STS patients who underwent cryoablation were retrospectively enrolled,including 38 cases underwent cryoablation combined with but 5 cases without immunotherapy,among them 6 cases with but 37 cases without intratumoral TLS.The progression free survival(PFS)were compared between those with and without immunotherapy,as well as those with and without intratumoral TLS.The value of intratumoral TLS for predicting prognosis of STS after cryoablation combined with immunotherapy was analyzed with Cox regression and receiver operating characteristic curves.Results No significant difference of PFS was found between STS patients undwerwent cryoablation combined with and without immunotherapy(P＜0.05).Among 38 cases underwent cryoablation combined with immunotherapy,6 cases were found with but 32 cases without intratumoral TLS,and the median PFS of the former was longer than that of the latter(6 months vs.3 months,P＜0.05).Univariate and multivariate Cox regression analysis revealed that intratumoral TLS and interleukin 2 receptor(IL-R2)were both independent influencing factors of PFS of STS patients after cryoablation combined with immunotherapy(both P＜0.05).The area under the curve(AUC)of intratumoral TLS for predicting PFS of STS patients at 3,6 and 9 months was 0.550,0.577 and 0.699,of IL-R2 was 0.658,0.742 and 0.583,respectively,while of combination of the above two was 0.762,0.787 and 0.881,respectively,all higher than of each intratumoral TLS and IL-R2 alone(all P＜0.05).Conclusion Intratumoral TLS could be used to predict prognosis of STS after cryoablation combined with immunotherapy.Combining with IL-R2 might further improve its predictive efficacy.

Objective To assess potential associations between sarcoma and gut microbiota using Mendelian randomization(MR)analysis.Methods Instrumental variables(IV)for gut microbiota were identified from summary data of a genome-wide association studies(GWAS)involving 18 340 participants from 24 cohorts published by the MiBioGen Consortium.The GWAS on sarcoma from Europe served as an outcome,involving 456 276 sarcoma patients.The inverse-variance weighted(IVW)method was used for primary analysis while sensitivity analysis was made to test the reliability of results of MR analysis.Results IVW results showed that Peptostreptococcaceae(OR=0.353,95％CI 0.127 to 0.979,P=0.045)and Faecalibacterium(OR=0.408,95％CI 0.173 to 0.961,P=0.040)had a negative causal correlation with sarcoma,but the Eubacterium fissicatena group(OR=2.216,95％CI 1.101 to 4.462,P=0.026)and Lachnospiraceae UCG008(OR=1.822,95％CI 1.005 to 3.305,P=0.048)exhibited a positive causal correlation with sarcoma.Sensitivity analysis showed no evidence of pleiotropy and heterogeneity.Conclusion Through two-sample MR,this study identifies a potential causal relationship between four gut microbiota and sarcoma.Peptostreptococcaceae and Faecalibacterium can reduce the risk of sarcoma while the Eubacterium fissicatena group and Lachnospiraceae UCG008 are linked to an increased risk of sarcoma.More research is required to find out more about the way gut microbiota influences the development of sarcoma.

Objective To observe the value of intratumoral tertiary lymphoid structure(TLS)for predicting prognosis of soft tissue sarcomas(STS)after cryoablation combined with immunotherapy.Methods Forty-three STS patients who underwent cryoablation were retrospectively enrolled,including 38 cases underwent cryoablation combined with but 5 cases without immunotherapy,among them 6 cases with but 37 cases without intratumoral TLS.The progression free survival(PFS)were compared between those with and without immunotherapy,as well as those with and without intratumoral TLS.The value of intratumoral TLS for predicting prognosis of STS after cryoablation combined with immunotherapy was analyzed with Cox regression and receiver operating characteristic curves.Results No significant difference of PFS was found between STS patients undwerwent cryoablation combined with and without immunotherapy(P＜0.05).Among 38 cases underwent cryoablation combined with immunotherapy,6 cases were found with but 32 cases without intratumoral TLS,and the median PFS of the former was longer than that of the latter(6 months vs.3 months,P＜0.05).Univariate and multivariate Cox regression analysis revealed that intratumoral TLS and interleukin 2 receptor(IL-R2)were both independent influencing factors of PFS of STS patients after cryoablation combined with immunotherapy(both P＜0.05).The area under the curve(AUC)of intratumoral TLS for predicting PFS of STS patients at 3,6 and 9 months was 0.550,0.577 and 0.699,of IL-R2 was 0.658,0.742 and 0.583,respectively,while of combination of the above two was 0.762,0.787 and 0.881,respectively,all higher than of each intratumoral TLS and IL-R2 alone(all P＜0.05).Conclusion Intratumoral TLS could be used to predict prognosis of STS after cryoablation combined with immunotherapy.Combining with IL-R2 might further improve its predictive efficacy.