1.Effect of Acupuncture with the Method of Soothing the Liver and Regulating the Mind on the TLR4/NF-κB Pathway in the Hippocampus of Lipopolysaccharide-Induced Depression Model Mice
Bingxin WU ; Yi ZHOU ; Sibo HAN ; Xichang HUANG ; Junye MA ; Baile NING ; Shanze WANG ; Wenbin FU
Journal of Traditional Chinese Medicine 2025;66(5):526-531
ObjectiveTo explore the potential mechanism of acupuncture with the method of soothing the liver and regulating the mind in improving depressive disorder. MethodsEighteen C57BL/6J mice were randomly divided into blank group, model group, and acupuncture group, with 6 mice in each group. The model group and the acupuncture group were subjected to depression induction by intraperitoneal injection of lipopolysaccharide (LPS), while the blank group received an equal volume of normal saline once daily for seven consecutive days. Concurrently, the acupuncture group received "soothing the liver and regulating the mind" acupuncture intervention starting from the first day of modeling, once daily for 14 days; whereas the blank group and the model group were only restrained without acupuncture. The sucrose preference test was used to assess sucrose preference rate, the open-field test to measure center stay time and total travel distance, and the forced swim test to evaluate immobility time. Hematoxylin-eosin (HE) staining was performed to observe hippocampal morphological changes. Enzyme-linked immunosorbent assay (ELISA) was used to detect levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in hippocampal tissue. Western blot analysis was conducted to examine the protein expression levels of Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) in the hippocampus. ResultsCompared to the blank group, the model group showed a significant reduction in sucrose preference rate, center stay time, and total travel distance, along with a significant increase in immobility time in the forced swim test, hippocampal IL-1β, IL-6, and TNF-α levels, as well as TLR4 and NF-κB protein expression (P<0.01), and the histological examination revealed blurred hippocampal neuronal boundaries, loose arrangement, and some neurons exhibiting nuclear pyknosis and deep staining. Compared to the model group, the acupuncture group demonstrated a significant increase in sucrose preference rate, center stay time, and total travel distance, along with a significant reduction in immobility time in the forced swim test, hippocampal IL-1β, IL-6, and TNF-α levels, and TLR4 and NF-κB protein expression (P<0.01), and the histological analysis showed that hippocampal neurons in the acupuncture group were more tightly arranged, with reduced nuclear pyknosis and deep staining. ConclusionAcupuncture with the "soothing the liver and regulating the mind" method can significantly improve depression-like behavior, potentially by inhibiting the hippocampal TLR4/NF-κB signaling pathway and alleviating inflammatory responses.
2.A survey on retraining desire and demand of specialist nurses in 4 563 medical institutions in China
Yanmeng GUAN ; Junye TIAN ; Yanling WANG ; Yanming DING
Chinese Journal of Nursing 2025;60(2):223-227
Objective To investigate the desire and demand for the retraining of specialist nurses medical institutions in China,and to provide references for the further work of the re-certification of specialist nurses.Methods Convenient sampling method was used to select the head nurses in the nursing departments of 5 030 medical institutions in 30 provinces,autonomous regions and municipalities as the survey subjects in May 2023.The questionnaire was self-designed,and the survey was conducted by anonymous electronic questionnaire.Results 4 563 valid questionnaires were collected.Among the 4 563 medical institutions,the training of specialized nurses was carried out in various fields,among which 46.31%of the medical institutions have carried out the training of intensive care specialized nurses.82.29%of the medical institutions considered it necessary to retrain specialized nurses;60.86%of the medical institutions suggested that the training cycle should be once every 5 years;68.05%of the medical institutions suggested that the training method should be online;47.97%of the medical institutions thought that the conditions could be applied without limitation.More than 80%of medical institutions believe that specialized knowledge and clinical practice ability should be the main training and assessment content.Conclusion Most medical institutions have the need for retraining of specialist nurses,and there are differentiated needs for the implementation of retraining.Retraining can be carried out on a pilot basis in the field of intensive care.The retraining and assessment of specialist nurses should mainly focus on specialist knowledge and clinical practice ability.
3.Exploring the acupuncture and moxibustion diagnosis and treatment approach of bipolar disorder from the perspective of"wood element characterized by spreading harmony"
Junye MA ; Zengyan LI ; Lei WANG ; Weisheng HU ; Yueyue YANG ; Wenbin FU
Journal of Beijing University of Traditional Chinese Medicine 2025;48(10):1339-1344
The phrase"wood element characterized by spreading harmony"from the Huangdi Neijing five-phase theory summarizes the fundamental nature and functions of the wood element,emphasizing the crucial significance of the harmonious virtue of wood in promoting the generation and transformation of all things.In human physiology,"wood element characterized by spreading harmony"corresponds to the harmonious spreading of liver-gallbladder qi,ensuring smooth liver qi regulation and proper functioning of the ascending-descending movement mechanisms.Bipolar disorder,categorized in traditional Chinese medicine as"depression"or"manic depressive psychosis,"primarily arises from hepatic wood dysfunction.Insufficient wood qi dispersion typically manifests as a depressive phase,whereas excessive dispersion leads to a manic phase,with alternating manic-depressive phase indicating qi movement disorder.By integrating wood qi characteristics,disease pathogenesis of bipolar disorder,and our team's clinical experience in treating emotional disorders,this study developed an integrated acupuncture and moxibustion diagnosis and treatment approach for bipolar disorder,focusing on soothing the liver and regulating spirit,structured as"acupuncture-moxibustion-consolidation."Based on the physiological characteristics of"wood element characterized by spreading harmony,"from liver failing to controll conveyance and dispersion:depressive phase should apply warm-nourishing growth method,manic phase should suppress hyperactive liver for tranquillizing mind method,and alternating manic-depressive phase should apply harmonized rigidity-flexibility method.These methods aim to regulate the spirit of the heart and brain,harmonize the qi movement of the spleen and stomach,and replenish the essence and blood of the liver and kidney.Clinical application through syndrome differentiation has demonstrated satisfactory therapeutic outcomes.
4.Expert consensus on perioperative clinical nursing standards for patients undergoing percutaneous renal biopsy (2024 edition)
Yan WANG ; Junye TIAN ; Yuan HAN ; Liyun CAO ; Fude ZHOU ; Ruxia WANG ; Yanmeng GUAN ; Dong PANG ; Jingfen JIN ; Yanming DING ; Ting CHEN ; Xiaohong YIN ; Jing HUANG ; Dengyan MA ; Jianying LI ; Chunyue LI
Chinese Journal of Modern Nursing 2025;31(19):2521-2528
To provide scientific guidance and standardization for perioperative clinical nursing practice in patients undergoing percutaneous renal biopsy, relevant evidence on percutaneous renal biopsy nursing care was systematically retrieved and synthesized through an evidence-based approach. After two rounds of Delphi expert consultation and expert panel discussions, expert consensus on perioperative clinical nursing standards for patients undergoing percutaneous renal biopsy (hereinafter referred to as the "Consensus") was developed. The Consensus includes three primary themes: preoperative care, intraoperative care, and postoperative care, encompassing 21 secondary themes. It is characterized by scientific rigor, practical applicability, and comprehensiveness, and serves as a valuable reference and guide for clinical nursing professionals across medical institutions.
5.Exploring the acupuncture and moxibustion diagnosis and treatment approach of bipolar disorder from the perspective of"wood element characterized by spreading harmony"
Junye MA ; Zengyan LI ; Lei WANG ; Weisheng HU ; Yueyue YANG ; Wenbin FU
Journal of Beijing University of Traditional Chinese Medicine 2025;48(10):1339-1344
The phrase"wood element characterized by spreading harmony"from the Huangdi Neijing five-phase theory summarizes the fundamental nature and functions of the wood element,emphasizing the crucial significance of the harmonious virtue of wood in promoting the generation and transformation of all things.In human physiology,"wood element characterized by spreading harmony"corresponds to the harmonious spreading of liver-gallbladder qi,ensuring smooth liver qi regulation and proper functioning of the ascending-descending movement mechanisms.Bipolar disorder,categorized in traditional Chinese medicine as"depression"or"manic depressive psychosis,"primarily arises from hepatic wood dysfunction.Insufficient wood qi dispersion typically manifests as a depressive phase,whereas excessive dispersion leads to a manic phase,with alternating manic-depressive phase indicating qi movement disorder.By integrating wood qi characteristics,disease pathogenesis of bipolar disorder,and our team's clinical experience in treating emotional disorders,this study developed an integrated acupuncture and moxibustion diagnosis and treatment approach for bipolar disorder,focusing on soothing the liver and regulating spirit,structured as"acupuncture-moxibustion-consolidation."Based on the physiological characteristics of"wood element characterized by spreading harmony,"from liver failing to controll conveyance and dispersion:depressive phase should apply warm-nourishing growth method,manic phase should suppress hyperactive liver for tranquillizing mind method,and alternating manic-depressive phase should apply harmonized rigidity-flexibility method.These methods aim to regulate the spirit of the heart and brain,harmonize the qi movement of the spleen and stomach,and replenish the essence and blood of the liver and kidney.Clinical application through syndrome differentiation has demonstrated satisfactory therapeutic outcomes.
6.A survey on retraining desire and demand of specialist nurses in 4 563 medical institutions in China
Yanmeng GUAN ; Junye TIAN ; Yanling WANG ; Yanming DING
Chinese Journal of Nursing 2025;60(2):223-227
Objective To investigate the desire and demand for the retraining of specialist nurses medical institutions in China,and to provide references for the further work of the re-certification of specialist nurses.Methods Convenient sampling method was used to select the head nurses in the nursing departments of 5 030 medical institutions in 30 provinces,autonomous regions and municipalities as the survey subjects in May 2023.The questionnaire was self-designed,and the survey was conducted by anonymous electronic questionnaire.Results 4 563 valid questionnaires were collected.Among the 4 563 medical institutions,the training of specialized nurses was carried out in various fields,among which 46.31%of the medical institutions have carried out the training of intensive care specialized nurses.82.29%of the medical institutions considered it necessary to retrain specialized nurses;60.86%of the medical institutions suggested that the training cycle should be once every 5 years;68.05%of the medical institutions suggested that the training method should be online;47.97%of the medical institutions thought that the conditions could be applied without limitation.More than 80%of medical institutions believe that specialized knowledge and clinical practice ability should be the main training and assessment content.Conclusion Most medical institutions have the need for retraining of specialist nurses,and there are differentiated needs for the implementation of retraining.Retraining can be carried out on a pilot basis in the field of intensive care.The retraining and assessment of specialist nurses should mainly focus on specialist knowledge and clinical practice ability.
7.Expert consensus on perioperative clinical nursing standards for patients undergoing percutaneous renal biopsy (2024 edition)
Yan WANG ; Junye TIAN ; Yuan HAN ; Liyun CAO ; Fude ZHOU ; Ruxia WANG ; Yanmeng GUAN ; Dong PANG ; Jingfen JIN ; Yanming DING ; Ting CHEN ; Xiaohong YIN ; Jing HUANG ; Dengyan MA ; Jianying LI ; Chunyue LI
Chinese Journal of Modern Nursing 2025;31(19):2521-2528
To provide scientific guidance and standardization for perioperative clinical nursing practice in patients undergoing percutaneous renal biopsy, relevant evidence on percutaneous renal biopsy nursing care was systematically retrieved and synthesized through an evidence-based approach. After two rounds of Delphi expert consultation and expert panel discussions, expert consensus on perioperative clinical nursing standards for patients undergoing percutaneous renal biopsy (hereinafter referred to as the "Consensus") was developed. The Consensus includes three primary themes: preoperative care, intraoperative care, and postoperative care, encompassing 21 secondary themes. It is characterized by scientific rigor, practical applicability, and comprehensiveness, and serves as a valuable reference and guide for clinical nursing professionals across medical institutions.
8.Construction of the User Portrait Model of Internet Medical Platform Based on Text Mining
Yanhua LYU ; Kanglong WANG ; Xiaoyun ZHONG ; Junye CHEN
Journal of Medical Informatics 2024;45(6):7-12
Purpose/Significance The internet consultation user portrait is constructed to explore the consultation topic,improve the consultation service quality,reduce the communication barriers between doctors and patients,and provide targeted treatment in an online and offline manner.Method/Process Python crawler is used to obtain the autism diagnosis data of a medical platform,and the combined model of LDA and TF-TFIDF is used to divide the data,and the user group classification is realized after dimensionality reduction clus-tering.Finally,the characteristic sets of different user groups are calculated and output by logistic regression model to construct the por-trait.Result/Conclusion The consultation content of users mainly focuses on 11 topics.The platform can optimize the consultation filling template based on the typical characteristics of the subject content to improve the accuracy of the disease description,consultation effi-ciency and satisfaction of patients.
9.Analysis of the Evolution of Themes in Traditional Chinese Medicine Policies in China Based on the LDA Model
Lian WANG ; Yongxu YUAN ; Junye CHEN ; Caiming YIN ; Yifan SUN
Journal of Medical Informatics 2024;45(9):44-49,62
Purpose/Significance To analyze the text content of traditional Chinese medicine(TCM)policies issued by the central govern-ment in China,to identify hot topics,and to carry out thematic evolution analysis.Method/Process The paper retrieves TCM policies issued in China from 2006 to 2023,applies LDA theme model for theme extraction,hotspot analysis,and theme evolution analysis for each policy stage.Result/Conclusion TCM policies in China focus on clinical medical services,scientific research and innovation,education development,industrial construction,and cultural transmission.The hotspots of these policies exhibit unique characteristics at different stages.The evolution of TCM policies in our country shows a trend towards basic construction standardization,refinement,and comprehensive development.
10.Analysis of Hotspots of Domestic Community Elderly Care Services Based on LDA Model and Life Cycle Theory
Caiming YIN ; Yongxu YUAN ; Lian WANG ; Yifan SUN ; Junye CHEN
Journal of Medical Informatics 2024;45(10):47-52,97
Purpose/Significance To review the existing literature,and to analyze the main research directions of community elderly care services,so as to provide scientific suggestions for improving community elderly care services.Method/Process The literature data are obtained from CNKI database and combined with the life cycle theory,the related research process is divided into 3 stages.The topic changes in each stage are identified by LDA model,and the research hotspots and development trends in this field are analyzed and opin-ions are put forward.Result/Conclusion The researches on domestic community elderly care services focus on the elderly care mode,service system construction,rural and community services,aiming to provide comprehensive and professional services,improve service quality,and meet the diverse needs of the elderly.Community elderly care service is still a hot spot of social concern.In the future,we should strengthen service evaluation,pay attention to talent construction,and make full use of digital technology in this field.

Result Analysis
Print
Save
E-mail