The infectious clone plasmid of genotype Ⅰ Japanese encephalitis virus GX strain was suc-cessfully obtained by reverse transcription-polymerase chain reaction,where the cDNA of GX strain is divided into three fragments for amplification and the three fragments were sequentially cloned into pBR322 vector.After the infectious clone plasmid pGX was sequenced correctly,the pGX and pCAGGS-T7 eukaryotic plasmid were co-transfected into BHK-21 cells for virus rescue.The experimentalresults indicated that the Japanese encephalitis virus could be successfully res-cuedfrom BHK-21 cells.The plaque experiment and mouse experiment indicated that the rescued virus had similar replication ability and pathogenicity with wild type virus.It was confirmed that the infectious clone of genotype Ⅰ Japanese encephalitis virus GX strain was successfully construc-ted in this study.

The infectious clone plasmid of genotype Ⅰ Japanese encephalitis virus GX strain was suc-cessfully obtained by reverse transcription-polymerase chain reaction,where the cDNA of GX strain is divided into three fragments for amplification and the three fragments were sequentially cloned into pBR322 vector.After the infectious clone plasmid pGX was sequenced correctly,the pGX and pCAGGS-T7 eukaryotic plasmid were co-transfected into BHK-21 cells for virus rescue.The experimentalresults indicated that the Japanese encephalitis virus could be successfully res-cuedfrom BHK-21 cells.The plaque experiment and mouse experiment indicated that the rescued virus had similar replication ability and pathogenicity with wild type virus.It was confirmed that the infectious clone of genotype Ⅰ Japanese encephalitis virus GX strain was successfully construc-ted in this study.