Objective:To compare and analyze the effects of time-restricted feeding (TRF) and exercise intervention on postoperative outcomes in obese children with concealed penis following corrective surgery, and to identify more effective postoperative management strategies.Methods:A retrospective study was conducted on the clinical data of obese boys aged 6-12 years who underwent modified Devine surgery for concealed penis in the Department of Pediatric Surgery, the Second Affiliated Hospital of Shandong First Medical University, between October 1, 2022, and June 30, 2024. According to the postoperative weight management intervention, patients were divided into three groups: an exercise group, a TRF group, and a non-intervention group. The exercise and TRF groups received 6 months of structured exercise or TRF-based weight management, respectively. During TRF intervention, all caloric intake was confined to a 10-hour period each day, with no energy intake during the remaining 14 hours. Postoperative follow-up was conducted daily for 6 months. The preoperative suprapubic fat thickness (h1) and penile length (d1), immediate postoperative penile length (d2), and 6-month postoperative suprapubic fat thickness (h2) and penile length (d3) were measured. At 6 months after surgery, changes in body mass index (BMI), penile exposure increment (Δd = d3-d1), and suprapubic fat thickness were compared among the three groups. Statistical analyses were performed using SPSS version 27.0.1. Data conforming to a normal distribution were expressed as Mean ± SD. One-way analysis of variance (ANOVA) or Welch’s ANOVA was used for intergroup comparisons, followed by independent sample t-test and Bonferroni correction or Games-Howell tests for post hoc multiple comparisons when significant differences were identified. A P value < 0.05 was considered statistically significant. Results:A total of 101 male patients were enrolled: 35 in the exercise group, 31 in the TRF group, and 35 in the non-intervention group. No significant differences were observed among the three groups in age, preoperative BMI, h1, d1, or d2 (all P > 0.05), indicating comparable baseline characteristics. At 6 months postoperatively, the BMI of the TRF group was significantly lower than that of the exercise and non-intervention groups [(20.82±1.19) kg/m 2 vs. (22.46±2.00) kg/m 2 vs. (23.52±2.52) kg/m 2,P < 0.05]; the BMI in the exercise group showed a decreasing trend compared with the non-intervention group, although the difference was not statistically significant ( P>0.05); the penile exposure increment (Δd) was greater in the TRF group than in the exercise and non-intervention groups [(2.08±0.43) cm vs. (1.68 ± 0.37) cm vs. (1.47 ± 0.16) cm], while the suprapubic fat thickness (h2) was smaller [(8.65±1.96) mm vs. (10.43±1.32) mm vs. (11.26±1.53) mm]; the exercise group also demonstrated a larger Δd and a smaller h2 than the non-intervention group, and these differences were statistically significant (all P<0.05). Conclusion:Both TRF and regular exercise are effective postoperative weight management interventions for maintaining surgical outcomes in obese children with concealed penis after modified Devine surgery. Among these approaches, TRF demonstrates superior efficacy.

AIM:To investigate the preventive and therapeutic effects of sesamin(SES)on fatty liver disease in rats with hypertension combined with dyslipidemia,and to explore the potential mecha-nisms based on mRNA-seq.METHODS:Spontane-ously hypertensive rats(SHRs)were fed a high-fat,high-cholesterol diet to establish a rat model of hy-pertension combined with dyslipidemia,and then treated with SES for 16 weeks continuously.The ex-periment was divided into four groups:WKY,SHR,Model,and Model+SES(160 mg·kg-1·d-1).Blood pressure was measured using the tail-cuff method.Body weight was monitored,and body mass index was calculated.Liver morphology was detected by ultrasound,and liver thickness was measured.Liver wet weight was weighed,and liver index was calcu-lated.Liver volume was detected by the water dis-placement method.Serum triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),alanine aminotransferase(ALT),aspartate amino-transferase(AST),and total bile acids(TBA)were de-tected by ELISA.Liver sequencing analysis was per-formed using mRNA-seq.Liver histomorphological changes were observed by HE staining.The degree of hepatic steatosis was observed by Oil Red O stain-ing,and the degree of hepatic fibrosis was observed by MASSON staining.The mRNA expression of Al-dh1a7,Nnmt,Irs2,Pltp,and Scd was detected by q-PCR.The protein expression of Scd,Nnmt,AMPK,p-AMPK,PPARα,and PPARγ was detected by Western blotting.RESULTS:After 16 weeks of continuous SES administration to rats with hypertension combined with dyslipidemia,blood pressure was significantly reduced(P＜0.01),and body weight was decreased.Serum TG,TC,and LDL-C levels were decreased,while HDL-C levels were increased.Serum ALT and AST levels were decreased.Liver weight,organ in-dex,liver thickness,and liver volume were de-creased.The degree of hepatic steatosis and hepat-ic fibrosis was improved.A total of 545 differentially expressed mRNAs were identified in the livers of rats in each group,of which 278 were upregulated and 267 were downregulated.Among the 27 com-monly differentially expressed mRNAs,five mRNAs related to lipid metabolism were screened,namely Aldh1a7,Nnmt,Irs2,Pltp,and Scd.KEGG enrich-ment analysis showed that the enriched pathways were AMPK and PPAR.Further validation revealed that in the SES-treated group,the mRNA expression of Scd in the liver was decreased,while the mRNA expression of Nnmt was increased.The protein ex-pression of Scd was decreased,while the protein ex-pression of Nnmt,AMPK,p-AMPK,PPARα,and PPARγ was increased.CONCLUSION:SES has preven-tive and therapeutic effects on fatty liver disease in rats with hypertension combined with dyslipidemia,and its mechanism of action may be related to the reduction of Scd expression levels in the liver and the increase in the expression of Nnmt,AMPK,p-AMPK,PPARα,and PPARγ.

AIM:To investigate the preventive and therapeutic effects of sesamin(SES)on fatty liver disease in rats with hypertension combined with dyslipidemia,and to explore the potential mecha-nisms based on mRNA-seq.METHODS:Spontane-ously hypertensive rats(SHRs)were fed a high-fat,high-cholesterol diet to establish a rat model of hy-pertension combined with dyslipidemia,and then treated with SES for 16 weeks continuously.The ex-periment was divided into four groups:WKY,SHR,Model,and Model+SES(160 mg·kg-1·d-1).Blood pressure was measured using the tail-cuff method.Body weight was monitored,and body mass index was calculated.Liver morphology was detected by ultrasound,and liver thickness was measured.Liver wet weight was weighed,and liver index was calcu-lated.Liver volume was detected by the water dis-placement method.Serum triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),alanine aminotransferase(ALT),aspartate amino-transferase(AST),and total bile acids(TBA)were de-tected by ELISA.Liver sequencing analysis was per-formed using mRNA-seq.Liver histomorphological changes were observed by HE staining.The degree of hepatic steatosis was observed by Oil Red O stain-ing,and the degree of hepatic fibrosis was observed by MASSON staining.The mRNA expression of Al-dh1a7,Nnmt,Irs2,Pltp,and Scd was detected by q-PCR.The protein expression of Scd,Nnmt,AMPK,p-AMPK,PPARα,and PPARγ was detected by Western blotting.RESULTS:After 16 weeks of continuous SES administration to rats with hypertension combined with dyslipidemia,blood pressure was significantly reduced(P＜0.01),and body weight was decreased.Serum TG,TC,and LDL-C levels were decreased,while HDL-C levels were increased.Serum ALT and AST levels were decreased.Liver weight,organ in-dex,liver thickness,and liver volume were de-creased.The degree of hepatic steatosis and hepat-ic fibrosis was improved.A total of 545 differentially expressed mRNAs were identified in the livers of rats in each group,of which 278 were upregulated and 267 were downregulated.Among the 27 com-monly differentially expressed mRNAs,five mRNAs related to lipid metabolism were screened,namely Aldh1a7,Nnmt,Irs2,Pltp,and Scd.KEGG enrich-ment analysis showed that the enriched pathways were AMPK and PPAR.Further validation revealed that in the SES-treated group,the mRNA expression of Scd in the liver was decreased,while the mRNA expression of Nnmt was increased.The protein ex-pression of Scd was decreased,while the protein ex-pression of Nnmt,AMPK,p-AMPK,PPARα,and PPARγ was increased.CONCLUSION:SES has preven-tive and therapeutic effects on fatty liver disease in rats with hypertension combined with dyslipidemia,and its mechanism of action may be related to the reduction of Scd expression levels in the liver and the increase in the expression of Nnmt,AMPK,p-AMPK,PPARα,and PPARγ.

Objective:To compare and analyze the effects of time-restricted feeding (TRF) and exercise intervention on postoperative outcomes in obese children with concealed penis following corrective surgery, and to identify more effective postoperative management strategies.Methods:A retrospective study was conducted on the clinical data of obese boys aged 6-12 years who underwent modified Devine surgery for concealed penis in the Department of Pediatric Surgery, the Second Affiliated Hospital of Shandong First Medical University, between October 1, 2022, and June 30, 2024. According to the postoperative weight management intervention, patients were divided into three groups: an exercise group, a TRF group, and a non-intervention group. The exercise and TRF groups received 6 months of structured exercise or TRF-based weight management, respectively. During TRF intervention, all caloric intake was confined to a 10-hour period each day, with no energy intake during the remaining 14 hours. Postoperative follow-up was conducted daily for 6 months. The preoperative suprapubic fat thickness (h1) and penile length (d1), immediate postoperative penile length (d2), and 6-month postoperative suprapubic fat thickness (h2) and penile length (d3) were measured. At 6 months after surgery, changes in body mass index (BMI), penile exposure increment (Δd = d3-d1), and suprapubic fat thickness were compared among the three groups. Statistical analyses were performed using SPSS version 27.0.1. Data conforming to a normal distribution were expressed as Mean ± SD. One-way analysis of variance (ANOVA) or Welch’s ANOVA was used for intergroup comparisons, followed by independent sample t-test and Bonferroni correction or Games-Howell tests for post hoc multiple comparisons when significant differences were identified. A P value < 0.05 was considered statistically significant. Results:A total of 101 male patients were enrolled: 35 in the exercise group, 31 in the TRF group, and 35 in the non-intervention group. No significant differences were observed among the three groups in age, preoperative BMI, h1, d1, or d2 (all P > 0.05), indicating comparable baseline characteristics. At 6 months postoperatively, the BMI of the TRF group was significantly lower than that of the exercise and non-intervention groups [(20.82±1.19) kg/m 2 vs. (22.46±2.00) kg/m 2 vs. (23.52±2.52) kg/m 2,P < 0.05]; the BMI in the exercise group showed a decreasing trend compared with the non-intervention group, although the difference was not statistically significant ( P>0.05); the penile exposure increment (Δd) was greater in the TRF group than in the exercise and non-intervention groups [(2.08±0.43) cm vs. (1.68 ± 0.37) cm vs. (1.47 ± 0.16) cm], while the suprapubic fat thickness (h2) was smaller [(8.65±1.96) mm vs. (10.43±1.32) mm vs. (11.26±1.53) mm]; the exercise group also demonstrated a larger Δd and a smaller h2 than the non-intervention group, and these differences were statistically significant (all P<0.05). Conclusion:Both TRF and regular exercise are effective postoperative weight management interventions for maintaining surgical outcomes in obese children with concealed penis after modified Devine surgery. Among these approaches, TRF demonstrates superior efficacy.

【Objective】 To investigate the relationship between anti-platelet antibodies, therapeutic effect of intravenous immunoglobulin (IVIG) and Treg/Th17 cells imbalance in children with immune thrombocytopenia (ITP). 【Methods】 The changes and correlation of platelet count and Treg/Th17 ratio before and after IVIG treatment in 60 newly diagnosed ITP children with anti-platelet antibodies and 60 children with primary ITP without anti-platelet antibodies were analyzed. 【Results】 1) Compared with the control group, the efficacy of IVIG treatment was better in children with ITP in the case group(CR+ R cases: 50 vs 32) (P<0.01). 2) After IVIG treatment, platelet count(×10⁹/L)(case: 4.5±2.9 vs 327.4±69.5, control: 4.1±3.2 vs 304.7±75.9), Treg cell level(%)(case: 2.15±1.08 vs 5.09±1.37, control: 2.41±0.92 vs 4.98±1.10), Treg/Th17 ratio(case: 1.10±0.19 vs 7.75±1.11, control: 1.27±0.21 vs 4.69±0.81)significantly increased while Th17 cell level(%) significantly decreased in the 2 groups of children(case: 2.07±1.31 vs 1.37±0.92, control: 2.13±1.18 vs 1.48±1.01); compared with the control group, there was no significant change in Treg, Th17 and Treg/Th17 ratio before and after treatment in the case group (P>0.05), but platelet count increased more significantly (P<0.05). 3) There were 3 positive cases in the control group and 12 negative cases in the case group after IVIG treatment, and IVIG treatment probably had no effect on the positive rate of anti-platelet antibodies in children with ITP (P>0.05). 4) The change in platelet count after IVIG treatment was significantly positively correlated with Treg levels (r=0.49 in the case group and r=0.441 in the control group) and negatively correlated with Th17 cell levels (r=-0.390 in the case group and r=-0.364 in the control group). 【Conclusion】 Anti-platelet antibodies can be used as a predictor of the efficacy of IVIG therapy in children with ITP, but they are not associated with changes in the Treg/Th17 ratio.

Objective To establish a reliable pretreatment method for the detection of mequindox and its metabolite in pork luncheon meat by ultra-performance liquid chromatography/triple qudrupole tandem mass spectrometry. Methods Samples were extracted with ethyl acetate, and the results of purification and enrichment by PAX and PEP solid-phase extraction columns were analyzed. Acetonitrile/methanol (3:11) - 0.1% formic acid water was used as the mobile phase, and Shimadzu Inertsil ODS-3-column (3µm, 2.1 × 100mm) chromatographic columns were used for qualitative and quantitative analysis using the multi-reaction detection positive ion mode. Results The results showed that PEP cartridge had good recovery rate. The detection limit of mequindox was 0.10µg/kg, and limit of quantitation was 0.30µg/kg. The average recoveries for spiked levels of 0.33, 0.83, and 1.65µg/kg were 127%, 72.0%, and 60.1%, respectively. The detection limit of 2-quinoxalinecarboxylic acid was 0.10µg/kg, and limit of quantitation was 0.40µg/kg. The average recoveries for spiked levels of 0.42, 1.05, and 2.1µg/kg were 125%, 99.0%, and 60.9%, respectively. Conclusion This method is suitable for the determination of mequindox and its metabolite 2-quinoxalinecarboxylic acid in luncheon meat.

Objective To develop a scale of assessing inpatient′s risk factors of chemotherapy drugs permeability. Methods Designed a questionnaire for expert information collecting based on literature review,and employed the Delphi method to develop a set of indicators to measure inpatient′s effective store seepage risk factors. Fifteen experts finished the 2-round survey. Results Expert's enthusiasm was high, the two rounds of enquiry for effective questionnaire recovery rate was 100%. Expert's authority was very high, the average duration of clinical practice was 23.8 years, the average duration of nursing management was 13.1 years, the rate of familiarity about this kind of research was 100%, the recognition degree in the accumulation of theoretical knowledge, the clinical practice and the home or abroad research degree was 93%, 100%and 73%respectively. Two rounds of enquiry for expert advice of Cronbach's alpha coefficient was 0.96 and 0.98 respectively, experts tend to agree that the scale had good consistency, high credibility, delivery system eventually formed contains, vein, the static drop time (static) chemotherapy, more routine chemotherapy, liquid type, drug factors, social support, cooperation degree, liquid volume, mission 11 dimensions such as level of understanding, the nurse puncture store effective seepage risk factors assessment of 42 items. Conclusion The inpatient′s effective store seepage risk factor assessment scale developed by using the Delphi method is reliable for assessing effective store seepage risk factor of hospitalized patients .

OBJECTIVE: To investigate the effect of cerebral hemodynamic changes on white matter damage in premature infant with patent ductus arteriosus(PDA). METHODS: A total of 106 premature infants were enrolled in the study, including 35 PDA infants with hemodynamic changes (hsPDA group), 35 PDA infants without hemodynamic changes (non-hsPDA group) and 36 non-PDA infants (control group). Serum level of neuron-specific enolase (NES) was detected and craniocerebral ultrasound examination was performed on d3, d7 and d14 after birth. The correlation between blood flow rate of PDA and gray scale value of lateral ventricle was analyzed. RESULTS: Gray scale values of lateral ventricle and serum levels of NES in hsPDA group were higher than those in control group on d3, d7 and d14 (P<0.01), but no significant difference was observed between non-hsPDA group and control group (P>0.05). There was a positive correlation between the blood flow rate of PDA and gray scale value of lateral ventricle (r=0.876, P<0.01) in premature infants. CONCLUSION Patent ductus arteriosus with hemodynamic changes is closely related to white matter damage in premature infants, and early intervention is necessary.
Ductus Arteriosus, Patent ; complications ; Hemodynamics ; Humans ; Infant ; Infant, Newborn ; Infant, Premature ; Ultrasonography ; White Matter ; pathology

Aged ; Aged, 80 and over ; Humans ; Male ; Porokeratosis ; genetics ; Rectal Neoplasms ; genetics