Hypoxemia is a common pathological state characterized by low oxygen saturation in the blood. This condition compromises mucosal barrier integrity particularly in the gut and oral cavity. However, the mechanisms underlying this association remain unclear. This study used periodontitis as a model to investigate the role of platelet activation in oral mucosal immunopathology under hypoxic conditions. Hypoxia upregulated methyltransferase-like protein 4 (METTL4) expression in platelets, resulting in N⁶-methyladenine modification of mitochondrial DNA (mtDNA). This modification impaired mitochondrial transcriptional factor A-dependent cytosolic mtDNA degradation, leading to cytosolic mtDNA accumulation. Excess cytosolic mt-DNA aberrantly activated the cGAS-STING pathway in platelets. This resulted in excessive platelet activation and neutrophil extracellular trap formation that ultimately exacerbated periodontitis. Targeting platelet METTL4 and its downstream pathways offers a potential strategy for managing oral mucosa immunopathology. Further research is needed to examine its broader implications for mucosal inflammation under hypoxic conditions.
DNA, Mitochondrial/metabolism* ; Mouth Mucosa/pathology* ; Hypoxia/immunology* ; Methyltransferases/metabolism* ; Blood Platelets/metabolism* ; Animals ; Periodontitis/immunology* ; Humans ; Platelet Activation ; Mice

Objective To cope with the severe situation of stroke prevention and control,and to solve the problems of lack of awareness of early prevention and treatment of diseases,difficulty in seeking medical treatment,loose structure of medical consortia,and low efficiency of medical resource utilization among the masses in China,fully mobilize resources from all parties,and jointly achieve the goal of system construction.Methods Taking the establishment of a stroke medical service system led by a large tertiary hospital as an example,a theoretical analysis model of the actor network of stroke prevention and control medical consortia was constructed.The translation principle was used to conduct in-depth analysis of heterogeneous actors,mandatory ac-cess points,recruitment,benefit sharing,and mobilization in the stroke prevention and control medical consortium network,bal-ance the interests and demands of all parties,and eliminate obstacles in the operation system.Results By applying the actor network theory method,consolidating multidisciplinary forces within the hospital,strengthening quality control,enhancing region-al collaborative treatment,conducting screening,education,and training to promote early screening,diagnosis,and treatment,the number of patients treated in the stroke center showed an increasing trend,with an average annual growth rate of 10.93％.The treatment time of the stroke center was significantly shortened.In the past four years,the median DNT time was shortened by 15 minutes,and the median DPT time was shortened by 67 minutes.Conclusion Multiple departments,institutions,and disci-plines can collaborate to strengthen patient education,conduct screening,enhance information technology construction and quality control,mobilize various stakeholders in the system,and strive to properly solve the problem of stroke prevention and control.

Objective To cope with the severe situation of stroke prevention and control,and to solve the problems of lack of awareness of early prevention and treatment of diseases,difficulty in seeking medical treatment,loose structure of medical consortia,and low efficiency of medical resource utilization among the masses in China,fully mobilize resources from all parties,and jointly achieve the goal of system construction.Methods Taking the establishment of a stroke medical service system led by a large tertiary hospital as an example,a theoretical analysis model of the actor network of stroke prevention and control medical consortia was constructed.The translation principle was used to conduct in-depth analysis of heterogeneous actors,mandatory ac-cess points,recruitment,benefit sharing,and mobilization in the stroke prevention and control medical consortium network,bal-ance the interests and demands of all parties,and eliminate obstacles in the operation system.Results By applying the actor network theory method,consolidating multidisciplinary forces within the hospital,strengthening quality control,enhancing region-al collaborative treatment,conducting screening,education,and training to promote early screening,diagnosis,and treatment,the number of patients treated in the stroke center showed an increasing trend,with an average annual growth rate of 10.93％.The treatment time of the stroke center was significantly shortened.In the past four years,the median DNT time was shortened by 15 minutes,and the median DPT time was shortened by 67 minutes.Conclusion Multiple departments,institutions,and disci-plines can collaborate to strengthen patient education,conduct screening,enhance information technology construction and quality control,mobilize various stakeholders in the system,and strive to properly solve the problem of stroke prevention and control.

Objective:To investigate the correlation between antiplatelet agents and the risk of ruptured intracranial aneurysm.Methods:Patients with intracranial aneurysm admitted to the Department of Neurology, East Hospital Area of Qingdao Municipal Hospital from June to December 2018 were selected retrospectively. The baseline data of patients and the characteristics of intracranial aneurysms were collected. The independent correlation between antiplatelet agents and the risk of ruptured intracranial aneurysm was identified by the univariable analysis and multivariate logistic regression analysis. Results:A total of 90 patients with intracranial aneurysm were included in the study. There were 31 males (34.44%) and 59 females (65.56%). The median diameter of the aneurysm was 4 mm. Forty-six patients taking antiplatelet agents before being diagnosed with intracranial aneurysm, of which 36 taking aspirin, 3 taking clopidogrel, and 7 taking aspirin+ clopidogrel. There were 31 patients (34.44%) with ruptured aneurysm and 59 (65.56%) with unruptured aneurysm. There were statistical differences in the proportion of patients with age <60 years ( P<0.05), diabetes ( P<0.1), ischemic heart disease ( P<0.05), history of previous stroke or transient ischemic attack ( P<0.01), internal carotid artery aneurysm ( P<0.01), anterior communicating artery aneurysm ( P<0.05), posterior communicating artery aneurysm ( P<0.01) and taking antiplatelet agents before diagnosis ( P<0.1) between the ruptured group and the unruptured group. Multivariate logistic regression analysis showed that age <60 years (odds ratio[ OR] 4.116, 95% confidence interval [ CI] 1.337-12.673; P=0.014), anterior communicating artery aneurysm ( OR 5.015, 95% CI 1.155-22.559; P=0.032) and posterior communicating artery aneurysm ( OR 68.796, 95% CI 6.762-699.951; P<0.001) were the independent risk factors for ruptured intracranial aneurysm, and taking antiplatelet agents was an independent protective factor for ruptured intracranial aneurysm ( OR 0.320, 95% CI 0.104-0.992; P=0.048). Conclusions:Taking antiplatelet agents, especially aspirin, does not increase the risk of ruptured intracranial aneurysm, but may be a protective factor of ruptured intracranial aneurysm. Unruptured aneurysms are not contraindications for antiplatelet therapy in patients with clear indications.