Based on the theory of "disease of both blood and water" in Essentials from the Golden Cabinet （《金匮要略》）， and in combination with the dynamic syndrome evolution of heart failure with preserved ejection fraction （HFpEF）， this paper systematically clarifies the pathomechanism of HFpEF， characterized by yang deficiency as the root， blood stasis as the pivotal factor and water retention as the manifestation. Accordingly， the therapeutic principles have been proposed， which are warming yang and banking up original qi to consolidate the root， activating blood and unblocking collaterals to smooth the mechanism， and promoting urination and regulating pivot to remove the branch. On this basis， a compound formula structure of "one monarch， one minister and one assistant" is established， forming an integrated intervention strategy that synergistically combines the three methods of warming yang， activating blood， and promoting urination through combined classical formulas. Zhenwu Decoction （真武汤）， which warms yang and dissolves rheum， is used to consolidate the root and directly target the source of yang deficiency， serving as the monarch； Guizhi Fuling Pills （桂枝茯苓丸）， which activates blood， promotes urination and unblocks the pivot， assists in interrupting the binding of blood stasis and water retention， serving as the minister； Tingli Dazao Xiefei Decoction （葶苈大枣泻肺汤）， which regulates qi， disperses retained fluids， and eliminates the manifestation， alleviates acute water-retention symptoms， serving as the assistant. This compound formula is warming without being drying， diuretic without being drastic， and dispels stasis without consuming blood， thereby achieving the therapeutic effects of warming yang， activating blood， and promoting urination.

Lung cancer is a leading cause of cancer-related morbidity and mortality worldwide. Coupled with the substantial workload, the clinical management of lung cancer is challenged by the critical need to efficiently and accurately process increasingly complex medical information. In recent years, large language models (LLMs) technology has undergone explosive development, demonstrating unique advantages in handling complex medical data by leveraging its powerful natural language processing capabilities, and its application value in the field of lung cancer diagnosis and treatment is continuously increasing. The paper systematically analyzes that the exceptional potential of LLMs in lung cancer auxiliary diagnosis, tumor feature extraction, automatic staging, progression/outcome analysis, treatment recommendations, medical documentation generation, and patient education. However, they face critical technical and ethical challenges including inconsistent performance in complex integrated decision-making (e.g., TNM staging, personalized treatment suggestions) and "black box" opacity issues, along with dilemmas such as training data biases, model hallucinations, data privacy concerns, and cross-lingual adaptation challenges ("data colonization"). Future directions should prioritize constructing high-quality multimodal corpora specific to lung cancer, developing interpretable and compliant specialized models, and achieving seamless integration with existing clinical workflows. Through dual drivers of technological innovation and ethical standardization, LLMs should be prudently advanced for holistic lung cancer management processes, ultimately promoting efficient, standardized, and personalized diagnosis and treatment practices.

The Dunhuang manuscript Fuxing Jue takes the "Tangye Jingfa Tu" as the core of its theory on prescription and compatibility. Its medication principles mainly include the "five elements principle of tonifying and purging" and the "five elements principle of elimination and transformation". Based on the differentiation of deficiency and excess in the five Zang organs， it flexibly applies medicinal properties， usage， and flavor transformation for tonifying and purging， forming its unique method of medication and compatibility. In Taiyin disease， "fullness syndrome" often occurs together with "diarrhea"， and these two conditions also serve as the primary indications for the middle-regulating formulas. Among them， Lizhong Wan （Tang） mainly address Taiyin deficiency. The three Xiexin Tang （Banxia Xiexin Tang， Gancao Xiexin Tang， Shengjiang Xiexin Tang） address Taiyin deficiency accompanied by pathogenic excess. The Sini Tangand Tongmai Sini Tang primarily treat dysfunction of the liver， spleen， and kidney with impaired opening and closing of Taiyin， manifesting as diarrhea. The medicinal flavors of middle-regulating formulas are pungent， sweet， and bitter， acting directly on the spleen of Taiyin. The pungent flavor induces purging of the spleen， sweet flavor tonifies the spleen， and bitter flavor eliminates lumps. When the constituent medicinal units of middle-regulating formulas are unified， the ratio of pungent to sweet flavors reflects the tonic and purgative strength of the formula. In addition， the two decoction methods， "short-term decoction to extract Qi" and "long-term decoction to extract flavor"， also influence the formula's tonifying and purgative effects. Based on the composition of flavors and special decoction methods， this article discusses the differences in the use of middle-regulating formulas for treating "“fullness syndrome" versus "diarrhea". Dysfunction of the spleen can give rise to various diseases. Therefore， middle-regulating formulas are not limited to treating "deficiency， cold， and dampness" syndromes. Later generations of physicians further modified Lizhong Tang to treat "excess， heat， and dryness" syndromes， laying a solid foundation for more flexible and effective clinical application of middle-regulating formulas.

The Dunhuang manuscript Fuxing Jue takes the "Tangye Jingfa Tu" as the core of its theory on prescription and compatibility. Its medication principles mainly include the "five elements principle of tonifying and purging" and the "five elements principle of elimination and transformation". Based on the differentiation of deficiency and excess in the five Zang organs， it flexibly applies medicinal properties， usage， and flavor transformation for tonifying and purging， forming its unique method of medication and compatibility. In Taiyin disease， "fullness syndrome" often occurs together with "diarrhea"， and these two conditions also serve as the primary indications for the middle-regulating formulas. Among them， Lizhong Wan （Tang） mainly address Taiyin deficiency. The three Xiexin Tang （Banxia Xiexin Tang， Gancao Xiexin Tang， Shengjiang Xiexin Tang） address Taiyin deficiency accompanied by pathogenic excess. The Sini Tangand Tongmai Sini Tang primarily treat dysfunction of the liver， spleen， and kidney with impaired opening and closing of Taiyin， manifesting as diarrhea. The medicinal flavors of middle-regulating formulas are pungent， sweet， and bitter， acting directly on the spleen of Taiyin. The pungent flavor induces purging of the spleen， sweet flavor tonifies the spleen， and bitter flavor eliminates lumps. When the constituent medicinal units of middle-regulating formulas are unified， the ratio of pungent to sweet flavors reflects the tonic and purgative strength of the formula. In addition， the two decoction methods， "short-term decoction to extract Qi" and "long-term decoction to extract flavor"， also influence the formula's tonifying and purgative effects. Based on the composition of flavors and special decoction methods， this article discusses the differences in the use of middle-regulating formulas for treating "“fullness syndrome" versus "diarrhea". Dysfunction of the spleen can give rise to various diseases. Therefore， middle-regulating formulas are not limited to treating "deficiency， cold， and dampness" syndromes. Later generations of physicians further modified Lizhong Tang to treat "excess， heat， and dryness" syndromes， laying a solid foundation for more flexible and effective clinical application of middle-regulating formulas.

Objective: To investigate whether insufficient nutritional health knowledge can increase the risk of obesity conferred by the surrounding environment, so as to provide a basis for improving the physical health of primary school students by their knowledge of nutritional health. Methods: From May to June 2017, a stratified cluster random sampling method was used to select 3 669 primary school students from 2 primary schools in Shenyang for anthropometric measurements and a student questionnaire survey. School neighborhood environment data were obtained from geospatial analyses conducted by using SuperMap GIS 9D. A self designed questionnaire based on the Chinese Dietary Guidelines (2016) was used to assess individual nutritional health knowledge levels.The frequency of fruit and vegetable intake was divided into four quartiles ( Q 1- Q 4), and the land use efficiency was divided into three quintiles ( T 1- T 3). Multiple Logistic regression and linear regression analyses were employed to examine the relationships between insufficient nutritional health knowledge, school neighborhood environment, and the frequency of fruit and vegetable intake, body mass index (BMI), and perirenal fat thickness (PrFT) among primary school students. Results: After adjusting for age, sex, place of residence, family annual income, parental education level, and physical activity, multiple Logistic regression analysis showed that higher land use efficiency around school ( T 2 and T 3)( OR =1.37, 2.45), the presence of ≥1 shopping mall ( OR =1.43), and ≥1 fruit and vegetable store ( OR =1.51) were associated with a higher frequency of fruit and vegetable intake ( Q 4) (all P <0.05). Multiple linear regression analysis revealed that students attending schools with higher land use ( T 3: β = -0.72 ), ≥1 shopping mall ( β = -0.45 ), and multiple fruit and vegetable stores ( β =-0.33) had lower PrFT (all P <0.05). Interaction analysis indicated that the positive association between school neighborhood environment and fruit and vegetable intake frequency was attenuated by insufficient nutritional health knowledge [ T 3 land use×insufficient nutritional knowledge score ( OR = 0.05); ≥1 shopping mall × insufficient nutritional knowledge score ( OR =0.06); ≥1 fruit and vegetable store×insufficient nutritional knowledge score( OR =0.07)] (all P < 0.05). The negative association between school neighborhood environment and the extreme quartile of PrFT was attenuated by insufficient nutritional health knowledge [ T 3 land use mix×insufficient nutritional knowledge score ( β =2.31); ≥1 shopping mall×insufficient nutritional knowledge score( β =3.59)](both P <0.05). Conclusions The healthy food environment around schools is negatively correlated with the obesity risk among primary school students.However, the association is affected by their insufficient nutritional knowledge. Comprehensive intervention strategies should be implemented to prevent the prevalence of obesity by addressing both individual and environmental factors influencing dietary habits.

Objective:To construct a management program for postoperative home exercise rehabilitation in patients undergoing liver cancer surgery, and to provide guidance for improving home exercise capacity after liver cancer surgery.Methods:A mixed study design was used to summarize the best evidence for a postoperative home exercise rehabilitation program for patients with liver cancer based on a literature review from January to June 2022, and the first draft of a postoperative home exercise rehabilitation program for liver cancer patients was formed after discussion in the research team, and discussed in an expert validation meeting, and the first draft of the program was revised and improved according to the expert′s opinion in combination with clinical practice.Results:This study included 14 articles, including 4 clinical guidelines, 3 systematic evaluations, 2 randomized controlled studies, 3 class experimental research and 2 expert consensuses. And 22 pieces of evidence were included in the summary of evidence, including the principle of exercise, supervision of exercise, type of exercise, time and frequency of exercise, and intensity of exercise. A total of 10 experts participated in the expert validation meeting, with 6 males and 4 females, aged (53.31 ± 10.23) years, with an expert authority coefficient of 0.944, and the final home exercise rehabilitation program for postoperative patients with liver cancer was formed to include six aspects, including pre-exercise assessment, warm-up exercise, whole-body exercise, stretching exercise, organizing exercise, and exercise detection and follow-up.Conclusions:The home exercise rehabilitation program for postoperative liver cancer surgery patients is scientific, practical and feasible, and it is a systematic, standardized and safe exercise management model, which can be used for home guidance of postoperative exercise rehabilitation for liver cancer patients.

Objective To describe the current status of post-traumatic growth among family caregivers of dementia and analyze its influencing factors to provide a reference for the development of post-traumatic growth interventions.Methods A convergent mixed-methods study was adopted.Family caregivers of older adults with dementia who were outpatients at a tertiary hospital and a mental health center in Shanghai from May to December 2024 were conveniently selected for the study.A general information questionnaire,the Chinese-Posttraumatic Growth Inventory,the Simplified Coping Style Questionnaire,the Simplified Chinese Event Related Rumination Inventory,the Connor-Davidson Resilience Scale,and the Social Support Rating Scale were used for data collection.The survey was conducted,and the data were analyzed using multivariate stepwise linear regression.19 family caregivers of dementia patients were interviewed in a semi-structured manner using purposive sampling,and the interview data were analyzed using directed content analysis.Results The dementia family caregivers' post-traumatic growth score was(64.73±11.43).The regression results showed that patients' dementia type,types of comorbid chronic diseases,knowledge of the disease,deliberate rumination,positive coping styles,psychological resilience,and social support were the influencing factors of post-traumatic growth(P＜0.05).Qualitative results:interviews refined 8 themes based on 3 dimensions,including individual factors,family and social factors,and cultural and environmental factors.The integration results indicate that both quantitative and qualitative findings were validated and complemented.Conclusion Dementia family caregivers' post-traumatic growth is low to moderate,and the influencing factors are associated with 3 aspects,namely patient factors,caregiver factors,and socio-cultural factors.Healthcare professionals should pay attention to caregivers' post-traumatic growth and develop personalized interventions based on influencing factors,enhancing their level of post-traumatic growth.

Objective:To explore the effects of mHealth-based home exercise rehabilitation on lower limb muscle strength and quality of life in liver cancer surgery patients after discharge from the hospital.Method:One hundred cases of hepatocellular carcinoma patients who underwent surgery in two tertiary hospi-tals from June 2022 to August 2023 were selected,including 50 cases in the control group and 50 cases in the intervention group.The patients were instructed to carry out home exercise for 3 weeks respectively.The control group was given a home rehabilitation exercise manual,while the intervention group developed mHealth-based exercise rehabilitation based on this exercise prescription.The muscle strength and quality of life levels of the two groups were compared after the intervention.Result:Patients with mHealth-based exercise rehabilitation had significantly higher lower limb muscle strength than the control group after 3 weeks of intervention(P<0.05).The fatigue and nausea,and vomiting symp-toms in the quality of life domains were significantly improved compared with the control group(P<0.05).Conclusion:Home-based exercise rehabilitation based on mHealth can effectively improve lower limb muscle strength,fatigue,and nausea and vomiting symptoms in patients discharged from the hospital after surgery for hepatocellular carcinoma.It is an effective way to improve the rehabilitation of patients discharged from the hospital for hepatocellular carcinoma.

Acceptance commitment therapy is accepted as a new psychological therapy to provide psychological treatment and rehabilitation for soldiers,to ensure their health level and maintain the combat effectiveness of the army.In this paper,the applications of acceptance commitment therapy in military personnel are reviewed,including improving post-traumatic stress disorders,relieving postoperative or chronic pain,mitigating anxiety and depression,controlling weight,quitting smoking,and controlling alcohol use disorders.Acceptance commitment therapy is highly applicable among soldiers,especially veterans,exceedingly effective among female soldiers with post-traumatic stress disorders and better than other traditional therapies in treating post-traumatic stress disorders combined with substance use disorders or combined with chronic pain.However,traditional therapy is more effective than acceptance commitment therapy in improving sleep.Unfortunately,the number of current studies is small,research is not widely-distributed geographically,and domestic studies are lacking.Multi-center randomized controlled studies with large samples on the basis of foreign studies are needed to verify the applicability of acceptance commitment therapy in Chinese military.

Objective: To construct a Family with sequence similarity 50 member A(FAM50A) gene knockout mouse insulinoma pancreatic β-cell line Beta-TC-6 using CRISPR/Cas9 gene editing technology and to prepare polyclonal antibodies specifically recognizing FAM50A. Methods: Two guide RNAs(sgRNAs) targeting the FAM50A gene were designed,and a recombinant plasmid expressing blue fluorescent protein(BFP) was constructed for gene knockout.The successfully constructed plasmid was transfected into Beta-TC-6 cells,and BFP-positive single cells were isolated for clonal expansion.The expanded monoclonal cell lines were genotyped by Sanger sequencing,and FAM50A protein expression was assessed by Western blot.Purified human recombinant FAM50A protein was used to immunize New Zealand rabbits for the preparation of a polyclonal antibody.The specificity of the prepared antibody was then validated using the successfully established FAM50A knockout cell line. Results: A monoclonal cell line with a successful knockout of the FAM50A gene was identified.Sanger sequencing confirmed base deletions at the target site.Western blot analysis showed a complete absence of FAM50A protein expression in this cell line.The prepared polyclonal antibody successfully recognized endogenous murine FAM50A protein in wild-type Beta-TC-6 cells and in hTERT-RPE1 cells overexpressing human FAM50A-GFP fusion protein,while no signal was detected in the FAM50A knockout cells. Conclusion This study successfully established a FAM50A gene knockout Beta-TC-6 cell model and generated a FAM50A polyclonal antibody,providing powerful tools for future research.