Objective:To investigate the effects and mechanisms of angiotensin-converting enzyme 2 (ACE2) and Captopril (CAP) on mechanical ventilation-induced lung injury (VILI) in mice.Methods:Seventy-two healthy male BALB/c mice were randomly assigned (using a random number table) into six groups ( n=12 per group): normal control (NC) group, VILI group, ACE2 group, VILI+ACE2 group, CAP group, and VILI+CAP group. One hour prior to mechanical ventilation, the ACE2 and VILI+ACE2 groups were intraperitoneally injected with ACE2 at a dose of 0.1 mg/kg, while the CAP and VILI+CAP groups were intraperitoneally injected with CAP at a dose of 2.5 mg/kg. Following mechanical ventilation, serum samples were collected and enzyme-linked immunosorbent assay (ELISA) was used to detect inflammatory factors [platelet activating factor (PAF), endothelin-1 (ET-1), soluble intercellular adhesion molecule-1 (sICAM-1), prostaglandin E2 (PGE2)] and cardiovascular system related indicators [von Willebrand factor (vWF), thrombomodulin (TM), angiotensin (Ang) (1-9), Ang (1-7), prostacyclin I 2 (PGI2)]. Bronchoalveolar lavage fluid (BALF) was gathered, and total protein concentration was determined using BCA method, and sICAM-1 levels were measured by ELISA. Lung tissues were collected and subjected to hematoxylin and eosin staining (HE staining) for the assessment of pathological lung injury and lung injury scoring. Western blot and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were utilized to detect the relative expression levels of ACE2 protein and mRNA, respectively. Statistical analysis was performed using IBM SPSS 20.0 software. Intergroup comparisons were conducted using one-way analysis of variance followed by the least significant difference (LSD) test. Results:No statistically significant differences were observed in the levels of PAF, ET-1, sICAM-1, vWF, TM, Ang(1-9), Ang(1-7), and PGI2 in serum and lung tissues between the ACE2/CAP groups and the NC group (all P>0.05). Compared with the VILI group, the VILI+ACE2 and VILI+CAP groups exhibited significantly decreased serum and lung tissue levels of PAF, ET-1, sICAM-1, and vWF (all P<0.05), while the levels of TM, Ang(1-9), Ang(1-7), and PGI2 were significantly increased (all P<0.05). Pathological lung injury was alleviated, and the lung wet/dry weight ratio was significantly reduced (all P<0.05) in the VILI+ACE2 and VILI+CAP groups. Furthermore, both ACE2 protein and mRNA expression levels were significantly increased in these groups (all P<0.05). Conclusion:Both ACE2 and CAP can inhibit inflammation and protect the cardiovascular system, possibly by promoting the ACE2/Ang(1-9)/Ang(1-7) axis, thereby exerting a protective effect against VILI.

Objective:To investigate the impact of pumpkin polysaccharide(PP)on hypoxia/reoxygenation(H/R)-induced myocardial cell(H9C2)injury by regulating Nrf2/γ-GCS pathway.Methods:HR cell models were established,and the concentration of pumpkin polysaccharide(PP)was determined.H9C2 cells were grouped into Control group,H/R group,pumpkin polysaccharide group(PP group,2 mg/L PP),Nrf2 inhibitor group(ML385 group,2.0 μmol/L ML385),and PP+ML385 group(2.0 mg/L PP+2.0 μmol/L ML385),the apoptosis,mitochondrial membrane potential changes,oxidative stress(LDH,MDA,SOD,ROS),the lev-els of inflammatory factors(TNF-α,IL-6,IL-1β)and the protein expressions of Nrf2 and γ-GCS in H9C2 cells were detected,respec-tively.Results:Compared with Control group,H/R group had changes in cell morphology,the cell injury and mitochondrial membrane potential decreased,the H9C2 cell viability,SOD and protein expression levels of Nrf2 and γ-GCS were significantly decreased,while the apoptosis rate,LDH,MDA,ROS,and levels of TNF-α,IL-6 and IL-1β were significantly increased(P<0.05);compared with H/R group,the cell injury in PP group was reduced,the mitochondrial membrane potential was restored,the H9C2 cell viability,SOD and protein expression levels of Nrf2 and γ-GCS were significantly increased,and the apoptosis rate,LDH,MDA,ROS,and levels of TNF-α,IL-6 and IL-1β were significantly decreased(P<0.05),the effect of ML385 was opposite to that of PP,and ML385 could reverse the antioxidant and anti-inflammatory effects of PP.Conclusion:PP up-regulates the Nrf2/γ-GCS pathway to exert antiox-idant and anti-inflammatory effects,and reduces H/R-induced H9C2 cell injury.

Objective:To investigate Chinese myocarditis burden and trends in 1990 and 2019.Methods:Based on the Global Burden of Disease (GBD) 2019 data, the number of patients, the number of new cases, the number of deaths, the disability-adjusted life years (DALYs), as well as the morbidity, mortality, DALYs rate and their age-standardized rates were used to analyze the trend and the burden of myocarditis in the Chinese population in 1990 and 2019.Results:In 2019, the number of patients, the number of new cases and the number of deaths with myocarditis in China were 234 900, 275 100 and 13 100 respectively, increasing by 85.62%, 47.51% and 50.22% compared with 1990. The age-standardized incidence and mortality were 16.94/100 000 and 0.92/100 000, respectively. Compared with 1990, the age-standardized incidence in 2019 decreased by 6.06%, and the mortality decreased by 16.04% respectively. The age-standardized incidence and mortality of Chinese male patients with myocarditis were higher than that of female. Compared with 1990, the age group with the largest incidence and mortality of myocarditis in China in 2019 all shifted to the elder group. And, DALYs and age-normalized DALYs due to myocarditis in China showed a decreasing trend in 2019, from 458 600 and 42.51/100 000 in 1990 to 341 300 and 25.39/100 000 in 2019, respectively. The rate of DALYs and age-standardized DALYs in male patients was always higher than female.Conclusions:Compared with 1990, the overall burden of myocarditis in China showed a downward trend in 2019, and the burden of myocarditis in male patients was higher than female. More attention should be paid to the burden of myocarditis in Chinese elderly population.

Objective To evaluate the therapeutic effect of gamma knife on advanced liver cancer patients with MRI diffusion-weighted imaging (MRI-DWI).Methods MRI-DWI detection was performed in 100 liver cancer patients 1-2 days before gamma knife therapy,7 days and 30 days after gamma knife therapy.Then,comparisons were made between the dynamic changes of ADC values before and after gamma knife therapy under different b-values.Results Under different b values,significant changes in the average ADC values of foci could be noted,before and after gamma knife therapy,and the average ADC values increased with the increase of the b value(P ＜ 0.05).Following gamma knife therapy,the average ADC values of foci all increased as compared with those before therapy.Thirty days after treatment,the average ADC values of foci were increased as compared with those 7 days after treatment.Conclusion ADC value changes after gamma knife therapy in the treatment of advanced liver cancer were closely related with tumor necrosis,and ADC values could be used for the assessment of the efficacy of gamma knife therapy.

Objective To compare the two methods of 3D contrast-enhanced MRA in the diagnosis of upper extremity disea-ses, and also to explore the advantages of the moving-bed technique .Methods Two groups of 20 patients suspected of having upper extremity arterial diseases underwent detections of the non-moving-bed 3D contrast-enhanced MRA and the moving-bed 3D contrast-en-hanced MRA .Results Clear pictures of culprit lesion in the upper extremities ( arterio-venous malformation , hemangioma ) were dis-played in all the 20 cases, and the pictures of upper extremity blood vessel were clearly shown during the whole process in 8 cases, with clear direction of blood supply .Conclusion As compared with the non-moving-bed 3D contrast-enhanced MRA, the moving-bed 3D contrast-enhanced MRA had the following advantages:better continuity and more comprehensive assessment of the nature of upper ex-tremity blood vessel lesion .With this reason, it was a detection technique worth further clinical extension .

Objective To study the CT Imaging features of renal angiomyolipoma, so as to increase the value in clinical diag-nosis.Methods Analyses were made on 6 confirmed cases of renal angiomyolipomas with CT scan.Results Of the 6 cases, 4 were single and 2 were multiple cases, of which 1 was in the right kidney and 3 were in the left kidney, and 2 were in both kidneys, with a total of 12 foci.There were six foci with a diameter smaller than 2cm, and another 6 foci with a diameter larger than 2cm, and the lar-gest diameter of the focus was 8.3 cm ×7.1cm.Renal parenchyma and or circular, elliptical and irregular shape soft tissue shadows and fatty tissue shadows in renal pelvis were clearly seen from the CT scan.Conclusion CT scan revealed that the fatty tissue in renal tumor could confirm the diagnosis of renal angiomyolipomas.

Objective To reveal the expression and potential mechanisms of TGF?_1 and CTGF mRNA in liver of rats with nonalcoholic fatty hepatic fibrosis induced by highly fat-enriched diet.Methods The model group(10 rats) was fed with highly fat-enriched diet while the normal control group(10 rats) was raised by standard animal feeds for 24 weeks.Hepatic histopathological changes were evaluated,and RT-PCR method was used to assay TGF?_1 and CTGF mRNA expression levels in the liver.Results The analysis indicated that liver inflammation and fibrosis were apparently present in model rats.RT-PCR analysis revealed that the expression of TGF?_1 and CTGF in hepatic tissue were inreased in the model group as compared with the normal control group.Conclusions The rats model for hepatic fibrosis can be established by feeding with highly fat-enriched diet for 24 weeks.The expression of TGF?_1 and CTGF were enhanced in hepatic liver tissue of fatty hepatic fibrosis rats,and these changes might stimulate the fatty fibrosis.