Objective:To explore the mediating effect of parental emotional education between family functioning and emotional-behavioral problems in children with amblyopia.Methods:Convenience sampling was used to select children with amblyopia and their parents as study subjects at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, from October 2023 to October 2024. The study subjects were surveyed using the General Information Questionnaire, Positive Rating Scale of Family General Functioning, Strengths and Difficulties Questionnaire, and Parental Meta-Emotion Philosophy Scale.Results:A total of 164 questionnaires were distributed, and 151 valid questionnaires were returned, with a valid return rate of 92.07%. The emotional-behavioral problem score for the pediatric patients was (13.64±2.63). The family functioning of children with amblyopia could directly influence their emotional-behavioral problems, and could also indirectly influence their emotional-behavioral problems through parental emotional education, the mediating effect value was -0.636, accounting for 34.96% (-0.636/-1.819) of the total effect.Conclusions:The emotional-behavioral problems of children with amblyopia still need further attention. The conclusion of this study will provide theoretical basis and practical recommendations for subsequent nursing staff to carry out family management interventions for children with amblyopia, thereby reducing their emotional-behavioral problems.

Objective:To explore the mediating effect of parental emotional education between family functioning and emotional-behavioral problems in children with amblyopia.Methods:Convenience sampling was used to select children with amblyopia and their parents as study subjects at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, from October 2023 to October 2024. The study subjects were surveyed using the General Information Questionnaire, Positive Rating Scale of Family General Functioning, Strengths and Difficulties Questionnaire, and Parental Meta-Emotion Philosophy Scale.Results:A total of 164 questionnaires were distributed, and 151 valid questionnaires were returned, with a valid return rate of 92.07%. The emotional-behavioral problem score for the pediatric patients was (13.64±2.63). The family functioning of children with amblyopia could directly influence their emotional-behavioral problems, and could also indirectly influence their emotional-behavioral problems through parental emotional education, the mediating effect value was -0.636, accounting for 34.96% (-0.636/-1.819) of the total effect.Conclusions:The emotional-behavioral problems of children with amblyopia still need further attention. The conclusion of this study will provide theoretical basis and practical recommendations for subsequent nursing staff to carry out family management interventions for children with amblyopia, thereby reducing their emotional-behavioral problems.

The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion arise. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
Antigens, Differentiation ; biosynthesis ; Electrophysiological Phenomena ; physiology ; Gene Expression Regulation ; physiology ; Genome-Wide Association Study ; Human Embryonic Stem Cells ; cytology ; metabolism ; Humans ; Induced Pluripotent Stem Cells ; cytology ; metabolism ; Multigene Family ; physiology ; Neurons ; cytology ; metabolism ; Transcriptome ; physiology