Background/Aims: Cholangiocarcinoma (CCA) is a primary malignant neoplasm with an extremely poor prognosis. While combined chemoradiotherapy has been demonstrated to delay CCA progression to a certain extent, the absence of specific molecular biomarkers or targets significantly hinders the diagnosis and treatment of CCA. Methods: Through cross-analysis of proteomics and ADMA modificationomics, we identified DDX1 overexpressed in CCA with elevated R602-ADMA modifications. HPLC-MS/MS identified PRMT1 as the methyltransferase and USP10 as the deubiquitinating enzyme for DDX1. Immunofluorescence and nuclear-cytoplasmic partitioning experiments confirmed DDX1’s nuclear localization. GO and KEGG analyses clarify the biological functions of DDX1 in response to hypoxia. RNA-seq transcriptomics analyzed key pathways influenced by DDX1. A hydrodynamic in situ CCA mouse model was established to validate the chemopreventive effects of the PRMT1-specific inhibitor GSK715 on CCA development. Results: DDX1 promotes CCA progression both in vivo and in vitro and can be inhibited by GSK715. Mechanistically, PRMT1 mediates ADMA modification at position R602 of DDX1. This modification promotes DDX1 nuclear localization by recruiting USP10 to deubiquitinate DDX1, while simultaneously inhibiting PRMT1 degradation. DDX1 promotes the transcription of PRMT1 and USP10 by binding to the mRNA 3’UTR region, establishing a positive feedback regulatory pathway. This mechanism promotes the occurrence and development of CCA and can serve as a target for the inhibitor GSK715 to suppress CCA progression. Conclusions Our study identified DDX1-R602-ADMA modification as a novel ADMA modification in CCA. It further confirmed its pivotal role in CCA progression. Targeting the USP10-PRMT1-DDX1 axis may represent a significant therapeutic approach for CCA.

Helicobacter pylori (Hp) is a unipolar, microaerobic, multiflagellar, spiral-shaped Gram-negative bacterium that survives and colonizes human gastric mucosa. As a classⅠcarcinogen associated with gastric cancer, long-term stimulation of gastric mucosa by Hp can cause atrophic gastritis, peptic ulcer, gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. It has been reported that Hp can cause epithelial-mesenchymal transition (EMT) in gastric epithelial cells, thereby inducing gastric cancer. We review the mechanism of Hp-induced EMT in gastric epithelial cells, in order to provide new insights for early diagnosis and targeted therapy of gastric cancer.

Objective:To explore the relationship between the location of puncture points and the occurrence of complications in X-ray assisted percutaneous fluoroscopic gastrostomy (PFG).Methods:The retrospective and descriptive study was conducted. The clinical data of a total of 67 patients, including the gender, age, etiology, nutritional status. All data of 67 patients who received with X-ray assisted PFG surgery during the period from January 2021 to January 2023 in Xuanwu Hospital of Capital Medical University were retrospectively analyzed. There were 42 males and 25 females, aged (57.3±12.6) years, ranging from 22 to 90 years old. The technical success rate, distribution of puncture points, and incidence of complications were described. The relationship between different gastric types and puncture sites and complications was analyzed. Measurement data with normal distribution were represented as mean±standard deviation ( ± s). Count data were represented as numbes and Pearson chi-square test was used between groups. Results:A total of 67 patients with dysphagia were included in our study, all of whom underwent X-ray assisted PFG in our institution. The technical success rate was 100%. In the empty state, the puncture point of 30 patients was located at the midpoint of the gastric cavity, 28 cases were leaned towards to the greater curvature, and 9 cases were leaned towards to the lesser curvature. No operation-related severe complications occurred, such as acute gastrointestinal bleeding and perforation. A total of 7 patients experienced varied degree of pain complication during follow-up period, including 5 cases of waterfall type stomach, which showed significant differences with other gastric types ( χ2=3.889, P=0.049). Pain complication of 6 patients was related to the location of the puncture point, with 5 cases leaning towards to the greater curvature and 1 case leaning towards to the lesser curvature. Conclusions:PFG surgery is safe and reliable, the gastric wall puncture point is not completely consistent between the empty and dilated gastric state. The occurrence of postoperative pain may be related to the patient′s gastric type pearson and changes in the position of the puncture point at the empty gastric state.

Objective:To observe the compatibility of Xiaoaiping injection and insulin for injection in glucose infusion. Methods:The changes in appearance, particles and pH of the mixture in 24h at ambient temperature were observed. The concentration of chloro-genic acid and insulin were determined by HPLC. Results:There were no obvious changes in appearance, pH, precipitation or turbidi-ty generation, and the contents of insulin and chlorogenic acid kept stable in 12 h. The number of insoluble particles was stable in 12 h, and then gradually increased in 24 h. Conclusion:Xiaoaiping injection and insulin in 5％ glucose infusion is stable in 12h under the testing conditions.

Objective　To analyze the CT features and diagnostic valuation of endometriosis. Methods 　The CT features in 26 cases with endometriosis confirmed by operation and pathology were retrospectively studied. Results　The endometriosis deposited in the overly （n=16）,wall of bladder （n=2）,uterus （n=5）,and Vagina （n=3）.On CT,the lesion could appear as a round or oval or irregular,Cystic or solid or mixed mass,with unclear margin,and certain adhesion to surrounding sssstructures.There were some small cysts within the mass.On enhanced CT,the cystic margin and solid part of the mass could be enhanced. Conclusion　CT features with clinical information,it is possible to diagnosis endometriosis.