Objective To prepare decellularized scaffolds from rabbit cartilage at various concentrations and assess their physicochemical properties and compatibility with stem cells to provide an experimental basis for cartilage repair.Methods Bone marrow mesenchymal stem cells(BMSCs)were cultured using the Percoll density gradient separation method,and this was followed by flow cytometric analysis and testing of their osteogenic and chondrogenic differentiation capabilities.Cartilage pieces were excised from rabbit knees and hip joints and subjected to physical crushing,repeated freeze-thaw cycles,and mixed enzymatic digestion for decellularization.To compare and observe the physicochemical properties of the decellularized scaffolds at different concentrations,three groups of scaffolds(labelwd A,B,and C)were designed with concentrations of 100％,50％and 30％,with three replicates each.Third-generation PKH26-labeled BMSCs were seeded onto optimally concentrated scaffolds and cultured for 1 week to observe cell growth.Results Flow cytometry detected BMSC surface antigens with positive expression of CD44 and CD90 and negative expression of CD45.Osteogenic induction stained with alizarin red showed red calcific nodules,and chondrogenic induction stained with alcian blue showed blue cartilaginous nodules.No apparent cell morphology was observed in the three groups of scaffolds stained with hematoxylin-eosin,and toluidine blue.There was a significant difference in DNA concentration between decellularized samples and non-decellularized scaffolds(P＜0.05).The content of glycosaminoglycans was slightly lower than the normal values.Significant differences were observed between the three groups of scaffolds in terms of pore size,water absorption,porosity,tensile strength,and Young's modulus(P＜0.05).After co-cultivation of stem cells with the scaffolds,cell adhesion was found to be good.Conclusions Percoll density gradient separation can obtain high-purity rabbit BMSCs,and the mixed decellularization method is superior.Group B scaffolds were the most suitable for tissue-engineered cartilage repair.BMSCs cultured in vitro grew well on Group B scaffolds.

Coronary microcirculation disorder after myocardial ischemia reperfusion （MIR） is a prominent problem in the treatment of coronary heart disease. According to the physiological commonality between “collaterals-sweat pore qi and fluid” and coronary microcirculation， and the evolution of the course of MIR， it is believed that “heart collateral stasis obstruction， sweat pore constraint and block” is the cause of coronary microcirculation disorder. The evolution of the pathogenesis can be divided into three periods. During the myocardial ischemia period， the pathogenesis is heart collaterals obstruction and sweat pores empty， while during the ischemia reperfusion period， it is internal formulation of deficiency wind， spasms of collaterals or slight heart collaterals obstruction； in the coronary microcirculation disorder period， sweat pores constraint and block， constraint transforming into heat， qi and fluid failing to diffuse are the pathogenesis. The corresponding treatment principle is assisting dredge with supplementation， and supplementing deficiency to dispel stasis； treating wind and blood simultaneously， and extinguishing wind to arrest convulsion； clearing heat and cooling blood， and diffusing qi and unblocking qi and fluid. Moreover， it is recommended to treat the heart and lungs simultaneously， and regulate the heart and liver at the same time.

Objective:To investigate the effects of baicalein combined with 5-FU on the proliferation, apoptosis and migration of small cell lung cancer H466 cells, and further to explore its sensitization mechanism.Methods:H466 cells were cultured in vitro and divided into blank control group, baicalein single treatment group, 5-FU single treatment group and combined treatment group. CCK-8 experiment was used to detect cell survival rate in each group; Flow cytometry was used to detect cell apoptosis and changes in ROS levels; Western blot was used to detect changes in the protein expression of the MAPK pathway.Results:CCK-8 results showed that the survival rates of the four groups of cells after 24 h treatment were 114.3% ± 2.8%, 79.8% ± 3.4%, 57.6% ± 1.8%, and 40.3% ± 2.7%. Compared with the other three groups, the combined treatment group had stronger inhibitory effect on the proliferation of H446 cells, and the difference was statistically significant ( P<0.001) . The proportion of apoptotic H446 cells in the combined treatment group was 49.2%±3.8%, which was significantly different from 33.9%±4.3% in the 5-FU single treatment group, and the difference was statistically significant ( P<0.001) . The inhibition rate of H446 cell migration in the combined treatment group was higher than that in the 5-FU single treatment group, and the difference was statistically significant ( P <0.001) . Combined treatment of baicalein and 5-FU can up-regulate ROS levels in H446 cells, thereby regulating the MAPK signaling pathway. Conclusion:The combined use of baicalein and 5-FU can increase the level of ROS in lung cancer H446 cells, activate the JNK and p38 signaling pathways, inhibit the ERK signaling pathway, and enhance 5-FU's proliferation inhibition, apoptosis induction and migration inhibition effects on H446 cells, which provides a certain experimental basis for the application of baicalein in small cell lung cancer.

Objective:To study the effect of hyperbaric oxygen（HBO）combined with vinorelbine（NVB）on the sensitivity of non-small cell lung cancer A549 cells，and to explore its mechanism.Methods:After the completion of culture，A549 cells were divided into four groups：blank group，HBO group，NVB group，and HBO+NVB group，with no treatment，0.2 MPa HBO treatment，IC 50 NVB treatment，and 0.2 MPa HBO + IC 50 NVB treatment，respectively. Cell Counting Kit-8（CCK-8）assay was used to detect the cell prolife-ration. Flow cytometry was adopted to detect cell apoptosis. Transwell experiment was used to detect cell migration. And Western blotting was used to detect the expression changes of A549 cell-related proteins. Results:The IC 50 value of NVB on A549 cells was 13.68 μmol/L. The inhibitory rates were 0，4.1%，46.60%，and 68.40% in the blank，HBO，NVB，and HBO+NVB groups，respectively，and the rate in the HBO+NVB group was significantly higher than those in the other three groups（ P<0.05 or P<0.01）. The apoptotic rates were 3.99%，7.63%，36.92%，and 56.49% in the blank，HBO，NVB，and HBO+NVB groups，respectively，and the rate in the HBO+NVB group was significantly higher than those in the other three groups（ P<0.05 or P<0.01）. Compared with the other three groups，the expression level of Bcl-2 in A549 cells in the HBO+NVB group was decreased，while the expression levels of Bad and Caspase-3 were increased（ P<0.01）. The percentages of cells inhibited from migrating in each group were 0，8.90%，42.20%，and 59.40%，respectively，and the HBO+NVB group had the lowest cell migration rate compared with the other three groups（ P<0.05 or P<0.01）. Compared with the other three groups，the expression level of E-Cadherin in the HBO+NVB group was increased，while the expression levels of N-Cadherin，P-glycoprotein（P-gp），and Multidrug resistance protein（MRP）were significantly decreased（ P<0.01）. Conclusion:HBO can increase the sensitivity of A549 cells to NVB by regulating the proteins related to drug resistance，cell migration，and cell apoptosis.

Objective:To study the effect of hyperbaric oxygen（HBO）combined with vinorelbine（NVB）on the sensitivity of non-small cell lung cancer A549 cells，and to explore its mechanism.Methods:After the completion of culture，A549 cells were divided into four groups：blank group，HBO group，NVB group，and HBO+NVB group，with no treatment，0.2 MPa HBO treatment，IC 50 NVB treatment，and 0.2 MPa HBO + IC 50 NVB treatment，respectively. Cell Counting Kit-8（CCK-8）assay was used to detect the cell prolife-ration. Flow cytometry was adopted to detect cell apoptosis. Transwell experiment was used to detect cell migration. And Western blotting was used to detect the expression changes of A549 cell-related proteins. Results:The IC 50 value of NVB on A549 cells was 13.68 μmol/L. The inhibitory rates were 0，4.1%，46.60%，and 68.40% in the blank，HBO，NVB，and HBO+NVB groups，respectively，and the rate in the HBO+NVB group was significantly higher than those in the other three groups（ P<0.05 or P<0.01）. The apoptotic rates were 3.99%，7.63%，36.92%，and 56.49% in the blank，HBO，NVB，and HBO+NVB groups，respectively，and the rate in the HBO+NVB group was significantly higher than those in the other three groups（ P<0.05 or P<0.01）. Compared with the other three groups，the expression level of Bcl-2 in A549 cells in the HBO+NVB group was decreased，while the expression levels of Bad and Caspase-3 were increased（ P<0.01）. The percentages of cells inhibited from migrating in each group were 0，8.90%，42.20%，and 59.40%，respectively，and the HBO+NVB group had the lowest cell migration rate compared with the other three groups（ P<0.05 or P<0.01）. Compared with the other three groups，the expression level of E-Cadherin in the HBO+NVB group was increased，while the expression levels of N-Cadherin，P-glycoprotein（P-gp），and Multidrug resistance protein（MRP）were significantly decreased（ P<0.01）. Conclusion:HBO can increase the sensitivity of A549 cells to NVB by regulating the proteins related to drug resistance，cell migration，and cell apoptosis.

0.05).There were obvious expression of PKC in ischemic preconditioning group,drug pretreatment+ ischemic preconditioning group and drug pretreatment group,but the rest groups not.Conclusion Shenyuandan was able to reduce the infarct size of myocardial ischemia in rats and had a pharmacological preconditioning-like myocardial protection,its mechanism may be related to PKC-mediated mechanism.