Objective To observe the value of MRI quantified uterine indictors in late pregnancy for predicting uterine inertia in delivery.Methods A total of 182 pregnant women were retrospectively collected and were divided into uterine inertia group(inertia group,n=67)and non-uterine inertia group(non-inertia group,n=115)based on delivery or cesarean section records.MRI quantified uterine indicators were compared between groups,and those being statistically different were included to build a multivariate logistic regression model for predicting uterine inertia in delivery.The predictive performance of this model was evaluated using receiver operating characteristic(ROC)curve and the area under the curve(AUC).Results The inertia group had more previous uterine surgeries,also higher proportion of in vitro fertilization and embryo transfer(IVF-ET)and cesarean sections compared to the non-inertia group(all P＜0.05).In late pregnancy,the longitudinal diameter of the uterus,cervical length and the thickness of the myometrium at the placental attachment site in inertia group were larger than those in non-inertia group(all P＜0.05).Increased times of previous uterine surgeries,undergoing IVF-ET,as well as increased longitudinal diameter of the uterus and cervical length in late pregnancy were all independent predictors of uterine inertia in delivery(all P＜0.05).AUC of the multivariate logistic regression model established based on the above factors for predicting uterine inertia in delivery was 0.733.Conclusion MRI quantified uterine indictors in late pregnancy could be used to predict uterine inertia in delivery.

Objective To observe the value of MRI quantified uterine indictors in late pregnancy for predicting uterine inertia in delivery.Methods A total of 182 pregnant women were retrospectively collected and were divided into uterine inertia group(inertia group,n=67)and non-uterine inertia group(non-inertia group,n=115)based on delivery or cesarean section records.MRI quantified uterine indicators were compared between groups,and those being statistically different were included to build a multivariate logistic regression model for predicting uterine inertia in delivery.The predictive performance of this model was evaluated using receiver operating characteristic(ROC)curve and the area under the curve(AUC).Results The inertia group had more previous uterine surgeries,also higher proportion of in vitro fertilization and embryo transfer(IVF-ET)and cesarean sections compared to the non-inertia group(all P＜0.05).In late pregnancy,the longitudinal diameter of the uterus,cervical length and the thickness of the myometrium at the placental attachment site in inertia group were larger than those in non-inertia group(all P＜0.05).Increased times of previous uterine surgeries,undergoing IVF-ET,as well as increased longitudinal diameter of the uterus and cervical length in late pregnancy were all independent predictors of uterine inertia in delivery(all P＜0.05).AUC of the multivariate logistic regression model established based on the above factors for predicting uterine inertia in delivery was 0.733.Conclusion MRI quantified uterine indictors in late pregnancy could be used to predict uterine inertia in delivery.

Objective To prepare decellularized scaffolds from rabbit cartilage at various concentrations and assess their physicochemical properties and compatibility with stem cells to provide an experimental basis for cartilage repair.Methods Bone marrow mesenchymal stem cells(BMSCs)were cultured using the Percoll density gradient separation method,and this was followed by flow cytometric analysis and testing of their osteogenic and chondrogenic differentiation capabilities.Cartilage pieces were excised from rabbit knees and hip joints and subjected to physical crushing,repeated freeze-thaw cycles,and mixed enzymatic digestion for decellularization.To compare and observe the physicochemical properties of the decellularized scaffolds at different concentrations,three groups of scaffolds(labelwd A,B,and C)were designed with concentrations of 100％,50％and 30％,with three replicates each.Third-generation PKH26-labeled BMSCs were seeded onto optimally concentrated scaffolds and cultured for 1 week to observe cell growth.Results Flow cytometry detected BMSC surface antigens with positive expression of CD44 and CD90 and negative expression of CD45.Osteogenic induction stained with alizarin red showed red calcific nodules,and chondrogenic induction stained with alcian blue showed blue cartilaginous nodules.No apparent cell morphology was observed in the three groups of scaffolds stained with hematoxylin-eosin,and toluidine blue.There was a significant difference in DNA concentration between decellularized samples and non-decellularized scaffolds(P＜0.05).The content of glycosaminoglycans was slightly lower than the normal values.Significant differences were observed between the three groups of scaffolds in terms of pore size,water absorption,porosity,tensile strength,and Young's modulus(P＜0.05).After co-cultivation of stem cells with the scaffolds,cell adhesion was found to be good.Conclusions Percoll density gradient separation can obtain high-purity rabbit BMSCs,and the mixed decellularization method is superior.Group B scaffolds were the most suitable for tissue-engineered cartilage repair.BMSCs cultured in vitro grew well on Group B scaffolds.

Objective To observe the value of placental MRI morphological indicators for diagnosing placenta accreta spectrum(PAS).Methods Totally 214 pregnant women who underwent prenatal placental MR examination were retrospectively enrolled and divided into PAS group(n=110)and non PAS group(n=104)based on the presence of PAS or not,also into PAS subgroup and non PAS subgroup during different weeks of pregnancy.Clinical and MRI data were compared between groups and subgroups,and the value of placental MRI morphological indicators for diagnosing PAS was analyzed.Results Significant differences of pregnant women's age,gestational week at MR examination,delivery gestational week,times of pregnancies,deliveries,cesarean sections,miscarriages and uterine clearances,as well as proportion of placenta praevia were found between groups(all P＜0.05).During 28-32 weeks and 32+1-36 weeks of pregnancy,significant differences of placental diameter,area and volume were found between subgroups(all P＜0.05),while no significant differences of placental MRI morphological indicators at gestational age＞36 weeks was found between subgroups(all P＞0.05).The area under the curve(AUC)of placental diameter,area and volume for diagnosing PAS at 28-32 weeks of pregnancy was 0.749,0.729 and 0.791,respectively,at 32+1-36 weeks was 0.718,0.686 and 0.614,respectively,being not significantly different(all P＞0.05).Conclusion Placental MRI morphological indicators at 28-36 weeks of pregnancy were helpful for diagnosing PAS.

Objective To observe the value of placental MRI morphological indicators for diagnosing placenta accreta spectrum(PAS).Methods Totally 214 pregnant women who underwent prenatal placental MR examination were retrospectively enrolled and divided into PAS group(n=110)and non PAS group(n=104)based on the presence of PAS or not,also into PAS subgroup and non PAS subgroup during different weeks of pregnancy.Clinical and MRI data were compared between groups and subgroups,and the value of placental MRI morphological indicators for diagnosing PAS was analyzed.Results Significant differences of pregnant women's age,gestational week at MR examination,delivery gestational week,times of pregnancies,deliveries,cesarean sections,miscarriages and uterine clearances,as well as proportion of placenta praevia were found between groups(all P＜0.05).During 28-32 weeks and 32+1-36 weeks of pregnancy,significant differences of placental diameter,area and volume were found between subgroups(all P＜0.05),while no significant differences of placental MRI morphological indicators at gestational age＞36 weeks was found between subgroups(all P＞0.05).The area under the curve(AUC)of placental diameter,area and volume for diagnosing PAS at 28-32 weeks of pregnancy was 0.749,0.729 and 0.791,respectively,at 32+1-36 weeks was 0.718,0.686 and 0.614,respectively,being not significantly different(all P＞0.05).Conclusion Placental MRI morphological indicators at 28-36 weeks of pregnancy were helpful for diagnosing PAS.