Objective To investigate the relationship between serum thioredoxin reductase 1(TXNRD1),p21-activated kinase 1(PAK1)levels and insulin resistance(IR)and adverse pregnancy outcomes(APO)in pa-tients with gestational diabetes mellitus(GDM).Methods Eighty patients with GDM admitted to Baoji Third Hospital from January 2019 to January 2024 were selected as the GDM group.They were divided into the APO group(25 cases)and the non-APO group(55 cases)based on whether APO occurred.Eighty healthy pregnant women who came to the hospital for prenatal examination recently in a 1∶1 ratio were selected as the control group.The levels of serum TXNRD1 and PAK1 were detected by enzyme-linked immunosorbent assay.The correlation between the levels of serum TXNRD1 and PAK1 in patients with GDM and the homeo-static model assessment of insulin resistance(HOMA-IR)was analyzed through Pearson correlation analysis.Multivariate unconditional Logistic regression was used to analyze the relationship between serum TXNRD1 and PAK1 levels and APO in patients with GDM,and the receiver operating characteristic(ROC)curve was used to analyze the predictive efficacy of serum TXNRD1 and PAK1 levels for them.Results Compared with the control group,HOMA-IR and the levels of serum TXNRD1 and PAK1 in the GDM group increased,and the difference was statistically significant(P<0.05).Pearson correlation analysis showed that the levels of serum TXNRD1 and PAK1 in patients with GDM were positively correlated with HOMA-IR(r=0.783,0.790,P<0.001).The results of multivariate unconditional Logistic regression analysis showed that high TXNRD1 and high PAK1 were independent risk factors for APO in patients with GDM(P<0.05).The re-sults of ROC curve analysis showed that the area under the curve(AUC)of serum TXNRD1 and PAK1 levels alone and in combination for predicting APO in GDM patients was 0.785,0.789,and 0.900,respectively.The combined AUC of serum TXNRD1 and PAK1 levels for predicting APO in GDM patients was the largest(Z=2.148,2.454,P=0.032,0.014).Conclusion The levels of serum TXNRD1 and PAK1 in patients with GDM are related to IR and APO.The combination of levels of serum TXNRD1 and PAK1 have a certain pre-dictive efficacy for APO in patients with GDM.

Objective:To investigate the effect of ultrasound combined with transcutaneous electrical nerve stimulation on pain and function of knee joint in patients with knee osteoarthritis.Method:A total of 122 patients with knee osteoarthritis graded 1 to 3 according to Kellgren-Lawrence (K-L) classification were randomly divided into the experimental group (n=61)and the control group(n=61). The pa-tients in the experimental group were treated with ultrasound combined with transcutaneous electrical nerve stim-ulation on the basis of conventional rehabilitation treatment,and the patients in the control group were only treated with ultrasound on the basis of conventional rehabilitation treatment. The Visual Analogue Scale (VAS) for pain,Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC),walking ability rating scale and 6-minute walk distance were used to evaluate the pain and function of knee joint before treatment,4 weeks after treatment and 3 months after treatment.Result:Before treatment,there was no significant difference between the experimental group and the controlgroup. After 4 weeks of treatment,the VAS score,WOMAC pain and function scores of the two groups were significantly decreased (P<0.05). Improvements in walking ability scores and 6-minute walk distance were significant in both groups (P<0.05). No significant changes were observed in the WOMAC stiffness score between the two groups (P>0.05),The VAS score,WOMAC pain and function score,walking ability score and 6-minute walk distance in the experimental group had statistical significance when compared with those in the control group (P<0.05). At follow-up,except for the WOMAC stiffness score and function score,there were significant differences when compared with those before treatment in the two groups (P<0.05). The VAS score,WOMAC pain score,walking ability score and 6-minute walk distance in the experi-mental group were significantly improved than those in the control group (P<0.05).Conclusion:Ultrasound combined with transcutaneous electrical nerve stimulation could significantly relieve pain,enhances walking function and improve walking ability in people with knee osteoarthritis,but does not have a marked effect on joint stiffness.

Objective:To investigate the effect of ultrasound combined with transcutaneous electrical nerve stimulation on pain and function of knee joint in patients with knee osteoarthritis.Method:A total of 122 patients with knee osteoarthritis graded 1 to 3 according to Kellgren-Lawrence (K-L) classification were randomly divided into the experimental group (n=61)and the control group(n=61). The pa-tients in the experimental group were treated with ultrasound combined with transcutaneous electrical nerve stim-ulation on the basis of conventional rehabilitation treatment,and the patients in the control group were only treated with ultrasound on the basis of conventional rehabilitation treatment. The Visual Analogue Scale (VAS) for pain,Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC),walking ability rating scale and 6-minute walk distance were used to evaluate the pain and function of knee joint before treatment,4 weeks after treatment and 3 months after treatment.Result:Before treatment,there was no significant difference between the experimental group and the controlgroup. After 4 weeks of treatment,the VAS score,WOMAC pain and function scores of the two groups were significantly decreased (P<0.05). Improvements in walking ability scores and 6-minute walk distance were significant in both groups (P<0.05). No significant changes were observed in the WOMAC stiffness score between the two groups (P>0.05),The VAS score,WOMAC pain and function score,walking ability score and 6-minute walk distance in the experimental group had statistical significance when compared with those in the control group (P<0.05). At follow-up,except for the WOMAC stiffness score and function score,there were significant differences when compared with those before treatment in the two groups (P<0.05). The VAS score,WOMAC pain score,walking ability score and 6-minute walk distance in the experi-mental group were significantly improved than those in the control group (P<0.05).Conclusion:Ultrasound combined with transcutaneous electrical nerve stimulation could significantly relieve pain,enhances walking function and improve walking ability in people with knee osteoarthritis,but does not have a marked effect on joint stiffness.

Animals ; SARS-CoV-2 ; Antibodies, Neutralizing ; Macaca mulatta ; COVID-19/prevention & control* ; Antibodies, Viral ; Vaccines

Objective To investigate the relationship between SOCS-3 gene polymorphism and abnormal glucose metabolism in Xinjiang Uygur population.Methods According to different plasma glucose levels,1232individuals in Xinjiang Hetian area were divided into 3 groups,451 patients with pre-diabetes,252 patients with type 2 diabetes mellitus,and 529 healthy people as normal controls.Based on HapMap,the polymorphisms rs12953258,rs4969168,rs9914220 were selected as haplotypes,tagging SNP (htSNP) sufficiently covering the genetic variation of the whole gene.Associations of rs12953258,rs4969168,and rs9914220 within SOCS-3 with abnormal glucose metabolism in three groups of Xinjiang Uygur population were examined ; The genotype and allele frequencies and relative clinic data were compared among groups.Results The type 2 diabetes mellitus study with 451 individuals showed that the homozygosity for the C allele of rs12953258 polymorphism of SOCS-3 was associated with increased diabetes risk(OR=1.756,95％ CI 1.168-2.640).In addition,association between rs4969168 or rs9914220 and abnormal glucose metabolism in the Xinjiang Uygur population was not found.Age,total cholesterol,and body mass index were risk factors of diabetes mellitus,total cholesterol and low high density lipoprotein-cholesterol were risk factors of pre-diabetes in Uygur people.Conclusions The C allele of rs12953258 polymorphism of SOCS-3 gene may be an independent risk factor for abnormal glucose metabolism in Xinjiang Uygur population.

Objective To evaluate the role of transbrochial needle aspiration (TBNA) in the diagnosis of patients with enlarged mediastinal and/or hilar lymph node. Methods Patients with mediastinal and/or hilar lymphoadenopathy proven by CT scan were eligible for TBNA as reported. All specimens were directly and instantly smeared for pathological examination. Results From June 1 2004 to December 31 2007, 164 patients were examined: including 80 lung cancers, 69 lung bengin diseases, 2 other malignancy tumor, and 13 without definite diagnosis. Total 260 lymph nodes were punctured. TBNA procedures were successfully carried out in 445/463(96.1%). Sensitivity of TBNA was 82. 5 % (66/80) in patients who had been proven to suffer from bronchogenic carcinoma. There were 25 patients that diagnosis of lung cancer was pathologically determined by TBNA only. A total of 122 lymph nodes in the 80 lung cancer patients were aspirated by TBNA with a positive rate of 65. 6% (80/122). Severe complications were rare except small amount of bleeding at the TBNA site (100/164, 61.0%). From June 1 2006 to December 31 2007, lymph node tissues able to make histology diagnosis were yield in 73.5% (64/87) patients. Through histology pathology, the sensitivities of TBNA were 53. 3% (8/15) for sarcoidosis and 78.6% (33/42) for lung cancer. Conclusion TBNA is quite safe and helpful in diagnosis and staging of bronchogenic carcinoma, and in diagnosis of benign lung diseases.

In order to explore the value of combined detection of atypical lymphocytes (ATL) and transaminase (alanine aminotransferase, ALT; asparate aminotransferase, AST) in the diagnosis of infectious mononucleosis (IM), The data of blood routine and liver function were collected from 54 IM patients, 34 acute hepatitis (AH) patients, 44 upper respiratory infection (URI) patients in Union Hospital during March 2002 to March 2005. Same data were also collected from 40 healthy children as normal control. These data were analyzed retrospectively. Both proportion of atypical lymphocytes and enzyme activity of transaminase were elevated simultaneously (ALT > 40 IU/L, AST > 45 IU/L) in 57.4% (31/54) IM patients. There was significant difference (P < 0.01) between IM group and the other groups. Combined detection of atypical lymphocytes and transaminase can be regarded as a diagnostic marker of infectious mononucleosis.

In order to explore the value of combined detection of atypical lymphocytes (ATL) and transaminase (alanine aminotransferase, ALT; asparate aminotransferase, AST) in the diagnosis of infectious mononucleosis (IM), The data of blood routine and liver function were collected from 54 IM patients, 34 acute hepatitis (AH) patients, 44 upper respiratory infection (URI) patients in Union Hospital during March 2002 to March 2005. Same data were also collected from 40 healthy children as normal control. These data were analyzed retrospectively. Both proportion of atypical lymphocytes and enzyme activity of transaminase were elevated simultaneously (ALT＞40 IU/L,AST＞45 IU/L) in 57.4% (31/54) IM patients. There was significant difference (P＜0.01) between IM group and the other groups. Combined detection of atypical lymphocytes and transaminase can be regarded as a diagnostic marker of infectious mononucleosis.