Objective:To analyze the effects of parenting care map of premature infants on parenting competence and parenting stress of primipara, with a view to providing a theoretical basis for the development, implementation and modification of clinical interventions.Methods:Convenient sampling method was used to select primigravid women who gave birth in Lishui Maternal and Child Health Hospital from January 2023 to January 2024 for randomized controlled study, which was divided into the observation group and the control group according to the random number table. The control group received routine nursing, and the observation group guided the parents of premature infants to draw the child care map together on the basis of the control group. The scores of Chinese version of parenting sense of competence scale ( C-PSOC ) and Chinese version of Simplified Parenting Stress Index ( PSI-SF ) before and after intervention were compared.Results:A total of 120 cases of primiparous women who met the inclusion and exclusion criteria were enrolled, 60 cases in the control group and 60 cases in the observation group. The age of the control group was (26.25 ± 3.93) years, and the age of the observation group was (25.86 ± 4.22) years. Before the intervention, the difference between the C-PSOC dimension scores and total scores, PSI-SF dimension scores and total scores of the 2 groups was not statistically significant (all P>0.05). After the intervention, the maternal C-PSOC efficacy, satisfaction dimension scores and total scores of primiparous women in the observation group were (32.05 ± 6.27), (40.29 ± 8.19), and (72.23 ± 9.39) points, respectively, which were higher than those of the control group, which were (28.46 ± 6.47), (37.08 ± 8.06), and (65.53 ± 10.24) points, and the differences between the 2 groups were all statistically significant ( t = 3.09, 2.16, 3.74, all P<0.05). After the intervention, the PSI-SF scores and total scores of primiparous women in the observation group on the dimensions of parental distress, personal interaction disorder, and difficult child were (24.43 ± 5.44), (27.51 ± 6.53), (29.47 ± 5.82), and (81.54 ± 10.38) points, respectively, which were lower than those of the control group (28.57 ± 5.52), (30.19 ± 7.02), (32.78 ± 5.37), and (90.97 ± 10.12) points, and the differences between the 2 groups were all statistically significant ( t = 4.41, 2.17, 3.23, 5.04, all P<0.05). Conclusions:Premature child care map can effectively improve the parenting sense of competence of primiparas and reduce their parenting pressure. It is a convenient and effective nursing intervention tool.

Objective:To analyze the effects of parenting care map of premature infants on parenting competence and parenting stress of primipara, with a view to providing a theoretical basis for the development, implementation and modification of clinical interventions.Methods:Convenient sampling method was used to select primigravid women who gave birth in Lishui Maternal and Child Health Hospital from January 2023 to January 2024 for randomized controlled study, which was divided into the observation group and the control group according to the random number table. The control group received routine nursing, and the observation group guided the parents of premature infants to draw the child care map together on the basis of the control group. The scores of Chinese version of parenting sense of competence scale ( C-PSOC ) and Chinese version of Simplified Parenting Stress Index ( PSI-SF ) before and after intervention were compared.Results:A total of 120 cases of primiparous women who met the inclusion and exclusion criteria were enrolled, 60 cases in the control group and 60 cases in the observation group. The age of the control group was (26.25 ± 3.93) years, and the age of the observation group was (25.86 ± 4.22) years. Before the intervention, the difference between the C-PSOC dimension scores and total scores, PSI-SF dimension scores and total scores of the 2 groups was not statistically significant (all P>0.05). After the intervention, the maternal C-PSOC efficacy, satisfaction dimension scores and total scores of primiparous women in the observation group were (32.05 ± 6.27), (40.29 ± 8.19), and (72.23 ± 9.39) points, respectively, which were higher than those of the control group, which were (28.46 ± 6.47), (37.08 ± 8.06), and (65.53 ± 10.24) points, and the differences between the 2 groups were all statistically significant ( t = 3.09, 2.16, 3.74, all P<0.05). After the intervention, the PSI-SF scores and total scores of primiparous women in the observation group on the dimensions of parental distress, personal interaction disorder, and difficult child were (24.43 ± 5.44), (27.51 ± 6.53), (29.47 ± 5.82), and (81.54 ± 10.38) points, respectively, which were lower than those of the control group (28.57 ± 5.52), (30.19 ± 7.02), (32.78 ± 5.37), and (90.97 ± 10.12) points, and the differences between the 2 groups were all statistically significant ( t = 4.41, 2.17, 3.23, 5.04, all P<0.05). Conclusions:Premature child care map can effectively improve the parenting sense of competence of primiparas and reduce their parenting pressure. It is a convenient and effective nursing intervention tool.

Objective To investigate the changes in psychological state after occupational exposure in the AIDS occupational exposure population and related influencing factors,and to provide baseline data and a basis for related departments to conduct mental health prevention and intervention for personnel with occupational AIDS exposure.Methods AIDS risk assessment was performed for all personnel with occupational AIDS exposure in 2014 in Nanning,China,and the Symptom Checklist-90 (SCL-90) psychological scale was used for psychological state evaluation at 24 hours,1 week,and 3 months after occupational exposure in all persons who met the research criteria.Results Most of the persons with occupational AIDS exposure came from secondary and tertiary hospitals (85％),and nurses accounted for the highest percentage (78.3％).The age ranged from 21 to 50 years,and the mean age was 31.02+7.92 years.The persons with occupational AIDS exposure aged 20～29 years accounted for the highest percentage (51.6％),and most persons (76.7％) graduated from junior colleges.Compared with the adult norm,there was significant increases in the total psychological score and the number of positive items after occupational exposure (P＜0.05).The scores of all items at 24 hours were significantly higher than those at the other time points,and the scores of all items gradually decreased over time (F=227.24,267.57,and 287.46,P＜0.05).Compared with the adult norm,there were significant increases in the factor points at 24 hours and significant reductions in the factor points at 3 months (P＜0.05).Compared with those at 24 hours,the factor scores at 3 months decreased significantly (P＜0.05).Conclusion Occupational AIDS exposure affects the mental status of related personnel,and the mental status at 24 hours after exposure is poor.Related departments should provide corresponding psychological counseling for the occupational exposure population at different exposure times.

Objective To investigate the changes in psychological state after occupational exposure in the AIDS occupational exposure population and related influencing factors,and to provide baseline data and a basis for related departments to conduct mental health prevention and intervention for personnel with occupational AIDS exposure.Methods AIDS risk assessment was performed for all personnel with occupational AIDS exposure in 2014 in Nanning,China,and the Symptom Checklist-90 (SCL-90) psychological scale was used for psychological state evaluation at 24 hours,1 week,and 3 months after occupational exposure in all persons who met the research criteria.Results Most of the persons with occupational AIDS exposure came from secondary and tertiary hospitals (85％),and nurses accounted for the highest percentage (78.3％).The age ranged from 21 to 50 years,and the mean age was 31.02+7.92 years.The persons with occupational AIDS exposure aged 20～29 years accounted for the highest percentage (51.6％),and most persons (76.7％) graduated from junior colleges.Compared with the adult norm,there was significant increases in the total psychological score and the number of positive items after occupational exposure (P＜0.05).The scores of all items at 24 hours were significantly higher than those at the other time points,and the scores of all items gradually decreased over time (F=227.24,267.57,and 287.46,P＜0.05).Compared with the adult norm,there were significant increases in the factor points at 24 hours and significant reductions in the factor points at 3 months (P＜0.05).Compared with those at 24 hours,the factor scores at 3 months decreased significantly (P＜0.05).Conclusion Occupational AIDS exposure affects the mental status of related personnel,and the mental status at 24 hours after exposure is poor.Related departments should provide corresponding psychological counseling for the occupational exposure population at different exposure times.

OBJECTIVETo construct eukaryotic expression vectors for different domains (V and VC1) of the extracellular region of the receptor of advanced glycation end products (RAGE) and investigate the roles of these domains in prostate cancer.

METHODSThe coding sequence of V and VC1 domains was amplified from the plasmid pcDNA3-HA-RAGE by PCR and cloned into the pcDNA3-HA vector following routine procedures. After identification by PCR and sequencing, the vectors including V and VC1 domains were transfected into PC-3 cells. Western blotting and immunofluorescence were used to detect the expression and distribution of the expressed products in transfected PC-3 cells.

RESULTSThe expression vectors containing V and VC1 domains of RAGE were successfully constructed as confirmed by PCR and DNA sequence analysis. The V and VC1 domains of RAGE were highly expressed and showed a cytoplasmic distribution in transfected PC-3 cells.

CONCLUSIONThe constructed eukaryotic expression vectors for V and VC1 domains of RAGE can be efficiently expressed in prostate cancer cells.

Cell Line, Tumor ; Cloning, Molecular ; Genetic Vectors ; Humans ; Male ; Plasmids ; Prostatic Neoplasms ; genetics ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic ; metabolism ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; Sequence Analysis, DNA ; Transfection