BACKGROUND: The transcription factor POU2F1 regulates the expression levels of microRNAs in neoplasia. However, the miR-29b1/a cluster modulated by POU2F1 in gastric cancer (GC) remains unknown. METHODS: Gene expression in GC cells was evaluated using reverse-transcription polymerase chain reaction (PCR), western blotting, immunohistochemistry, and RNA in situ hybridization. Co-immunoprecipitation was performed to evaluate protein interactions. Transwell migration and invasion assays were performed to investigate the biological behavior of GC cells. MiR-29b1/a cluster promoter analysis and luciferase activity assay for the 3'-UTR study were performed in GC cells. In vivo tumor metastasis was evaluated in nude mice. RESULTS: POU2F1 is overexpressed in GC cell lines and binds to the miR-29b1/a cluster promoter. POU2F1 is upregulated, whereas mature miR-29b-3p and miR-29a-3p are downregulated in GC tissues. POU2F1 promotes GC metastasis by inhibiting miR-29b-3p or miR-29a-3p expression in vitro and in vivo . Furthermore, PIK3R1 and/or PIK3R3 are direct targets of miR-29b-3p and/or miR-29a-3p , and the ectopic expression of PIK3R1 or PIK3R3 reverses the suppressive effect of mature miR-29b-3p and/or miR-29a-3p on GC cell metastasis and invasion. Additionally, the interaction of PIK3R1 with PIK3R3 promotes migration and invasion, and miR-29b-3p , miR-29a-3p , PIK3R1 , and PIK3R3 regulate migration and invasion via the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway in GC cells. In addition, POU2F1 , PIK3R1 , and PIK3R3 expression levels negatively correlated with miR-29b-3p and miR-29a-3p expression levels in GC tissue samples. CONCLUSIONS The POU2F1 - miR-29b-3p / miR-29a-3p-PIK3R1 / PIK3R1 signaling axis regulates tumor progression and may be a promising therapeutic target for GC.
MicroRNAs/metabolism* ; Humans ; Stomach Neoplasms/pathology* ; Cell Line, Tumor ; Cell Movement/physiology* ; Phosphatidylinositol 3-Kinases/metabolism* ; Animals ; Mice ; Octamer Transcription Factor-1/metabolism* ; Mice, Nude ; Class Ia Phosphatidylinositol 3-Kinase/metabolism* ; Neoplasm Invasiveness ; Gene Expression Regulation, Neoplastic/genetics* ; Male ; Immunohistochemistry ; Female

This study aims to summarize the clinical features of subarachnoid hemorrhage(SAH)caused by intraspi-nal schwannoma by analyzing 19 cases of intraspinal schwannoma manifesting as SAH who were admitted to our hospital or reported in the literature.The patients in this study had the clinical manifestation of severe headache and vomiting,with-out the symptoms of spinal cord or nerve root compression.Lumbar puncture confirmed SAH,and cerebral digital subtrac-tion angiography did not show intracranial aneurysms or vascular malformations.Spinal cord MRI showed extramedullary intradural tumor in the cervical segment,and pathological biopsy results confirmed the diagnosis of schwannoma.Some cases reported in the literature not only presented with SAH but also had the symptoms of spinal cord and nerve root com-pression.The clinical features of this disease suggest that clinicians should clarify the presence or absence of the symp-toms or signs of nerve root or spinal cord compression in SAH patients with negative intracranial imaging findings and per-form reasonable examinations to exclude intraspinal lesions.Early diagnosis and complete tumor resection can not only pre-vent bleeding and recurrence,but also effectively avoid irreversible damage to the spinal cord and nerve roots caused by the compression of intradural lesions in the spinal canal,thereby helping to improve the prognosis of patients.

Objective: To evaluate the relationship between preoperative magnetic resonance imaging (MRI) examination and the success rate and prognosis of breast-conserving surgery in ductal carcinoma in situ (DCIS) patients. Methods: Patients with DCIS received surgery between January 2009 and January 2017 in the breast cancer medical record database of Shanghai Jiao Tong University were retrospectively analyzed. Patients who received preoperative MRI was defined as MRI group, who did not receive preoperative MRI was defined as non-MRI group. Results: A total of 210 DCIS patients were included, of which 167 cases in the MRI group and 43 cases in the non-MRI group, respectively. Initial negative resection margin rates were similar between MRI and non-MRI groups undergoing breast-conserving surgery (91.0% vs. 97.7%, P=0.142). There was also no significant difference in the success rate of breast-conserving surgery between MRI and non MRI groups (95.2% vs. 100.0%, P=0.143). In terms of prognosis, there was no significant difference in 10-year loco-regional recurrence rate (4.8% vs. 2.3%, P=0.426) and breast cancer related mortality (0.9% vs. 0.5%, P=0.668) between MRI and non-MRI groups. Conclusions: Preoperative breast MRI examination in patients with DCIS had no effect on the rate of negative resection margin, success rate of breast preservation and the prognosis of the patients.

Objective:To investigate the diagnostic value of ultrasound elastography combined with enhancer of Zeste homolog 2 (EZH2) and breast cancer susceptibility gene 1 (BRCA1) proteins in triple-negative breast cancer.Methods:A total of 116 breast cancer patients selected for treatment in our hospital from 2019 to 2021 were analyzed, and ER and PR were analyzed according to the expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2). Patients who were all HER2 negative were classified as TNBC group, with a total of 21 cases, and patients who were positive for ER, PR, and HER2 were classified as non-TNBC, a total of 95 cases. All enrolled patients underwent ultrasonic elastography examination. The positive expression of EZH2 and BRCA1 was detected by immunohistochemistry. Chi-square test was used to analyze the diagnostic value of ultrasound elastography combined with EZH2 and BRCA1 proteins in TNBC.Results:In the TNBC group, the proportion of smooth edges (35.09) and lobulation of lesions (9.52) was higher than that in the non-TNBC group (12.63, 5.26), while the proportion of burrs (28.57) and fuzziness (23.81) was lower than that in the non-TNBC group (50.53, 31.58). The proportion of calcifications (52.38) in the TNBC group was lower than that in the non-TNBC group (85.26). The non-attenuation ratio of rear echo (80.95) was higher than that of non-TNBC group (52.63). The proportion of blood flow grade enrichment (57.14) was lower than that in the non-TNBC group (63.16) (all P<0.05). The positive expression rate of EZH2 in the TNBC group (85.71) was higher than that of non-TNBC (63.16) ( P<0.001), and the positive expression rate of BRCA1 in the TNBC group (19.05) was lower than that of non-TNBC (55.79) ( P<0.05). The posterior echo non-attenuation ratio (88.89) and rich blood supply ratio (88.89) of EZH2 positive patients in the TNBC group were higher than those with negative (33.33, 0) (all P<0.05), and the rear echo non-attenuation ratio (25.00) and rich blood supply ratio (25.00) of BRCA1 positive in the TNBC group were lower than those with negative (52.94, 88.24) (all P<0.05) ; According to the pathological results, the sensitivity, specificity and accuracy of posterior echo for the diagnosis of TNBC were 25.37%, 91.84% and 53.44%, respectively. The sensitivity, specificity and accuracy of EZH2 were 23.08%, 92.11% and 45.69%, respectively. The sensitivity, specificity and accuracy of BRCA1 were 28.81%, 92.98% and 60.34%, respectively. The combined sensitivity, specificity and accuracy of the three were 53.13%, 95.24% and 83.62%, respectively. The sensitivity, specificity and accuracy of blood flow fraction for the diagnosis of TNBC were 25.81%, 90.74% and 56.03%, respectively. The sensitivity, specificity and accuracy of blood flow fraction, EZH2 and BRCA1 were 59.26%, 94.38% and 86.21%, respectively. Conclusion:The joint classification of the posterior echoes and blood flow in ultrasonic elastography combined with EZH2 and BRCA1 has important significance in the diagnosis of TNBC.

Objective To construct a non-punitive nursing adverse event management system and explore the effects of the system in the management of adverse events in primary hospitals. Methods By convenient sampling, 71 967 patients who were hospitalized in the first 2 years of the introduction of the adverse event reporting system from January 2015 to December 2016 were selected as the control group, the Department of Nursing unified adverse event table was sent to the hospital OA system, adverse events are reported to the Department of Nursing; a total of 73 002 patients hospitalized after the direct reporting system was enabled from January 2017 to December 2018 were the experimental group. Comparing the reported rate of adverse events, adverse event grading, categories, and fall/falling injury rates between the two groups. Results A total of 811 adverse events were reported in the experimental group, which was higher than 715 in the control group, the difference between the two groups was statistically significant (χ2=5.685,P=0.017). There was a statistically significant difference in the levels of adverse events incidence between the experimental group and the control group (P＜ 0.05). The number of cases of drug extravasation and specimen-related events increased in the experimental group, and the number of needlestick and scald events decreased, the difference between the two groups were statistically significant (P＜0.05). The incidence of Grade Ⅲ injury in the fall/fall of the test group was 3.1%(5/159), which was lower than that of the control group (11.6%,15/129), the difference between the two groups was statistically significant (P＜ 0.05). Conclusions The non-punitive direct reporting system is conducive to creating a safe culture atmosphere for hospital care, increasing the active reporting rate of adverse events, reducing the level of adverse events and injury, changing the composition ratio of adverse events, thereby improving the efficiency of nursing management and continuously improving the quality of care.

Objective Protective constraint quilt is designed by applying quality control circle activities to improve the property of warmth for visible dysphoria patients and to reduce the rate of unplanned extubation. Methods Quality control circle group finally designed a kind of protective constraint quilt under the theme of“improve the warmth retention property for visible dysphoria patients, reduce the rate of unplanned extubation”. The protective constraint quilt was used in 40 patients, whose treatment effects were compared with patients controlled by limb restraint strap, chest belt and restraint gloves before the implementation of quality control circle activities in terms of the damage rate of skin integrity, the extubation rate, the property of warmth and satisfaction of patients′ families. Results The protective constraint quilt was adjusted for 6 times by quality control circle group. After improvement, the satisfaction degree of patients′ families improved from 75. 0% to 95. 0%, the property of warmth was better and the extubation rate was lower than those of protective constraint quilt before improvement along with significant differences (P<0. 05). There was no significant difference in extremities blood circulation and skin integrity (P > 0. 05). Conclusions This activity improves group members′scientific thinking and innovation ability. The protective constraint quilt designed by quality control circle is effective to be applied to visible dysphoria patients. Satisfaction of patients′ families with this way of constraint is high. In conclusion, protective constraint quilt is worthy of popularizing and applying.