[Objective]To establish the high performance liquid chromatography(HPLC)characteristic chromatogram and multi-index contents determination method of Yunvjian,clarify the quantitative and qualitative transfer law from decoction pieces-references sample-granules.[Methods]The HPLC characteristic chromatogram of Yunvjian references sample and granules was established using Thermoscientific Syncronis aQ C18 chromatographic column(150 mm×4.6 mm,5 μm),acetonitrile(A)-0.1%phosphoric acid water(B)as mobile phase,detection wavelength as 240 nm,adopting gradient elution.Five index components,including rehmannioside D,neomangiferin,mangiferin,β-ecdysterone and acteoside,were selected to establish an HPLC method with detection wavelengths of 203 nm and 240 nm.The content of the index components and the transfer rate in different samples were calculated.[Results]The similarity of the HPLC characteristic chromatograms of 10 batches of the Yunvjian references samples and 3 batches of granules is all greater than 0.950.A total of 12 common peaks are attributed,and 6 of them are identified.All the 12 common peaks in the reference samples are stably transferred to the granules.The 5 index components showed excellent linearity(r≥0.9999)within their respective concentration ranges,and the average sample recovery rate is 99.16%～102.26%.The methodological investigation of the content determination meets the relevant requirements.During the process of preparing the Yunvjian from decoction pieces to granules,the transfer rates of the 5 components are 46.31%～52.30%,31.89%～41.46%,28.65%～33.85%,41.28%～48.59%and 49.59%～75.20%,respectively,all within the range of the average value±30%.[Conclusion]The HPLC characteristic chromatogram and content determination method established are accurate and reliable,and the quantitative and qualitative transfer law of decoction pieces-granules is relatively stable,which can provide an objective basis for the preparation development and quality control of Yunvjian.

[Objective]To investigate the mechanism of Wumen Gumibao Decoction in treating senile osteoporosis(SOP)of senescence accelerated mouse prone 6(SAMP6)mice.[Methods]SAM-resistant 1(SAMR1)mice was set as control group,SAMP6 mice were randomly divided into model group(SAMP6),Chinese medicine low dose group(SAMP6+Wumen Gumibao Decoction 7.5 g·kg-1)and Chinese medicine high dose group(SAMP6+Wumen Gumibao Decoction 15.0 g·kg-1),with 10 mice in each group.Mice in control group and model group were gavaged with equal volume of 0.9%sodium chloride solution,and the two groups of Chinese medicine were given Wumen Gumibao Decoction,once a day for 12 weeks.The changes of body mass were observed during the experiment.At the end of the experiment,hematoxylin-eosin(HE)staining was used to observe the pathological changes of the femur in mice.Micro computed tomography(Micro-CT)was used to observe the microstructure and parameters of the femur.The serum levels of calcium(Ca),phosphorus(P)was detected with biochemical method,and the serum levels of calcitonin(CT),procollagen type Ⅰ N-terminal propeptide(PⅠNP),bone γ-carboxyglutamic acid-containing protein(BGP),alkaline phosphatase(ALP),tartrate resistant acid phosphatase(TRACP),procollagen type Ⅰ C-terminal propeptide(PⅠCP),insulin-like growth factor-1(IGF-1),parathyroid hormone(PTH)were detected by enzyme-linked immunosorbent assay(ELISA).Immunohistochemical(IHC)staining was used to detect Wnt3α,phosphorylated-b-catenin(p-b-catenin),osteoprotegerin(OPG),receptor activator of nuclear factor-κB(RANK)and ligand of receptor activator of NF-κB(RANKL),secretory protein Dickkopf-1(DKK1)and glycogen synthase kinase 3b(GSK3b).[Results]Compared with control group,the cortical bone texture,direction and arrangement of bone cells in bone tissue of model group were disordered,and the trabecular structure was loose,showing a state of osteoporosis,bone microstructure parameters bone mineral density,bone volume/tissue volume ratio,trabecular number,trabecular thickness decreased significantly(P<0.05)and trabecular separation increased significantly(P<0.05).The serum levels of Ca,BGP,PⅠNP,CT and ALP were significantly decreased(P<0.05),and P,TRACP,PⅠCP and IGF-1 were significantly increased(P<0.05).In addition,the expression of Wnt3a as significantly down-regulated(P<0.05),and the expression of p-β-catenin,OPG,RANK,RANKL,DKK1 and GSK3β was significantly up-regulated(P<0.05).Compared with model group,Chinese medicine low and high dose groups can improve the pathological morphology of the femur to varying degrees,increase the number of bone trabeculae and reduced the fracture of bone trabeculae,and improved the related parameters of bone microstructure(P<0.05).The levels of serum Ca,CT,BGP,PⅠNP and ALP were increased(P<0.05),and the levels of P,TRACP,PⅠCP and IGF-1 were decreased(P<0.05),and PTH was increased(P<0.05).At the same time,it could up-regulate the expression of Wnt3α protein in the femur(P<0.05)and down-regulate the expression of p-β-catenin,OPG,RANK,RANKL,DKK1 and GSK3b protein(P<0.05).[Conclusion]Wumen Gumibao Decoction has the effect of significantly improving osteoporosis in SAMP6 mice,and its mechanism may be related to its regulation of Wnt/β-catenin and OPG/RANK/RANKL signaling pathways.

[Objective]To explore new pathways for cultural education in Chinese medicine institutions,delve deeply into the educational elements embedded in traditional Chinese medicine(TCM)culture,and establish a new educational system that gives full play to the advantages and characteristics of TCM culture.[Methods]Through the status analysis on TCM culture-based education in Chinese medicine institutions,based on the exploration of the main bodies,content,forms and effectiveness of education,concrete ideas and practical pathways for reforming the curriculum system and the practice system were advanced,and the achievements of student cultivation were listed as evidence.[Results]The practice of cultivating students through TCM culture in Chinese medicine institutions was confronted with multiple issues,including the need to strengthen collaborative education,the need to integrate education and teaching content,and the necessity to enhance the talent-nurturing mechanism through campus culture.Zhejiang Chinese Medical University has fostered a campus culture rich in TCM characteristics by establishing a curriculum and practical system that merges TCM culture into the five dimensions of education and constructs a comprehensive and interconnected support system.The social recognition won by the students at this university has fully proved the effectiveness in enhancing students'comprehensive qualities and fostering cultural confidence.[Conclusion]To fulfill the missions of moral and cultural education,Chinese medical universities must establish a new TCM culture-based educational system,with seamless cooperation of all departments throughout the entire educational process and the integration of the five dimensions of education.Through the aforementioned practice,Zhejiang Chinese Medical University has effectively addressed issues such as the lack of a collaborative education among all departments and how TCM culture can promote the"integration of the five dimensions of education",providing a paradigm for Chinese medicine institutions to carry out TCM culture-based education.

[Objective]To explore the pathogenesis of prostate cancer and the prevention and treatment with moxibustion based on the theory of"excessive fire dispersing Qi,and mild fire generating Qi".[Methods]Based on the original text of The Inner Canon of Huangdi,the theoretical connotation of"excessive fire dispersing Qi,and mild fire generating Qi",and its relationship with the pathogenesis of prostate cancer and the reinforcing and reducing of moxibustion were investigated.The application of moxibustion reinforcing and reducing,guided by classical theory,is established in the prevention and treatment of prostate cancer,and illustrated with typical medical cases to substantiate the argument.[Results]The concept of"excessive fire dispersing Qi,and mild fire generating Qi"describes the impact of physiological and pathological fire on the body's genuine Qi.Mild fire is gentle in nature and helps to generate the body's genuine Qi,while excessive fire is intense and can consume the body's genuine Qi.Dampness,heat,blood stasis,and toxins,which are factors that induce prostate cancer,cancer toxins and western medical treatments such as surgery,radiotherapy and chemotherapy can all manifest as"excessive fire"pattern,consume the body's genuine Qi,which cause the course of prostate cancer to present in different stages,such as the initial formation of cancer toxins,deficiency of genuine Qi with toxin accumulation and Yang deficiency with cold stagnation.The moxibustion of reinforcing technique is relatively gentle and suitable in temperature,embodying the momentum of"mild fire",while the moxibustion of reducing technique is relatively intense and high in temperature to expel pathogens,similar to the fierce momentum of"excessive fire".Guiding the treatment of prostate cancer with moxibustion based on"excessive fire dispersing Qi",and"mild fire generating Qi"should focus on nourishing the mild fire and suppressing the excessive fire,using moxibustion of reinforcing to nourish the deficiency with"mild fire generating Qi",and using moxibustion of reducing to suppress cancer toxins and reduce the consumption of Qi dynamics,highlighting the therapeutic effect in disease prevention and treatment,as well as in improving the adverse reactions of western medical treatment.In the medical case mentioned,the patient suffered from urinary incontinence due to the damage to genuine Qi caused by surgery and the failure to consolidate and control it properly.The patient was comprehensively diagnosed with a deficiency of both the spleen and kidney.In the treatment,mild moxibustion was used to strengthen the spleen,warm the kidney,and replenish and consolidate Qi.Emphasis was also placed on the combination of moxibustion and herbal medicine.After two months of treatment,the symptoms were brought under stable control.[Conclusion]Under the guidance of the theory that"excessive fire dispersing Qi,while mild fire generating Qi",moxibustion techniques are flexibly selected in order to drain excess to suppress"excessive fire"and tonify deficiency to enhance"mild fire",which is well-suited to the complex pathogenesis of prostate cancer,which is characterized by a combination of deficiency and excess.This method provides an important reference for the prevention and treatment of the disease.

[Objective]To summarize SUN Simiao's clinical experience in treating vomiting and explore the modern application of SUN's method for treating vomiting,taking diabetic gastroparesis vomiting(DGPV)as an example.[Methods]By excavating and organizing the special chapters or paragraphs related to vomiting in SUN Simiao's works Emergency Thousand Gold Prescriptions and Thousand Gold Wing Prescriptions,it comprehensively analyzes SUN's understanding of the causes and treatment of vomiting,and applies it to clinical practice based on the pathogenesis of DGPV.[Results]Vomiting is a common clinical symptom of digestive tract abnormalities.Although conventional western medicine treatment can temporarily alleviate vomiting,it is prone to recurrence.SUN Simiao had profound insights in the diagnosis and treatment of vomiting,believing that"abnormal lifting and descending of the middle-Jiao,upward invasion of stomach Qi""cold,heat,phlegm,water,food and various evils invading the middle-Jiao"and"long-term illness injuring the five Zang organs and insufficient Qi and blood"were the core pathogenesis of vomiting.Through inductive analysis of his works,six methods and corresponding prescriptions can be extracted:harmonizing the stomach and descending Qi to stop vomiting,warming Yang and dispelling cold to stop vomiting,resolving phlegm and promoting diuresis to stop vomiting,digesting food and resolving stagnation to stop vomiting,clearing heat and nourishing fluids to stop vomiting,and regulating organs and tonifying deficiency to stop vomiting.In the clinical treatment of DGPV,small prescriptions and corner herbs for treating vomiting should be used in a targeted manner based on the core pathogenesis of different stages.[Conclusion]SUN Simiao's thoughts on treating vomiting still have certain application value in modern medicine and provide guidance for clinical treatment of vomiting such as DGPV.

[Objective]To summarize Professor ZHANG Guangji's thoughts and clinical experience in the treatment of senile insomnia based on the theory of"treating senile diseases from the perspective of Fu-organs".[Methods]By sorting out the discussions of previous medical scholars on the theory of"treating senile diseases from the perspective of Fu-organs"and Professor ZHANG's clinical interpretation,this paper,from three aspects,including the meaning of"treating senile diseases from the perspective of Fu-organs",the theoretical basis and specific application of treating senile insomnia from the perspective of Fu-organs,combining with typical medical records observed in the clinic,explores the feasibility and practical ideas of treating senile insomnia from the perspective of Fu-organs.[Results]Professor ZHANG believes that the meaning of Fu-organs includes not only the six hollow organs but also the source of engendering transformation,as well as the blood vessels and Xuanfu(sweat pore).The theoretical basis for treating senile insomnia by"seeking treatment from the Fu-organs"lies in the fact that the smoothness of the Fu-organs is of great significance for the sleep health of the elderly,and the obstruction of the Fu-organs plays an important role in the occurrence of senile insomnia.Specifically,the obstruction of the stomach,blood vessels and sweat pore impedes the pathway where Yin and Yang converge,resulting in their inability to interact.This disruption affects both the onset and maintenance of sleep.Therefore,in treatment,it is essential to focus on maintaining the smooth circulation of the gastrointestinal tract,blood vessels and sweat pore,enabling the free flow of Yin and Yang.A medical case was presented here.An elderly patient with insomnia was diagnosed with the syndromes of liver Qi stagnation transforming into fire,blood stasis obstructing the collaterals,stomach Qi disharmony and obstruction of the Xuanfu(minute orifices).By adopting the treatment principles of soothing the liver and regulating the stomach,promoting blood circulation to remove stasis and opening the Xuanfu,and calming the mind,good therapeutic effects were achieved.Specifically,Zhishi Xiaopi Pills were used to smooth the Qi circulation in the stomach-related Fu-organs,Xuefu Zhuyu Decoction was employed to promote blood circulation in the blood-related Fu-organs,and Danzhi Xiaoyao Powder combined with acupuncture was utilized to open the Xuanfu.[Conclusion]The theory of"treating senile diseases from the perspective of Fu-organs"is feasible,and Professor ZHANG Guangji's clinical experience is unique,providing a certain perspective for the prevention and treatment of senile insomnia.

[Objective]To analyze the effect of lever positioning manipulation(LPM)on the stress and strain of lumbar spine and intervertebral disc in different regions by using three-dimensional finite element technique,so as to reveal the biomechanical mechanism of manipulation.[Methods]A three-dimensional finite element model of the whole lumbar spine was constructed based on lumbar CT images,and the validity of the model was verified.The finite element model was used to analyze and compare the differences in the stress and strain of lumbar vertebrae and intervertebral discs by LPM at different manipulation points.[Results]The finite element model constructed indicates the point of manipulation is about 15 mm beside the L4 spinous process,the stress around the L4-5 intervertebral disc is large and the intermediate stress is small.The minimum strain point of the L4-5 intervertebral disc is about the lateral area of the intervertebral disc.The stress and strain of other intervertebral discs are similar to those of L4-5 intervertebral discs.The nucleus pulposus of L4-5 intervertebral disc move forward relatively to the lumbar spine;the stress under the lumbar pedicle is large,L4 has a certain degree of activity in the direction of extension,left bending and right rotation.Manipulation point is in the L4 spinous process,the stress around the L4-5 intervertebral disc is large and the intermediate stress is small.The minimum strain point of the L4-5 intervertebral disc is about the central area of the intervertebral disc.The stress and strain of other intervertebral discs are similar to those of L4-5 intervertebral discs.The nucleus pulposus of L4-5 intervertebral disc move forward relatively to the lumbar spine;the stress under the lumbar pedicle is large,and L4 has activity in the extension direction,and the activity in other directions is almost zero.[Conclusion]The LPM has the tendency to restore lumbar curvature and correct lumbar scoliosis and rotation.It can make the lumbar intervertebral disc move closer to the middle and make the nucleus pulposus move forward relatively to the posterior edge of the lumbar spine to reduce disc herniation and release compression.In the treatment of lumbar disc herniation,the manipulation points of the corresponding position can be selected according to the prominent position.

[Objective]To summarize Professor ZHANG Yonghua's academic experience in the treatment of panic disorder by using the theory of emotion-pattern differentiation.[Methods]Through follow-up study,reading ancient books and sorting out clinical medical cases,this paper analyzed Professor ZHANG Yonghua's clinical experience in exploring the etiology and pathogenesis of panic disorder from the perspective of emotion,as well as the clinical experience of using emotion-state syndrome differentiation theory in the treatment of panic disorder,accompanied with a medical case.[Results]The etiology of panic disorder is mainly related to the invasion of external evils,emotional stimulation and endowment differences,among which Professor ZHANG Yonghua believes that endowment personality is the basis of this disease.The pathogenesis is mainly related to fiery heat,phlegm obstruction,Qi depression,and lack of heart and Yang,but the first three are mainly mixed with each other to cause disease.According to the clinical characteristics and personality manifestations,panic disorder is divided into four categories:impatience and irritability,worry,sadness and depression,and simple panic.In terms of treatment,the combination of disease differentiation,syndrome differentiation and sentiment differentiation pays attention to flirting,and the treatment is mainly to calm the nerves,as well as clear heat,dissolve phlegm,regulate Qi and warm Yang.The proved case belonged to the type of impatience and irritability-more thinking and more consideration through emotional syndrome differentiation.The treatment was to clear away heat and phlegm,calm the nerves,administered with Chaihu plus Longgu Muli Decoction combined with Anshen Dingzhi Pill,and finally achieved good results.[Conclusion]Professor ZHANG Yonghua's treatment of panic disorder based on the theory of"emotion-state syndrome differentiation"has a significant curative effect,which is worthy of clinical promotion and learning.

[Objective]To summarize Professor LI Xinmin's clinical experience in treating childhood epilepsy by utilizing the method of strengthening the essence,removing phlegm,regulating Qi and awakening the mind.[Methods]By learning from the teacher,recording and organizing typical medical cases,the clinical experience of Professor LI in treating childhood epilepsy by using the method of"strengthening the essence,removing phlegm,regulating Qi and awakening the mind"was summarized and concluded from the aspects of etiology,pathogenesis,treatment methods and prescriptions,etc.A verified case was attached for corroboration.[Results]Professor LI believes that childhood epilepsy is based on the root cause of deficiency of the essential Qi and the manifestation of phlegm and Qi.He proposes that the disorder of Qi movement and the obstruction of the spirit with phlegm are the key to its pathogenesis,and clinically advocates the application of the method of strengthening the essence,removing phlegm,regulating Qi and awakening the mind.According to the characteristics of different epilepsy syndromes and combined with the classification of modern medicine,he summarizes and puts forward the following viewpoints:"treating generalized tonic-clonic seizures by strengthening the spleen,regulating Qi,eliminating phlegm and calming the wind""treating pediatric psychomotor epilepsy with the method of regulating Qi and eliminating phlegm""treating pediatric abdominal epilepsy by strengthening the spleen,removing phlegm,regulating Qi and harmonizing the middle""differentiating and treating pediatric epilepsy absence seizures from the perspectives of the liver and spleen".The attached verified case was an epileptic absence seizure,the syndrome differentiation belonged to the evil depression,and Shaoyang meridians and the gallbladder meridian Qi reversal,and the treatment adopted the law of evacuating the Shaoyang Qi and calming the nerves;the prescription was modified from Chaihu and Longgu Muli Decoction.[Conclusion]Professor LI's use of the method of strengthening the essence,removing phlegm,regulating Qi and awakening the mind in the treatment of childhood epilepsy has demonstrated certain clinical efficacy and possesses certain practical value,which is worthy of learning and reference.

[Objective]To explore the regarding of YANG Jizhou,the renowned expert in Zhejiang school of acupuncture and moxibustion,in facial aesthetic conditions and facial aging,as well as the acupuncture treatment strategies and methods,in order to provide guidance for clinical practice.[Methods]The study involves a review of YANG Jizhou's book Great Compendium of Acupuncture and Moxibustion,review relevant chapters and sections to conduct a comprehensive analysis from the perspectives of visceral differentiation,meridian differentiation,and YANG's acupuncture moxibustion technique.[Results]YANG proposed theories of facial regional correspondence with internal organs and facial chromatic diagnosis,providing guidance for the bidirectional diagnostic approach of"assessing internal conditions through external manifestations"and"pursuing beauty by addressing internal harmony"in acupuncture treatment of facial aesthetic impairments.YANG's meridian-based acupoint selection methodology further contributed numerous empirical acupoints and acupoint combinations to acupuncture cosmetology.In therapeutic approaches,YANG advocated the combined use of acupuncture,moxibustion,herbal medicine and massage,and further developed specialized techniques including skin needling,bloodletting,tendon needling,and muscle needling,along with penetration needling methods and moxibustion applications,which continue to exert significant influence on contemporary acupuncture cosmetic treatment techniques.[Conclusion]YANG Jizhou's diagnosis and treatment of facial aesthetic impairments and aging based on visceral-meridian pattern differentiation.He proposed a holistic aesthetic philosophy of"assessing internal conditions through external manifestations"and"pursuing beauty by addressing internal harmony",flexibly applied various acupuncture and moxibustion methods to treat skin,muscle,tendons and blood in layer.His rich expertise in diagnostic approaches,acupoint formulation strategies and specialized needling techniques are worthy exploring.