Objective To investigate the influencing factors of pregnancy outcomes after transcervical resection of polyp(TCRP)in infertility patients.Methods The data of 442 patients with infertility complicated with endometrial polyp(EP)underwent TCRP from March 2021 to March 2022 were retrospectively analyzed,and the postoperative follow-up time was 12-24 months,and they were divided into postoperative pregnancy group(pregnancy group)and non-pregnant group(control group).The two groups were compared with age,body mass index(BMI),infertility type,abnormal uterine bleeding,adverse pregnancy history,number of EP,EP size,intraoperative negative pressure uterine aspiration,endometritis,TCRP operation time,and uterine cavity depth,and multivariate analysis was conducted on the influencing factors of pregnancy outcomes after TCRP in infertility patients.Results Among 442 infertility patients with EP,274 cases(62.0%)were in the pregnancy group and 168 cases(38%)in the control group.The factors influencing pregnancy after TCRP were age(OR=0.925,95%CI:0.881-0.972,P<0.05),abnormal uterine bleeding before surgery(OR=0.646,95%CI:0.432-0.967,P<0.05)and primary infertility(OR=2.105,95%CI:1.295-3.423,P<0.05).Conclusion TCRP can improve pregnancy outcomes in infertility patients with EP.Age and abnormal uterine bleeding before operation were the protective factors to increase pregnancy after TCRP in infertility patients.Primary infertility is a risk factor for pregnancy after TCRP in infertility patients.

Objective To analyze the epidemic trend and characteristics of notifiable infectious diseases in Xindu District of Chengdu,and to explore the changes of disease spectrum,so as to provide scientific basis for formulating prevention and control strategies.Methods The epidemic data of notifiable infectious diseases in Xindu District of Chengdu from 2008 to 2022 were collected and analyzed by descriptive epidemiological method.Results From 2008 to 2022,a total of 66 302 cases of 28 kinds of notifiable infectious diseases were reported in Xindu District of Chengdu,with an average annual reported incidence of 516.94/100 000.A total of 266 deaths were reported,with an average annual mortality rate of 2.07/100 000 and a case fatality rate of 0.40% .No Class A infectious diseases were reported,and Class B infectious diseases had no obvious seasonality.Class C infectious diseases had two incidence peaks from June to August and from October to December.The sex ratio of male to female was 1.41∶1,and the age of onset was mostly 0-4 years old.The reported cases were mainly scattered children,preschool children,students,farmers,housework and unemployed people.According to the main mode of transmission,the annual average reported incidence rate from high to low was intestinal infectious diseases,respiratory infectious diseases,blood and sexually transmitted infectious diseases,insect-borne and natural focal infectious diseases,and other infectious diseases.The mode of transmission gradually transitioned from intestinal infectious diseases to respiratory infectious diseases.From 2020 to 2022,except for COVID-19 and influenza cases,the reported incidence of infectious diseases in other transmission routes decreased.The reported diseases were mainly concentrated in tuberculosis,hepatitis B,syphilis,mumps,hand-foot-mouth disease,influenza and other infectious diarrhea,and the annual reported incidence order changed slightly.Conclusion From 2008 to 2022,the reported incidence of Class B infectious diseases in Xindu District of Chengdu showed a steady decline,while the reported incidence of Class C infectious diseases showed a fluctuating upward trend.The prevention and control measures of the new coronavirus epidemic are conducive to reducing the incidence of notifiable infectious diseases.It is necessary to focus on the prevention and control of high-risk infectious diseases such as hand-foot-mouth disease,influenza,other infectious diarrhea,tuberculosis,hepatitis B,and acquired immune deficiency syndrome,with highly reported mortality.Targeted health interventions should be carried out for key populations,key seasons,and key areas.At the same time,the monitoring of new and rare infectious diseases should be strengthened.

Objective To investigate the prognostic effect of CD38 in patients with esophageal squamous cell carcinoma patients(ESCC).Methods A retrospective cohort study with 142 ESCC patients was performed.The clinicopathological features and expression of CD38,CD4,CD8,Ki-67,programmed cell death ligand 1(PD-L1)and programmed cell death 1(PD-1)in tumor and immune cells were evaluated.Results Among ESCC patients with lymph node metastasis,the expression of CD38 had a significant association with the overall survival(P=0.037).The median overall survival was 14 months and 38 months among patients with low and high expression of CD38,respectively.The crude and adjusted hazard ratio of high CD38 expression was 0.46(95% CI:0.21-0.98)and 0.32(95% CI:0.13-0.76)respectively.The expression of CD38 had a negative correlation with PD-L1 expressed in tumor cells.Conclusion The high expression of CD38 was associated with a better survival for ESCC with lymph node metastasis.

Performing multiple tests without adjusting the test level results in a higher-than-intended overall familywise error rate(FWER).This phenomenon is known as the multiplicity problem.In this paper,we first introduced the mechanism of multiplicity problem based on the classification and characterization of clinical endpoints.Then,strategies and methods to solve the multiplicity problem were introduced,including the parallel strategy/single-step method,the sequential strategy/multistep method/stepwise method,and their combinations.The results of different analytic strategies may vary.The practical application of the above common strategies and statistical methods were introduced through the case studies of domestic and foreign investigator initiated clinical trial.Multiplicity adjustment for multiple endpoints in clinical trials can be achieved by a single strategy or a combination of strategies.Depending on the selected strategy or combination,the statistical methods and significance level(denoted as α)for each test hypothesis are determined to effectively control the multiplicity problem.

Objective This paper aims to summarize the core competencies that editors of scientific journals should possess and propose improvement strategies to promote the cultivation of editors of scientific journals to meet the needs of construction and cultivation of world-class scientific journals in China.Methods The role,responsibilities,and qualifications of editors of top international academic journals were surveyed.Questionnaire surveys were conducted among researchers with academic publication experience,and interviews were held with experts in medicine,journalism,and publishing industry.The core competencies that editors of scientific journals should possess were analyzed.Results The findings indicate that editors of scientific journals need to possess professional academic abilities,including keen academic insight and disciplinary expertise;editorial skills,including proficiency in norms and standards of academic journal and publishing processes,and the ability to learn promptly new knowledge related to publishing to meet the needs of modern academic publishing;and publishing service capabilities,including academic organizational capabilities and communication skills,as well as interpersonal communication skills.The ability to establish,maintain,and develop a network of interpersonal relationships is also crucial.Improvement strategies mainly include building an industry environment for talent growth,where the editor determines the goals and directions through their own efforts.Conclusion The core competencies of editors of scientific journals can be enhanced through building an industry environment for talent growth and the efforts of editors themselves,making editors of scientific journals interdisciplinary talents and promoting the rapid and high-quality development of domestic scientific journals.

Objective To investigate the role and mechanism of AMP-activated protein kinase(AMPK)in regulating mitochondrial function and cardiomyocyte injury in sepsis.Methods Forty Balb/c mice were randomly divided into four groups:Sham group(n=10),Sham+5-aminoimidazole-4-carboxamide ribonucleotide(AICAR)group(n=10),CLP group(n=10),and CLP+AICAR group(n=10).A sepsis mouse model was established through cecal ligation and puncture(CLP).Echocardiography and histological analysis were used to assess sepsis-induced cardiac injury.Neonatal rat cardiomyocytes(NRCMs)were incubated with lipopolysaccharide(LPS)(10 μg/mL)for 24 h to induce an in vitro sepsis model,and treated with AICAR.Mitochondrial function and dynamics were assessed by using Western blotting,enzyme-linked immunosorbent assay(ELISA),and immunofluorescence assays.Results Compared with the Sham group,AMPK expression in the myocardial tissue of CLP mice was significantly reduced(P<0.05).Compared with the CLP group,AMPK expression in the CLP+AICAR group was significantly increased(P<0.05).Survival analysis showed that CLP led to a high mortality rate(～60％ ),while AICAR treatment significantly improved the survival rate of CLP mice(P<0.05).Compared with the Sham group,cardiac output(CO),stroke volume(SV),and left ventricular end-diastolic volume(LVEDV)were significantly decreased in the CLP group(P<0.05),while left ventricular posterior wall systolic(LVPWs)and left ventricular posterior wall thickness(LVPWd)were significantly increased(P<0.05).AICAR treatment alleviated the cardiac dysfunction induced by CLP.Compared with the CLP group,mitochondrial size and the number of mitochondrial cristae in the myocardial tissue of CLP+AICAR group mice were significantly increased(P<0.05),while DHE fluorescence intensity and the number of TUNEL-positive cells were significantly reduced(P<0.05).Compared with the LPS group,ATP production,mitochondrial respiration rate,and complex Ⅰ,Ⅱ,and Ⅲ activities in NRCMs of the LPS+AICAR group were significantly increased(P<0.05),while mitochondrial and cytoplasmic ROS levels were significantly reduced(P<0.05).Compared with the control group,mitochondrial size in NRCMs of the LPS group was significantly reduced(P<0.05),while Bax and Caspase-3 expression,as well as mitochondrial fission index,were significantly increased(P<0.05),and these changes were mitigated by AICAR(P<0.05).Conclusion AMPK plays a crucial role in maintaining cardiac function and mitigating septic myocardial injury by regulating mitochondrial structure and function,energy metabolism,oxidative stress,and apoptosis.

Objective To study the infiltration of neutrophils and macrophages in the tissues of eosinophilic gastroenteritis mice.Methods Thirty female C57BL/6 mice aged 6-8 weeks were selected to induce the model of eosinophilic gastroenteritis in wild-type mice.They were sensitized by intraperitoneal injection of ovalbumin(OVA)protein and they were gastric stimulated with OVA.The successful modeling of eosinophilic gastroenteritis was verified by measuring the weight change of mice,detecting OVA specific immunoglobulin E(IgE)and Immunoglobulin G(IgG)levels in peripheral blood of mice by enzyme-linked immunosorbent assay(ELISA),and detecting intestinal eosinophils count by flow cytometry.The proportion of neutrophils and monocytes in peripheral blood and the proportion of neutrophils and macrophages in spleen,mesenteric lymph nodes,small intestine and colon tissues were detected by flow cytometry.Results The mice model of eosinophilic gastroenteritis was successfully induced by OVA abdominal sensitization and gavage stimulation.In mice with eosinophilic gastroenteritis,the infiltration of monocytes in peripheral blood,macrophages infiltration of spleen,mesenteric lymph nodes,small intestine and colon tissues were increased.And the neutrophil were increased infiltrated in peripheral blood,small intestine and colon tissues.Conclusion Macrophage and neutrophil were increased infiltrated in peripheral blood,intestinal tissue of mice with eosinophilic gastroenteritis.Furthermore,the infiltration of macrophage was increased in lymphoid tissues,such as spleen and mesenteric lymph nodes of mice with eosinophilic gastroenteritis.

Objective To explore the correlation between serum hypoxia-inducible factor-1α(HIF-1α)levels and cerebral microbleeds(CMBs)and cognitive impairment and to assess the predictive value of HIF-1α for CSVD-related cognitive impairment.Methods A total of 104 patients with CSVD who attended the Department of Neurology,First Affiliated Hospital of Xinxiang Medical University from June 2022 to November 2023 were enrolled.All enrolled patients were subjected to basic statistics,cranial nuclear magnetic resonance examination,cognitive function assessment,and serum HIF-1α test,and the number and location of CMBs were counted.Based on the above data the enrolled patients were grouped.The correlation between HIF-1α and cognitive function and CMBs was studied the influencing factors of CMBs and cognitive impairment were analyzed,and the predictive value of HIF-1α on the occurrence of cognitive impairment was evaluated.Results There were statistically significant differences in HIF-1α levels and cognitive function among different CMBs groups.Serum HIF-1α levels were significantly negatively correlated with overall cognitive function,visuospatial and executive function,attention,and delayed recall,and serum HIF-1α was positively correlated with the number of CMBs.HIF-1α may be a risk factor for CMBs and cognitive impairment associated with CSVD,and serum HIF-1α has potential in predict the cognitive impairment caused by CSVD.Conclusion Serum levels of HIF-1α were associated with the number of CMB and CSVD-related cognitive impairment,and serum levels of HIF-1α may have a predictive value for CSVD-related cognitive impairment.

Objective To investigate the effect of large artery atherosclerosis(LAA)and non-LAA subtypes on the efficacy of Ginkgo Diterpene Lactone Meglumine(GDLM)in patients with acute ischemic stroke.Methods This was a post-hoc analysis of multicenter,randomized,double-blind,placebo-controlled,and parallel-group trial.A total of 3 448 patients who had acute ischemic stroke were randomly assigned in a 1∶1 ratio to receive the injection of GDLM or the placebo once day within 48 h after symptoms and continued for 14 d.The primary outcome was the proportion of patients with a modified Rankin Scale(mRS)of 0 or 1 on day 90 after randomization.Results A total of 3 448 patients were enrolled,with 1 604(46.52% )patients with non-LAA and 1 844(53.48% )with LAA.Compared to the placebo treatment.GDLM injection effectively improve the functional prognosis,with a higher proportion of mRS score of 0-1 in both non-LAA(OR=1.24,95% CI:1.02-1.51;P=0.03)and LAA(OR=1.37,95% CI:1.14-1.65;P<0.001)group.There was no significant interaction between LAA subtypes with treatment(P=0.48 for interaction).Conclusion Among patients with acute ischemic stroke in this randomized clinical trial,GDLM might improve the favorable clinical outcomes at 90 d compared with placebo,regardless of LAA subtypes.Nevertheless,it is necessary to confirm the findings in the future.

The"promising zone"is a method used to analyze interim data from adaptive design clinical trials in an unblinded state.It allows for the adjustment of sample size based on interim results to enhance the trial's probability of success or minimize investment in unnecessary sample size.Mehta and Pocock proposed rules for increasing sample size based on interim analysis results using the concept of the"promising zone"(MP design).Furthermore,combination of the MP design with group sequential design can set up early stopping boundaries in trials,allowing for a reduction in sample size under favorable or unfavorable zone.The combination test(CT)design further optimizes the framework of the"promising zone",by considering sample size and conditional power in combination to achieve the highest conditional power with the smallest sample size.This review summarizes the principles of the"promising zone",introduces the method of determining the"promising zone"and re-estimating sample size,and further illustrates the feasibility of this method in clinical trials with a practical case.