Objectives: Possible sources of drugs need to undergo stringent testing before any clinical trials are undertaken. Vital to this is the testing of these drugs on animals. The need for a homogeneous animal xenotype to humans is not only necessary to understand biologic genetic factors that influence the phenotypic characteristics of the disease process but also serves as a basis for developing rational interventional strategies. This research aims to explore the BLCA-4 gene sequence of MNU induced urinary bladder cancer in rats and urinary bladder cancer in humans. Materials and Methods: Transitional cell carcinoma was induced in 3 male, age matched Wistar rats through intravesical instillation of 0.2 ml of N-Methyl-N-nitrosourea and were sacrificed after 21 days. The urinary bladders of the rats were collected. Portions were sent for histopathologic studies by a veterinarian pathologist and the mucosa was scraped from the remaining tissues for further testing. Samples of 1.0 cm x 1.0 cm of human bladder tissue were also collected during the process of transurethral resection of bladder tumor with its mucosa scraped off for further testing. The genomic DNA from the colected urinary bladder mucosa were isolated using the WizardÃ
Bladder Carcinoma ; Gene Sequence