OBJECTIVE To analyze the relationship between interleukin-35(IL-35)and serine/arginine-rich splicing factor 6(SRSF6)levels and pleural thickness in patients with tuberculous pleurisy,as well as their values of joint assessment for the risk of pleural tuberculoma.METHODS A total of 100 patients with tuberculous pleurisy admit-ted to the Second Affiliated Hospital of Zhejiang University School of Medicine,Linping Campus,from Jan.2022 to Jul.2024 were selected.The IL-35 and SRSF6 levels in pleural effusion and pleural thickness were measured.The relationship between IL-35,SRSF6 levels and pleural thickness were analyzed.After a one-year follow-up,patients with tuberculosis were divided into the occurrence group and non-occurrence group according to whether pleurotuberculoma developed.The risk factors for developing pleurotuberculoma and the value of IL-35 and SRSF6 in accessing the risk of developing pleurotuberculoma were analyzed.RESULTS In patients with tuberculous pleuri-sy,IL-35 and SRSF6 in pleural effusion was(529.78±146.85)ng/L and(1.44±0.43)ng/ml,respectively,and the pleural thickness was(4.07±0.53)mm.IL-35 and SRSF6 levels in pleural effusion were positively correlated with pleural thickness(P<0.05).Logistic analysis showed that the risk of pleural tuberculoma significantly in-creased in patients with tuberculous pleurisy when there was pleural fluid separation,abnormal pleural thickness and elevated levels of IL-35 and SRSF6 in pleural effusion(P<0.05).Receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC),specificity and sensitivity of combined IL-35 and SRSF6 in predicting the occurrence of pleural tuberculoma in patients with tuberculous pleurisy were significantly higher than those of either marker alone(P<0.05).CONCLUSION IL-35 and SRSF6 levels are positively correla-ted with the pleural thickness of tuberculous pleurisy,and the combined detection of IL-35 and SRSF6 can effec-tively assess the risk of pleural tuberculoma in tuberculous pleurisy patients.

OBJECTIVE To analyze the relationship between interleukin-35(IL-35)and serine/arginine-rich splicing factor 6(SRSF6)levels and pleural thickness in patients with tuberculous pleurisy,as well as their values of joint assessment for the risk of pleural tuberculoma.METHODS A total of 100 patients with tuberculous pleurisy admit-ted to the Second Affiliated Hospital of Zhejiang University School of Medicine,Linping Campus,from Jan.2022 to Jul.2024 were selected.The IL-35 and SRSF6 levels in pleural effusion and pleural thickness were measured.The relationship between IL-35,SRSF6 levels and pleural thickness were analyzed.After a one-year follow-up,patients with tuberculosis were divided into the occurrence group and non-occurrence group according to whether pleurotuberculoma developed.The risk factors for developing pleurotuberculoma and the value of IL-35 and SRSF6 in accessing the risk of developing pleurotuberculoma were analyzed.RESULTS In patients with tuberculous pleuri-sy,IL-35 and SRSF6 in pleural effusion was(529.78±146.85)ng/L and(1.44±0.43)ng/ml,respectively,and the pleural thickness was(4.07±0.53)mm.IL-35 and SRSF6 levels in pleural effusion were positively correlated with pleural thickness(P<0.05).Logistic analysis showed that the risk of pleural tuberculoma significantly in-creased in patients with tuberculous pleurisy when there was pleural fluid separation,abnormal pleural thickness and elevated levels of IL-35 and SRSF6 in pleural effusion(P<0.05).Receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC),specificity and sensitivity of combined IL-35 and SRSF6 in predicting the occurrence of pleural tuberculoma in patients with tuberculous pleurisy were significantly higher than those of either marker alone(P<0.05).CONCLUSION IL-35 and SRSF6 levels are positively correla-ted with the pleural thickness of tuberculous pleurisy,and the combined detection of IL-35 and SRSF6 can effec-tively assess the risk of pleural tuberculoma in tuberculous pleurisy patients.

Objective:To observe the effect of early temperature control on the prognosis of brain injury patients after severe carbon monoxide poisoning (COP).Methods:A total of 277 patients hospitalized with severe COP were randomly divided into a fever group ( n=78), a normal temperature group ( n=113) and a mild hypothermia group ( n=86). All were given hyperbaric oxygen therapy and any necessary supportive treatment. The mild hypothermia group were kept in a room at 34 to 35℃. Evaluation was with the Glasgow Coma Scale (GCS), version II of the Acute Physiology and Chronic Health Evaluation (APACHE), the Hasegawa dementia scale (HDS) and the mini mental state examination (MMSE). The incidence of delayed encephalopathy (DEACMP) and mortality were compared among the three groups. The bispectral index (BIS) and neuron-specific enolase (NSE) levels were correlated with DEACMP. Results:After the treatments, improvement was observed in multiple indexes of all three groups compared with before the treatment. Compared with the fever group, the average GCS of the mild hypothermia group was significantly higher on the 2nd, 4th, 8th and 31st day after the intervention. It was significantly higher than the normal temperature group′s averages on the 4th, 8th and 31st day. The average APACHE scores of the normal temperature and the mild hypothermia groups were significantly lower than the fever group′s average, with that of the mild hypothermia group significantly lower than that of the normal group. The average HDS scores of the normal temperature and mild hypothermia groups were significantly higher than the fever group′s average, with that of the mild hypothermia group significantly higher than that of the normal group. The average MMSE score of the mild hypothermia group was significantly improved after 7 days, one month and three months of treatment. That of the normal group showed significant improvement after one and three months, but the mild hypothermia group′s averages were superior. Compared with the fever group, the average BIS score of the mild hypothermia group was significantly better after one, three and seven days, and one month. This was true for the normal group beyond three days after the intervention. The average NSE concentration of the normal group after 7 days and one month was significantly lower than that of the fever group. For the mild hypothermia group this was true after only 3 days. Compared with the other two groups, the average coma time, incidence of DEACMP and nervous system injury were significantly lower in the hypothermia group. The average GCS, BIS and NSE values were closely related to the occurrence of DEACMP.Conclusions:Early temperature control can significantly reduce the severity of brain injury after COP and reduce the incidence of neurological sequelae. Early dynamic detection of GCS, NSE concentration and BIS is of great significance for predicting the incidence of DEACMP.

Objective:To investigate the clinical values of laboratory inflammation indicators including lymphocyte count, C reactive protein (CRP), serum amyloid A protein (SAA), and procalcitonin (PCT) in the diagnosis and treatment of 2019 novel corona virus disease (COVID-19) patients.Methods:The data of clinical characteristics of 77 hospitalized COVID-19 patients were collected from February to March 2020. According to the guidelines for diagnosis and management of COVID-19, these patients were divided into moderate type (49 cases) and severe type (28 cases). Among them 19 cases were discharged. The differences of lymphocyte count, CRP, SAA and PCT among moderate, severe and discharged groups were compared. The indicators with better predictive value for disease development and prognosis were screened out by logistic regression and receiver operating characteristic curve (ROC) analysis.Results:The severe patients had lower lymphocyte count compared to the moderate patients ( P=0.000), while their CRP ( P=0.000), SAA ( P=0.000) and PCT positive rate ( χ2=11.003, P=0.001) were significantly higher; The lymphocyte count of discharged patients was much higher than that of moderate ( P=0.001) and severe ( P=0.000) patients, while CRP ( P=0.036, P=0.000) and SAA ( P=0.002, P=0.000) were dramatically lower; Although the discharged patients had much lower PCT positive rate than severe patients ( χ2=6.891, P=0.009), they were not significantly different from moderate patients ( χ2=0.169, P=0.681). Logistic regression analysis showed that the increased SAA and CRP were both risk factors for predicting severe illness, but the decreased SAA were demonstrated as an independent indicator for discharge. The ROC curve analysis showed that the sensitivity and specificity of SAA for diagnosis of severe patients were 85.71% and 87.76%, whereas CRP were 82.14% and 93.88%. Further, the sensitivity and specificity of SAA for predicting discharge were 100% and 57.89%. Conclusions:The laboratory inflammation indicators such as lymphocyte count, CRP, SAA and PCT had important clinical significance for the diagnosis and treatment of COVID-19 patients. Among them, SAA was demonstrated as an independent indicator with high sensitivity for evaluating the disease progression and prognosis.

Objective To study the irrational intravenous (IV) medication orders and promote rational medication applications.Methods A retrospective analysis was conducted to analyze the irrational prescriptions from January to December 2016.Results Among 39 948 IV medication orders reviewed, there were 134 irrational prescriptions of cytotoxic drugs (0.33％) and 222 irrational prescriptions of total parenteral nutrition(TPN)(0.56％).Within 356 irrational prescriptions, 19 had improper amount of diluent(5.34％), 95 inappropriate dosage(26.69％), 12 drug incompatibility(3.37％), 8 wrong diluent (2.24％) and 222 inappropriate TPN orders (62.36％).Conclusion The interventions of the clinical pharmacists are needed to reduce medication errors and improve the drug safety and effectiveness.

Objective To build the index about the training effect of oncology specialist nurse and test the reliability and validity.Methods By consulting literature and the methods of Delphi,which was consulted by the 9 experts and the two rounds of 35 experts,the original index was estabhshed.The correlation coefficient and validity of the index was evaluated.Results A total of the index was consist of 106 first-l,second-land third-llevel items.Questionnaire recovery rate was 100％ (35/35).The level of authority was 0.816.Expert opinion concentration in various purposes mean variation coefficient was less than 0.244 2.The coordination level of expertise was 0.302.The overall reliability Cronbach α was 0.963.Adopting the method of double variable Spearman calculated the correlation coefficient was above 0.428.Cronbach a of second-and third-level indexes were 0.512-0.895.The second-land third-level indexes correlation coefficient were 0.428-0.931.Conclusions The index about the training effect of oncology specialist nurse has the high authority and representative.The reliability and validity of the index are better,which can be used to evaluate the efficacy of the evaluation about the training of oncology specialist nurse.

microRNAs (miRNAs) are an extensive class of -22-nucleotide (nt) endogenous noncoding RNAs regulating life activities ofmetazoans through binding to 3'-untranslated regions (3'-UTRs) of their target genes. This work aimed to identify yan gene in the silkworm, reveal its expression profile and confirm if it is one target of bmo-miR-7 and, as such, have potential for contributing to better understanding of the molecular mechanisms involved in the metamorphosis of silkworm. Based on homolog searching and PCR amplification, we cloned the coding sequence (CDS) of Bmyan, which encodes 476 amino acid residues and contains SAM-PNT and ETs domains. Quantitative PCR (q-PCR), RT-PCR and microarray data revealed high expression of Bmyan in the head, body wall and ovary of day-3 fifth instar larval silkworm, low or no expression in other tissues. It was lowly expressed in the early larval stages, but highly expressed from late spinning to day 4 pupa. The 3'-UTR of Bmyan was obtained by rapid-amplification of cDNA ends (3'RACE) and predicted to contain two potential recognition sites of bmo-miR-7. The luciferase reporter vector containing the 3'-UTR of Bmyan was constructed and co-transfected into BmE cell line with the mimic of bmo-miR-7 and the decreased relative activity of luciferase showed that Bmyan is one target of bmo-miR-7. This work helps further functional analysis of bmo-miR-7 and Bmyan in the silkworm.
3' Untranslated Regions ; Animals ; Bombyx ; genetics ; Cell Line ; Cloning, Molecular ; Female ; Genetic Vectors ; Insect Proteins ; genetics ; Larva ; Metamorphosis, Biological ; MicroRNAs ; genetics ; Pupa