This paper analyzes the characteristics of highly cited papers published in Chinese Clinical Pharmacology and Therapeutics in the past ten years,in order to explore the influence and re-search value of these papers.Highly cited papers are an important index to evaluate the scientific re-search level of scholars and research institutions,so this study focuses on the characteristics of their number,citation frequency,number of co-authors and regional distribution.The paper uses bibliomet-rics to analyze the publication trend and citations of highly cited papers.The results show that during the observation period,the number of highly cited papers decreases year by year,and there is a signifi-cant correlation between cited frequency and publi-cation time.In addition,the number of co-authors presents a normal distribution,among which the number of co-authors is the largest,indicating that moderate cooperation has a positive effect on the influence of papers.From the perspective of geo-graphical distribution,the author group of the mag-azine is relatively dispersed,covering many regions of the country,among which Anhui,Hunan and Zhejiang have become the main sources of high-quality manuscripts.The finding reflects the maga-zine's wide reach across the country.The results of this study provide empirical support for under-standing the characteristics of highly cited papers and emphasize the importance of research collabo-ration and geographical factors in research publica-tion.Through the analysis of highly cited papers,this paper provides references for subsequent re-searchers in selecting research topics and coopera-tion methods,and also provides beneficial sugges-tions for improving the research level and influence of clinical pharmacology in China.

This paper analyzes the characteristics of highly cited papers published in Chinese Clinical Pharmacology and Therapeutics in the past ten years,in order to explore the influence and re-search value of these papers.Highly cited papers are an important index to evaluate the scientific re-search level of scholars and research institutions,so this study focuses on the characteristics of their number,citation frequency,number of co-authors and regional distribution.The paper uses bibliomet-rics to analyze the publication trend and citations of highly cited papers.The results show that during the observation period,the number of highly cited papers decreases year by year,and there is a signifi-cant correlation between cited frequency and publi-cation time.In addition,the number of co-authors presents a normal distribution,among which the number of co-authors is the largest,indicating that moderate cooperation has a positive effect on the influence of papers.From the perspective of geo-graphical distribution,the author group of the mag-azine is relatively dispersed,covering many regions of the country,among which Anhui,Hunan and Zhejiang have become the main sources of high-quality manuscripts.The finding reflects the maga-zine's wide reach across the country.The results of this study provide empirical support for under-standing the characteristics of highly cited papers and emphasize the importance of research collabo-ration and geographical factors in research publica-tion.Through the analysis of highly cited papers,this paper provides references for subsequent re-searchers in selecting research topics and coopera-tion methods,and also provides beneficial sugges-tions for improving the research level and influence of clinical pharmacology in China.

AIM: To study chronopharmacokinetics of helicid and its metabolites. METHODS: An HPLC-MS method for simultaneous determination of helicid and its three phase I metabolites were established and validated. At 8:00, 14:00 and 0:00, the rats were given helicid 50 mg/kg by gavage, respectively. Blood samples were collected from ophthalmic venous plexus. Then plasma concentration was measured. Pharmacokinetic behaviors of the original drug and its metabolites after administration at different time points were calculated and compared. RESULTS: This established HPLC-MS/MS method was successfully applied to simultaneous determination of helicid and its three metabolites in rat plasma after intragastric administration. Using AUC

AIM: To investigate the absorption of helicid in different segments of intestine based on rat everted intestine sac model. METHODS: To establish a high-performance liquid chromatography method for simultaneous determination of helicid and its metabolite. Krebs-ringer solution containing helicid was added to everted intestine sacs of different segments (duodenum, Jejunum, ileum and colon). Drug concentration in sacs was determined at different time points (5, 10, 15, 30, 45, 60, 75, 90 min). Adsorptions of helicid in four intestinal segments were compared. RESULTS: This high-performance liquid chromatography was successfully applied to the simultaneous determination of helicid and its metabolite. Absorption of helicid was rapid and time-dependent. The absorption and metabolism of helicid in duodenum segment were higher than these in other segments. CONCLUSION: The duodenum segment is the main site of segmental absorption and metabolism of helicid. This is the first report on intestinal segment metabolism of helicid.