Objective To investigating the microbial communities and physicochemical properties of soil and distribution of Oncomelania hupensis snails in marshlands along the Yangtze River basin at different types of land use, and to examine the effects of soil microorganisms and physicochemical properties on snail distribution, so as to provide insights into snail control and schistosomiasis prevention in marshland along the Yangtze River basin. Methods Marshlands with four types of land use were selected along the Yangtze River basin on April 2021, including poplar forest-crops integrated planting, reed areas, agricultural cultivation lands and ditches. The distribution of snails and physicochemical properties of soil were investigated in marshlands with different types of land use, and the V3 to V4 regions of the bacterial 16S ribosomal RNA (16S rRNA) gene, fungal internal transcribed spacer-1 (ITS1) gene and algal ribulose-bisphosphate carboxylase (rbcL) gene in soils were subjected to high-throughput sequencing. The occurrence of frames with living snails and density of living snails were compared in marshland with different types of land use. The associations of soil microorganisms and physicochemical properties with the density of living snails were examined using Pearson correlation analysis, and the contributions of soil microorganisms and physicochemical properties to the density of living snails were evaluated using variance partitioning analysis. Results In marshlands with four types of land use, the greatest occurrence of frames with living snails [(4.94 ± 2.14)%] and density of living snails [(0.070 ± 0.026) snails/0.1 m²] were seen in ditches, and the lowest were found in [(1.23 ± 1.23)%] agricultural cultivation lands [(0.016 ± 0.019) snails/0.1 m²]. A total of 2 phyla, 5 classes, 8 orders, 9 families and 11 genera of algae were detected in soils at four types of land use, with Chlorophyta as the dominant phylum and Pseudoneochloris as the dominant genus. A total of 44 phyla, 134 classes, 281 orders, 338 families and 516 genera of bacteria were detected in soils at four types of land use, with Proteobacteria and Acidobacteriota as the dominant phyla and uncultured Acidobacterium, MND1, Mitrospira, Haliangium and Sphingomonas as dominant genera. A total of 11 phyla, 41 classes, 108 orders, 223 families and 408 genera of fungi were detected in soils at four types of land use, with phyla Ascomycota, Basidiomycota and Mortierellomycota presenting high relative abundances and genera Cladorrhinum, Mortierella and Humicola presenting high relative abundances. Pearson correlation analysis revealed that the density of living snails correlated negatively with the relative abundance of Proteobacteria (r = −0.965, P < 0.05) and soil electronic conductivity (r = −0.962, P < 0.05) and positively with soil moisture (r = 0.951, P < 0.05). Variance partitioning analysis demonstrated that the physicochemical properties and microorganisms of soil contributed 69% and 10% to the density of living snails, respectively. Conclusion The diversity of microbial communities varies in soils at different types of land use in marshland along the Yangtze River basin, and the physicochemical properties and microorganisms of soils may affect the distribution of O. hupensis snails.

Objective:To understand molecular characteristics and antibiotic resistance of Clostridioides ( C.) difficile isolated from children in China, and provide data support the development of disease risk assessment and burden studies. Methods:A total of 155 strains of C. difficile isolated from children aged <12 years in 14 provinces (autonomous regions, municipalities) in China from 2010 to 2023 were used for the analyses on molecular characteristics and antibiotic resistance of C. difficile by PCR and drug susceptibility test. Results:A total of 26 sequence types (STs) and 18 ribotypes (RTs) were identified in the 155 C. difficile isolates, in which ST3 (20.65%), ST54 (16.13%), ST35 (12.90%), and RT012/ICDC007 (14.84%), RT001/ICDC001 (11.61%), RT046/ICDC018 (8.39%) were the most common. One highly virulent strain with RT078 and 27 non-toxin-producing strains were also found; the predominant toxin gene was tcdA+ tcdB+ cdt-. All the strains were sensitive to metronidazole and vancomycin, and there were 29 multidrug-resistant strains, in which 1 strain was resistant to all the seven antibiotics except for vancomycin and metronidazole. Conclusions:Molecular characteristics and antibiotic resistance of C. difficile in children were similar to those in whole population in China, but there were regional distribution differences. It is necessary to strengthen the routine drug-resistance surveillance for C. difficile infection in children in China.

Objective:To achieve the effect of EBV enrichment from different anticoagulants, a novel recombinant protein F was designed and produced.Methods:A novel recombinant protein F was designed, which contained human IL-2 signal peptide, his, human IgG1 Fc, human Ficolin-2 neck region and Fibrinogen-like domain (FGD). The synthetic gene segment was cloned into pCDNA?3.1(+ ) vector, which was then transfected into HEK293F cells. The recombinant protein (F protein) was then produced in supernatant. F beads was constructed by coating F protein onto protein A beads. At last, EBV positive whole blood samples anticoagulated by three kinds of anticoagulants were collected. F beads and M beads (previously studied) were compared to be applied to enrich EBV from these blood samples.Results:M beads were effective to enrich EBV from heparin or sodium citrate anticoagulated blood ( P<0.01), not for EDTA anticoagulated blood. While, F beads were effective for all three anticoagulated blood ( P<0.01). Conclusions:F beads could enrich EBV from different anticoagulated blood.

Objective:To explore the taste alterations and nutritional status of lung cancer patients during chemotherapy, and analyze the correlation between the two.Methods:From June to December 2021, continuous fixed-point sampling was used to select lung cancer inpatients receiving chemotherapy in the Department of Thoracic Medicine of Beijing Cancer Hospital, Peking University. The patients' taste alterations were evaluated using the Chemotherapy-induced Taste Alteration Scale (CiTAS), and their nutritional status was evaluated using the Nutritional Risk Screening 2002 (NRS 2002) and the Global Leadership Initiative on Malnutrition (GLIM). The patients' weight and calf circumference were also measured. Taste alterations were evaluated on the first day before chemotherapy and the eighth days (T1 to T8) of the first to fourth cycles, respectively, at a total of 8 time points. The nutritional status was evaluated at four time points, including T1, T3, T5, and T7. The correlation between taste alterations and nutritional status was analyzed using generalized estimation equations.Results:A total of 77 patients completed all evaluations. The total CiTAS scores of lung cancer patients from T1 to T8 were (4.21±0.05), (5.52±0.14), (4.67±0.08), (6.05±0.17), (4.84±0.10), (6.45±0.18), (4.97±0.10) and (6.57±0.16), respectively. At T1, T3, T5, and T7, the proportion of patients with positive nutritional risk was 10.4% (8/77), 22.1% (17/77), 26.0% (20/77) and 29.9% (23/77), respectively, and the body weight was (67.08±1.07), (66.46±1.09), (66.12±1.09), (65.88±1.09) kg, and the calf circumference was (35.00±2.31), (34.71±2.41), (34.49±2.45), (34.38±2.47) cm and the number of patients with malnutrition was 1 (1.3%), 6 (7.8%), 10 (11.3%) and 11 (11.3%), respectively. The total score of CiTAS was positively correlated with NRS 2002 score, malnutrition ratio, and weight loss, while negatively correlated with calf circumference, with statistically significant differences ( P<0.05) . Conclusions:Patients with lung cancer undergoing chemotherapy have significant taste alterations, which worsen after each cycle of chemotherapy and recover before the next cycle of chemotherapy, but cannot return to baseline levels. The nutritional status of lung cancer patients is deteriorating, with an increasing proportion of patients at nutritional risk and malnutrition, as well as a continuous decline in weight and calf circumference. Taste alterations affect the nutritional status of patients. It is suggested that attention should be paid to the management of taste alterations and nutritional intervention during chemotherapy to improve the nutritional status of patients.

Clostridioides difficile infection (CDI) is an infectious disease with fever, abdominal pain and diarrhea as the main clinical manifestations. At present, CDI is mainly treated with antibiotics and faecal microbiota transplantation. As recurrent and refractory CDI continues to increase, it is important to seek a more effective alternative therapy. However, many of the studies on the prevention and control of CDI by probiotics are still in the early stage. This paper summarized the research on the types, mechanisms and technical means of probiotics in the treatment of CDI.

Objective:To explore the mediating effect of medical coping style on psychological resilience and death anxiety in cancer patients.Methods:Using the convenient sampling method, a total of 330 inpatients from 2 cancer hospitals in Beijing were selected as the research objects from June to August 2020. The general information questionnaire, Chinese Version of Templer 's Death Anxiety Scale (C-T-DAS) , Medical Coping Modes Questionnaire (MCMQ) and the Connor-Davidson Resilience Scale (CD-RISC) were used to investigate them. Pearson correlation analysis and multiple linear regression were used to analyze the relationship between death anxiety, medical coping style and psychological resilience in cancer patients. AMOS 21.0 was used to establish the structural equation model and verify the mediation effect. A total of 330 questionnaires were sent out in this study, and 302 were effectively received, with the effective recovery of 91.5%. Results:The score of Chinese Version of Templer's Death Anxiety Scale for 302 cancer patients was (40.12±10.23) , the score of Connor-Davidson Resilience Scale was (70.97±13.43) and the scores of confrontation, avoidance and resighation dimension of Medical Coping Modes Questionnaire were (18.80 ±3.65) , (15.64±3.16) and (8.75±2.84) . The psychological resilience of cancer patients was negatively correlated with death anxiety and the resignation dimension in medical coping styles ( P<0.01) , and positively correlated with the facing and avoiding dimensions in medical coping styles ( P<0.05) . The face and yield dimensions in the medical coping style of cancer patients were positively correlated with death anxiety ( P<0.05) . Medical coping styles played a partial mediating role between psychological resilience and death anxiety, and the mediating effect accounted for 32.47% of the total effect. Conclusions:Clinical medical staff should pay attention to the death anxiety of cancer patients, improve their psychological resilience and encourage patients to actively cope with the disease, so as to reduce their death anxiety level and promote mental health.

Clostridioides difficile is a key pathogen of antibiotic related diarrhea and hospital associated infection, causing several outbreaks in Europe and North Americans and resulting in severe disease burden. However, the standardized diagnostic principle and detection specifications in C. difficile infection (CDI) survey are limited in China, and the infection rate and disease burden of CDI in China are unclear. Therefore, National Institute for Communicable Disease Control and Prevention,National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, together with another 11 institutions, draft the group standard entitled "Diagnosis of Clostridium difficile infection (T/CPMA 008-2020)" of Chinese Preventive Medicine Association. Based on the principle of "legality, scientificity, advancement, and feasibility", this standard clarifies risk factors, diagnosis principles, diagnoses and differential diagnoses in order to improve the accuracy of CDI diagnosis in clinical practice, guide the surveillance for CDI, and understand the infection rate and disease burden of CDI in China .

Antibiotic resistance (AR) is a severe and fast-growing public health challenge with rapid globalization, especially in China. Although some monitoring systems were established in different fields, fragmentation of information failed to show the overall trend and spread of AR. It is necessary to establish a national monitoring system to reveal the occurrence, development, and spread of AR. The new AR monitoring system needs an updated analysis indicators system. We intend to recommend a new analysis indicators system for AR was constructed and applied to AR data monitoring and analysis for humans, animals, the environment, and foods. After investigating and analyzing the 5 Chinese major AR monitoring systems and literature, we have formulated 15 AR monitoring analysis indicators and initially established an evaluation system for the country's new AR monitoring system.

Objective: To establish a standard operation procedure (SOP) for ribosome genotyping (ribotyping) on Clostridioides (C.) difficile, supplement and verify ribotyping typing library, so as to improve the comparability of data between different laboratories and to develop surveillance network of C. difficil in China. Methods: Molecular typing of 54 reference strains from the United States and Europe of C. difficile were performed by using the SOP referencing correspondence from abroad and from our laboratory with a BioNumerics 7.6 software to estimate the reference library of types of C. difficile. Identification of 374 clinical and animal isolates of C. difficile from 13 cities in China between 2010 and 2018, to supplement the library information. Kappa test was used to evaluate the consistency. Results: Results of capillary electrophoresis of reference strains appeared clear and stable, which guaranteed the clustering results being fast and accurate. Results from the supplementary typing showed that there were 84 types of isolates, of which 25 RT types were consistent with reference strains from abroad, while 58 RT types were different from referenced types. In the 40 referenced types, 15 RT types were not found in this study. In the consistency evaluation, the Kappa value was 0.891 and (P<0.01), showing the two Molecular typing as consistent and with close resemblance. Conclusions The result of capillary electrophoresis by applying SOP for ribotyping on C. difficile base on QIAxcel capillary electrophoresis system, appeared clear and stable. The standardized library seemed more easily used for comparability and data sharing between the laboratories.

Recent years, national laws and government policies were published as series to encourage the development of group standardizations. The updated Standardization Law of the People's Republic of China, implemented on January 1^st, 2018, stipulates that group standard is a part of the Chinese standard system. Under the current supportive circumstances, more institutes and organizations have joined in the writing and releasing procedures of group standards’. Despite the rapid development of group standardization to publish, we are still at the phase of exploring and regulating group standardizations. This review summarizes the development and practice on the development group standardization in the Chinese Preventive Medicine Association and analyzes current condition and deficiency of the work in China, in order to develop suggestions and strategies to improve and regulate group standardization.