Objective:To express and block the VP7 protein of human group H rotavirus (group H rotavirus, RVH) J19, and to prepare the polyclonal adsorbent polyantibody function of VP7 for identification.Methods:The VP7 protein of human H rotavirus J19 strain was expressed by baculovirus system, and the protein was blocked by affinity chromatography and hydrophobic chromatography. The VP7 polyantiserum was prepared by immunizing the VP7 protein of human H group H rotavirus J19, and the VP7 polyantiserum was identified by ELISA, Western blot (WB) and immunofluorescence.Results:Soluble J19 VP7 protein was obtained. J19 VP7 rabbit polyclonal antibody was prepared. ELISA showed that VP7 polyclonal antibody had a good binding effect, binding to human RVH VP7 protein at 2 048 000-fold. WB showed that the polyclonal antibody could bind to J19 VP7 protein; Immunofluorescence assay showed that VP7 polyclonal antibody could specifically recognize human group H rotavirus J19.Conclusions:The J19 VP7 protein of human H rotavirus was successfully obtained, and the prepared VP7 polyclonal antibody could recognize human H rotavirus, which provided a basis for the detection of human H rotavirus and the study of the structure and function of the viral capsid.

Objective:To express and block the VP7 protein of human group H rotavirus (group H rotavirus, RVH) J19, and to prepare the polyclonal adsorbent polyantibody function of VP7 for identification.Methods:The VP7 protein of human H rotavirus J19 strain was expressed by baculovirus system, and the protein was blocked by affinity chromatography and hydrophobic chromatography. The VP7 polyantiserum was prepared by immunizing the VP7 protein of human H group H rotavirus J19, and the VP7 polyantiserum was identified by ELISA, Western blot (WB) and immunofluorescence.Results:Soluble J19 VP7 protein was obtained. J19 VP7 rabbit polyclonal antibody was prepared. ELISA showed that VP7 polyclonal antibody had a good binding effect, binding to human RVH VP7 protein at 2 048 000-fold. WB showed that the polyclonal antibody could bind to J19 VP7 protein; Immunofluorescence assay showed that VP7 polyclonal antibody could specifically recognize human group H rotavirus J19.Conclusions:The J19 VP7 protein of human H rotavirus was successfully obtained, and the prepared VP7 polyclonal antibody could recognize human H rotavirus, which provided a basis for the detection of human H rotavirus and the study of the structure and function of the viral capsid.

Due to the deep anatomical location which is adjacent to the important organization, the operation on the diseases of lateral skull base area is still a challenging problem for neurosurgeons. This article briefly describes the history of the lateral skull base surgical approaches, advantages and disadvantages of each approach, indication and relative contraindication, and enumerates the application of neuroendoscope in the corresponding area, so as to offer help to choose the appropriate surgical approach according to the clinical characteristics; the application prospect of neuroendoscope in the relevant regions is discussed.