To address the insufficiency of domestic small-dose Chinese herbal granule dispensing equipment,this study introduces the"black box method"design concept and,on this basis,designs a lightweight,compact and highly accurate dispensing device.This device uses a two-phase four-wire brushless motor to drive the slider for displacement,thereby controlling the volume of the medicine falling into the weighing device to complete the weighing of the medicine.Finally,a two-phase hybrid stepping motor is used to drive the weighing device to transfer the medicine.This paper first elaborates in detail on the mechanical structure and weighing dispensing principle of this device.Secondly,the ANSYS finite element software is used to conduct a force analysis test on the weighing device,and it is concluded that the deformation of the weighing device during the working process is 9.98×10-5 mm,and the maximum deformation of the discharge port is 6.65×10-5 mm.Finally,the ADAMS software is used to simulate and analyze the motion process of the mechanism.The results show that,under the premise of ensuring the accuracy of weighing,there are no virtual points or interference in the motion process.On this basis,a prototype test is conducted,and the error accuracy of the dispensing machine is about 0.066 g,which is higher than the traditional dispensing accuracy.This equipment has strong adaptability and high dispensing accuracy,providing a new solution for small-dose precise quantitative equipment.

To address the insufficiency of domestic small-dose Chinese herbal granule dispensing equipment,this study introduces the"black box method"design concept and,on this basis,designs a lightweight,compact and highly accurate dispensing device.This device uses a two-phase four-wire brushless motor to drive the slider for displacement,thereby controlling the volume of the medicine falling into the weighing device to complete the weighing of the medicine.Finally,a two-phase hybrid stepping motor is used to drive the weighing device to transfer the medicine.This paper first elaborates in detail on the mechanical structure and weighing dispensing principle of this device.Secondly,the ANSYS finite element software is used to conduct a force analysis test on the weighing device,and it is concluded that the deformation of the weighing device during the working process is 9.98×10-5 mm,and the maximum deformation of the discharge port is 6.65×10-5 mm.Finally,the ADAMS software is used to simulate and analyze the motion process of the mechanism.The results show that,under the premise of ensuring the accuracy of weighing,there are no virtual points or interference in the motion process.On this basis,a prototype test is conducted,and the error accuracy of the dispensing machine is about 0.066 g,which is higher than the traditional dispensing accuracy.This equipment has strong adaptability and high dispensing accuracy,providing a new solution for small-dose precise quantitative equipment.

Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a rare autosomal recessive hereditary mitochondrial disease, which is mainly characterized by gastrointestinal and neurological system involvement. This article reports a rare case of MNGIE which presented as chronic diarrhea. The purpose is to improve the understanding of MNGIE.

Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a rare autosomal recessive hereditary mitochondrial disease, which is mainly characterized by gastrointestinal and neurological system involvement. This article reports a rare case of MNGIE which presented as chronic diarrhea. The purpose is to improve the understanding of MNGIE.

OBJECTIVETo establish red-green dual-color fluorescence glioma model in nude mice and to explore its practical values.

METHODSCM-DiI-stained rat glioma C6 cells (C6-CM- DiI cells) expressing red fluorescence were inoculated into the brain of athymic nude mice expressing green fluorescence protein (NC-C57BL/6J-EGFP). Then the whole-body dual-color fluorescence imaging was detected dynamically. Finally whole brains of the tumor-bearing mice were removed and 5 µm thick serial frozen slices were made. Light microscopy, fluorescence microscopy and confocal laser scanning microscopy were performed to observe the transplanted tumor tissue structure and fluorescent cells.

RESULTSTumor mass with red fluorescence increased gradually under continuous in-vivo fluorescence imaging monitoring. Under the fluorescence microscope, cells with red, green and yellow fluorescence were observed in the frozen sections of transplanted tumor tissue and the mutual structural relationship among them could be defined. The tumor cells migration, implantation and cell fusion between transplanted tumor cells and host cells could be observed. It could be distinguished according to the fluorescence, that blood vessels of tumor-origin displayed red fluorescence, blood vessels of host-origin displayed green fluorescence and mosaic blood vessels appeared yellow fluorescence. It was depicted that host innate astrocytes and oligodendrocytes in the microenvironment at the tumor periphery could be activated and dedifferentiated into nestin-positive cells.

CONCLUSIONSIn contrast to traditional animal model, the dual-color fluorescence imaging of nude mouse models of glioma possesses enormous advantages in investigating tumor mass in-vivo fluorescence imaging, tumor cells migration and metastasis, tumor angiogenesis and reactive activation of host innate cells in the microenvironment at tumor periphery, thus, has highly practical application value.

Animals ; Astrocytes ; metabolism ; Brain Neoplasms ; blood supply ; metabolism ; pathology ; ultrastructure ; Carbocyanines ; metabolism ; Cell Fusion ; Cell Line, Tumor ; Cell Movement ; Disease Models, Animal ; Fluorescent Dyes ; metabolism ; Glioma ; blood supply ; metabolism ; pathology ; ultrastructure ; Green Fluorescent Proteins ; metabolism ; Luminescent Proteins ; metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Nude ; Microscopy, Confocal ; Microscopy, Fluorescence ; Neoplasm Transplantation ; Neovascularization, Pathologic ; Nestin ; metabolism ; Oligodendroglia ; metabolism ; Rats

Objective To investigate the value of MRI in thmmbolytic thempy of hyperacute cerebral arterial thrombosis.Methods One hundred and sixteen patients with acute cerebral arterial thrombosis were recruited.plain CT and multi.modal MRI were performed in all patients.Thirty-three patients with hyperacute cerebral infarction were treated bv recombinant tissue plasminogen activator(rt-PA) and followed-up periodically using MRI.Results The 33 patients with thrombolysis selected by MRI demonstrated clinical improvement.90 d moclified Rankin scale scores(mRs)were less than 2 and life quality Barthal indexes(BI)were from 80 to 100.The complication included one asymptomatic parenchymal hematoma(PH1)one weeks after thrombolytic therapy and 4(12.2%)hemorrhagic infarction(HI)6 to 24 hours after thrombolytic therapy.Condusions MRI has significant clinical value for the screening and follow-up of intravenous thrombolytic therapy of hyperacute ischemic stroke.MRI-based thrombolysis is a safe and effective method for hyperacute ischemic stroke.