Objective:To evaluate the feasibility of a novel flexible ultrasound imaging catheter for high-resolution real-time imaging of deep abdominal structures via percutaneous intervention.Methods:A self-developed flexible ultrasound catheter system（outer diameter：3.3 mm，integrated with a 10.5 MHz high-frequency linear array transducer and 4-degree-of-freedom active deflection）was used to image abdominal organs（liver，gallbladder，pancreas，etc.）in two healthy beagles via percutaneous puncture，with comparison to conventional surface convex array probes（3.6 MHz）. Image quality was assessed using a double-blind design，independently scored by five ultrasound physicians with over 10 years of experience on a 6-point scale. Mixed-effects ordinal logistic regression（adjusted for dog，organ，and evaluator variability）was employed for inter-group comparisons，with effect sizes quantified by Cliff's delta. Characteristic anatomical structure detection rates were analyzed using generalized linear mixed models（Firth correction for zero events）. Inter-rater reliability was assessed via intraclass correlation coefficient（ICC，two-way random model）and Kendall's coefficient of concordance.Results:The flexible catheter group demonstrated significantly superior image quality compared to the surface probe group . The most pronounced improvement was observed in adrenal imaging（5 points vs. 1 point， P<0.001，δ=0.92），while pancreatic imaging showed no significant difference（2 points vs. 2 points， P=0.812，δ=0.05）. Other organs exhibited significant quality enhancements（all P<0.05，δ=0.63～0.81）. The catheter significantly improved detection rates of characteristic structures：gastric wall stratification（90% vs. 10%， OR=36.0， P<0.001），adrenal corticomedullary differentiation（80% vs. 0， OR=∞， P<0.001），and gallbladder/small intestinal wall trilaminar structure（80% vs. 20%， OR=12.0， P=0.003）. The high-quality image rate（≥5 points）reached 77.8%，representing an 11.6-fold improvement over surface ultrasound（6.7%， P<0.001）. Inter-rater reliability was excellent（ICC=0.85，W=0.79）. Conclusions:Percutaneous flexible ultrasound catheter imaging significantly enhances resolution and anatomical detail visualization of deep abdominal structures. Combining minimally invasive access with operational flexibility，this technology shows promise for providing precise image guidance in minimally invasive procedures.

Objective:To evaluate the long-term protective efficacy of the recombinant Chinese hamster ovary cell-derived hepatitis B vaccine（CHO-HepB）26 years post-vaccination in the rural China.Methods:Zhengding county，Hebei province was designated as a rural monitoring site for CHO-HepB efficacy. Study participants included individuals born between 1997 and 1999 who had completed the three-dose CHO-HepB primary series without booster doses. A cross-sectional survey was conducted in late 2024 using random sampling. Demographic and vaccination history data were collected via questionnaires，and hepatitis B virus（HBV）serological markers were detected using chemiluminescence. Historical surveillance data were integrated to infer infection statuses of HBsAg-positive individuals and evaluate longitudinal trends in anti-HBs seropositivity and antibody titers.Results:Among 178 participants（mean time since vaccination：26.2 years），the seroprevalence rates were 0.6% for HBsAg（95% CI：0.0%-1.6%），64.6% for anti-HBs（95% CI：57.6%-71.6%），and 1.1% for anti-HBc（95% CI：0.0%-2.7%）. Compared to the pre-vaccination baseline HBsAg positivity of 11.3% in children under 10 years of age，the estimated vaccine protection rate was 95%. Two notable cases were identified：one with concurrent HBsAg and anti-HBc positivity and one with anti-HBs and anti-HBc positivity，suggestive of transient HBV exposure（1999—2009）without chronicity. Natural immune boosting was inferred for the latter case based on anti-HBs titer dynamics. Longitudinal analysis of four prior cross-sectional surveys（2005，2009，2013，and 2017）revealed no significant upward trends in HBsAg and anti-HBc positivity（both P>0.05）over 26 years，while anti-HBs seropositivity declined significantly（ P<0.05）from 6 to 26 years post-vaccination. Conclusion:The CHO-HepB vaccine demonstrates sustained immunological persistence and robust long-term protection up to 26 years post-immunization. Continued emphasis on rigorous implementation of mother-to-child transmission prevention strategies is critical for future hepatitis B control.

Primary ciliary dyskinesia (PCD) is a clinically rare, genetically and phenotypically heterogeneous condition characterized by chronic respiratory tract infections, male infertility, tympanitis, and laterality abnormalities. PCD is typically resulted from variants in genes encoding assembly or structural proteins that are indispensable for the movement of motile cilia. Here, we identified a novel nonsense mutation, c.466G>T, in cilia- and flagella-associated protein 300 ( CFAP300 ) resulting in a stop codon (p.Glu156*) through whole-exome sequencing (WES). The proband had a PCD phenotype with laterality defects and immotile sperm flagella displaying a combined loss of the inner dynein arm (IDA) and outer dynein arm (ODA). Bioinformatic programs predicted that the mutation is deleterious. Successful pregnancy was achieved through intracytoplasmic sperm injection (ICSI). Our results expand the spectrum of CFAP300 variants in PCD and provide reproductive guidance for infertile couples suffering from PCD caused by them.
Adult ; Female ; Humans ; Male ; Pregnancy ; China ; Ciliary Motility Disorders/genetics* ; Codon, Nonsense ; East Asian People/genetics* ; Exome Sequencing ; Homozygote ; Infertility, Male/genetics* ; Kartagener Syndrome/genetics* ; Pedigree ; Sperm Injections, Intracytoplasmic ; Cytoskeletal Proteins/genetics*

Objective To construct a graded nursing program for exercise rehabilitation of children with hematopoietic stem cell transplantation(HSCT)and carry out a preliminary application,aiming to provide a basis for healthcare personnel to guide the exercise rehabilitation of children with HSCT.Methods Literature analysis and Delphi expert correspondence were used to construct a graded nursing program of exercise rehabilitation for children with HSCT.Through the convenience sampling method,children with HSCT in the hematology department of a tertiary-level children's hospital in Suzhou City were selected as the study subjects from June-December 2023,of which 26 children with HSCT from September-December 2023 served as an experimental group,and 23 children with HSCT from June-August 2023 as a control group.The experimental group was applied with the graded nursing program of exercise rehabilitation,and the control group was applied with the conventional nursing care.The comparison of cardiorespiratory fitness and adverse events between the 2 groups after 8 weeks of exercise intervention was conducted,and the adherence to the exercise intervention programme in the experimental group was recorded.Results The final constructed graded care program of exercise rehabilitation for children with HSCT included 6 primary indicators,20 secondary indicators,and 44 tertiary indicators.Finally,21 children in the experimental group and 23 children in the control group were included in the study.After the intervention,the 6 min walking distance of the experimental group increased compared with that of the control group,and the difference was statistically significant(P＜0.001).85.71％of children in the experimental group had good exercise compliance,and there was no adverse events in both groups.Conclusion The constructed graded nursing program of exercise rehabilitation for children with HSCT is scientific,reasonable,safe and feasible,which can improve the cardiorespiratory capacity of children with HSCT and provide the basis for their exercise rehabilitation.

Objective:To study the clinical and pathological features, immunophenotypes, molecular genetics characteristics, differential diagnosis, and prognostic factors of pulmonary and tracheal glomus tumors.Method:Eight cases of pulmonary and tracheal glomus tumors were collected in Jiangsu Provincial Hospital (including 1 consultation, from Gaochun People's Hospital, Nanjing, China) between May 2015 and April 2023, and their clinical and imaging data, pathological morphology, and immunohistochemical characteristics were retrospectively analyzed. Gene testing and follow-up were performed.Result:There were 5 males and 3 females with onset ages ranging from 29 to 75 years old, median age 63.5 years. Among the patients, 5 cases were located in the trachea and 3 cases in the lungs. Under light microscopy, 5 cases were benign glomus tumors with clear boundaries, diffuse sheet or nest-like distribution, small, round or short spindle-shaped tumor cells, rounded and centered nuclei, and no obvious nuclear mitosis was seen. Two cases of glomus tumor of uncertain malignant potential showed an infiltrative growth pattern involving smooth muscle, nerves and blood vessels with necrosis and calcification, the tumor cells were more uniform in size, round or short spindle-shaped nuclei, and no obvious nuclear mitosis was seen; One case of malignant glomus tumor was seen in sarcomatous areas, lung membrane invasion and necrosis, the tumor cells were highly heterogeneous, binucleated and multinucleated, with nuclear mitoses of 20/50 high power field (HPF), and pathologic nuclear mitoses were easy to be seen. Immunohistochemically, SMA, Calponin, H-caldesmon, Vimentin and Collagen Ⅳ were all positive (8/8). Some cases expressed Syn (3/8) and Bcl-2 (4/8). The Ki-67 proliferation index was 1-2% (7/8) and 40% (1/8). BRAF V600E were detected as wild-type (8/8), and no mutations were detected in exons 2, 3, and 4 of KRAS human EML4- ALK fusion gene were negative in 5 surgical cases. Case 6 showed HMBOX1- ALK gene fusion, TERT gene mutation and CDKN2A gene deletion, and case 8 showed CARMN- NOTCH2 gene fusion. Seven cases were followed up (8-103 months, median follow-up time 30 months), 1 case was lost, 1 case recurred 21 months after surgery, and others with no evidence of recurrence or metastasis. Conclusions:Pulmonary and tracheal glomus tumors are very rare and need to be differentiated from other common tumors by combining pathological morphology and immunohistochemistry. Maybe there are some differences in the malignant diagnostic criteria and molecular genetic characteristics between visceral derived glomus tumors and soft tissue derived tumors of the same kind, such as limbs and skin. More data accumulation is needed.

Objective:To explore the effects of quality control circles (QCCs) combined with the strength concept in health education for pediatric leukemia patients.Methods:Totally 185 pediatric leukemia patients admitted to the Children's Hospital of Soochow University from January 2021 to August 2023 were selected as participants by convenience sampling. They were randomly divided into a control group ( n=92) and an observation group ( n=93) based on odd and even numbering. Patients in the control group received standard health education, while those in the observation group underwent QCC-based health education activities incorporating the strength concept. Dietary behavior improvement, treatment adherence, quality of life, and patient satisfaction with nursing care were compared between the two groups before and after the intervention. Results:After the intervention, dietary behavior scores in both groups decreased significantly, with the observation group showing significantly lower scores than the control group ( P<0.05). Treatment adherence and quality of life scores improved significantly in both groups, with the observation group scoring higher than the control group across all dimensions ( P<0.05). Moreover, the observation group demonstrated higher patient satisfaction with nursing care compared to the control group ( P<0.05) . Conclusions:The application of QCC activities combined with health education based on the strength concept effectively improves dietary behavior, treatment adherence, quality of life, and satisfaction with nursing care among pediatric leukemia patients, which is worth promoting in clinical practice.

Objective To construct a graded nursing program for exercise rehabilitation of children with hematopoietic stem cell transplantation(HSCT)and carry out a preliminary application,aiming to provide a basis for healthcare personnel to guide the exercise rehabilitation of children with HSCT.Methods Literature analysis and Delphi expert correspondence were used to construct a graded nursing program of exercise rehabilitation for children with HSCT.Through the convenience sampling method,children with HSCT in the hematology department of a tertiary-level children's hospital in Suzhou City were selected as the study subjects from June-December 2023,of which 26 children with HSCT from September-December 2023 served as an experimental group,and 23 children with HSCT from June-August 2023 as a control group.The experimental group was applied with the graded nursing program of exercise rehabilitation,and the control group was applied with the conventional nursing care.The comparison of cardiorespiratory fitness and adverse events between the 2 groups after 8 weeks of exercise intervention was conducted,and the adherence to the exercise intervention programme in the experimental group was recorded.Results The final constructed graded care program of exercise rehabilitation for children with HSCT included 6 primary indicators,20 secondary indicators,and 44 tertiary indicators.Finally,21 children in the experimental group and 23 children in the control group were included in the study.After the intervention,the 6 min walking distance of the experimental group increased compared with that of the control group,and the difference was statistically significant(P＜0.001).85.71％of children in the experimental group had good exercise compliance,and there was no adverse events in both groups.Conclusion The constructed graded nursing program of exercise rehabilitation for children with HSCT is scientific,reasonable,safe and feasible,which can improve the cardiorespiratory capacity of children with HSCT and provide the basis for their exercise rehabilitation.

Objective:To explore the effects of quality control circles (QCCs) combined with the strength concept in health education for pediatric leukemia patients.Methods:Totally 185 pediatric leukemia patients admitted to the Children's Hospital of Soochow University from January 2021 to August 2023 were selected as participants by convenience sampling. They were randomly divided into a control group ( n=92) and an observation group ( n=93) based on odd and even numbering. Patients in the control group received standard health education, while those in the observation group underwent QCC-based health education activities incorporating the strength concept. Dietary behavior improvement, treatment adherence, quality of life, and patient satisfaction with nursing care were compared between the two groups before and after the intervention. Results:After the intervention, dietary behavior scores in both groups decreased significantly, with the observation group showing significantly lower scores than the control group ( P<0.05). Treatment adherence and quality of life scores improved significantly in both groups, with the observation group scoring higher than the control group across all dimensions ( P<0.05). Moreover, the observation group demonstrated higher patient satisfaction with nursing care compared to the control group ( P<0.05) . Conclusions:The application of QCC activities combined with health education based on the strength concept effectively improves dietary behavior, treatment adherence, quality of life, and satisfaction with nursing care among pediatric leukemia patients, which is worth promoting in clinical practice.

Objective:To evaluate the long-term protective efficacy of the recombinant Chinese hamster ovary cell-derived hepatitis B vaccine（CHO-HepB）26 years post-vaccination in the rural China.Methods:Zhengding county，Hebei province was designated as a rural monitoring site for CHO-HepB efficacy. Study participants included individuals born between 1997 and 1999 who had completed the three-dose CHO-HepB primary series without booster doses. A cross-sectional survey was conducted in late 2024 using random sampling. Demographic and vaccination history data were collected via questionnaires，and hepatitis B virus（HBV）serological markers were detected using chemiluminescence. Historical surveillance data were integrated to infer infection statuses of HBsAg-positive individuals and evaluate longitudinal trends in anti-HBs seropositivity and antibody titers.Results:Among 178 participants（mean time since vaccination：26.2 years），the seroprevalence rates were 0.6% for HBsAg（95% CI：0.0%-1.6%），64.6% for anti-HBs（95% CI：57.6%-71.6%），and 1.1% for anti-HBc（95% CI：0.0%-2.7%）. Compared to the pre-vaccination baseline HBsAg positivity of 11.3% in children under 10 years of age，the estimated vaccine protection rate was 95%. Two notable cases were identified：one with concurrent HBsAg and anti-HBc positivity and one with anti-HBs and anti-HBc positivity，suggestive of transient HBV exposure（1999—2009）without chronicity. Natural immune boosting was inferred for the latter case based on anti-HBs titer dynamics. Longitudinal analysis of four prior cross-sectional surveys（2005，2009，2013，and 2017）revealed no significant upward trends in HBsAg and anti-HBc positivity（both P>0.05）over 26 years，while anti-HBs seropositivity declined significantly（ P<0.05）from 6 to 26 years post-vaccination. Conclusion:The CHO-HepB vaccine demonstrates sustained immunological persistence and robust long-term protection up to 26 years post-immunization. Continued emphasis on rigorous implementation of mother-to-child transmission prevention strategies is critical for future hepatitis B control.

Objective:To evaluate the feasibility of a novel flexible ultrasound imaging catheter for high-resolution real-time imaging of deep abdominal structures via percutaneous intervention.Methods:A self-developed flexible ultrasound catheter system（outer diameter：3.3 mm，integrated with a 10.5 MHz high-frequency linear array transducer and 4-degree-of-freedom active deflection）was used to image abdominal organs（liver，gallbladder，pancreas，etc.）in two healthy beagles via percutaneous puncture，with comparison to conventional surface convex array probes（3.6 MHz）. Image quality was assessed using a double-blind design，independently scored by five ultrasound physicians with over 10 years of experience on a 6-point scale. Mixed-effects ordinal logistic regression（adjusted for dog，organ，and evaluator variability）was employed for inter-group comparisons，with effect sizes quantified by Cliff's delta. Characteristic anatomical structure detection rates were analyzed using generalized linear mixed models（Firth correction for zero events）. Inter-rater reliability was assessed via intraclass correlation coefficient（ICC，two-way random model）and Kendall's coefficient of concordance.Results:The flexible catheter group demonstrated significantly superior image quality compared to the surface probe group . The most pronounced improvement was observed in adrenal imaging（5 points vs. 1 point， P<0.001，δ=0.92），while pancreatic imaging showed no significant difference（2 points vs. 2 points， P=0.812，δ=0.05）. Other organs exhibited significant quality enhancements（all P<0.05，δ=0.63～0.81）. The catheter significantly improved detection rates of characteristic structures：gastric wall stratification（90% vs. 10%， OR=36.0， P<0.001），adrenal corticomedullary differentiation（80% vs. 0， OR=∞， P<0.001），and gallbladder/small intestinal wall trilaminar structure（80% vs. 20%， OR=12.0， P=0.003）. The high-quality image rate（≥5 points）reached 77.8%，representing an 11.6-fold improvement over surface ultrasound（6.7%， P<0.001）. Inter-rater reliability was excellent（ICC=0.85，W=0.79）. Conclusions:Percutaneous flexible ultrasound catheter imaging significantly enhances resolution and anatomical detail visualization of deep abdominal structures. Combining minimally invasive access with operational flexibility，this technology shows promise for providing precise image guidance in minimally invasive procedures.