Objective: To explore the completion status and related factors of preventive anti tuberculosis therapy among close contacts of pulmonary tuberculosis cases in schools across Guizhou Province, so as to provide scientific evidence for optimizing preventive treatment strategies and raising the treatment completion rate among student close contacts. Methods: Demographic, epidemiological and preventive treatment related data were extracted from the Guizhou Disease Prevention and Control Cloud Platform-School Tuberculosis Management System. Totally 3 044 student subjects with latent tuberculosis infection (LTBI) who received the 3HR regimen and had final treatment outcomes between January 1, 2023 and December 31, 2025 were enrolled in the study. The χ 2 test, Mann Whitney U- test and binary Logistic regression model were adopted to analyze factors associated with the completion of preventive treatment among the student LTBI population. Results: The overall completion rate of preventive treatment was 74.24%. The prevention treatment completion rate declined with prolonged medication duration( χ 2 trend =252.09, P <0.01). Binary Logistic regression analysis indicated that students from private schools had higher odds of incomplete treatment compared with those from public schools ( OR =1.32, P <0.05). Compared with college students, students in primary school and below, junior high school, senior high school, secondary vocational school exhibited lower odds of incomplete treatment ( OR =0.35, 0.31, 0.59, 0.17,all P <0.05). Compared with contacts exposed in dormitories, classroom exposed and same floor or others exposed contacts showed higher odds of incomplete treatment ( OR =4.28, 8.59, both P <0.05). Participants with an interval exceeding 2 weeks from screening to treatment initiation were more likely to complete preventive treatment ( OR =0.80, P <0.05). Conclusions The overall completion rate of preventive treatment remains unsatisfactory among students with LTBI in Guizhou Province. Multiple interventions are recommended to improve treatment adherence and completion rates in the population, including implementing diversified medication supervision measures, strengthening public health management and supervision for private schools, establishing efficient referral and management mechanisms to enhance students treatment completion.

Objective:To investigate the protective effect of berberine hydrochloride on intestinal mucosal barrier damage in sepsis rats and its mechanism.Methods:Forty-eight male SD rats were divided into a control group (Sham group, 6 cases), a sepsis model group (LPS group, 14 cases), a berberine hydrochloride intervention group (Ber group, 14 cases), and a Notch signaling pathway inhibition group (DAPT group, 14 cases) according to random number table method. The DAPT group was intraperitoneally injected with 5 mg/kg Notch signaling pathway inhibition DAPT 2 hours before modeling. The sepsis model was established by intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS); Sham group was injected with an equal amount of saline (2 mL). The Ber group and DAPT group were treated with gavage of 50 mg/kg berberine hydrochloride 2 hours after modeling; Sham group and LPS group were treated with gavage of an equal amount of saline (2 mL). The temperature, weight, behavior and survival rate of rats were observed at 0, 6, 12 and 24 hours of modeling. After 24 hours of modeling, abdominal aortic blood was collected under anesthesia, and intestinal tissues were obtained after euthanasia. The pathological changes of ileum were observed under light microscope. The ultrastructure of ileum was observed under transmission electron microscope. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum diamine oxidase (DAO), intestinal fatty acid binding protein (iFABP), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Real time-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the mRNA and protein expressions of tight junction proteins (Occludin and Claudin1), Notch1 and their downstream target signals in the ileum tissue.Results:After 24 hours of modeling, compared with the Sham group, the LPS group, Ber group, and DAPT group showed a decrease in weight and an increase in temperature. Among them, the LPS group showed the most significant changes, followed by the DAPT group, and the Ber group showed the least significant changes. The survival rates of the LPS group, Ber group, and DAPT group were all lower than those of the Sham group [42.9% (6/14), 57.1% (8/14), 57.1% (8/14) vs. 100% (6/6)], and six rats were taken from each group for subsequent testing. Macroscopic observation of the intestine showed that the LPS group had the most severe edema in the ileum tissue and abdominal bleeding, with significant improvement in the Ber group and followed by the DAPT group. Under the light microscope, the LPS group showed disordered arrangement of glandular tissue in the ileum mucosa, significantly reduced goblet cells, and extensive infiltration of inflammatory cells, which were significantly improved in the Ber group but less improved in the DAPT group. Under electron microscopy, the LPS group showed extensive shedding of ileal microvilli and severe damage to the tight junction complex structure of intestinal epithelial cells, which was significantly improved in the Ber group but less improved in the DAPT group. The levels of serum DAO, iFABP, TNF-α, IL-6 in the LPS group were significantly higher than those in the Sham group, while the above indicators in the Ber group were significantly lower than those in the LPS group [DAO (μg/L): 4.94±0.44 vs. 6.53±0.49, iFABP (ng/L): 709.67±176.97 vs. 1 417.71±431.44, TNF-α (ng/L): 74.70±8.15 vs. 110.36±3.51, IL-6 (ng/L): 77.34±9.80 vs. 101.65±6.92, all P < 0.01], while the above indicators in the DAPT group were significantly higher than those in the Ber group. The results of RT-PCR and Western blotting showed that the mRNA and protein expressions of Occludin, Claudin1, Notch1, and Hes1 in the ileum tissue of LPS group rats were decreased compared to the Sham group, which were significantly increased in the Ber group compared with the LPS group [mRNA expression: Occludin mRNA (2 -ΔΔCt): 1.61±0.74 vs. 0.30±0.12, Claudin1 mRNA (2 -ΔΔCt): 1.97±0.37 vs. 0.58±0.14, Notch1 mRNA (2 -ΔΔCt): 1.29±0.29 vs. 0.36±0.10, Hes1 mRNA (2 -ΔΔCt): 1.22±0.39 vs. 0.27±0.04; protein expression: Occludin/GAPDH: 1.17±0.14 vs. 0.74±0.04, Claudin1/GAPDH: 1.14±0.06 vs. 0.58±0.10, Notch1/GAPDH: 0.87±0.11 vs. 0.56±0.09, Hes1/GAPDH: 1.02±0.13 vs. 0.62±0.01; all P < 0.05], while those in the DAPT group were significantly lower than those in the Ber group. Conclusion:Early use of berberine hydrochloride can significantly improve intestinal mucosal barrier damage in sepsis rats, and its mechanism may be related to inhibiting inflammatory response and regulating the expression of intestinal mechanical barrier tight junction protein through Notch1 signal.

Background Work-related musculoskeletal disorders (WMSDs) are one of the major occupational health problems in the world. Pharmaceutical industry is an important part of China's national economy. At present, there are few related studies reported at home and abroad. Objective To investigate the status and influencing factors of WMSDs in chemical pharmaceutical industry. Methods A cross-sectional epidemiological survey was conducted among all workers from three chemical pharmaceutical enterprises in Guangzhou. The Musculoskeletal Disorders Questionnaire was used to collect information on demographic characteristics, symptoms of musculoskeletal disorders, types of work, work organization, and and work postures. Multiple logistic regression method was used to analyze the risk factors of WMSDs in chemical pharmaceutical workers. Results In this study, 563 workers were selected as subjects. The total prevalence rate of WMSDs symptoms in the chemical pharmaceutical workers was 43.9% (247/563), and the leading body part-specific prevalence rate from high to low was 34.3% in the lower back, 24.3% in the upper back, 24.0% in the shoulders, and 23.8% in the neck. The prevalence rate of WMSDs symptoms in multiple body parts (30.0%) was 2.16 times higher than that in single body part (13.9%), and the prevalence rate of WMSDs symptoms in four body parts was the highest (11.4%). The results of multiple logistic regression analysis showed that age ≥50 years (reference age <30 years) (OR=2.140, 95%CI: 1.054-4.345), often or very often (reference never/rarely) long-time head rotating (OR=2.695, 95%CI: 1.753-4.142) and long-time keeping arms above shoulders (OR=1.902, 95%CI: 1.108-3.265) increased the risk of reporting WMSDs symptoms (P<0.05). Regarding education level, workers with high school and technical secondary school (OR=0.333, 95%CI: 0.175-0.636) or college and above (OR=0.413, 95%CI: 0.216-0.790) education had a lower risk of reporting WMSDs symptoms than those with middle school or below (P<0.05). Conclusion The prevalence rate of reporting WMSDs symptoms in chemical pharmaceutical industry is high, the involving body parts are lower back, upper back, shoulders, and neck, and reporting simultaneous occurrence of WMSDs symptoms in multiple body parts is common. The chemical pharmaceutical manufacturers can reduce the risk of WMSDs by strengthening the training on workers' ergonomics knowledge, paying attention to the less educated personnel, protecting the elderly workers, and avoiding awkward work postures, like rotating head for a long time and raising arms over shoulders.

{L-End}Objective To analyze the prevalence of multi-site work-related musculoskeletal disorders (WMSDs) and their influencing factors among workers in the chemical pharmaceutical industry. {L-End}Methods A total of 563 workers from three chemical pharmaceutical factories in Guangzhou City were selected as the research subjects using the judgment sampling method. The prevalence of multi-site WMSDs in the past 12 months was investigated using the electronic questionnaire of Chinese Version of Musculoskeletal Disorders Questionnaire. {L-End}Results The prevalence of multi-site WMSDs in the research subjects was 30.0% (169/563). The results of multivariable logistic regression analysis showed that workers who smoked regularly/frequently and those who had their hands above shoulder level for long periods of time had a lower risk of multi-site WMSDs than those who never/occasionally smoked (all P≤0.05). Workers with a high school, secondary specialty, college degree, or above had a lower risk of multi-site WMSDs than those with a middle school education or below (all P<0.05). The longer the daily working hours, the higher the risk of multiple WMSDs (all P<0.05). Workers with regular/frequently work shifts had a lower risk of multi-site WMSDs than those with none/occasional work shifts (P<0.05). {L-End}Conclusion The prevalence of multi-site WMSDs among workers in the chemical pharmaceutical industry is relatively high. The influencing factors are individual factors, unreasonable work organization and poor ergonomics factors.

Objective To systematically evaluate the risk of hypertension and serious hypertension due to vascular endothelial growth factor receptor tyrosine kinase inhibitors(VEGFR-TKIs)in patients with non-small cell lung cancer(NSCLC). Methods The related databases were searched for the randomized controlled trials(RCTs)on the hypertension induced by VEGFR-TKI before August 2017. The patients with NSCLC who were treated with VEGFR-TKIs were enrolled into the test group,the patients who received placebo and/or the patients in the test group who used the other drugs except VEGFR-TKI were enrolled into the control group. The outcomes of all RCTs were the incidence of hypertension and serious hypertension. The quality of the enrolled literature was evaluated by Jadad scoring system. The Meta-analysis was conducted by STATA 13.1 software. The results were expressed as relative risk(RR)and 95% confidence interval(CI). Results A total of 18 RCTs involving 10 020 patient were enrolled,including 5 274 in the test group and 4 746 in the control group. The result of literature′s quality evaluation showed 13 of high grade and 5 of low grade. The results of the Meta-analysis showed that the incidence of a hypertension and serious hypertension in test group were significantly higher than those in the control group,respectively [15.8%(834/5 274)vs. 3.8%(179/4 746),RR=4.11,95%CI:3.50-4.81,P<0.001;3.49%(184/5 274)vs. 0.70%(33/4 746),RR=4.64,95%CI:3.22-6.68,P<0.001]. The results of the subgroup analysis showed that the incidences of hypertension and serious hypertension in the vandetanib group were significantly higher than those in the control group[15.1%(397/2 625)vs. 2.3%(49/2 136),RR=3.72,95%CI:2.80-4.95,P<0.001;3.4%(90/2 625)vs. 0.6%(13/2 163),RR=5.99,95%CI:4.45-8.07,P <0.001];the incidences of hypertension and serious hypertension in the sorafenib group were significantly higher than those in the control group[15.7%(201/1 278)vs. 5.4%(68/1 249),RR=2.90,95%CI:2.22-3.79,P<0.001;4.0%(52/1 278)vs. 1.0%(12/1 249), RR=4.09,95%CI:2.21-7.55,P<0.001];the incidences of hypertension and serious hypertension in the cediranib group were significantly higher than those in the control group[32.1%(89/277)vs. 8.7%(24/276),RR=3.70,95%CI:2.43-5.62,P<0.001;12.2%(34/277)vs. 1.8%(5/276),RR=6.77,95%CI:2.68-17.08,P<0.001];and the incidence of hypertension in the motesanib group was significantly higher than that in the control group[26.1%(139/533)vs. 6.5%(35/539),RR=4.02, 95%CI:2.83-5.70,P<0.001]. Conclusion The risk of hypertension and serious hypertension may increase in the NSCLC patients who received VEGFR-TKIs.

Objective To systematically evaluate the risk of hypertension and serious hypertension due to vascular endothelial growth factor receptor tyrosine kinase inhibitors(VEGFR-TKIs)in patients with non-small cell lung cancer(NSCLC). Methods The related databases were searched for the randomized controlled trials(RCTs)on the hypertension induced by VEGFR-TKI before August 2017. The patients with NSCLC who were treated with VEGFR-TKIs were enrolled into the test group,the patients who received placebo and/or the patients in the test group who used the other drugs except VEGFR-TKI were enrolled into the control group. The outcomes of all RCTs were the incidence of hypertension and serious hypertension. The quality of the enrolled literature was evaluated by Jadad scoring system. The Meta-analysis was conducted by STATA 13.1 software. The results were expressed as relative risk(RR)and 95% confidence interval(CI). Results A total of 18 RCTs involving 10 020 patient were enrolled,including 5 274 in the test group and 4 746 in the control group. The result of literature′s quality evaluation showed 13 of high grade and 5 of low grade. The results of the Meta-analysis showed that the incidence of a hypertension and serious hypertension in test group were significantly higher than those in the control group,respectively [15.8%(834/5 274)vs. 3.8%(179/4 746),RR=4.11,95%CI:3.50-4.81,P<0.001;3.49%(184/5 274)vs. 0.70%(33/4 746),RR=4.64,95%CI:3.22-6.68,P<0.001]. The results of the subgroup analysis showed that the incidences of hypertension and serious hypertension in the vandetanib group were significantly higher than those in the control group[15.1%(397/2 625)vs. 2.3%(49/2 136),RR=3.72,95%CI:2.80-4.95,P<0.001;3.4%(90/2 625)vs. 0.6%(13/2 163),RR=5.99,95%CI:4.45-8.07,P <0.001];the incidences of hypertension and serious hypertension in the sorafenib group were significantly higher than those in the control group[15.7%(201/1 278)vs. 5.4%(68/1 249),RR=2.90,95%CI:2.22-3.79,P<0.001;4.0%(52/1 278)vs. 1.0%(12/1 249), RR=4.09,95%CI:2.21-7.55,P<0.001];the incidences of hypertension and serious hypertension in the cediranib group were significantly higher than those in the control group[32.1%(89/277)vs. 8.7%(24/276),RR=3.70,95%CI:2.43-5.62,P<0.001;12.2%(34/277)vs. 1.8%(5/276),RR=6.77,95%CI:2.68-17.08,P<0.001];and the incidence of hypertension in the motesanib group was significantly higher than that in the control group[26.1%(139/533)vs. 6.5%(35/539),RR=4.02, 95%CI:2.83-5.70,P<0.001]. Conclusion The risk of hypertension and serious hypertension may increase in the NSCLC patients who received VEGFR-TKIs.

In recent years,the prevalence of diabetes is increasing year by year with the improvement of people's living standard.The traditional oral anti-hyperglycemic drugs as well as insulin injection therapy can not block the progress of the disease course.The deterioration of glycaemic control results in various acute and chronic complications which seriously affect patients' health and bring huge economic burden.The researchers have been seeking new therapeutic regimens to improve or even reverse diabetes process.This paper focuses on the following novel treatment options such as GLP-1 receptor agonist,DPP4 inhibitors, fixed-dose combination,SGLT2 inhibitors,amylin analogues,dopamine receptor agonist,bile acid sequestrant,bariatric surgery and pancreatic stem cell transplantation.

Objective To investigate the anti-inflammatory and analgesic effects of extracts from Garden Balsam seeds in order to provide theoretical basis for rational clinical application. Methods The mouse ear swelling induced by xylene, the body torsion induced by acetic acid or hot plate method, the rat foot swelling induced by egg white and granuloma model were set up to study anti-inflammatory and analgesic effects of extracts from Garden Balsam seeds. Results The water extracts at the middle and high doses reduced the body torsion counts of mice (P<0. 05), alleviated the rat toe swelling, but did not affect mice ear swelling. The extracts at the high dose prolonged pain threshold at 60 min (P<0. 01) and 90 min (P<0. 05); the alcohol ex-tracts at the middle and high doses significantly lowered body twisting counts (P<0. 01), attenuated the ear swelling of mice (P<0. 05), remarkably prolonged the pain threshold (P<0. 01)at 30 min, weakened the rat foot swelling, and elevated 60 min pain threshold at the high doses (P<0. 01), but the extracts exerted no effects on the rats granuloma. Conclusion The extracts from Garden Balsam seeds have anti-inflammatory and analgesic effects.

Objective To study the effect of extracts of Smilax china L. on inhibition the experimentally induced benign prostatic hyperplasia ( BPH) , and screen the effective fraction. Methods The BPH model was built on the castrated rats by subcutaneous injection of testosterone propionate. Male rats were randomly divided into eight groups ( n=6 ):sham operation, model control, petroleum ether fraction, acetic ether fraction, n-butyl alcohol fraction, water fraction, macroporous resin fraction ( FMR) , and total extracts group. The rats were treated with testosterone propionate by subcutaneous injection for consecutive 3 weeks. Meanwhile, rats were orally administrated with the six extract fractions of S. china L. After the last administration, serum was separated for the determination of prostatic acid phosphatase ( PACP ) , prostate was weighed and histopathological examination was carried out to evaluate the inhibitory effect of S. china L. against BPH. Results All of the six fractions from S. china L. could inhibit BPH, and the n-butanol fraction, water fraction and FMR showed better inhibitory effect, which significantly decreased the prostatic index by 52. 80%, 50. 93% and 67. 70%, respectively, remarkably reduced serum PACP, and notably improved the prostate gland morphology compared with the model group. Among the three fractions, FMR showed the strongest effect against BPH. Conclusion S. china L. ameliorates the experimentally prostatic hyperplasia, and FMR showes the best effect, which might be the bioactive components against BPH.

Objective:To elucidate the clinical effect of xingxiong sodium chloride injection on vertebralbasilar insufficiency.Method:98 patients with vertebralbasilar nsufficiency were randomly divided into two groups,(therapy group with 50 cases treated with xingxiong sodium chloride injection and the controlled group with 48 treated with danshen injec- tion.After 14 days,their clinical symptom change and haemodynamics parameters change were evaluated and detected by transcranial Doppler (TCD).Result:In the therapy group,45 cases (90%) were cured and in the controlled,30 cases (62.5%) were cured.The two groups presented a statistical significance (P