Objective:To compare and evaluate the quality of wild and different cultivation methods of Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai through analysis on UPLC characteristic atlas and multi-component content determination results. Methods:UPLC was used to establish the characteristic chromatogram and multi-component content determination method of Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai, and clustering analysis, orthogonal partial least squares - discriminant analysis method were used for chemical pattern recognition analysis. Results:The results showed that there were 10 common peaks in 18 batches of Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai. Five components were identified, erythrothioneine(peak 1), protocatechuic acid (peak 2), protocatechualdehyde (peak 3), caffeic acid (peak 4) and Hispidin (peak 5). HCA and OPLS-DA could distinguish Sanghuang porus vaninii (Ljub.) with different cultivation methods. Conclusion:Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai in wood is closer to wild Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai than in substitute cultivation. The UPLC characteristic atlas and multi-component content determination method established in this study can provide reference for the quality evaluation of Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai.

Objective To explore the molecular mechanism of luteolin in the treatment of cervical cancer based on network pharmacology and molecular docking technology. Methods The drug-like properties of luteolin were analyzed by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The targets of luteolin were obtained from PharmMapper, Super-PRED, and Swiss Target Prediction databases. The targets related to cervical cancer were acquired from GeneCards, OMIM, and PharmGKB databases. The intersection targets of luteolin and cervical cancer were obtained through EVenn, and the "luteolin-intersection targets-cervical cancer" network diagram was constructed by Cytoscape 3.8.1. The STRING database was used to analyze the protein-protein interaction (PPI) network of intersection targets and screen the core targets. The Database for Annotation, Visualization and Integrated Discovery (David) was used to conduct Gene Ontology (GO) gene function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis of the targets. PyMoL 2.6.0, AutoDockTool 1.5.7 and OpenBabel 2.4.1 software were used to perform molecular docking between the core targets and luteolin. The survival analysis and pan-cancer analysis of the core targets were performed in the GEPIA database. Results A total of 449 targets of luteolin and 1 334 targets related to cervical cancer were obtained; there were 100 intersection targets between luteolin and cervical cancer, of which 24 were core targets, including MMP2, HRAS, MAPK1, AKT1, RHOA and PGR. GO and KEGG enrichment analyses revealed that the intersection targets participated in 455 biological processes, 70 cellular components, 119 molecular functions, and 143 KEGG signaling pathways. Molecular docking revealed a good binding of MMP2 with luteolin. The survival curves of cervical cancer patients showed that the risk ratios of RHOA, MAPK1, MMP2 and AKT1 genes were greater than 1, while those of HRAS and PGR were less than 1. Pan-cancer analysis showed that HRAS and MAPK1 were highly expressed in cervical cancer, and HRAS had significant expression differences. Conclusion Luteolin treats cervical cancer through a multi-target and multi-pathway mechanism.

Objective To analyze the characteristics of types of hearing curves in patients with tinnitus and the differences in tinnitus severity and treatment outcomes among patients with different types of hearing curves,to further explore the relationship between tinnitus and hearing loss,and to provide clinical evidence for exploring the etiology and treatment plans for tinnitus.Methods The basic information and the tinnitus and hearing data of the tinnitus patients who met the inclusion criteria were collected.The patients'severity of tinnitus was assessed on a scale of grades Ⅰ through Ⅴ according to the grading standards of the tinnitus evaluation questionnaire(TEQ).According to the classification criteria for the different types of hearing curves,the tinnitus patients were categorized into 6 types,including normal hearing,high-frequency descending type,low-frequency descending type,partial descending type,flat descending type,and total deafness.We analyzed in multiple aspects the tinnitus status identified at the initial diagnosis and the treatment outcomes at the last diagnoses and follow-up in patients with different types of hearing curves.Results A total of 672 patients with tinnitus were included in this study,of which 183 had normal hearing,and 489 had varying degrees of hearing loss.According to the TEQ grading criteria for tinnitus assessment,the patients'severity of tinnitus at the initial diagnosis was mainly concentrated in grades Ⅱ and Ⅲ,with 86 cases(12.79％)classified as grade Ⅰ,309 cases(45.98％)as grade Ⅱ,161 cases(23.96％)as grade Ⅲ,87 cases(12.95％)as grade Ⅳ,and 29 cases(4.32％)as grade Ⅴ.Regarding the distribution of hearing curve types,tinnitus patients had predominantly hearing curves of the high-frequency descending type.There were statistically significant differences in the distribution of tinnitus severity among patients with different types of hearing curves at the initial diagnosis(P＜0.01).Among tinnitus patients with high-frequency descending hearing curve,there was no statistically significant difference in the distribution of hearing loss severity among patients with tinnitus of different degrees of severity.After uniform treatment,there was no statistically significant difference in treatment outcomes between patients with different types of audiometry curves at the time of the last diagnosis and the follow-up.Conclusion Despite significant differences in the severity of tinnitus among patients with different types of hearing curves at the initial diagnosis in this study,no clear association was found between tinnitus treatment outcomes and hearing curve types.Furthermore,no clear association was found between tinnitus and the degree of hearing loss in patients with the same type of hearing curve.Hence,further clinical evidence is warranted to assess the efficacy of selecting tinnitus treatment plans that are similar to those for sudden deafness based on the types of hearing curves.

Objective To evaluate the short-term and long-term effect of comprehensive traditional Chinese medical therapy for regulating spleen and stomach in treating tinnitus. Methods Telephone follow-up was carried out in 333 cases of qualified tinnitus outpatients and inpatients from the First Affiliated Hospital of Guangzhou University of Chinese Medicine admitted from January of 2008 to December of 2014. All of the included patients were treated by comprehensive traditional Chinese medical therapy for regulating spleen and stomach. The degree of tinnitus was evaluated by Tinnitus Effect Questionnaire(TEQ) on the first visit and the last visit and during follow-up, and then the TEQ scores were analyzed by statistical methods. Results On the first visit, the mean TEQ scores were 11.17 ± 3.11. On the last visit, the mean treatment course was 33.64 ± 37.07 days, and the mean TEQ scores were 9.57 ± 3.43; 5 cases were cured, 25 were markedly effective, 79 were effective, 224 were ineffective, and the total effective rate was 32.73%. The interval of the first follow-up to the first visit averaged 32.89 ± 8.53 months, and the mean TEQ scores were 5.35 ± 3.71 during the interval; 20 cases were cured, 21 were markedly effective, 39 were effective, 43 were ineffective, and the total effective rate was 65.04%. The interval of the second follow-up to the first visit averaged 57.48 ± 7.91 months, and the mean TEQ scores were 3.42 ± 3.39 during the interval; 37 cases were cured, 21 were markedly effective, 26 were effective, 39 were ineffective, and the total effective rate was 68.29%. Conclusion Comprehensive traditional Chinese medical therapy for regulating spleen and stomach has certain effect in treating tinnitus, and the long-term effect is satisfactory.