Objective To investigate the correlations of serum dual-specific phosphatase 1(DUSP1)and soluble cluster of differentiation 93(sCD93)with severity of disease,lung function and airway inflammation in children with acute-phase bronchial asthma(BA).Methods A total of 100 children with acute-phase BA(acute-phase group),100 children with remission-phase BA(remis-sion-phase group),and 100 healthy children(control group)in the Xingyuan Hospital of Yulin from January 2021 to January 2023 were selected,and the acute-phase group was further divided into mild subgroup(n=47),moderate subgroup(n=36)and severe subgroup(n=17)according to the se-verity of the disease.Serum DUSP1,sCD93 levels and lung function indexes[peak expiratory flow(PEF),percentage of forced expiratory volume in the first second compared to the expected value(FEV1％pred)],and airway inflammation indexes[eosinophil(EOS)count,fractional exhaled nitric oxide(FeNO)]were measured.Pearson's method was used to analyze the correlations of serum DUSP1 and sCD93 levels with PEF,FEV1％pred,EOS and FeNO in children with acute-phase BA.Results The levels of serum DUSP1,PEF,and FEV1％pred in the acute-phase group were signifi-cantly lower than those in the remission-phase group and the control group,while the levels of sCD93,EOS count and FeNO were significantly higher than those in the remission-phase group and the control group(P＜0.01).The levels of serum DUSP1,PEF and FEV1％pred in the severe subgroup were significantly lower than those in the moderate and mild subgroups,while the levels of sCD93,EOS count and FeNO were significantly higher than those in the moderate and mild sub-groups(P＜0.01).PEF and FEV1％pred were positively correlated with serum DUSP1 level,but negatively correlated with sCD93 level in children with acute-phase BA(P＜0.01).EOS count and FeNO were negatively correlated with serum DUSP1 level,but positively correlated with sCD93 level(P＜0.01).Conclusions The decreased serum DUSP1 level and increased sCD93 level in chil-dren with acute-phase BA are closely related to the aggravation of the disease,the decrease of lung function,and airway inflammation,which may become new targets for the treatment of children with acute-phase BA.

Objective To study the anatomical characteristics of the medial sural vessels and the clinical effects of treatment for leg skin and soft tissue defect with free flaps.Methods 6 adult lower limbs were treated by latex perfusion and then observed the origin of the blood supply to gastrocnemius muscle,measure the number of the arteries and veins,the length and diameter of the medial sural vessels.From July 2009 to May 2013,15 clinical cases of serious skin and soft tissue defect were repaired by using free flap with medial sural vessels.The areas of wound surface were ranging from 13 cm × 7 cm-24 cm × 12 cm.10 of them were treated by anterolateral thigh flap,the other of them were treated by latissimus dorsi flap.The donor site were directly sutured or repaired by free skin graft.Results The blood supply of gastrocnemius mucle was multifocal.The medial sural arteries and lateral sural arteries were both origin from popliteal artery.The medial sural vessels include 1 artery and 2 veins.With the length 4-7 cm (average,5.4 cm),the arterial diameter of the origin 2.6-2.9 mm (average,2.7 mm) and the entry point 1.9-2.3 mm (average,2.1 mm),the vein diameter of the origin 1.8-2.2 mm (average,2.0 mm)and the entry point 2.7-3.4 mm(average,2.9 mm).Totally 15 cases flaps were survived with primary healing.The mean follow-up period was 16.5 months(range,11-21 months),The flaps had satisfactory appearance,soft texture,good elasticity and achieved protective sensation at the last followed-up.Conclusions The medial sural vessels are with constant anatomical position,deeply position,hardly damage,long pedicle.Thus,the medial sural vessels combine with free flap is an good choice for the reconstruction of leg skin and soft tissue defect.

Objective To study the anatomical characteristics of the medial sural vessels and the clinical effects of treatment for leg skin and soft tissue defect with free flaps.Methods 6 adult lower limbs were treated by latex perfusion and then observed the origin of the blood supply to gastrocnemius muscle,measure the number of the arteries and veins,the length and diameter of the medial sural vessels.From July 2009 to May 2013,15 clinical cases of serious skin and soft tissue defect were repaired by using free flap with medial sural vessels.The areas of wound surface were ranging from 13 cm × 7 cm-24 cm × 12 cm.10 of them were treated by anterolateral thigh flap,the other of them were treated by latissimus dorsi flap.The donor site were directly sutured or repaired by free skin graft.Results The blood supply of gastrocnemius mucle was multifocal.The medial sural arteries and lateral sural arteries were both origin from popliteal artery.The medial sural vessels include 1 artery and 2 veins.With the length 4-7 cm (average,5.4 cm),the arterial diameter of the origin 2.6-2.9 mm (average,2.7 mm) and the entry point 1.9-2.3 mm (average,2.1 mm),the vein diameter of the origin 1.8-2.2 mm (average,2.0 mm)and the entry point 2.7-3.4 mm(average,2.9 mm).Totally 15 cases flaps were survived with primary healing.The mean follow-up period was 16.5 months(range,11-21 months),The flaps had satisfactory appearance,soft texture,good elasticity and achieved protective sensation at the last followed-up.Conclusions The medial sural vessels are with constant anatomical position,deeply position,hardly damage,long pedicle.Thus,the medial sural vessels combine with free flap is an good choice for the reconstruction of leg skin and soft tissue defect.