[Objective] To validate and optimize the platelet transfusion refractoriness (PTR) prediction model for patients with hematological disorders established by our center. [Methods] The data of patients with hematological diseases who received platelet transfusions from December 2021 to December 2022 were used as the training set, and data from January 2023 to December 2023 as the validation set. The validation set data was used to validate the predictive model constructed on the training set. Relevant risk factors for PTR were collected through literature review and preliminary studies。 The patients were divided into effective and ineffective groups according to the corrected count increment (CCI) of platelet counts. Predictive factors were screened using univariate and multivariate logistic regression. The calibration of the model were assessed via calibration curves, while discrimination, accuracy, sensitivity, and specificity were evaluated using receiver operating characteristic (ROC) curves Clinical utility was further analyzed with decision curve analysis (DCA). [Results] The Hosmer-Lemeshow (H-L) goodness-of-fit test for the validation set yielded S: P=0.000, indicating that the original model needs optimization. Baseline comparisons and logistic regression identified the number of red blood cell units (RBCU) and platelet units (PLT-U) transfused as key predictors for the optimized model. The H-L goodness-of-fit test S: P values for the training and validation sets were 0.930 and 0.056, respectively; the ROC areas were 0.793 5 and 0.809 4, specificities 90.95% and 84.21%, sensitivities 59.26% and 70.04%, and accuracies 78.14% and 74.10%, respectively. DCA demonstrated clinical net benefit within a prediction probability threshold range of 0.2-0.8. [Conclusion] Transfusion volumes of RBC-U and PLT-U were inversely associated with PTR in hematological patients. The resulting PTR prediction model exhibits moderate predictive efficacy and clinical benefit.

Debates persist regarding the efficacy and safety of azvudine, particularly its real-world outcomes. This study involved patients aged ≥60 years who were admitted to 25 hospitals in mainland China with confirmed SARS-CoV-2 infection between December 1, 2022, and February 28, 2023. Efficacy outcomes were all-cause mortality during hospitalization, the proportion of patients discharged with recovery, time to nucleic acid-negative conversion (T _NANC), time to symptom improvement (T _SI), and time of hospital stay (T _HS). Safety was also assessed. Among the 5884 participants identified, 1999 received azvudine, and 1999 matched controls were included after exclusion and propensity score matching. Azvudine recipients exhibited lower all-cause mortality compared with controls in the overall population (13.3% vs. 17.1%, RR, 0.78; 95% CI, 0.67-0.90; P = 0.001) and in the severe subgroup (25.7% vs. 33.7%; RR, 0.76; 95% CI, 0.66-0.88; P < 0.001). A higher proportion of patients discharged with recovery, and a shorter T _NANC were associated with azvudine recipients, especially in the severe subgroup. The incidence of adverse events in azvudine recipients was comparable to that in the control group (2.3% vs. 1.7%, P = 0.170). In conclusion, azvudine showed efficacy and safety in older patients hospitalized with COVID-19 during the SARS-CoV-2 omicron wave in China.

Objective Exploring the effectiveness of blended learning based on the BOPPPS model applied to the education of medical students in professionalism.Methods A total of 96 clinical medical students in the 2023 grade of Harbin Medical University were selected as the research subjects.The control group was taught using traditional teaching methods,while the experimental group was taught using blended teaching methods based on the BOPPPS model.The teaching effectiveness of the two groups was compared using scale assessment and process evaluation methods.Results The experimental group scored significantly higher than the control group in terms of theoretical knowledge,professional awareness,professional ethics,communication skills,teamwork,and other indicators(P＜0.05).Conclusion The BOPPPS-based teaching model has shown significant application effects in the education of professionalism for medical students,which can effectively promote the cultivation of professionalism for medical students.

Objective Exploring the effectiveness of blended learning based on the BOPPPS model applied to the education of medical students in professionalism.Methods A total of 96 clinical medical students in the 2023 grade of Harbin Medical University were selected as the research subjects.The control group was taught using traditional teaching methods,while the experimental group was taught using blended teaching methods based on the BOPPPS model.The teaching effectiveness of the two groups was compared using scale assessment and process evaluation methods.Results The experimental group scored significantly higher than the control group in terms of theoretical knowledge,professional awareness,professional ethics,communication skills,teamwork,and other indicators(P＜0.05).Conclusion The BOPPPS-based teaching model has shown significant application effects in the education of professionalism for medical students,which can effectively promote the cultivation of professionalism for medical students.

Objective To explore the risk factors for platelet transfusion refractoriness(PTR)in patients with hemato-logical disorders,construct a prediction model and validate the model efficacy.Methods Patients with hematological disor-ders who received platelet transfusion therapy in the Chengdu Second People's Hospital from December 2021 to December 2022 were retrospectively included to judge the effectiveness of platelet transfusion and screened for risk factors by univariate and multivariate logistic regression.A prediction model for PTR was constructed using receiver operating characteristic(ROC)curve,calibration curve and decision curve(DCA)to assess the differentiation,calibration and clinical value of the model,respectively.Results A total of 334 hematological patients were included,including 168 males and 176 females,with a PTR incidence of 40.4%.Univariate and multivariate logistic regression analysis showed that platelet transfusion vol-ume,erythrocyte transfusion volume,and neutrophil ratio were risk factors for PTR(P<0.05).A prediction model for PTR in hematological patients was established based on these risk factors.The area under the model's curve was 0.8377(95%CI:0.723-0.772),the sensitivity was 58.52%,and the specificity was 89.95%.The calibration curve showed that the S∶P was 0.964,the maximum absolute difference Emax was 0.032,and the average absolute difference Eavg was 0.009.The DCA a-nalysis showed that the model had clinical application value when the risk threshold ranged from 0.2 to 0.9.Conclusion The PTR prediction model based on platelet transfusion volume,erythrocyte transfusion volume and neutrophil ratio can pro-vide a basis for effective platelet transfusion in hematological patients.

Objective Exploring the effectiveness of blended learning based on the BOPPPS model applied to the education of medical students in professionalism.Methods A total of 96 clinical medical students in the 2023 grade of Harbin Medical University were selected as the research subjects.The control group was taught using traditional teaching methods,while the experimental group was taught using blended teaching methods based on the BOPPPS model.The teaching effectiveness of the two groups was compared using scale assessment and process evaluation methods.Results The experimental group scored significantly higher than the control group in terms of theoretical knowledge,professional awareness,professional ethics,communication skills,teamwork,and other indicators(P＜0.05).Conclusion The BOPPPS-based teaching model has shown significant application effects in the education of professionalism for medical students,which can effectively promote the cultivation of professionalism for medical students.

Objective Exploring the effectiveness of blended learning based on the BOPPPS model applied to the education of medical students in professionalism.Methods A total of 96 clinical medical students in the 2023 grade of Harbin Medical University were selected as the research subjects.The control group was taught using traditional teaching methods,while the experimental group was taught using blended teaching methods based on the BOPPPS model.The teaching effectiveness of the two groups was compared using scale assessment and process evaluation methods.Results The experimental group scored significantly higher than the control group in terms of theoretical knowledge,professional awareness,professional ethics,communication skills,teamwork,and other indicators(P＜0.05).Conclusion The BOPPPS-based teaching model has shown significant application effects in the education of professionalism for medical students,which can effectively promote the cultivation of professionalism for medical students.

Objective Exploring the effectiveness of blended learning based on the BOPPPS model applied to the education of medical students in professionalism.Methods A total of 96 clinical medical students in the 2023 grade of Harbin Medical University were selected as the research subjects.The control group was taught using traditional teaching methods,while the experimental group was taught using blended teaching methods based on the BOPPPS model.The teaching effectiveness of the two groups was compared using scale assessment and process evaluation methods.Results The experimental group scored significantly higher than the control group in terms of theoretical knowledge,professional awareness,professional ethics,communication skills,teamwork,and other indicators(P＜0.05).Conclusion The BOPPPS-based teaching model has shown significant application effects in the education of professionalism for medical students,which can effectively promote the cultivation of professionalism for medical students.

Objective Exploring the effectiveness of blended learning based on the BOPPPS model applied to the education of medical students in professionalism.Methods A total of 96 clinical medical students in the 2023 grade of Harbin Medical University were selected as the research subjects.The control group was taught using traditional teaching methods,while the experimental group was taught using blended teaching methods based on the BOPPPS model.The teaching effectiveness of the two groups was compared using scale assessment and process evaluation methods.Results The experimental group scored significantly higher than the control group in terms of theoretical knowledge,professional awareness,professional ethics,communication skills,teamwork,and other indicators(P＜0.05).Conclusion The BOPPPS-based teaching model has shown significant application effects in the education of professionalism for medical students,which can effectively promote the cultivation of professionalism for medical students.

AIM:To investigate the impact of aquaporin 4(AQP4)expression inhibition on autophagy and apoptosis in a mouse model of cerebral ischemia-reperfusion(I/R)injury,and to elucidate its underlying mechanism.METHODS:Cerebral I/R injury was induced in mice via transient middle cerebral artery occlusion(tMCAO).Totally 60 mice were randomly divided into sham group,I/R group,AQP4 inhibition group,and 3-methyladenine(3-MA)group,with 15 mice in each group.Among them,the mice in sham and I/R groups received intraperitoneal injections of normal saline,while those in AQP4 inhibition group and 3-MA group received intraperitoneal injections of AER-271(2 mg·kg-1·d-1)and AER-271+3-MA(2 mg·kg-1·d-1)for 3 d,respectively,once per day.Longa score was adopted to assess the neu-rological function,and to record changes in body weight.Cerebral infarction volume and histopathological alterations were evaluated using hematoxylin-eosin staining.Western blot analysis was performed to determine the levels of AQP4,LC3-Ⅱ,P62 and cleaved caspase-3,while the LC3-Ⅱ,P62,cleaved caspase-3 and NeuN(neuronal marker)colocalization and expression assessment were conducted with immunofluorescence.RESULTS:The mice in I/R and AQP4 inhibition groups exhibited extensive cerebral infarction,cerebral edema,and elevated Longa scores.However,in comparision to I/R group,the mice in AQP4 inhibition group showed significantly reduced cerebral infarct volume,cerebral edema vol-ume,and Longa score(P<0.05).Additionally,in contrast to sham group,the mice in I/R group displayed increased ex-pression of AQP4,LC3-Ⅱ and cleaved caspase-3(P<0.01),accompanied by decreased body weight and P62 expression(P<0.05 or P<0.01).Furthermore,compared with I/R group,the mice in both AQP4 inhibition group and 3-MA group demonstrated a decrease in the expression levels of AQP4,LC3-Ⅱ and cleaved caspase-3(P<0.05 or P<0.01),along with increased body weight and P62 expression(P<0.05 or P<0.01).Nonetheless,no significant differences were ob-served between AQP4 inhibition group and 3-MA group regarding Longa score,cerebral infarct volume,body weight,and the expression of AQP4,LC3-Ⅱ,cleaved caspase-3 and P62.CONCLUSION:Inhibition of AQP4 expression signifi-cantly reduces cerebral infarction area and nerve injury severity in tMCAO mice.Moreover,AQP4 expression inhibition decelerates autophagy and apoptosis after cerebral infarction,with the additional autophagy inhibitor showing no notable impact on the protective effect of AQP4 inhibition.