Objective To investigate the expression of Delta(24)-cholesteryl reductase(DHCR24)proteins in cervical squamous intraepithelial lesions(SILs)tissues and its value in different cervical lesion patho-logical diagnosis.Methods The expression of DHCR24,p16,and Ki-67 was quantitatively detected by immuno-histochemistry in 51 normal cervical tissues,44 LSILs,and 57 HSILs.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic efficacy of DHCR24,p16,and Ki-67 proteins in evaluating the degree of SILs.Results The expression levels of DHCR24,p16 and Ki-67 protein were positively correlated with the progression of SILs(P<0.05).ROC analysis showed that the immunohistochemistry score cutoff value for DHCR24 between normal cervical tissue and LSIL was 0.1145,and between LSIL and HSIL was 0.1969.The sensi-tivity of DHCR24 in diagnosing LSIL was 79.55%,higher than that for p16 and Ki-67,which was 15.91%and 18.08%(P<0.05).The area under the ROC curve(AUC)for distinguishing normal cervical tissue from LSIL using a combination of DHCR24 and p16 was 0.932(95%CI:0.878～0.986),higher than that for p16 and Ki-67 combined,which was 0.861(95%CI:0.785～0.936).The AUC for distinguishing LSIL from HSIL using a combi-nation of DHCR24 and p16 was0.971(95%CI:0.946～0.997),higher than that for p16 and Ki-67 combined,which was 0.870(95%CI:0.790～0.949).Conclusions Both DHCR24 and p16 protein expression levels can pro-vide reference for the grading of SILs,and their combination can improve the diagnostic efficiency.The cutoff value derived from the ROC curve plotted by DHCR24 immunohistochemical staining intensity can improve the sensitivity of LSIL diagnosis.

Objective To investigate the expression of Delta(24)-cholesteryl reductase(DHCR24)proteins in cervical squamous intraepithelial lesions(SILs)tissues and its value in different cervical lesion patho-logical diagnosis.Methods The expression of DHCR24,p16,and Ki-67 was quantitatively detected by immuno-histochemistry in 51 normal cervical tissues,44 LSILs,and 57 HSILs.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic efficacy of DHCR24,p16,and Ki-67 proteins in evaluating the degree of SILs.Results The expression levels of DHCR24,p16 and Ki-67 protein were positively correlated with the progression of SILs(P<0.05).ROC analysis showed that the immunohistochemistry score cutoff value for DHCR24 between normal cervical tissue and LSIL was 0.1145,and between LSIL and HSIL was 0.1969.The sensi-tivity of DHCR24 in diagnosing LSIL was 79.55%,higher than that for p16 and Ki-67,which was 15.91%and 18.08%(P<0.05).The area under the ROC curve(AUC)for distinguishing normal cervical tissue from LSIL using a combination of DHCR24 and p16 was 0.932(95%CI:0.878～0.986),higher than that for p16 and Ki-67 combined,which was 0.861(95%CI:0.785～0.936).The AUC for distinguishing LSIL from HSIL using a combi-nation of DHCR24 and p16 was0.971(95%CI:0.946～0.997),higher than that for p16 and Ki-67 combined,which was 0.870(95%CI:0.790～0.949).Conclusions Both DHCR24 and p16 protein expression levels can pro-vide reference for the grading of SILs,and their combination can improve the diagnostic efficiency.The cutoff value derived from the ROC curve plotted by DHCR24 immunohistochemical staining intensity can improve the sensitivity of LSIL diagnosis.

OBJECTIVE： To investigate the correlation of ADPRT rs1136410 polymorphism with the occurrence of non-small cell lung cancer （NSCLC） in Han nationality from northern Jiangsu. METHODS： A total of 283 patients with primary NSCLC of Han nationality in Northern Jiangsu were selected from the Affiliated Hospital of Xuzhou Medical University during Nov. 2015-Dec. 2018 as NSCLC group. A total of 210 healthy subjects underwent physical examination were included in control group. PCR-RFLP was utilized to determine the genotypes at ADPRT rs1136410 locus. Logistic regression model was used to evaluate the effect of polymorphism and its interaction with smoking on the occurrence of NSCLC. RESULTS： There was no statistical significance in age and gender between 2 groups （P＞0.05）. The proportion of smoker in NSCLC group was significantly higher than control group （P＜0.05）. TT， TC and CC genotypes were detected at rs1136410 locus of ADPRT gene. The frequency of TT， TC and CC genotype were 41.9％，44.8％ and 13.3％， and those of allele T and C were 64.3％ and 35.7％ in control group. The frequency of TT， TC and CC genotype were 21.6％， 50.2％ and 28.2％， and those of allele T and C were 46.6％ and 53.4％ in NSCLC group， respectively. The frequencies of genotypes in 2 groups were in accordance with Hardy-Weinberg equilibrium （P＞0.05）， while there was significant difference in genotype and allele frequencies between 2 groups （P＜0.05）. Compared with TT genotype， the risk of NSCLC in individuals carrying TC and CC genotypes raised by 1.179， 3.122 folds [ORTC＝2.179， 95％CI （1.435， 3.309）， P＜0.05； ORCC＝4.122，95％CI（2.401，7.075），P＜0.05]. Compared with individuals carrying TT genotype， the risk of NSCLC occurrence in non-smokers carrying TC and CC genotypes increased by 0.371， 1.328 fold [ORTC＝1.371，95％CI （0.927，3.428），P＜0.05； ORCC＝2.328，95％CI （1.249，4.622），P＜0.05]； and the risk of NSCLC occurrence in smokers carrying TC and CC genotypes increased by 0.928， 2.182 folds [ORTC＝1.928，95％CI （1.257，2.957）， P＜0.05；ORCC＝3.182，95％CI （1.760，5.754）， P＜0.05]. CONCLUSIONS： The rs1136410 locus mutant genotype of ADPRT gene is the risk factor of NSCLC in Han nationality from Northern Jiangsu， and smoking raises this risk of NSCLC occurrence in individuals with mutation genotypes of ADPRT rs1136410.