This research aimed to comprehensively understand the prevalence of mutation in drug-resistant molecular markers of imported Plasmodium vivax in Chongqing,the Pvmdr1,Pvdhps,Pvdhfr,Pvcrt-o and Pvk12 genes of Plasmodium vivax were systematically analyzed.Blood samples were collected from imported Plasmodium vivax-infected patients in Chongqing between 2011 and 2022.The Pvmdr1,Pvdhps,Pvdhfr,Pvcrt-o,and Pvk12 genes were amplified and then sequenced to precisely evaluate gene mutations.Bioinformatics methods were employed to conduct in depth analysis of the mutation prevalence.Regarding the Pvdhfr gene,mutations at codons 50,57,58,61,99,117,and 199 were detected in 2.9%,23.5%,76.4%,23.53%,2.9%,82.3%,and 5.88%of the samples,respectively.The double-mutant haplotype S58R/S117N was the most prevalent,accounting for 50%,followed by the quadruple-mutant haplotype F57L/S58R/T61M/S117T,which accounted for 11.76%.Among the four types of tandem-repeat variations of Pvdhfr,the wild-type was the most common,and the insertion type was a novel discovery in this study.For the Pvdhps gene,the prevalence among mutation genotypes was relatively low.The single-mutant genotype was dominant,constituting 27.03%.The prevalence of Pvmdr1 mutations at codons 958 and 1076 was 100%and 89.19%,respectively.Among the 37 successfully sequenced samples,K10 insertion was detected in only 8 cases(22.22%).Notably,no non-synonymous mutations of Pvk12 were identified in this study.The cases in this study were imported from various countries of origin.Novel tandem-repeat variation tyres of Pvdhfr and new mutation sites of Pvdhps were identifide,thus enriching the mutation information of imported Plasmodium vivax resistance molecular markers in China.

In response to existing issues in preventive medicine education in China, Chongqing Medical University has implemented a series of reform measures in the major of preventive medicine since 2017. The construction of national first-class major of preventive medicine adopted the concept of "student-centered and market-oriented". This major scientifically formulated the talent cultivation goal of "cultivating high-quality, innovative, and application-oriented talents", constructed a curriculum system centered around professional competency training, emphasized a teaching mode that integrated "digital intelligence empowerment" and "combination of theory and practice". Moreover, we aimed to advance the ideological and political education reform of the courses, refine the diversified evaluation system, and bolster the development of teaching staff and the assurance of teaching quality. Following these reforms, the major has made significant achievements in talent cultivation effectiveness, curriculum system development, and teaching mode innovation. In the future, the major will persist in deepening the digital intelligence transformation, fostering interdisciplinary integration, enhancing international cooperation, and continuously advancing the construction and reform of preventive medicine as a national first-class major.

New Medical Sciences, as a crucial initiative in transforming and upgrading medical education and healthcare services in China, has promoted deep integration of public health with multiple disciplines. However, the public health discipline still faces numerous challenges such as disease prevention system reconstruction, insufficient technological innovation, and the shortage of professional talents. To address these issues, this paper discusses the current status, challenges, and development of the public health discipline in the context of New Medical Sciences, and proposes strategies including reforming talent cultivation models, enhancing practical capabilities, strengthening faculty development, promoting the innovation of scientific research and social service, actively utilizing artificial intelligence technology, and optimizing international cooperation. These measures aim to achieve innovative development in the public health discipline, and better serve the Healthy China strategy and global public health initiatives.

This research aimed to comprehensively understand the prevalence of mutation in drug-resistant molecular markers of imported Plasmodium vivax in Chongqing,the Pvmdr1,Pvdhps,Pvdhfr,Pvcrt-o and Pvk12 genes of Plasmodium vivax were systematically analyzed.Blood samples were collected from imported Plasmodium vivax-infected patients in Chongqing between 2011 and 2022.The Pvmdr1,Pvdhps,Pvdhfr,Pvcrt-o,and Pvk12 genes were amplified and then sequenced to precisely evaluate gene mutations.Bioinformatics methods were employed to conduct in depth analysis of the mutation prevalence.Regarding the Pvdhfr gene,mutations at codons 50,57,58,61,99,117,and 199 were detected in 2.9%,23.5%,76.4%,23.53%,2.9%,82.3%,and 5.88%of the samples,respectively.The double-mutant haplotype S58R/S117N was the most prevalent,accounting for 50%,followed by the quadruple-mutant haplotype F57L/S58R/T61M/S117T,which accounted for 11.76%.Among the four types of tandem-repeat variations of Pvdhfr,the wild-type was the most common,and the insertion type was a novel discovery in this study.For the Pvdhps gene,the prevalence among mutation genotypes was relatively low.The single-mutant genotype was dominant,constituting 27.03%.The prevalence of Pvmdr1 mutations at codons 958 and 1076 was 100%and 89.19%,respectively.Among the 37 successfully sequenced samples,K10 insertion was detected in only 8 cases(22.22%).Notably,no non-synonymous mutations of Pvk12 were identified in this study.The cases in this study were imported from various countries of origin.Novel tandem-repeat variation tyres of Pvdhfr and new mutation sites of Pvdhps were identifide,thus enriching the mutation information of imported Plasmodium vivax resistance molecular markers in China.

New Medical Sciences, as a crucial initiative in transforming and upgrading medical education and healthcare services in China, has promoted deep integration of public health with multiple disciplines. However, the public health discipline still faces numerous challenges such as disease prevention system reconstruction, insufficient technological innovation, and the shortage of professional talents. To address these issues, this paper discusses the current status, challenges, and development of the public health discipline in the context of New Medical Sciences, and proposes strategies including reforming talent cultivation models, enhancing practical capabilities, strengthening faculty development, promoting the innovation of scientific research and social service, actively utilizing artificial intelligence technology, and optimizing international cooperation. These measures aim to achieve innovative development in the public health discipline, and better serve the Healthy China strategy and global public health initiatives.

In response to existing issues in preventive medicine education in China, Chongqing Medical University has implemented a series of reform measures in the major of preventive medicine since 2017. The construction of national first-class major of preventive medicine adopted the concept of "student-centered and market-oriented". This major scientifically formulated the talent cultivation goal of "cultivating high-quality, innovative, and application-oriented talents", constructed a curriculum system centered around professional competency training, emphasized a teaching mode that integrated "digital intelligence empowerment" and "combination of theory and practice". Moreover, we aimed to advance the ideological and political education reform of the courses, refine the diversified evaluation system, and bolster the development of teaching staff and the assurance of teaching quality. Following these reforms, the major has made significant achievements in talent cultivation effectiveness, curriculum system development, and teaching mode innovation. In the future, the major will persist in deepening the digital intelligence transformation, fostering interdisciplinary integration, enhancing international cooperation, and continuously advancing the construction and reform of preventive medicine as a national first-class major.

Objective To explore the effects of occupational noise exposure on hypertension in Chongqing area,and to provide reference for the prevention and treatment of hypertension in noise-exposed populations.Methods Using the Chongqing key occupational disease surveillance and occupational disease hazard factor site monitoring database,the samples of adult male and female occupational noise-exposed popu-lations in Chongqing were selected by the cluster sampling,the physical examination data were collected,and the degree of hearing loss was evaluated by the binaural high-frequency mean hearing thresholds.The follow-up lasted for 3 years (2017-2019) and 5 years (2017-2021).The age,noise operation working age,BMI and blood pressure were respectively compared among the male and female respondents with different degrees of hearing loss;the Cox proportional risk regression model was adopted to analyze the relationship between de-gree of hearing loss and hypertension.Results After 3-year follow-up,the prevalence rate of hypertension in the respondents was 6.47％(308/4761),6.92％(289/4178) in males and 3.26％(19/583) in females;the de-tection rates of normal hearing,mild hearing loss and high degree of hearing loss were 46.42％,42.62％ and 10.96％ respectively.After 5-year follow-up,the prevalence rate of hypertension among the respondents was 12.44％ (431/3464),12.92％(399/3088) in males,and 8.51％(32/376) in females;the detection rates of normal hearing,mild hearing loss,and high degree of hearing loss were 64.20％,25.87％ and 9.93％ respec-tively.After 3-year,5-year follow-up,there was no significant difference in the systolic blood pressure among respondents with different degrees of hearing loss in males and females (P>0.05),but the diastolic blood pressure had significant difference (P<0.05).After adjusting the confounding factors,the 5-year risk of hy-pertension in male respondents with noise-induced mild hearing loss (binaural high-frequency mean hearing threshold 25-40 dB) was significantly increased (HR=1.25,95％CI:1.05-1.48,P<0.01).The 5-year risk of hypertension in female respondents with noise induced high degree of hearing loss (binaural high-frequency mean hearing threshold>40 dB) was significantly increased (HR=1.26,95％CI:1.10-1.44,P<0.01). Conclusion Among the populations with occupational noise exposure,the risk of developing hypertension in males with mild hearing loss is increased,and the risk of developing hypertension in males with high degree of hearing loss is increased.

Children with pediatric tumors have better prognosis and longer survival than adults, suggesting that attention should be paid to the long-term complications induced by radiotherapy. In this article, the data from more than 40 clinical studies of pediatric tumor radiotherapy published in the recent decade were retrospectively analyzed. Long-term complications of nervous system, cardio-cerebrovascular system, respiratory system, endocrine system, urinary system, reproductive system, skeletal development, long-term secondary tumors were considered and the corresponding radiation dose-volume parameters were summarized, aiming to guide radiation oncology physicians and physicists to optimize radiotherapy plans for children with pediatric tumors.

BACKGROUND: We aim to explore the association between self-reported snoring and hypertension among adults aged 30-79 in Chongqing, China. METHODS: A total of 23,342 individuals aged 30-79 were included at baseline from August 2018 to January 2019, and the final sample size for the analysis was 22,423. Face-to-face interviews and physical examinations were conducted by trained investigators. Logistic regression was performed to study age-specific and gender-specific associations between snoring and hypertension. RESULTS: Frequent snoring was associated with the risk of hypertension for each age and gender group, and the frequency of snoring was positively correlated with the risk for hypertension. For the three age groups (< 45, 45-59, ≥ 60), compared with the non-snoring group, those who snore often had a 64.5%, 53.3%, and 24.5% increased risk of hypertension (< 45: OR = 1.65, 95%CI 1.34-2.02; 45-59: OR = 1.53, 95%CI 1.37-1.72; ≥ 60: OR = 1.25, 95%CI 1.09-1.42), respectively. For men and women, those who snore often had a 46.8% and 97.2% increased risk of hypertension, respectively, than the non-snoring group (men: OR = 1.47, 95%CI 1.33-1.63; women: OR = 1.97, 95%CI 1.75-2.23). CONCLUSIONS People who snore frequently should pay close attention to their blood pressure levels in order to achieve early prevention of hypertension, particularly for snorers who are female and aged under 45; importance should be attached to their blood pressure control.
Adult ; Aged ; China/epidemiology* ; Cross-Sectional Studies ; Female ; Humans ; Hypertension/etiology* ; Male ; Middle Aged ; Self Report ; Snoring/complications*

Objective:To understand the current situation of medical humanistic quality of standardized trainees for hospitalizations in Chongqing, and put forward the corresponding educational countermeasures and suggestions. Methods: According to the method of convenient sampling, 5 resident doctors standardized training base were selected with half a random, then 110 trainees were randomly selected to conduct a cognition investigation about the medical humanistic quality. EpiData 3. 0 was adopted for double entry data and SPSS22. 0 software was used for analyzing data. Results:There were 98. 06％ of standardized trainees for hospitalizations paid more attention to medical humanistic quality. In the selection of medical humanistic quality courses, 81. 55％ of the trainees chose The Doctor-patient Relationship, 77. 67％ of the trainees chose The Medical Psychology. In the evaluation of the importance of medical humanistic quality to the clinical work, 96. 12％ of the standardized trainees for hospitalizations believed that it was important. Conclusion: In order to improve the medical humanistic quality of the standardized trainees for hospitalizations, all aspects should be taken seriously:it should make efforts from the trai-nees' self-awareness, the teaching teachers' attention to the medical humanistic quality and the medical humanis-tic quality environment provided by the training base.