Abstract Nutritional literacy is an important component of health literacy and closely related to adolescents dietary habits and health conditions. Improving nutrition literacy not only helps adolescents to make healthier dietary choices but also aids in disease prevention. The article systematically reviews the individual and environmental factors influencing adolescent nutrition literacy, with a focus on exploring innovative intervention strategies based on traditional school interventions, new media platforms and virtual reality technology, so as to provide a theoretical foundation and practical guidance for improving the nutrition literacy and overall health of Chinese adolescents.

Objective:To investigate the reproductive health knowledge, contraceptive attitudes and behaviors of male partners of abortion women and their sources of reproductive health knowledge, and to analyze the correlation among them.Methods:A questionnaire survey was conducted to survey 1085 female's male partners who requested abortion in Shanghai General Hospital, and information on male reproductive health knowledge, knowledge sources, contraceptive attitudes and implementation was collected, and the results were analyzed.Results:"Network" (78.52%, P<0.001) was the most important way for males with various sociological characteristics to acquire reproductive health knowledge, followed by "friends, colleagues" (36.76%), "medical staff" (34.26%), "TV video" (33.80%), and "parental elders" (15.83%) which accounted for the lowest proportion. The more ways for abortion female's male partners to acquire reproductive health knowledge, the higher level of reproductive health knowledge such as contraception and abortion ( P=0.001), the higher active contraceptive attitude (91.49%, P<0.001). Conclusion:The main ways for male to obtain reproductive health knowledge include the internet, healthcare workers, media publicity, etc. and the more access, the more abundant the reproductive health knowledge of men, the more likely they are to take effective contraceptive measures and reduce the incidence of unintended pregnancy abortion.

Objective:To investigate the reproductive health knowledge, contraceptive attitudes and behaviors of male partners of abortion women and their sources of reproductive health knowledge, and to analyze the correlation among them.Methods:A questionnaire survey was conducted to survey 1085 female's male partners who requested abortion in Shanghai General Hospital, and information on male reproductive health knowledge, knowledge sources, contraceptive attitudes and implementation was collected, and the results were analyzed.Results:"Network" (78.52%, P<0.001) was the most important way for males with various sociological characteristics to acquire reproductive health knowledge, followed by "friends, colleagues" (36.76%), "medical staff" (34.26%), "TV video" (33.80%), and "parental elders" (15.83%) which accounted for the lowest proportion. The more ways for abortion female's male partners to acquire reproductive health knowledge, the higher level of reproductive health knowledge such as contraception and abortion ( P=0.001), the higher active contraceptive attitude (91.49%, P<0.001). Conclusion:The main ways for male to obtain reproductive health knowledge include the internet, healthcare workers, media publicity, etc. and the more access, the more abundant the reproductive health knowledge of men, the more likely they are to take effective contraceptive measures and reduce the incidence of unintended pregnancy abortion.

Arylalkylamine N-acetyltransferase (AANAT) and Hydroxyindole O-methyltransferase(HIOMT) are the key regulation enzymes in the melatonin biosynthesis pathway in mammals. The AANAT and HIOMT genes were constructed into a binary plant expression vector YXu55. Using leaf strips as the recipiences, we efficiently transformed tobacco (Nicotiana tabacum) variety qinyan 95 by the Agrobacterium mediated method. After gradient selection with gentamycin, a number of transgenic plants were regenerated. Southern blot and RT-PCR analyses showed that the AANAT-HIOMT genes were integrated into the genome of the transgenic plants and the target genes could express at the level of RNA transcription. By RP-HPLC, we measured the melatonin contents in transgenic plants. The results showed that the melatonin level in YXu55 (containing the gentamycin-resistance gene, the AANAT gene and HIOMT gene) transgenic plants were much higher than those in pZP122 (control containing only the gentamycin-resistance gene) transgenic plants and nontransgenic plants. The content of melatonin in pZP122 transgenic plants was nearly the same as that in nontransgenic plants. Physiological determination of antioxidative characteristics demonstrated that 1) the capacity of total antioxidation, 2) the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and 3) the content of glutathione (GSH) were increased in YXu55 transgenic plants containing the AANAT-HIOMT genes as compared to the control plants (pZP122 or nontransgenic plants). At the same time, malonaldehyde (MDA) content did not appear remarkably difference between transgenic plants and nontransgenic plants. The above mentioned facts indicate enhancement of melatonin levels in YXu55 transgenic plants might help to reduce damage by oxidative stress.
Acetylserotonin O-Methyltransferase ; genetics ; Agrobacterium tumefaciens ; genetics ; Arylalkylamine N-Acetyltransferase ; genetics ; Catalase ; metabolism ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Melatonin ; biosynthesis ; Peroxidase ; metabolism ; Plants, Genetically Modified ; enzymology ; genetics ; Superoxide Dismutase ; metabolism ; Tobacco ; enzymology ; genetics ; Transduction, Genetic ; methods

Objective To determine the content of polysaccharide of prepared pinellia tuber. Methods The content was determined by sulfuric acid-anthrone colorimetric method with wavelength at (624?1)nm. Results The content of polysaccharide was 2.533 mg/g in prepared pinellia tuber. The average recovery was 99.54% and RSD was 1.69%. Conclusion The methods was simple, steady and reproducible.