OBJECTIVE To provide a reference for the optimization of prescription-review software and the selection of auxiliary prescription-review tools for medical institutions.METHODS A total of 559 eligible prescriptions were collected from the Meikang Prescription Automatic Screening System of a tertiary grade-A hospital from January 2025 to March 2026. Judged according to the composite reference standard （CRS） established in this study， there were 206 positive prescriptions [with drug-drug interaction （DDI）] and 353 negative prescriptions （without DDI）. DeepSeek， Doubao， Wenxin yiyan， Kimi and Tongyi qianwen were separately adopted to identify DDI in the 559 prescriptions， and binary judgment was performed on their output texts related to DDI. Taking the results judged by CRS as the gold standard， the confusion matrix was constructed and model performance indicators were calculated. The differences in sensitivity between any two large language models were compared， the relative sensitivity （RS） and its 95% confidence interval （CI） were calculated， and the results were visualized by a forest plot.RESULTS Wenxin yiyan achieved the highest sensitivity （74.8%） with the lowest risk of missed reports， whereas its specificity was only 40.8%. It had the lowest accuracy （53.3%） and presented a high risk of false positive reports. Tongyi qianwen obtained the highest accuracy （65.3%） and the optimal F1-score （0.587）. DeepSeek showed moderate performance in both sensitivity and specificity. Doubao and Kimi exhibited relatively low sensitivity （38.8% and 45.1%， respectively）， accompanied by high risks of missed reports. Pairwise comparison revealed that the sensitivity of Wenxin yiyan was significantly higher than that of DeepSeek [ χ 2 ＝4.84， P ＝0.028； RS＝1.124， 95%CI （1.018， 1.230）]. The sensitivity of Tongyi qianwen was significantly higher than that of Doubao [ χ 2 ＝4.77， P ＝0.029； RS＝1.239， 95%CI （1.035， 1.443）]. DeepSeek had significantly higher sensitivity than Doubao [ χ 2 ＝37.65， P ＜0.001； RS＝1.715， 95%CI （1.491， 1.939）]. No statistically significant differences in sensitivity were observed between Kimi versus Doubao and between Tongyi qianwen versus Kimi （ P ＞0.05）.CONCLUSIONS Wenxin yiyan is applicable for preliminary-screening scenarios with low tolerance for missed reports and requires combined manual review. Tongyi qianwen is suitable for auxiliary outpatient prescription review. DeepSeek can act as a transitional solution for medical institutions without dedicated prescription-review software. Doubao and Kimi are not recommended for independent application in prescription DDI audit.

Atherosclerosis, a chronic inflammatory disease, is markedly influenced by both immune and inflammatory reactions throughout its progression. Dendritic cells, as pivotal antigen-presenting entities, play a crucial role in the initiation of immune responses and the preservation of immunological homeostasis. Accumulating data indicates that dendritic cells are present in healthy arteries and accumulate significantly in atherosclerotic plaques. Novel immunotherapeutic approaches and vaccination protocols have yielded substantial clinical advancements in managing chronic inflammatory diseases, with dendritic cell-centric modalities emerging for atherosclerotic management. In this review, we delineate the essential functions and underlying mechanisms of dendritic cells and their subsets in the modulation of atherosclerotic inflammation and immune responses. We underscore the immense promise of dendritic cell-based immunotherapeutic strategies, including vaccines and innovative combinations with nanotechnological drug delivery platforms for atherosclerosis treatment. We also discuss the challenges associated with dendritic cell immunotherapy and provide perspectives on the future direction of this field.

Objective To analyze the changes in optical coherence tomography angiography (OCTA) parameters in patients with essential hypertension，and to explore the effect of blood pressure on OCTA parameters. Methods A total of 164 patients with essential hypertension were selected and divided into controlled blood pressure group (n=92) and uncontrolled blood pressure group (n=72). OCTA examination was performed on the optic disc and macula of all patients, and the right eyes were selected for analysis. Results There were no significant differences in retinal nerve fiber layer (RNFL) thickness, radial peripapillary capillary (RPC) total vascular density, RPC total small vessel density, perifovea superficial capillary plexus (SCP) vascular density, and perifovea deep capillary plexus (DCP) vascular density between the two groups of patients. There were no significant differences in foveal avascular zone (FAZ) area, FAZ diameter, and fovea retinal thickness between the two groups of patients. The density of the parafovea SCP, parafovea DCP, and fractal dimension (FD) in the uncontrolled blood pressure group were significantly lower than those in the controlled blood pressure group (P＜0.05). Multiple linear regression analysis showed that elevation of blood pressure was a independently related factor of reduced parafovea DCP density (P＝0.026), while there was no correlation between the uncontrolled blood pressure and parafovea SCP density and FD level. Conclusions The blood pressure level is correlated with the parafovea DCP density, while has no correlation with other OCTA parameters in hypertension patients.

Objective:To construct three photosensitive fusion proteins between protein G C3 domain and Avitag for site-spe-cific biotinylation of goat IgG.Methods:DNA sequences of protein G C3 domain with amber codon mutation and C-terminal containing 1,2,3 Avitag were obtained by chemical synthesis,respectively,and sub-cloned into pTXB1 plasmid to construct three fusion pro-tein expression vectors.Three plasmids and pEVOL-pBpF plasmid were co-transformed into competent bacterium E.coli BL21,respec-tively.Target fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were prepared using genetic codon extension technology,and were purified by His Trap Chelating HP column,then biotinylated by Avitag/BirA system.Photo-conjugation efficiency,crosslink site and antigen-binding activity of photo-conjugates were investigated by SDS-PAGE,control experiment,and competitive ELISA.Results:Three expression vectors were successfully constructed,and three designed photoactivatable fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were successfully expressed with molecular weights consistent with their theoretical values.Heavy-chain conjugation efficiencies of biotinylated fusion protein with goat IgG were 85%with Fc-specific conjugation,and conjugates maintained its antigen-binding activity after photo-conjugation.Conclusion:CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 fusion proteins are successfully prepared,and mediates by those photosensitive fusion proteins,biotins molecules are conjugated to Fc fragment of goat IgG,results site-specific biotinylation of goat IgG.

Objective:To construct three photosensitive fusion proteins between protein G C3 domain and Avitag for site-spe-cific biotinylation of goat IgG.Methods:DNA sequences of protein G C3 domain with amber codon mutation and C-terminal containing 1,2,3 Avitag were obtained by chemical synthesis,respectively,and sub-cloned into pTXB1 plasmid to construct three fusion pro-tein expression vectors.Three plasmids and pEVOL-pBpF plasmid were co-transformed into competent bacterium E.coli BL21,respec-tively.Target fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were prepared using genetic codon extension technology,and were purified by His Trap Chelating HP column,then biotinylated by Avitag/BirA system.Photo-conjugation efficiency,crosslink site and antigen-binding activity of photo-conjugates were investigated by SDS-PAGE,control experiment,and competitive ELISA.Results:Three expression vectors were successfully constructed,and three designed photoactivatable fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were successfully expressed with molecular weights consistent with their theoretical values.Heavy-chain conjugation efficiencies of biotinylated fusion protein with goat IgG were 85%with Fc-specific conjugation,and conjugates maintained its antigen-binding activity after photo-conjugation.Conclusion:CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 fusion proteins are successfully prepared,and mediates by those photosensitive fusion proteins,biotins molecules are conjugated to Fc fragment of goat IgG,results site-specific biotinylation of goat IgG.

Purpose To analyze the application value of enhanced T2* weighted angiography(ESWAN)quantitative parameters in predicting the invasiveness of cystic gland tumors.Materials and Methods A total of 81 patients with ovarian cystadenoma confirmed by pathological examination in Central Hospital of Xuancheng City,Anhui Province from June 2018 to June 2023 were retrospective selected.All patients underwent pelvic MRI examination(including ESWAN sequence).The ESWAN data was processed to obtain amplitude,phase,R2* and T2*values,and the quantitative intratumoral susceptibility signal(ITSS)ratio was automatically obtained using AnatomySketch software.According to the invasiveness of ovarian cystic gland tumors,they were divided into malignant cystic gland tumor group and benign cystic gland tumor group(including benign and borderline cystic gland tumors).The differences in various parameters between the two groups were compared,and the receiver operating curve was used to evaluate the predictive efficacy of each parameter on the invasiveness of ovarian cystic gland tumors.Results Among the 81 patients with ovarian cystic tumors,29 patients were in the malignant cystic tumor group,with an incidence rate of 35.80%.The CA-125,R2* values and ITSS ratio in the malignant cystic gland tumor group were higher than those in the benign cystic gland tumor group,and the differences were statistically significant(t=15.153,14.006,8.200,all P<0.05).The R2* value(OR=3.464,95%CI 1.712-7.008,P=0.001)and ITSS ratio(OR=8.376,95%CI 1.399-14.519,P=0.002)were independent risk factors for the occurrence of malignant ovarian cystadenoma(OR>1,P<0.05);the optimal critical values for R2* value,ITSS ratio and their combined prediction of malignant ovarian cystic tumors were 5.700,0.165 and 0.557,respectively.The area under the curve was 0.942,0.870 and 0.975,respectively.The sensitivity was 82.80%,82.80%and 89.70%,and the specificity was 96.20%,75.00%and 98.10%.Conclusion R2* value and ITSS ratio are independent predictive molecules for malignant cystic tumors,and their combination can enhance the predictive efficacy of invasiveness in ovarian cystic tumors.

Objective:To compare the effectiveness and safety of in vitro fenestration and in situ fenestration for reconstruction of the left subclavian artery in patients with aortic arch diseases.Methods:A retrospective analysis was conducted on 80 patients with thoracic aortic dissection, aortic aneurysm, and aortic intramural hematoma involving the left subclavian artery at our center from Jan 2020 to Oct 2023.Results:Thirty-eight patients underwent in vitro fenestration to reconstruct the left subclavian artery, while 42 patients underwent in situ laser fenestration to reconstruct the left subclavian artery. The technical success rates were 97.4% and 97.6% respectively, without statistically significance ( P>0.05). Postoperative CTA examination showed that the primary rupture of the dissection was completely closed, 3 cases had type Ⅱ endoleak in vitro fenestration, and 2 cases had type Ⅱ endoleak in laser in situ fenestration. The blood flow inside the fenestration stent was fluent. During the perioperative period, one patient in the in vitro fenestration group experienced mild cerebral infarction (2.6%), one patient had mild paraplegia, and no related complications occurred in the other patients. Conclusion:For patients with aortic arch diseases, both in situ laser fenestration and in vitro fenestration are safe and effective.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.