Objective:To observe the clinical efficacy of hyperbaric oxygen（HBO）combined with infliximab in the treatment of ulcerative colitis and explore its possible action mechanism.Methods:A total of 50 patients with ulcerative colitis were randomly divided into treatment group（26 cases）and control group（24 cases）. The treatment group was given HBO combined with infliximab，while the control group was given infliximab alone. Both groups were treated for 30 weeks.Results:The comprehensive efficacy，infliximab response rate，and trough level of the maintenance period（from the 3rd to the 5th injection）in the treatment group were significantly higher than those of the control group with statistically significant differences，respectively［92.3% vs. 66.7%， P<0.05；92.3% vs. 66.7%， P<0.01；（1.32±0.26）μg/ml vs.（0.74±0.22）μg/ml， P<0.01］；and the treatment group could significantly improve the levels of CD4 + and CD4 +/CD8 +，reduce the levels of CD8 +，TNF-α，IL-6，and CRP（ P<0.05）. Conclusion:HBO combined with infliximab in the treatment of ulcerative colitis can significantly improve the response rate of infliximab and enhance the curative effect，which may be achieved by inhibiting the proliferation of T lymphocyte and reducing the release of inflammatory factors.

Objective:To observe the clinical efficacy of hyperbaric oxygen（HBO）combined with infliximab in the treatment of ulcerative colitis and explore its possible action mechanism.Methods:A total of 50 patients with ulcerative colitis were randomly divided into treatment group（26 cases）and control group（24 cases）. The treatment group was given HBO combined with infliximab，while the control group was given infliximab alone. Both groups were treated for 30 weeks.Results:The comprehensive efficacy，infliximab response rate，and trough level of the maintenance period（from the 3rd to the 5th injection）in the treatment group were significantly higher than those of the control group with statistically significant differences，respectively［92.3% vs. 66.7%， P<0.05；92.3% vs. 66.7%， P<0.01；（1.32±0.26）μg/ml vs.（0.74±0.22）μg/ml， P<0.01］；and the treatment group could significantly improve the levels of CD4 + and CD4 +/CD8 +，reduce the levels of CD8 +，TNF-α，IL-6，and CRP（ P<0.05）. Conclusion:HBO combined with infliximab in the treatment of ulcerative colitis can significantly improve the response rate of infliximab and enhance the curative effect，which may be achieved by inhibiting the proliferation of T lymphocyte and reducing the release of inflammatory factors.

Objective:To observe and explore the clinical effect and mechanism of Tongxie Yaofang formula combined with hyperbaric oxygen (HBO) in the treatment of ulcerative colitis (UC) with liver stagnation and spleen deficiency syndrome.Methods:A total of 98 patients diagnosed as ulcerative colitis at the Department of Anorectal Medicine of the Affiliated Hospital of Qingdao University from March 2019 to September 2019 were selected as the research subjects and divided into observation group ( n=52) and control group ( n=46) according to treatment methods. The observation group was treated with Tongxie Yaofang formula combined with HBO, while the control group was treated with mesalazine sustained-release granules. The clinical efficacy and Mayo scores were evaluated after 28 days of treatment. The serum levels of tumor necrosis factor α (TNF-α) and interleukin-17 (IL-17) were detected by enzyme-linked immunosorbent assay (ELISA). The contents of prostaglandin endoperoxide synthase 2 (PTGS2) and B-cell lymphoma 2 (Bcl-2) proteins were detected by Western blotting. Fresh colon tissues before and after treatment were taken with biopsy forceps, and the contents of Yes-associated protein 1 (Yap1) and Phospho-Yes-associated protein 1 (PYap1) (Ser127) in colon tissue before and after treatment were detected by Western blotting. The healing of colonic mucosal edema and ulcer was observed by endoscope. The counts of neutrophilic granulocytes in the diffuse inflammatory infiltration of lamina propria of colonic mucosa, colonic mucosal surface ulcer, and crypt epithelium were observed microscopically before and after treatment. Results:After 28 days of treatment, the clinical effective rate in the observation group (80.8%) was significantly higher than that in the control group (60.9%) ( P<0.05). Compared with those before treatment, the Mayo scores after treatment were significantly lower in both groups ( P<0.05). The Mayo score in the observation group was lower than that in the control group ( P<0.05). After treatment, the colonic mucosal edema and ulcer were improved, and the conditions of the observation group were better than those of the control group. Pathological observations showed that the patients in the two groups, before treatment, had diffuse inflammatory infiltration of the lamina propria of the colonic mucosa, irregular glands, destruction of the crypt epithelium, abscess, and surface ulcer. After treatment, the counts of neutrophilic granulocytes in the lamina propria and the crypt epithelium were significantly reduced, and the diffuse inflammatory infiltration turned into focal infiltration. The serum levels of TNF-α and IL-17 in both groups were significantly lower than those before treatment ( P<0.05). However, there was no significant difference in serum levels of TNF-α and IL-17 between the two groups after treatment ( P>0.05). The expressions of PTGS2 and Yap1 in colon tissues of the two groups were significantly down-regulated compared with those before treatment, and the expressions of anti-apoptotic proteins Bcl-2 and PYap1 were up-regulated( P<0.05). There was no statistically significant difference in PTGS2, Yap1, Bcl-2, and P-Yap1 levels between the two groups after treatment ( P>0.05). Conclusion:Tongxie Yaofang formula combined with HBO in the treatment of UC with liver stagnation and spleen deficiency syndrome can achieve a better clinical remission rate than that of mesalazine sustained-release granule alone, and it more strengths in reducing the modified Mayo score, reducing the level of inflammatory factors, and effectively promoting mucosal ulcer healing.

Objective:To discuss the assessment value of color Doppler ultrasonography on patients with diabetic kidney disease (DKD) treated by hyperbaric oxygen (HBO) therapy.Methods:A total of 64 DKD patients confirmed in the Affiliated Hospital of Qingdao University from October 2019 to October 2020 were divided into control group ( n=32) and HBO group ( n=32) using the random number table. The control group was given comprehensive treatments (conventional hypoglycemic and antihypertensive treatment, low-fat, low-salt, and low-sugar diet, and physical exercise), and the HBO group was given HBO based on the treatment in the control group. Color Doppler ultrasound examination was performed on each patient before and after treatment, and renal hemodynamic indicators such as peak systolic velocity (PSV), end diastolic velocity (EDV), resistance index (RI), and pulsatility index (PI) were recorded. After treatment, receiver operating characteristic (ROC) curves related to therapeutic effect were made based on the indicators of RI, PI, EDV, and PSV. Results:After treatment, the 24-hour urinary albumin excretion rate (UAER), blood urea nitrogen (BUN), EDV, PSV, RI, and PI in the control group and the HBO group were all improved compared with those before treatment, and all the above indicators in HBO group had greater improvement than those in the control group, with a statistically significant differences ( P<0.05). After treatment, the total effective rate of the HBO group (96.88%) was higher than that of the control group (75.01%), with a statistically significant difference ( P<0.05). According to the calculation, RI, PI, and EDV had some value for evaluating the efficacy of HBO on DKD patients, specifically, when the area under ROC of RI was 0.866, the cutoff value was 0.76, the sensitivity was 72.73%, and the specificity was 91.38%. Conclusion:The comprehensive treatments combined with HBO have a good effect on DKD. With the non-invasive and real-time advantages, color Doppler ultrasound can be adopted to monitor RI for assessing the efficacy of DKD treatment. When the RI cutoff value reaches 0.76, it has some value for evaluating the curative effect of DKD treatment.

Objective:To observe and explore the clinical effect and mechanism of Tongxie Yaofang formula combined with hyperbaric oxygen (HBO) in the treatment of ulcerative colitis (UC) with liver stagnation and spleen deficiency syndrome.Methods:A total of 98 patients diagnosed as ulcerative colitis at the Department of Anorectal Medicine of the Affiliated Hospital of Qingdao University from March 2019 to September 2019 were selected as the research subjects and divided into observation group ( n=52) and control group ( n=46) according to treatment methods. The observation group was treated with Tongxie Yaofang formula combined with HBO, while the control group was treated with mesalazine sustained-release granules. The clinical efficacy and Mayo scores were evaluated after 28 days of treatment. The serum levels of tumor necrosis factor α (TNF-α) and interleukin-17 (IL-17) were detected by enzyme-linked immunosorbent assay (ELISA). The contents of prostaglandin endoperoxide synthase 2 (PTGS2) and B-cell lymphoma 2 (Bcl-2) proteins were detected by Western blotting. Fresh colon tissues before and after treatment were taken with biopsy forceps, and the contents of Yes-associated protein 1 (Yap1) and Phospho-Yes-associated protein 1 (PYap1) (Ser127) in colon tissue before and after treatment were detected by Western blotting. The healing of colonic mucosal edema and ulcer was observed by endoscope. The counts of neutrophilic granulocytes in the diffuse inflammatory infiltration of lamina propria of colonic mucosa, colonic mucosal surface ulcer, and crypt epithelium were observed microscopically before and after treatment. Results:After 28 days of treatment, the clinical effective rate in the observation group (80.8%) was significantly higher than that in the control group (60.9%) ( P<0.05). Compared with those before treatment, the Mayo scores after treatment were significantly lower in both groups ( P<0.05). The Mayo score in the observation group was lower than that in the control group ( P<0.05). After treatment, the colonic mucosal edema and ulcer were improved, and the conditions of the observation group were better than those of the control group. Pathological observations showed that the patients in the two groups, before treatment, had diffuse inflammatory infiltration of the lamina propria of the colonic mucosa, irregular glands, destruction of the crypt epithelium, abscess, and surface ulcer. After treatment, the counts of neutrophilic granulocytes in the lamina propria and the crypt epithelium were significantly reduced, and the diffuse inflammatory infiltration turned into focal infiltration. The serum levels of TNF-α and IL-17 in both groups were significantly lower than those before treatment ( P<0.05). However, there was no significant difference in serum levels of TNF-α and IL-17 between the two groups after treatment ( P>0.05). The expressions of PTGS2 and Yap1 in colon tissues of the two groups were significantly down-regulated compared with those before treatment, and the expressions of anti-apoptotic proteins Bcl-2 and PYap1 were up-regulated( P<0.05). There was no statistically significant difference in PTGS2, Yap1, Bcl-2, and P-Yap1 levels between the two groups after treatment ( P>0.05). Conclusion:Tongxie Yaofang formula combined with HBO in the treatment of UC with liver stagnation and spleen deficiency syndrome can achieve a better clinical remission rate than that of mesalazine sustained-release granule alone, and it more strengths in reducing the modified Mayo score, reducing the level of inflammatory factors, and effectively promoting mucosal ulcer healing.

Objective:To discuss the assessment value of color Doppler ultrasonography on patients with diabetic kidney disease (DKD) treated by hyperbaric oxygen (HBO) therapy.Methods:A total of 64 DKD patients confirmed in the Affiliated Hospital of Qingdao University from October 2019 to October 2020 were divided into control group ( n=32) and HBO group ( n=32) using the random number table. The control group was given comprehensive treatments (conventional hypoglycemic and antihypertensive treatment, low-fat, low-salt, and low-sugar diet, and physical exercise), and the HBO group was given HBO based on the treatment in the control group. Color Doppler ultrasound examination was performed on each patient before and after treatment, and renal hemodynamic indicators such as peak systolic velocity (PSV), end diastolic velocity (EDV), resistance index (RI), and pulsatility index (PI) were recorded. After treatment, receiver operating characteristic (ROC) curves related to therapeutic effect were made based on the indicators of RI, PI, EDV, and PSV. Results:After treatment, the 24-hour urinary albumin excretion rate (UAER), blood urea nitrogen (BUN), EDV, PSV, RI, and PI in the control group and the HBO group were all improved compared with those before treatment, and all the above indicators in HBO group had greater improvement than those in the control group, with a statistically significant differences ( P<0.05). After treatment, the total effective rate of the HBO group (96.88%) was higher than that of the control group (75.01%), with a statistically significant difference ( P<0.05). According to the calculation, RI, PI, and EDV had some value for evaluating the efficacy of HBO on DKD patients, specifically, when the area under ROC of RI was 0.866, the cutoff value was 0.76, the sensitivity was 72.73%, and the specificity was 91.38%. Conclusion:The comprehensive treatments combined with HBO have a good effect on DKD. With the non-invasive and real-time advantages, color Doppler ultrasound can be adopted to monitor RI for assessing the efficacy of DKD treatment. When the RI cutoff value reaches 0.76, it has some value for evaluating the curative effect of DKD treatment.