Objective To explore the effectiveness of auricular Gua Sha based on auricular vagus nerve stimulation in patients with chronic renal failure accompanied by nausea and vomiting.Methods Using a convenience sampling method,100 patients with chronic renal failure and nausea and vomiting admitted to the Nephrology Department of a tertiary hospital in Zhejiang Province between January 2022 and December 2023 were selected.They were randomly divided into a test group and a control group,with 50 patients in each group.The test group received the conventional treatment and care for chronic renal failure with nausea and vomiting,along with auricular Gua Sha,with a 3-day intervention.The control group received only conventional treatment and care.The Traditional Chinese Medicine(TCM)symptom scores for nausea and vomiting,total effective rate of the efficacy index,symptom improvement time,total number of bowel movements,and adverse events were compared between the 2 groups.Results A total of 100 patients completed the study.After intervention,the scores for nausea and vomiting symptoms were lower in both groups compared to the scores before intervention,with the experimental group having lower scores than the control group(P＜0.001);the total effective rate of the experimental group was 98.00％,higher than 88.00％in the control group(P＜0.001).Following intervention,the time for improvement of nausea and vomiting symptoms in the experimental group was(7.50±1.74)minutes,shorter than(13.38±2.31)minutes in the control group(P＜0.001);the total number of bowel movements in the experimental group was(3.34±0.63)times,more than(2.46±0.58)times in the control group(P＜0.001);no adverse events occurred during the study.Conclusion Auricular Gua Sha based on auricular vagus nerve stimulation helps reduce nausea and vomiting symptom scores,improves the total effective rate of the efficacy index,shortens the symptom improvement time,increases the frequency of bowel movements,and it is safe for patients with chronic renal failure.

Objective To explore the effectiveness of auricular Gua Sha based on auricular vagus nerve stimulation in patients with chronic renal failure accompanied by nausea and vomiting.Methods Using a convenience sampling method,100 patients with chronic renal failure and nausea and vomiting admitted to the Nephrology Department of a tertiary hospital in Zhejiang Province between January 2022 and December 2023 were selected.They were randomly divided into a test group and a control group,with 50 patients in each group.The test group received the conventional treatment and care for chronic renal failure with nausea and vomiting,along with auricular Gua Sha,with a 3-day intervention.The control group received only conventional treatment and care.The Traditional Chinese Medicine(TCM)symptom scores for nausea and vomiting,total effective rate of the efficacy index,symptom improvement time,total number of bowel movements,and adverse events were compared between the 2 groups.Results A total of 100 patients completed the study.After intervention,the scores for nausea and vomiting symptoms were lower in both groups compared to the scores before intervention,with the experimental group having lower scores than the control group(P＜0.001);the total effective rate of the experimental group was 98.00％,higher than 88.00％in the control group(P＜0.001).Following intervention,the time for improvement of nausea and vomiting symptoms in the experimental group was(7.50±1.74)minutes,shorter than(13.38±2.31)minutes in the control group(P＜0.001);the total number of bowel movements in the experimental group was(3.34±0.63)times,more than(2.46±0.58)times in the control group(P＜0.001);no adverse events occurred during the study.Conclusion Auricular Gua Sha based on auricular vagus nerve stimulation helps reduce nausea and vomiting symptom scores,improves the total effective rate of the efficacy index,shortens the symptom improvement time,increases the frequency of bowel movements,and it is safe for patients with chronic renal failure.

Objective To investigate the expression of aquaporin-8(AQP8)and apoptosis associated bcl-2 protein in human cervical carcinoma and their relationship.Methods The expression of AOP8 and bcl-2 protein in 74 cases of cervical carcinoma (46 cases of squamous-cell carcinoma of the uterine cervix,28 cases of adenocarcinoma of the uterine cervix),34 cases of cervical intraepithelial neoplasia (CIN)and 15 cases of normal cervices were detected by immunohistochemical technique,and their clinical significance were analyzed.Results The expression of AQP8 and bcl-2 protein were detected in intracytoplasm of atypia cells in CIN.squamous-cell carcinoma and adenocarcinoma of the uterine cervix.The positive rates of AQP8 and bcl-2 in squamous-cell carcinoma.adenocarcinoma,CIN and normal cervical epithelium were 98%,74%;61%,71%;71%,53%;53%,20%respectively.There were significant differences between squamous-cell carcinoma of the uterine cervix and other groups in AQP8(P＜0.01),but no significant differences were found in any other groups.There were significant differences between squamous-cell carcinoma of the uterine cervix and CIN or normal cervical epithelium in bcl-2.so were between adenocarcinoma of the uterine cervix.The expression of AQP8 was positively correlated with bcl-2 in human cervical carcinoma(rs=0.463,P=0.000).Conclusions There is a close relationship between high expression of AQP8 and development of human cervical carcinoma.The expression of AQP8 protein is positively correlated with bcl-2 protein in human cervical carcinoma. AQP8 protein may have anti-apoptosis function.although the detailed mechanism in human cervical carcinoma remaias to be clarified.

Objectives To investigate HPV infection and its correlati on with the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibito r of metalloproteinase-1 (TIMP-1) in vulvar squamous cell carcinoma (VSCC). Meth ods HPV6/11 and 16/18 DNA were detected using in situ hybridization in 26 case s of VSCC, 21 cases of vulvar intraepithelial neoplasia (VIN) and 10 specimens o f normal skin. The expression of MMP-9 and TIMP-1 was measured by immunohistoche mical method in these three groups. Results The infection rates of HPV6/11 and 16/18 in VSCC and VIN were 69.23% (18/26) and 38.46% (10/26), 42.86% (9/21) and 28.57% (6/21) respectively, and no HPV6/11 or 16/18 DNA was discovered in norma l skin epidermis. The expression rates of MMP-9 and TIMP-1 in VSCC, VIN and norm al skin epidermis were 92.31%(24/26) and 76.92% (20/26), 90.45% (19/21) and 80.9 5% (17/21), and 80.00% (8/10) and 50.00% (5/10) respectively. MMP-9 expression in HPV positive lesions was higher than that in HPV negative lesions, but no sig nificant difference of TIMP-1 expression was observed in HPV positive and negati ve lesions. Conclusions HPV infection may play a role in the development of VS CC. The higher expression of MMP-9 comparing with that of TIMP-1 may be an early marker of tumor progression in VSCC, and HPV infection may facilitate the invas ion and metastasis of VSCC.