Objective To detect the expression of sphingosine kinase 1(Sphk1)in colon cancer patients and colon cancer cells so as to explore the relationship between Sphk1 and oxaliplatin resistance in colon cancer.Methods The colon cancer RNA high-throughput sequencing data were downloaded from The Cancer Genome Atlas(TCGA),and the differences in Sphk1 expression in colon cancer tissues and normal tissues were analyzed.Human colon cancer SW48,HCT116,HT29 and LoVo cells were cultured,and the cells were randomly divided into blank control group,oxaliplatin group,transfected negative control siRNA group,transfected Sphk1 siRNA group,Sphk1 inhibitor dimethylsphingosine(DMS)group,and oxaliplatin and DMS combination group.Western blotting was used to detect the protein expressions of Sphk1 and phosphorylated Sphk1(p-Sphk1)in colon cancer cells.ELISA kits were used to detect Sphk1 activity and sphingosine-1-phosphate(S1P)content.MTT was used to detect the effect of oxaliplatin on cell viability.The nude mice planted tumor model of colon cancer cells was prepared and relevant interventions were performed.The tumor growth curve was drawn,and the expression of p-Sphk1 in the tumor was detected by Western blotting.Results The bioinformatics analysis of the colon cancer sequencing data in the TCGA database showed that the expression of Sphk1 in colon cancer tissues was significantly higher than that in normal tissues.Western blotting revealed that the expressions of Sphk1 and p-Sphk1 in HT29 and LoVo cells were higher than those in SW48 and HCT116 cells.ELISA detection found that the activity of Sphk1 in HT29 and LoVo cells was significantly higher than that in SW48 and HCT116 cells.MTT assay indicated that HT29 and LoVo cells showed drug resistance to oxaliplatin,and blocking the expression of Sphk1 with Sphk1 siRNA could restore the sensitivity of cells to oxaliplatin.Compared with the single application,the combined application of DMS and oxaliplatin could synergistically inhibit the viability of tumor cells,the activity of Sphk1 and the expression of S1P.In the nude mouse xenograft tumor models of colon cancer cells,the inhibitory effect of the combined administration of oxaliplatin and DMS on the growth of xenograft tumors was significantly stronger than the inhibitory effect of the single administration group,and it could synergistically reduce the expression of p-Sphk1 in the xenograft tumors and the concentration of S1P in the animal serum.Conclusion The expression and activation of Sphk1 are related to the sensitivity of colon cancer to oxaliplatin.Blocking Sphk1 can reverse the resistance of colon cancer cells to oxaliplatin and enhance the effects of oxaliplatin in vitro and in vivo.

Objective: To design and establish a new method for flow cytometry-based detection of commonly observed highly expressed antigens on red blood cells, and to further evaluate the differences and distribution characteristics of antigen expression levels between ABO blood type homozygotes and heterozygotes in healthy individuals. Methods: Residual blood samples after donor blood type identification by Shanghai Blood Center in April 2024 were collected. Among them, samples of 19 homozygous and 19 heterozygous individuals of type A and type B were selected. Then the expression level of ABO antigen on red blood cells were detected using the new method established in this study and the traditional aldehyde fixed red blood cell method. Both methods were tested independently three times and the results were compared. Results: The mean values of the three detection results of the new method was (×10 /RBC): AA homozygous 3.3±0.5, AO heterozygous 2.8±0.3, BB homozygous 3.6±0.3, BO heterozygous 3.1±2.8. The mean values of the three detection results of the aldehyde fixation method were AA homozygous 5.9±0.9, AO heterozygous 5.0±1.4, BB homozygous 3.8±0.6, and BO heterozygous 3.3±0.4. The average antigen distribution of each genotype followed a normal distribution. Comparing the average antigen expression levels of homozygotes and heterozygotes, both methods showed that A/B homozygotes had higher antigen levels than heterozygotes, with AA being 1.17 to 1.18 times that of AO and BB being 1.15 to 1.16 times that of BO. Comparing the inter batch differences in the three test results of two methods, the new method showed no significant difference in the three test results for four genotypes (P>0.05). The aldehyde fixation method showed significant differences in the test results for all three genotypes (P<0.01) except for BB homozygotes (P>0.05). The reliability and reproducibility of the new method were better than those of the traditional aldehyde fixation method. Conclusion: The antigen expression level of ABO homozygotes is higher than that of heterozygotes, and the difference in antigen level between type A homozygotes and heterozygotes is slightly higher than that of type B. The new method is superior to traditional aldolization fixation methods.

Objective To detect the expression of sphingosine kinase 1(Sphk1)in colon cancer patients and colon cancer cells so as to explore the relationship between Sphk1 and oxaliplatin resistance in colon cancer.Methods The colon cancer RNA high-throughput sequencing data were downloaded from The Cancer Genome Atlas(TCGA),and the differences in Sphk1 expression in colon cancer tissues and normal tissues were analyzed.Human colon cancer SW48,HCT116,HT29 and LoVo cells were cultured,and the cells were randomly divided into blank control group,oxaliplatin group,transfected negative control siRNA group,transfected Sphk1 siRNA group,Sphk1 inhibitor dimethylsphingosine(DMS)group,and oxaliplatin and DMS combination group.Western blotting was used to detect the protein expressions of Sphk1 and phosphorylated Sphk1(p-Sphk1)in colon cancer cells.ELISA kits were used to detect Sphk1 activity and sphingosine-1-phosphate(S1P)content.MTT was used to detect the effect of oxaliplatin on cell viability.The nude mice planted tumor model of colon cancer cells was prepared and relevant interventions were performed.The tumor growth curve was drawn,and the expression of p-Sphk1 in the tumor was detected by Western blotting.Results The bioinformatics analysis of the colon cancer sequencing data in the TCGA database showed that the expression of Sphk1 in colon cancer tissues was significantly higher than that in normal tissues.Western blotting revealed that the expressions of Sphk1 and p-Sphk1 in HT29 and LoVo cells were higher than those in SW48 and HCT116 cells.ELISA detection found that the activity of Sphk1 in HT29 and LoVo cells was significantly higher than that in SW48 and HCT116 cells.MTT assay indicated that HT29 and LoVo cells showed drug resistance to oxaliplatin,and blocking the expression of Sphk1 with Sphk1 siRNA could restore the sensitivity of cells to oxaliplatin.Compared with the single application,the combined application of DMS and oxaliplatin could synergistically inhibit the viability of tumor cells,the activity of Sphk1 and the expression of S1P.In the nude mouse xenograft tumor models of colon cancer cells,the inhibitory effect of the combined administration of oxaliplatin and DMS on the growth of xenograft tumors was significantly stronger than the inhibitory effect of the single administration group,and it could synergistically reduce the expression of p-Sphk1 in the xenograft tumors and the concentration of S1P in the animal serum.Conclusion The expression and activation of Sphk1 are related to the sensitivity of colon cancer to oxaliplatin.Blocking Sphk1 can reverse the resistance of colon cancer cells to oxaliplatin and enhance the effects of oxaliplatin in vitro and in vivo.

OBJECTIVE: To compare the value of video laryngoscope in localization diagnosis of upper airway stricture in obstructive sleep apnea-hypopnea syndrome (OSAHS) between awake and drug-induced sleep. METHOD: Ninety-eight patients of OSAHS were examined with video laryngoscope to locate the upper airway stricture under awake and Midazolam-induced sleep. RESULT: The several stricture levels under awake was 58.2% and it was 77.5% under drug induced sleep. CONCLUSION Several stricture levels of upper airway were founded in most of the patients of OSAHS. The upper airway stricture levels was more in the sleep than in the wake.
Adult ; Female ; Humans ; Laryngoscopes ; Male ; Middle Aged ; Sleep ; drug effects ; Sleep Apnea, Obstructive ; diagnosis ; Videotape Recording ; Wakefulness

OBJECTIVE: To investigate the value of video laryngoscope in localization diagnosis of upper airway stricture in obstructive sleep apnea-hypopnea syndrome (OSAHS) during drug-induced sleep. METHOD: One hundred and thirty two patients with OSAHS were randomly divided into group A and B in this study. In group A, the patients were examined with video laryngoscope to locate the upper airway stricture under midazolam-induced sleep. In group B, the patients had conventional physical examination and video laryngoscope when awake. And the operation plans of both groups were made based on the localization diagnosis results. RESULT: In group A, 72.1% patients have several stricture levels and in group B only 33.8%. The patients was followed up time more than 6 months, and the cure rate, the excellent effective rate, and the total effective rate of group A were significantly higher than those in group B respectively (P<0.05). CONCLUSION Most patients of OSAHS have several stricture levels of upper airway under Midazolam- induced sleep. Localization diagnosis of upper airway stricture in patients with OSAHS with video laryngoscope before operation can improve the cure efficiency significantly.
Adult ; Female ; Humans ; Laryngoscopes ; Male ; Midazolam ; Middle Aged ; Sleep Apnea, Obstructive ; diagnosis

AIM: To investigate the changes of hemodynamic parameters and ECG in circulatory failure rats induced by organophosphate insecticides DDV and parathion.METHODS: Healthy Wistar male rats,weighing(320?20)g,were treated with organophosphate insecticides by ip.to induce circulatory failure.When the mean blood pressure(MBP) decreased to 45 mmHg,the changes of hemodynamic parameters and ECG were observed.RESULTS: In circulatory failure rats,SBP,DBP,MBP,HR,LVDP,IP,+dp/dtmax,-dp/dtmax,Vpm and +dp/dtmax/IP changed dramatically(P