Purpose: Radical cystectomy (RC) is associated with substantial postoperative morbidity, and unplanned readmission remains common despite advances in perioperative management. However, the association between postoperative readmission due to complications and oncologic outcomes after RC for bladder cancer has not been clearly defined. We evaluated the impact of postoperative readmission on overall survival (OS) and cancer-specific survival (CSS) after RC for bladder cancer. Materials and Methods: We retrospectively analyzed 3,972 patients who underwent RC for bladder cancer in a multicenter cohort. Postoperative readmission was defined as unplanned hospitalization within 90 days postsurgery due to surgery-related complications. Survival outcomes were assessed using the Kaplan-Meier method and compared using the log-rank test. Univariable and multivariable Cox proportional hazards regression analyses were performed to identify independent predictors of OS and CSS. Results: Among the study population, 916 patients (23.1%) experienced postoperative readmission. Baseline and perioperative characteristics were generally comparable between patients with and without readmission. Kaplan-Meier analyses demonstrated significantly worse OS and CSS among patients who experienced postoperative readmission (both log-rank p<0.001). In multivariable analyses adjusting for clinicopathological factors, postoperative readmission remained independently associated with worse OS (hazard ratio [HR], 1.654; 95% confidence interval [CI], 1.464–1.868; p<0.001) and CSS (HR, 1.761; 95% CI, 1.509–2.055; p<0.001). Conclusion Postoperative readmission within 90 days after RC was independently associated with inferior long-term oncologic outcomes. These findings suggest the importance of strategies aimed at preventing postoperative complications and subsequent readmission.

This study aimed to establish an organoid culture model using small intestine tissues from male and female C57BL/6 mice and to compare it with rat organoid cultures derived from frozen tissues. Crypts were isolated from the small intestines of eight-week-old male and female mice and cultured in 3D extracellular matrix with Wnt, R-spondin, and Noggin. In addition, small intestine tissues from sixteen-week-old F344 rats were preserved in a storage solution immediately post-sacrifice and stored at –80°C before being transferred to a nitrogen tank. Upon thawing, crypts from frozen rat tissues failed to develop into organoids due to structural damage, suggesting the need for fresh tissues or optimized preservation methods. In contrast, mouse-derived organoids showed viability for 7 days, with distinct morphological changes and clear differentiation by Day 7. Quantitative real-time PCR analysis revealed that Lgr5, a stem cell marker, showed significantly higher expression in organoids than in tissues, confirming the successful establishment of the organoid culture. Among epithelial markers, the antimicrobial enzyme Lyz1 was more highly expressed in organoids, while Muc2, a key goblet cell marker, was more highly expressed in male tissues. The enterocyte marker Alp exhibited higher expression in male organoids compared to females, with no sex differences in tissues. These findings highlight sex-specific differences in gene expression related to small intestine differentiation and demonstrate the challenges in organoid culture from frozen rat tissues. The results suggest the importance of immediate tissue processing or improved preservation methods for successful organoid cultures.

Purpose: : This study examined the effects of a multimodal intervention (earplugs, eye patches, and aromatherapy) on sleep quality and duration in intensive care unit(ICU)patients and the feasibility of the intervention to address sleep disturbances. Methods: : This experimental study comprised a nonequivalent control group and non-synchronized design. The participants included 50 adult patients admitted to the ICU with an internal medicine diagnosis who did not receive postoperative care. The intervention was conducted on a random day from 10 pm to 5 am the following morning. Subjective sleep quality was measured using the Korean version of the Richards–Campbell Sleep Questionnaire, and objective sleep duration was recorded using a smartwatch (Galaxy Watch 5; Samsung Electronics). Data were collected from September 2023 to March 2024 and analyzed using a t-test, χ2 test, Fisher’s exact test, and analysis of covariance. Results: : The multimodal intervention significantly improved the subjective andobjective measures of sleep quality. The experimental group had higher scores on sleep depth, falling asleep, sleeping without awakening, returning to sleep, and overall sleep quality. Objective measures revealed longer total sleep time, actual sleep time, light sleep, and deep sleep in the experimental group, with no differences in rapid eye movement sleep, sleep efficiency, or ratio of awakening. Conclusion : The multimodal sleep intervention effectively improved patients’ sleep quality and duration, demonstrating the feasibility of using multimodal interventions to improve sleep quality in clinical settings.

This study aimed to establish an organoid culture model using small intestine tissues from male and female C57BL/6 mice and to compare it with rat organoid cultures derived from frozen tissues. Crypts were isolated from the small intestines of eight-week-old male and female mice and cultured in 3D extracellular matrix with Wnt, R-spondin, and Noggin. In addition, small intestine tissues from sixteen-week-old F344 rats were preserved in a storage solution immediately post-sacrifice and stored at –80°C before being transferred to a nitrogen tank. Upon thawing, crypts from frozen rat tissues failed to develop into organoids due to structural damage, suggesting the need for fresh tissues or optimized preservation methods. In contrast, mouse-derived organoids showed viability for 7 days, with distinct morphological changes and clear differentiation by Day 7. Quantitative real-time PCR analysis revealed that Lgr5, a stem cell marker, showed significantly higher expression in organoids than in tissues, confirming the successful establishment of the organoid culture. Among epithelial markers, the antimicrobial enzyme Lyz1 was more highly expressed in organoids, while Muc2, a key goblet cell marker, was more highly expressed in male tissues. The enterocyte marker Alp exhibited higher expression in male organoids compared to females, with no sex differences in tissues. These findings highlight sex-specific differences in gene expression related to small intestine differentiation and demonstrate the challenges in organoid culture from frozen rat tissues. The results suggest the importance of immediate tissue processing or improved preservation methods for successful organoid cultures.

Purpose: : This study examined the effects of a multimodal intervention (earplugs, eye patches, and aromatherapy) on sleep quality and duration in intensive care unit(ICU)patients and the feasibility of the intervention to address sleep disturbances. Methods: : This experimental study comprised a nonequivalent control group and non-synchronized design. The participants included 50 adult patients admitted to the ICU with an internal medicine diagnosis who did not receive postoperative care. The intervention was conducted on a random day from 10 pm to 5 am the following morning. Subjective sleep quality was measured using the Korean version of the Richards–Campbell Sleep Questionnaire, and objective sleep duration was recorded using a smartwatch (Galaxy Watch 5; Samsung Electronics). Data were collected from September 2023 to March 2024 and analyzed using a t-test, χ2 test, Fisher’s exact test, and analysis of covariance. Results: : The multimodal intervention significantly improved the subjective andobjective measures of sleep quality. The experimental group had higher scores on sleep depth, falling asleep, sleeping without awakening, returning to sleep, and overall sleep quality. Objective measures revealed longer total sleep time, actual sleep time, light sleep, and deep sleep in the experimental group, with no differences in rapid eye movement sleep, sleep efficiency, or ratio of awakening. Conclusion : The multimodal sleep intervention effectively improved patients’ sleep quality and duration, demonstrating the feasibility of using multimodal interventions to improve sleep quality in clinical settings.

Purpose: : This study examined the effects of a multimodal intervention (earplugs, eye patches, and aromatherapy) on sleep quality and duration in intensive care unit(ICU)patients and the feasibility of the intervention to address sleep disturbances. Methods: : This experimental study comprised a nonequivalent control group and non-synchronized design. The participants included 50 adult patients admitted to the ICU with an internal medicine diagnosis who did not receive postoperative care. The intervention was conducted on a random day from 10 pm to 5 am the following morning. Subjective sleep quality was measured using the Korean version of the Richards–Campbell Sleep Questionnaire, and objective sleep duration was recorded using a smartwatch (Galaxy Watch 5; Samsung Electronics). Data were collected from September 2023 to March 2024 and analyzed using a t-test, χ2 test, Fisher’s exact test, and analysis of covariance. Results: : The multimodal intervention significantly improved the subjective andobjective measures of sleep quality. The experimental group had higher scores on sleep depth, falling asleep, sleeping without awakening, returning to sleep, and overall sleep quality. Objective measures revealed longer total sleep time, actual sleep time, light sleep, and deep sleep in the experimental group, with no differences in rapid eye movement sleep, sleep efficiency, or ratio of awakening. Conclusion : The multimodal sleep intervention effectively improved patients’ sleep quality and duration, demonstrating the feasibility of using multimodal interventions to improve sleep quality in clinical settings.

This study aimed to establish an organoid culture model using small intestine tissues from male and female C57BL/6 mice and to compare it with rat organoid cultures derived from frozen tissues. Crypts were isolated from the small intestines of eight-week-old male and female mice and cultured in 3D extracellular matrix with Wnt, R-spondin, and Noggin. In addition, small intestine tissues from sixteen-week-old F344 rats were preserved in a storage solution immediately post-sacrifice and stored at –80°C before being transferred to a nitrogen tank. Upon thawing, crypts from frozen rat tissues failed to develop into organoids due to structural damage, suggesting the need for fresh tissues or optimized preservation methods. In contrast, mouse-derived organoids showed viability for 7 days, with distinct morphological changes and clear differentiation by Day 7. Quantitative real-time PCR analysis revealed that Lgr5, a stem cell marker, showed significantly higher expression in organoids than in tissues, confirming the successful establishment of the organoid culture. Among epithelial markers, the antimicrobial enzyme Lyz1 was more highly expressed in organoids, while Muc2, a key goblet cell marker, was more highly expressed in male tissues. The enterocyte marker Alp exhibited higher expression in male organoids compared to females, with no sex differences in tissues. These findings highlight sex-specific differences in gene expression related to small intestine differentiation and demonstrate the challenges in organoid culture from frozen rat tissues. The results suggest the importance of immediate tissue processing or improved preservation methods for successful organoid cultures.

Purpose: : This study examined the effects of a multimodal intervention (earplugs, eye patches, and aromatherapy) on sleep quality and duration in intensive care unit(ICU)patients and the feasibility of the intervention to address sleep disturbances. Methods: : This experimental study comprised a nonequivalent control group and non-synchronized design. The participants included 50 adult patients admitted to the ICU with an internal medicine diagnosis who did not receive postoperative care. The intervention was conducted on a random day from 10 pm to 5 am the following morning. Subjective sleep quality was measured using the Korean version of the Richards–Campbell Sleep Questionnaire, and objective sleep duration was recorded using a smartwatch (Galaxy Watch 5; Samsung Electronics). Data were collected from September 2023 to March 2024 and analyzed using a t-test, χ2 test, Fisher’s exact test, and analysis of covariance. Results: : The multimodal intervention significantly improved the subjective andobjective measures of sleep quality. The experimental group had higher scores on sleep depth, falling asleep, sleeping without awakening, returning to sleep, and overall sleep quality. Objective measures revealed longer total sleep time, actual sleep time, light sleep, and deep sleep in the experimental group, with no differences in rapid eye movement sleep, sleep efficiency, or ratio of awakening. Conclusion : The multimodal sleep intervention effectively improved patients’ sleep quality and duration, demonstrating the feasibility of using multimodal interventions to improve sleep quality in clinical settings.

Purpose: Although bladder cancer occurs three to 4 times more frequently in men than in women, the relative number of deaths compared to incidence is higher in women, suggesting that women have a worse prognosis than men. Emerging evidence indicates that the activity of the sex steroid hormone pathway may play a role in bladder cancer development, with demonstrations that both androgens and estrogens have biological effects on bladder cancer in vitro and in vivo. This study investigates the influence of sex and menopausal status on recurrence and cancer-specific death (CSD) in bladder cancer patients undergoing radical cystectomy (RC). Materials and Methods: This retrospective analysis included 3,913 patients from the Korean Bladder Cancer Study Group Database who underwent RC between 2010 and 2019. Patients were categorized based on gender and menopausal status (≤50 years: premenopausal; >50 years: postmenopausal). Pathological factors, neoadjuvant chemotherapy, recurrence, and CSD rates were analyzed using chi-square and Fisher exact tests. Results: Among the 3,913 patients, 400 (10.2%) were female. Premenopausal females exhibited significantly lower recurrence rates (28.6%) compared to postmenopausal females (45.7%). CSD rates were similarly reduced in premenopausal females (12.0% vs. 22.2% in postmenopausal females). No significant sex differences in recurrence or CSD were observed among premenopausal patients. Pathological T stage, nodal status, and lymphovascular invasion were significantly associated with recurrence in males, while nodal status alone was significant in females. Neoadjuvant chemotherapy was significantly more frequently administered to male patients under the age of 50, while no difference was observed in the administration of neoadjuvant chemotherapy among female patients based on menopausal status. Conclusion Hormonal changes associated with menopause significantly influence bladder cancer outcomes in women. Premenopausal hormonal environments seem protective, underscoring the need for further research into hormone-driven mechanisms in bladder cancer.

Purpose: This study aimed to evaluate the prognostic value of a trifecta, defined as negative soft tissue surgical margin (STSM), removal of ≥16 lymph nodes, and absence of major complications (Clavien-Dindo classification grade >III) within 90 days, after radical cystectomy (RC), using a large multicenter cohort. Materials and Methods: We retrospectively analyzed data from 3,972 patients with bladder cancer who underwent RC at 11 tertiary centers in South Korea between 2003 and 2024. Survival outcomes, including overall survival (OS), cancer-specific survival (CSS), and recurrence-free survival (RFS), were compared according to trifecta status using Kaplan-Meier and Cox regression analyses. Propensity score matching was performed to adjust for baseline differences. Results: A total of 2,014 eligible patients were included in the final analysis, and the trifecta was achieved in 47.8%. Kaplan-Meier analysis demonstrated significantly improved 5- and 10-year OS (66.7% vs. 62.0%; 62.9% vs. 57.2%; p=0.002), CSS (79.3% vs. 75.4%; 77.8% vs. 73.8%; p=0.008), and RFS (62.7% vs. 57.6%; 60.8% vs. 55.2%; p=0.001) in the trifecta group. In multivariable analysis, trifecta achievement was significantly associated with better OS (HR, 0.813; p=0.008), CSS (HR, 0.787; p=0.017), and RFS (HR, 0.844; p=0.036). Among individual components, negative STSM showed the strongest prognostic effect across all endpoints. Conclusions In this large multicenter study, patients who achieved the RC trifecta exhibited significantly superior survival outcomes compared with those who did not. The trifecta may serve as a practical and standardized metric for assessing surgical quality and performance in RC. Future prospective studies are warranted to validate its prognostic and quality-assurance utility.