Objective To study the validity period of sterilized medical supplies packed by non-woven fabric or disposable paper-plastic packages and placed in different battle positions and storage conditions during long-distance navigation.Methods After autoclaving,the non-woven fabrics and paper-plastic packages stored in different battle positions on ships with different batches were tested for sterility,the validity period of the two packaging methods was determined,and the data were analysed.Results On the 150th day after sterilization,bacteria began to grow on the medical supplies packed by both packages in the machine room and engine room.On the 165th day after sterilization,bacteria began to grow on the non-woven packaging items in the dental room.On the 180th day after sterilization,bacteria began to grow on the paper plastic packaging items in the clinic(reception room).On the 180th day after sterilization,the medical supplies packed by both packages in the operation room had no bacteria growth.There was no significant difference in the validity period of sterilized medical supplies between disposable paper-plastic and non-woven packages in the same battle position(P>0.05).There was no significant difference in the validity period of sterilized medical supplies between medical clinic,dental room,and operation room(P>0.05),but there were significant differences between machine room,engine room,and operation room(P<0.05).Conclusion The term of validity of medical supplies packaged with disposable paper-plastic and non-woven fabric packages sterilized by autoclaving is both within 135 days,and there is no significant difference in the validity period date between the two packaging methods.

HMGR and DXR are key enzymes of terpenoids biosynthesis pathway. This study was aimed to discuss the effects of overexpression of HMGR and DXR from A momum villosum Lour. on the biosynthesis of terpenoids in transgenic tobacco. The real-time fluorescence quantitative PCR (RT-qPCR) was used to analyze the expression level of AvHMGR and AvDXR. Then, enzyme activities of HMGR and DXR were determined by spectrometer using the substrate-specific method. Different terpenoids were detected by GC-MS. The results showed that individual overex-pression of HMGR/DXR can inhibit the enzyme activities of HMGR and DXR but promote the biosynthesis of men-thene, neophytediene, cembrenene and sterol. The co-overexpression of HMGR and DXR had different enzyme activ-ities and can promote the biosynthesis of sterol and phytol, but inhibit the biosynthesis of neophytadiene. It was con-cluded that the overexpression of HMGR and DXR had diverse effects when regulating the biosynthesis of different terpenoids. This study provided the basis for using A vHMGR and A vDXR to regulate the metabolism of terpenoids.