Objective To analyze the clinical value of susceptibility weighted imaging(SWI)and arterial spin labeling imaging(ASL)in the prediction of hemorrhage transformation after thrombolysis in acute ischemic stroke.Methods A total of 80 patients with acute ischemic stroke admitted to Guangdong Tongjiang Hospital from December 2021 to November 2023,before and within 24 h after thrombolytic therapy,were enrolled,and all of them underwent computed tomography(CT),magnetic resonance imaging(MRI),SWI and ASL sequential scanning.The brush sign and asymmetrical cortical venous sign of patients were analyzed,and the diagnostic value of SWI and ASL for cerebral hemorrhage transformation after thrombolysis was compared.Eleven patients with cerebral hemorrhage transformation after thrombolysis were included in hemorrhagic transformation group,while the other 69 patients without hemorrhagic transformation were included in non-hemorrhagic transformation group.Logistic regression analysis was conducted to explore the influencing factors of hemorrhagic transformation after thrombolysis.Results The positive rates of brush sign and asymmetric cortical vein sign were 71.25%(57/80)and 68.75%(55/80)by SWI scanning before thrombolysis in 80 patients with acute ischemic stroke,while those were 16.25%(13/80)and 15.00%(12/80)after thrombolysis.The differences between brush sign and asymmetric cortical venous sign detected by SWI before and after thrombolysis were statistically significant(P<0.05).SWIversus ASL had sensitivity of 90.91%(10/11)versus72.73%(8/11),specificity of 95.65%(66/69)versus88.41%(61/69),and accuracy of 95.00%(76/80)versus86.25%(69/80)in the diagnosis of cerebral hemorrhage transformation after thrombolysis,revealing that SWI had higher sensitivity and specificity than ASL.The univariate analysis of cerebral hemorrhage transformation showed that the age,atrial fibrillation,NIHSS score and CMBs grade in two groups differed significantly(P<0.05);and the multivariate analysis identified atrial fibrillation,NISHH score and CMBs grade as independent risk factors of cerebral hemorrhagic transformation after thrombolysis.Conclusion SWI has high sensitivity in detecting brush sign and asymmetric cortical venous sign,which is of great value for predicting cerebral hemorrhage transformation after acute thrombolysis,and can be used as a routine examination sequence for acute ischemic stroke.

BACKGROUND:Recent studies have shown that research on naringin anti-osteoporosis mostly stays in in vitro and in vivo experiments.Understanding the mechanism of related signaling pathways and the expression of related proteins and some specific genes is an important way to deeply understand naringin anti-osteoporosis.At present,traditional Chinese medicine has been confirmed to have a significant role in anti-osteoporosis.Naringin is one of the main active ingredients in Rhizoma Drynariae.Its effectiveness and mechanism of action against osteoporosis have been gradually recognized by scholars,and its clinical and basic research has been gradually emphasized. OBJECTIVE:To analyze and summarize the research progress of naringin in anti-osteoporosis in vitro and in vivo,thereby providing some ideas for the next step to study its related mechanism of action. METHODS:The relevant literatures included in CNKI and PubMed database were searched with the Chinese search terms of"naringin,osteoporosis,traditional Chinese medicine compound,pathogenesis,signaling pathway,bone marrow mesenchymal stem cells,osteoblasts,osteoclasts"in Chinese and English,respectively.The corresponding criteria were established according to the research needs,and finally 69 articles were included for review. RESULTS AND CONCLUSION:Naringin blocks the increase in the number of osteoclasts and adipocytes,the decrease in the number of osteocytes and osteocalcin(+)cells induced by fructose-rich diet,and promotes the secretion of Sema3A from osteoblasts and osteocytes,thereby enhancing local bone formation and inhibiting osteoclast production by activating the Wnt/β-catenin pathway.Naringin is an important way to induce autophagy of osteoblasts,but autophagy-related proteins participate in osteoblast differentiation and bone formation.Lack of autophagy in osteoblasts reduces mineralization and leads to an imbalance in the number of osteoblasts and osteoclasts,which results in bone loss and decreased bone density.The composite scaffold loaded with naringin can be used as a necessary carrier for bone defect repair and has excellent bone repair properties.Naringin can also accelerate the growth of new bone tissue by increasing the local contents of bone morphogenetic protein 2 and vascular endothelial growth factor.Naringin can regulate bone metabolism and inhibit oxidative stress via ERK,PI3K/Akt and Wnt signaling pathways to improve osteoporosis,which can play a good role in preventing and controlling the disease.However,the depth and breadth of the relevant research is insufficient.Based on the mechanism of the current study,we should investigate the specific mechanisms by which naringin regulates different pathways and inter-pathway interactions in the future,which will be beneficial to the multifaceted development of naringin used in the treatment of osteoporosis..

Plant-derived nanovesicles have gained attention given their similarity to mammalian exosomes and advantages such as low cost,sustainability,and tissue targeting.Thus,they hold promise for disease treatment and drug delivery.In this study,we proposed a time-efficient method,PEG 8000 combined with sucrose density gradient centrifugation to prepare ginger-derived nanovesicles(GDNVs).Subse-quently,curcumin(CUR)was loaded onto GDNV by ultrasonic incubation.The optimum conditions for ginger-derived nanovesicles loaded with curcumin(CG)were ultrasound time of 3 min,a carrier-to-drug ratio(GDNV:CUR)of 1∶1.The study achieved a high loading capacity(94.027％±0.094％)and encapsu-lation efficiency(89.300％±0.344％).Finally,the drugs'in vivo distribution and anti-colitis activity were investigated in mice.CG was primarily distributed in the colon after oral administration.Compared to CUR and GDNV,CG was superior in improving disease activity,colon length,liver and spleen coefficients,myeloperoxidase activity,and biochemical factor levels in ulcerative colitis(UC)mice.In addition,CG plays a protective role against UC by modulating serum metabolite levels and gut flora.In summary,our study demonstrated that GDNV can be used for CUR delivery with enhanced therapeutic potential.

Plant-derived nanovesicles have gained attention given their similarity to mammalian exosomes and advantages such as low cost, sustainability, and tissue targeting. Thus, they hold promise for disease treatment and drug delivery. In this study, we proposed a time-efficient method, PEG 8000 combined with sucrose density gradient centrifugation to prepare ginger-derived nanovesicles (GDNVs). Subsequently, curcumin (CUR) was loaded onto GDNV by ultrasonic incubation. The optimum conditions for ginger-derived nanovesicles loaded with curcumin (CG) were ultrasound time of 3 min, a carrier-to-drug ratio (GDNV:CUR) of 1:1. The study achieved a high loading capacity (94.027% ± 0.094%) and encapsulation efficiency (89.300% ± 0.344%). Finally, the drugs' in vivo distribution and anti-colitis activity were investigated in mice. CG was primarily distributed in the colon after oral administration. Compared to CUR and GDNV, CG was superior in improving disease activity, colon length, liver and spleen coefficients, myeloperoxidase activity, and biochemical factor levels in ulcerative colitis (UC) mice. In addition, CG plays a protective role against UC by modulating serum metabolite levels and gut flora. In summary, our study demonstrated that GDNV can be used for CUR delivery with enhanced therapeutic potential.

Objective To analyze the clinical value of susceptibility weighted imaging(SWI)and arterial spin labeling imaging(ASL)in the prediction of hemorrhage transformation after thrombolysis in acute ischemic stroke.Methods A total of 80 patients with acute ischemic stroke admitted to Guangdong Tongjiang Hospital from December 2021 to November 2023,before and within 24 h after thrombolytic therapy,were enrolled,and all of them underwent computed tomography(CT),magnetic resonance imaging(MRI),SWI and ASL sequential scanning.The brush sign and asymmetrical cortical venous sign of patients were analyzed,and the diagnostic value of SWI and ASL for cerebral hemorrhage transformation after thrombolysis was compared.Eleven patients with cerebral hemorrhage transformation after thrombolysis were included in hemorrhagic transformation group,while the other 69 patients without hemorrhagic transformation were included in non-hemorrhagic transformation group.Logistic regression analysis was conducted to explore the influencing factors of hemorrhagic transformation after thrombolysis.Results The positive rates of brush sign and asymmetric cortical vein sign were 71.25%(57/80)and 68.75%(55/80)by SWI scanning before thrombolysis in 80 patients with acute ischemic stroke,while those were 16.25%(13/80)and 15.00%(12/80)after thrombolysis.The differences between brush sign and asymmetric cortical venous sign detected by SWI before and after thrombolysis were statistically significant(P<0.05).SWIversus ASL had sensitivity of 90.91%(10/11)versus72.73%(8/11),specificity of 95.65%(66/69)versus88.41%(61/69),and accuracy of 95.00%(76/80)versus86.25%(69/80)in the diagnosis of cerebral hemorrhage transformation after thrombolysis,revealing that SWI had higher sensitivity and specificity than ASL.The univariate analysis of cerebral hemorrhage transformation showed that the age,atrial fibrillation,NIHSS score and CMBs grade in two groups differed significantly(P<0.05);and the multivariate analysis identified atrial fibrillation,NISHH score and CMBs grade as independent risk factors of cerebral hemorrhagic transformation after thrombolysis.Conclusion SWI has high sensitivity in detecting brush sign and asymmetric cortical venous sign,which is of great value for predicting cerebral hemorrhage transformation after acute thrombolysis,and can be used as a routine examination sequence for acute ischemic stroke.

BACKGROUND:Vertebral compression fractures are the most common vertebral fractures in the elderly patients with osteoporosis, and the correlation between the compression of anterior border of vertebral body and adjacent vertebral refractures is not clear.
OBJECTIVE:To establish a model of different compression of T12vertebral body with finite element method, and analyze the relationship between the compression of T12vertebral body and the stress of adjacent vertebral plate.
METHODS:Based on thoracolumbar CT data of normal adult volunteers, MIMICS/3-matic was imported. Through image segmentation, repair and three-dimensional mesh of accessto thoracic and lumbar T11-L1data, grid assigned material properties was imported to ABAQUS so as to establish ligament, joint and other small features and obtain realistic three-dimensional finite element model. The six degrees of freedom, including anteflexion, posterior extension, left and right flexion, left and right rotation, were loaded, to verify the validity of the normal model. With the frontier of vertebral body compression to 90%, 80%, 70%…10% of the nine states, MISES stressesof the T11andL1segment intervertebral disc endplate were extracted; the relationship curve of compression state and endplate stress was obtained.
RESULTS AND CONCLUSION:(1) The model was highly realistic and could reflect the actual stress state. (2) The stress value of T11vertebral body and L1vertebral body was positively correlated with the compression of T12vertebral body. Increased stress may lead to an increased likelihood of end plate fractures, which increases the risk of fractures in the adjacent vertebralbodies.

This study examined the association of pregnancy with urolithiasis and provided new insights into urolithiasis in pregnancy. A total of 462 subjects were studied from January 2004 to December 2009 in Foshan Maternal and Child Health Hospital, China. Among the 462 subjects, 162 cases of urolithiasis during pregnancy (UPG) were selected as the observation group, 150 cases of no urolithiasis during pregnancy (NUPG) served as pregnancy control group, and 150 cases of no pregnancy (NPG) at reproductive age who took part in physical examination were randomly assigned into non-pregnant control group. At the same time, the patients in observation group were divided into the following sub-groups: no symptomatic urinary calculus (NSUC) and symptomatic urinary calculus (SUC) groups; SUC group was further divided into surgical intervention (SI) and conservative management (CM) groups. The general information and the data of blood and urine were collected and compared among the groups. The results showed that the incidence of urinary calculi in pregnant women was lower than that in non-pregnant women, the formation of urinary stone was associated with the change of metabolism of protein and sugar in pregnant women, and the surgical intervention was a practicable alternative to treat the clinical intractable symptomatic urinary calculi in pregnancy.

This study examined the association of pregnancy with urolithiasis and provided new insights into urolithiasis in pregnancy.A total of 462 subjects were studied from January 2004 to December 2009 in Foshan Maternal and Child Health Hospital,China.Among the 462 subjects,162 cases of urolithiasis during pregnancy (UPG) were selected as the observation group,150 cases of no urolithiasis during pregnancy (NUPG) served as pregnancy control group,and 150 cases of no pregnancy (NPG) at reproductive age who took part in physical examination were randomly assigned into non-pregnant control group.At the same time,the patients in observation group were divided into the following sub-groups:no symptomatic urinary calculus (NSUC) and symptomatic urinary calculus (SUC) groups;SUC group was further divided into surgical intervention (SI) and conservative management (CM) groups.The general information and the data of blood and urine were collected and compared among the groups.The results showed that the incidence of urinary calculi in pregnant women was lower than that in non-pregnant women,the formation of urinary stone was associated with the change of metabolism of protein and sugar in pregnant women,and the surgical intervention was a practicable alternative to treat the clinical intractable symptomatic urinary calculi in pregnancy.

Objective To study the diagnostic value of contrast-enhanced CT patterns in focal hepatic lesions.Methods Contrast-enhanced CT patterns in 44 patients with focal hepatic lesions (54 foci) were retrospectively analysed.Results (1)78% of the hepatocellular carcinoma presented "rapid-filling and rapid-washout" feature.(2)All hepatic hemangioma presented "rapid-filling and slow-washout", progressive opacification from the periphery to center. (3)All cholangiocarcinoma presented "slow-filling and slow-washout" .(4)Metastatic hepatocarcinoma in 14 cases and hepatic abscesses in 5 cases were presented a enhanced ring around the lesion, metastatic hepatocarcinomas showed single ring,however, hepatic abscesses showed multi-ring. (5)Focal nodular hyperplasia in 3 cases and hepatic adenomas in 1 case presented "rapid-filling and slow-washout".Conclusion The contrast-enhanced patterns at spiral CT can fully reflect the blood supply of focal hepatic lesions, it has significant value in diagnosing focal hepatic lesions.

Objective To investigate the atypical appearances of small hepatocellular carcinoma(SHCC) with triple-phase spiral CT enhanced scan, and its correlation with the histopathology .Methods The atypical CT signs in triple-phase and histopathologic changes of SHCC confirmed pathologically in 30 cases (32 lesions) were analysed.Results 32 atypical lesions were found in 30 patients,of them,14 lesions were hypodense in hepatic arterial phase(HAP),portal venous phase(PVP) and delayed phase(DP).10 lesions enhanced markedly in the AP,while these lesions became isodense or slight hyperdense in the PVP and DP.8 lesions were enhanced as ring like or punctual shape in the AP,and constant enhancement in PVP and DP.Conclusion The atypical appearances are present in triple- phase spiral CT scan in SHCC,the pattern of blood supply and the base of histopathology are usually the cause of these findings .