Objective:To study the relationship between intraoperative portal venous pressure gradient (PVPG) changes and clinical outcomes of meso-Rex bypass in pediatric patients with extrahepatic portal vein obstruction (EHPVO).Methods:Clinical data of 76 children with EHPVO undergoing Rex shunt at Guangzhou Women and Children’s Medical Center, Affiliated to Guangzhou Medical University from April 2018 to October 2021 were retrospectively analyzed, including 49 males and 27 females, aged 62.0 (42.0, 102.5) months. Logistic regression was used to identify prognostic factors. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficacy of PVPG variations. The association between pre-/postoperative PVPG differences and clinical outcomes (resolution of hypersplenism/splenomegaly, improvement of gastroesophageal varices, and vascular complications) were systematically assessed.Results:All 76 pediatric patients underwent the Rex shunt successfully. Postoperative vascular complications occurred in 14 cases (18.4%), including six cases of bypass graft thrombosis (7.9%) and eight cases of anastomotic stenosis (10.5%). Logistic regression analyses revealed that graft type ( OR=0.03, 95% CI: 0-0.30, P=0.003) and PVPG gradient ( OR=1.65, 95% CI: 1.07-2.53, P=0.022) were associated with esophagogastric varices resolution after Rex shunt. Graft type also correlated with splenomegaly/hypersplenism improvement ( OR=0.08, 95% CI: 0.01-0.80, P=0.032). PVPG gradient showed association with anastomotic stenosis ( OR=0.69, 95% CI: 0.51-0.93, P=0.014). The area under ROC curves of PVPG gradient are 0.786 and 0.815 for predicting varices resolution and anastomotic stenosis, respectively. Conclusion:An increased intraoperative PVPG gradient could serve as a protective factor for an improved outcome of Rex shunt in EHPVO children and reduced risk of anastomotic stenosis.

Objective:To study the relationship between intraoperative portal venous pressure gradient (PVPG) changes and clinical outcomes of meso-Rex bypass in pediatric patients with extrahepatic portal vein obstruction (EHPVO).Methods:Clinical data of 76 children with EHPVO undergoing Rex shunt at Guangzhou Women and Children’s Medical Center, Affiliated to Guangzhou Medical University from April 2018 to October 2021 were retrospectively analyzed, including 49 males and 27 females, aged 62.0 (42.0, 102.5) months. Logistic regression was used to identify prognostic factors. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficacy of PVPG variations. The association between pre-/postoperative PVPG differences and clinical outcomes (resolution of hypersplenism/splenomegaly, improvement of gastroesophageal varices, and vascular complications) were systematically assessed.Results:All 76 pediatric patients underwent the Rex shunt successfully. Postoperative vascular complications occurred in 14 cases (18.4%), including six cases of bypass graft thrombosis (7.9%) and eight cases of anastomotic stenosis (10.5%). Logistic regression analyses revealed that graft type ( OR=0.03, 95% CI: 0-0.30, P=0.003) and PVPG gradient ( OR=1.65, 95% CI: 1.07-2.53, P=0.022) were associated with esophagogastric varices resolution after Rex shunt. Graft type also correlated with splenomegaly/hypersplenism improvement ( OR=0.08, 95% CI: 0.01-0.80, P=0.032). PVPG gradient showed association with anastomotic stenosis ( OR=0.69, 95% CI: 0.51-0.93, P=0.014). The area under ROC curves of PVPG gradient are 0.786 and 0.815 for predicting varices resolution and anastomotic stenosis, respectively. Conclusion:An increased intraoperative PVPG gradient could serve as a protective factor for an improved outcome of Rex shunt in EHPVO children and reduced risk of anastomotic stenosis.

Objective? To explore the effects of Omaha system-based nursing intervention on patients after percutaneous coronary intervention (PCI). Methods? Totally 200 patients receiving PCI in Ningbo First Hospital from February 2016 to November 2017 were selected using convenient sampling and divided into the control group (n=100) and the observation group (n=100) according to the random number table. Patients in the control group received conventional nursing care, while patients in the observation group received nursing care based on Omaha system. The incidence of postoperative vascular complications was evaluated between the two groups. Self-Depression Scale (SDS), Self-Anxiety Scale (SAS) and the nursing satisfaction questionnaire were used to evaluate the effects of intervention. Results? The incidence rate of postoperative vascular complications was lower in the observation group than in the control group (P＜ 0.05); the SAS and SDS scores of the observation group were lower than those of the control group (P＜0.05); and the satisfaction at discharge of the observation group was higher than that of the control group (P＜ 0.05). Conclusions? Omaha system-based nursing interventions can reduce the incidence rate of postoperative vascular complications, ameliorate patients' negative emotions and improve their satisfaction, which is worth promoting in clinical practice.

Objective? To explore the effects of symposium involving patients and their family members on health education in patients with acute myocardial infarction after percutaneous transluminal coronary intervention (PCI). Methods? Totally 86 patients with acute myocardial infarction receiving PCI in Ningbo First Hospital from January 2017 to June 2018 were selected continuously and divided into the control group (n=43) and the observation group (n=43). Patients in the control group received conventional health education which combined oral education and education manuals, while patients in the observation group symposium-based health education which involved both patients and their family members on the basis of conventional education after PCI. Both groups received health education for 4 weeks. Health knowledge about acute myocardial infarction, rehabilitation initiative, postoperative complication and satisfaction with overall nursing were compared between the two groups before and after intervention. Results? The health-related knowledge of the patients with acute myocardial infarction in the observation group 4 weeks after intervention scored (94.3±4.0), higher than that of the control group, which was (78.4±5.1), and there was statistically significant difference (t=8.285, P＜ 0.05). The scores of five dimensions of rehabilitation initiative (coordination with therapy, compliance, needs for rehabilitation, emotions post frustration and encouragement from family members and nurses) of the observation group were higher than those of the control group (t=4.726, 5.114, 3.975, 7.116, 5.893; P＜0.05). The incidence rate of postoperative complication in the observation group was 9.30%, lower than that of the control group, which was 30.23%, and there was statistically significant difference (χ2=5.939, P＜0.05). The nursing satisfaction of the observation group was 96.73%, higher than that of the control group, which was 83.78%, and there was statistically significant difference (χ2=4.904, P＜0.05). Conclusions? The symposium involving patients and their family members for patients with acute myocardial infarction after PCI can improve the patients' disease-related knowledge, satisfaction with overall nursing and rehabilitation initiative, and reduce the postoperative complications effectively.

Cancer is one of the major “killers” threatening human health, but so far there is not very effective treatment. Although chemotherapy is the most commonly used for the non-surgical treatment of cancer, due to the extensive presence of tumor heterogeneity, many cases of multidrug resistance and the failure in treatment of tumors can be found everywhere. In order to avoid the blindness of chemotherapy and improve the efficiency of chemotherapy, the personalized therapy of cancer based on chemosensitivity test has come into being. After more than half a century of evolution, the new methods of chemosensitivity test have been developed continuously, with a simple, rapid, accurate and reliable trend. A series of clinical studies have confirmed the importance of chemosensitivity test in guiding personalized therapy of cancer. However, the previous literatures have not distinguished the methods of culture and detection involved in chemosensitivity test, which is easy to cause conceptual confusion. Hence, the most commonly used methods of chemosensitivity test are reorganized in this article, with emphases on the introduction of three-dimensional cell culture, bioluminescence assay, fluorescence analysis and electric cell-substrate impedance sensing, to have a more comprehensive and in-depth understanding of chemosensitivity test.