In recent years, the long-read sequencing technology has been developing rapidly, playing a crucial role in research fields of genomics, transcriptomics, and epigenetics, and showing broad application prospects in the field of clinical testing. This paper introduces the basic principles and performance of the main long-read sequencing platforms, reviews the research progress of this technology in preimplantation genetic testing, and discusses the major challenges currently faced and possible future directions.

In recent years, the long-read sequencing technology has been developing rapidly, playing a crucial role in research fields of genomics, transcriptomics, and epigenetics, and showing broad application prospects in the field of clinical testing. This paper introduces the basic principles and performance of the main long-read sequencing platforms, reviews the research progress of this technology in preimplantation genetic testing, and discusses the major challenges currently faced and possible future directions.

Objective To observe effect of muscle basal lamina containing neural stem cells (NSCs) in repair of spinal cord injury.Methods Thirty-six SD rats from the same nest were used in the study and spinal cord hemisection models were induced.The animals were classified to blank control group (clearance of the lesion edge only with isotonic saline),NSCs group (transplantation of NSCs to the edge),NSCs + muscle basal lamina group (transplantation of complex of NSCs and muscle basal lamina to the edge) according to random number table,with 12 rats per group.At weeks 4 and 8,survival and migration of the transplanted cells and compatibility of muscle basal lamina with the host were detected.At weeks 2,4,and 8,the hindlimb function was assayed using BBB scoring system.Results NSCs in NSCs + muscle basal lamina group grew at the lesion edge,migrated to both sides of the edge,and integrated with peripheral tissues.Whereas,few NSCs survived at the lesion edge in NSCs group and inflammatory cell infiltration was notable.At week 2,there was no statistical difference of BBB score among the three groups.At weeks 4 and 8,BBB score in NSCs + muscle basal lamina group (7.92 ± 1.00,11.38 ± 1.51) was significantly higher than that in blank control group (3.82 ± 0.75,3.71 ± 0.76) and NSCs group (6.25 ±1.06,8.25 ± 1.83) (P＜0.05).Conclusion Muscle basal lamina orients growth of NSCs along its lumen,facilitates migration of host cells to ground substance within its lumen,and reduces local inflammatory reaction.