Objective:To study the effect of medical social workers combined with ICU diary on the prevention and treatment of post-traumatic stress disorder in ICU traffic accident patients, and to explore the application of continuing nursing services and ICU diary to ICU traffic accident patients.Methods:A total of 47 patients who were admitted to the ICU of Suzhou Science and Technology City Hospital from January to December 2019 due to traffic accidents were selected by convenient sampling method. Twenty-three patients admitted to the hospital in the first 6 months were selected as the control group (before the introduction of medical social workers and ICU diaries) for routine ICU treatment and nursing mode. Twenty-four patients admitted to the hospital in the last 6 months were selected as the experimental group (after the introduction of medical social workers and ICU diaries), and the medical social workers combined with the ICU diary were intervened. The scores of the Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were compared between the two groups of patients when they were admitted to the ICU, stayed in the ICU for a week, and transferred out of the ICU. The incidence of post-traumatic stress disorder was evaluated and compared by the scores of the Impact of Event Scale-Revised of the two groups of patients one month after leaving the ICU, three months after leaving the ICU, and six months after leaving the ICU.Results:The scores of SAS in the experimental group were 58.67 ± 7.45, 44.13 ± 5.47 and 39.08 ± 5.52 when they were admitted to the ICU, stayed in the ICU for a week, and transferred out of the ICU, and were 58.17 ± 6.75, 50.87 ± 6.35 and 42.74 ± 4.85, respectively in the control group. There was no statistical difference in the scores of the SAS between the two groups of patients when they were admitted to the ICU ( P>0.05), but there was a time difference between the two groups with time changes and different interventions ( Ftime = 110.98, P<0.01), there were also differences between groups ( Fbetween groups = 5.91, P<0.05). Compared with the control group, the SAS score of the experimental group decreased more significantly( Finteraction = 0.28, P<0.05). The scores of SDS in the experimental group were 57.75 ± 4.06, 45.29 ± 3.39 and 36.63 ± 3.49 when they were admitted to the ICU, stayed in the ICU for a week, and transferred out of the ICU, and were 57.48 ± 2.29, 49.04 ± 5.65 and 43.57 ± 4.07 respectively in the control group. There was no statistical difference in the scores of the SDS between the two groups of patients when they were admitted to the ICU ( P>0.05), but there was a time difference between the two groups of patients with time changes and different interventions ( Ftime = 248.24, P<0.01), there are also differences between groups ( Fbetween groups = 24.39, P<0.05). Compared with the control group, the SDS score of the experimental group decreased more significantly ( Finteraction=10.44, P<0.05). The incidences of post-traumatic stress disorder in the experimental group were 9, 8, 6 cases at one month, three months and six months after leaving the ICU, which were lower than 12, 11, 8 cases in the control group. The generalized estimation equation showed that the difference was statistically significant ( χ2=4.21, P<0.05). Conclusions:The intervention model of medical social workers combined with ICU diary is conducive to the use of ICU diary and the development of continuous nursing, which improves patients′negative emotions and reduces the incidence of post-traumatic stress disorder.

Photoacoustic imaging (PAI) is a rapidly developing hybrid biomedical imaging technology, which is capable of providing structural and functional information of biological tissues. Due to inevitable motion of the imaging object, such as respiration, heartbeat or eye rotation, motion artifacts are observed in the reconstructed images, which reduce the imaging resolution and increase the difficulty of obtaining high-quality images. This paper summarizes current methods for correcting and compensating motion artifacts in photoacoustic microscopy (PAM) and photoacoustic tomography (PAT), discusses their advantages and limits and forecasts possible future work.
Artifacts ; Microscopy ; Motion ; Photoacoustic Techniques ; Tomography, X-Ray Computed

Objective:To explore the effect of moxibustion pretreatment on bone marrow suppression in cancer patients after chemotherapy, so as to provide a basis for clinical nursing.Methods:From May 2019 to May 2020, we selected 70 cancer patients who met the criteria in the Oncology Department of a ClassⅢ Grade A hospital in Xuzhou City, Jiangsu Province by convenient sampling. There were 65 patients who finally completed the trial including moxibustion pretreatment group ( n=20) , moxibustion group ( n=22) , and control group ( n=23) . All three groups received regular chemotherapy. Patients in moxibustion pretreatment group received moxibustion 7 days before the start of chemotherapy, once a day, for 14 days; patients in moxibustion group were treated with moxibustion at the beginning of chemotherapy, once a day, for 7 days; control group carried out conventional chemotherapy. We compared the white blood cell count, neutrophil count, incidence and degree of bone marrow suppression, and the use of recombinant human granulocyte colony stimulating factor among the three groups. Results:The white blood cell and neutrophil counts of the three groups were compared between the groups and at different times, and the differences were statistically significant ( P<0.05) . The incidences of bone marrow suppression on the seventh and fourteenth day after chemotherapy of the three groups of patients were compared between the groups and at different times with statistical differences ( Wald χ2groups=6.628, P=0.036; Wald χ2times=43.018, P<0.001) . A total of 7, 14 days after chemotherapy, there were statistical differences in the use of recombinant human granulocyte colony stimulating factor in patients among the three groups ( P<0.05) . Conclusions:Moxibustion pretreatment can stabilize the white blood cell and neutrophil counts of cancer patients after chemotherapy, reduce the incidence of bone marrow suppression, the degree of bone marrow suppression as well as the use of recombinant human granulocyte colony stimulating factor.

Objective:To develop the Fear of Disease Progression Scale for Patients with Acute Myocardial Infarction, and test its reliability and validity.Methods:The first draft of the scale was formed through literature review, qualitative interviews, and Delphi expert consultations. From March to July 2020, convenience sampling method was adopted to conduct a formal survey of 200 patients with acute myocardial infarction admitted to the Department of Cardiology in three ClassⅢ Grade A hospitals in Jiangsu Province. The formal scale was formed through the project analysis and exploratory factor analysis, and its reliability and validity were determined. 200 questionnaires were distributed, and 178 valid questionnaires were recovered, with a valid recovery rate of 89.00%.Results:The final scale contained 17 items, and exploratory factor analysis extracted 3 common factors, and the cumulative contribution rate of variance was 62.671%. The item-level content validity index ( I- CVI) was from 0.83 to 1.00, the scale-level content validity index ( S- CVI) was 0.82, and the average S- CVI was 0.97. The Cronbach's α coefficient of the total scale was 0.892, the half-reliability was 0.764, and the test-retest reliability was 0.964. Conclusions:The developed Fear of Disease Progression Scale for Patients with Acute Myocardial Infarction has good reliability and validity, which can be used to assess the current status of fear of disease progression in patients with acute myocardial infarction.

Objective:To explore the status quo of meaning of life in patients undergoing radical mastectomy and analyze the influencing factors.Methods:The convenient sampling method was used to select 302 patients with breast cancer who were treated in 2 Class Ⅲ Grade A hospitals in Zhenjiang from June 2019 to February 2020 as research objects. The general data questionnaire, Chinese version of Meaning in Life Questionnaire (C-MLQ) , Health-related Hardiness Scale (HRHS) and Cognitive Reassessment Strategy Scale were used to investigate them.Results:A total of 315 questionnaires were issued and 302 valid questionnaires were collected.The total score of C-MLQ of 302 patients undergoing radical mastectomy was (31.65±6.71) , the total score of HRHS was (92.59±5.68) and the total score of Cognitive Reassessment Strategy Scale was (16.23±5.04) .The results of multiple linear regression analysis showed that cognitive reassessment, postoperative time, health-related hardiness and its control dimension, family monthly income, gender, radiotherapy and chemotherapy were the influencing factors of meaning of life in patients undergoing radical mastectomy ( P<0.05) , which explained 85.8% of the variation in meaning of life. Conclusions:The meaning of life of patients undergoing radical mastectomy is at a medium low level. Clinical medical staff should take effective intervention measures to improve cognitive reassessment ability of patients, so that they can have correct cognition of the disease. They should also encourage them to face the disease actively and bravely and strengthen their own health-related hardinessin order to improve the meaning of life.

Objective To establish a quick and accurate method for detection of tree shrew adenovirus(TAV) using TaqMan real-time fluorescence quantitative PCR. Methods Based on the published TAV genome sequence, a 3' conserved sequence was used to design specific probe primers. A standard curve was prepared using a recombinant plasmid containing the target gene fragment. A real-time fluorescence quantitative PCR method was established for detecting TAV based on TaqMan probe. Results The detection method was specific and was not cross-reactive with other common pathogens. The detection limit of the method was 3.7 copies/μL,showing a high sensitivity. The correlation coefficient was 0.998, and the efficiency was 95.7%. The amplification result showed a fine linear relationship,and the repeatability test effect was good. Conclusions The TAV real-time quantitative PCR detection method based on TaqMan probe has been successfully established. It has high sensitivity and reproducibility and can be applied to early detection of TAV infection.

Objective We established a rapid detection method of Pasteurella spp.and provided a reference for microbiological quality control of laboratory animal .Methods According to the β subunit of bacterial RNA polymerase ( rpoB) protein multiple alignments of 13 different Pasteurella spp.published in NCBI .The degenerate primers were designed by CODEHOP designer online .CODEHOP PCR method was applied to detecting Pasteurella spp.after the specificity and sensitivity of the method had been evaluated by 21 reference strains .Results Standard strain amplified fragment were about 200 bp by degenerate primers PastF6/PastR5.The primers are able to distinguish between Pasteurella spp.and the other pathognic organisms of laboratory animal respiratory tracts .Sensitivity of this method were 0.2 pg/μL~2 pg/μL to different Pasteurella.The Pasteurella positive rate was 19.1% in 609 animal ' s respiratory samples .The accuracy of positive results was 100%through verifying by sequenced and blast .Conclusions The established method has good specificity and sensitivity .It can be used to detect Pasteurella spp.in animal samples .

Objective To establish a tree shrew model of Fusarium solani keractitis by injecting Fusarium solani conidia into the corneal stroma.Methods Fusarium solani was inoculated into Sabouraud culture medium and incubated at 26℃ for 7 days.Fungal suspension was collected and the number of spores was adjusted to 1 × 1010 CFU /mL on the blood cell count plate.Forty healthy tree shrews were randomly divided into experimental group (n=30) and control group (n=10).In the experimental group, 50 μL of fungal spore suspension was injected into the cornea center with a 29G needle, and 50 μL saline was injected in the control group.The models were evaluated by anterior segment photography, in vivo confocal microscopy, histopathology, and corneal tissue culture.Results The fungal infiltration, the degree of edema of corneal epithelial and endothelial cells, and the number of mycelium were positively correlated with time.The number of infiltrating inflammatory cells, mainly, neutrophils, reached a peak on the 7th day after modeling.The mycelial growth was parallel to the stromal fibers.After the successful establishment of the model, the corneal tissue culture showed the growth of Fusarium solani.The successful rate of modeling was 86%.Conclusions The tree shrew model of Fusarium solani keratitis is established by injecting spores of Fusarium solani into the cornea.

Objective To isolate and identify viruses from fecal samples of tree shrew with diarrhea.Methods Fecal sample supernatant of tree shrew with diarrhea was inoculated to three cell lines ( Vero, LLC-MK2 and KMB17 ) , and the cytopathic effects on the cells were observed.The infectious particles in the culture supernatant were further ana-lyzed by transmission electron microscopy ( TEM) , genomic RNA-PAGE, rotavirus detection kit, amplification of S1 com-plete segment and bioinformatics analysis.Results Constant cytopathic effects were induced in Vero, LLC-MK2 and KMB17 cell lines after three passages of culture.The results from TEM, RNA-PAGE and rotavirus analysis indicated that they belong to reoviruses.Analysis of the S1 segments revealed that the S1 sequence from KMB17 cell culture had the high-est homology with that of prototype isolate T1L (85%nucleotide homology and 90%amino acid homology), therefore this isolate was named as type I reovirus.The other two S1 sequences from LLC-MK2 and Vero cell culture were identical to have 85%nucleotide homology and 92%amino acid homology with the prototype isolate T3D, named as type III reovirus. Phylogenetic analysis indicated that the isolates in this study are evolutionally adapted to tree shrews.Conclusions It is the first report here that 2 genotypes of Tupaia orthoreovirus are isolated and identified from one fecal sample via three cell lines and viral S1-specific primers, which provides useful guidelines for the isolation and identification of other reoviruses from tree shrew or other hosts.

Tree shrews get more and more concerns due to many of its physiological , biochemical and anatomical characteristics similar to those of human beings .Therefore, tree shrews models of human diseases such as viral diseases , neurological diseases and tumors attract more and more attention of researchers .In this article we will review the recent ad-vances in application of tree shrew models in research on human viral diseases .