Objective To explore the diagnostic value of CT imaging of creeping fat(CF)combined with serum optimization indicators for active Crohn's disease(CD).Methods The data from 68 cases underwent small intestine CT examinations(43 cases in the active CD group and 25 cases in the control group)were collected.CF imaging(non-contrast CT value,enhanced ΔCT value,submucosal fat deposition,and extent of intestinal wrapping)and serum optimization indicators[including neutrophil-to-lymphocyte ratio(NLR),C-reactive protein-to-albumin ratio(CAR),platelet-to-lymphocyte ratio(PLR),platelet-to-albumin ratio(PAR),neutrophil percentage-to-albumin ratio(NPAR),systemic immune-inflammation index(SII),lymphocyte-to-monocyte ratio(LMR),and albumin-to-globulin ratio(AGR)]were evaluated.The correlation between these idictors and CD activity were evaluated.Logistic regression and receiver operating characteristic(ROC)curve analysis were used to assess the diagnostic value of individual and combined indicators for active CD.Results Significant differences were observed in CF imaging and serum optimization indicators between the mild to moderate active CD groups and the control group(P<0.05).The area under the curve(AUC)for non-contrast CT value,arterial phase ΔCT value,venous phase ΔCT value,CF score,NLR,CAR,PLR,PAR,NPAR,SII and combined indicators in predicting moderate active CD were 0.914,0.856,0.923,0.703,0.902,0.880,0.858,0.717,0.802,0.875 and 0.972,respectively.Among these,venous phase ΔCT value and SII exhibited the highest sensitivity(100%),while non-contrast CT value and NPAR demonstrated the highest specificity(87.5%).Venous phase ΔCT value and CAR were identified as significant predictors of moderate active CD(P<0.05).Additionally,serum optimization indicators were significantly correlated with CF imaging(P<0.01).Conclusion CF imaging and serum optimization indicators serve as potential non-invasive markers for diagnosing active CD,with higher diagnostic value achieved through combined assessments.Venous phase ΔCT value and CAR are promising predictors for moderate active CD.

Objective To explore the diagnostic value of CT imaging of creeping fat(CF)combined with serum optimization indicators for active Crohn's disease(CD).Methods The data from 68 cases underwent small intestine CT examinations(43 cases in the active CD group and 25 cases in the control group)were collected.CF imaging(non-contrast CT value,enhanced ΔCT value,submucosal fat deposition,and extent of intestinal wrapping)and serum optimization indicators[including neutrophil-to-lymphocyte ratio(NLR),C-reactive protein-to-albumin ratio(CAR),platelet-to-lymphocyte ratio(PLR),platelet-to-albumin ratio(PAR),neutrophil percentage-to-albumin ratio(NPAR),systemic immune-inflammation index(SII),lymphocyte-to-monocyte ratio(LMR),and albumin-to-globulin ratio(AGR)]were evaluated.The correlation between these idictors and CD activity were evaluated.Logistic regression and receiver operating characteristic(ROC)curve analysis were used to assess the diagnostic value of individual and combined indicators for active CD.Results Significant differences were observed in CF imaging and serum optimization indicators between the mild to moderate active CD groups and the control group(P<0.05).The area under the curve(AUC)for non-contrast CT value,arterial phase ΔCT value,venous phase ΔCT value,CF score,NLR,CAR,PLR,PAR,NPAR,SII and combined indicators in predicting moderate active CD were 0.914,0.856,0.923,0.703,0.902,0.880,0.858,0.717,0.802,0.875 and 0.972,respectively.Among these,venous phase ΔCT value and SII exhibited the highest sensitivity(100%),while non-contrast CT value and NPAR demonstrated the highest specificity(87.5%).Venous phase ΔCT value and CAR were identified as significant predictors of moderate active CD(P<0.05).Additionally,serum optimization indicators were significantly correlated with CF imaging(P<0.01).Conclusion CF imaging and serum optimization indicators serve as potential non-invasive markers for diagnosing active CD,with higher diagnostic value achieved through combined assessments.Venous phase ΔCT value and CAR are promising predictors for moderate active CD.

Objective To establish an immortalized tree shrew corneal stromal cells(CSCs)line and to study its response to virus infection.Methods Primary tree shrew CSCs were isolated and cultured by the tissue block adhesion method.CSCs were then transfected with a lentivirus carrying the SV40T gene and monoclonal cells were selected for passage culture.The characteristics of the CSCs were investigated by morphological observation and compared with 40 generations until the 50 generations or more,immunofluorescence identification of vimentin and SV40T genes,karyotype examination,and cell proliferation curve.The CSCs were infected with herpes simplex virus-1(HSV-1)(McKrae strain),Zika virus(ZIKV,GZ01 strain),Dengue virus typeⅡ,and H1N1(PR8).Results The immortalized tree shrew CSCs after＞50 passages appeared spindle-shaped with good cell morphology and structure compared with 40 generations.Positive immunofluorescence expression of vimentin and SV40T genes.The cell growth curve showed that the cells were in logarithmic-phase growth on days 4～5 and grew vigorously.The number of chromosomes in the primary cells was stable at 62,while immortalized CSCs had 64 chromosomes at P21 and P56.The virus titer results showed that the immortalized tree shrew CSCs were sensitive to HSV-1(McKrae strain),ZIKV(GZ01 strain),Dengue virus typeⅡ,and H1N1(PR8),with virus titers of 1.32×105,5.62×106,2.69×107,and 7.76×104 CCID50/mL,respectively.Conclusions The immortalized tree shrew CSCs were established successfully,suggesting that this cell line is suitable for studies of the mechanisms of HSV,ZIKV,Dengue virus,and influenza A virus infection in relation to corneal diseases and antiviral drugs.

Objective:To explore the expression level and clinical significance of low-density granulocytes (LDGs) in peripheral blood of patients with inflammatory bowel disease (IBD) .Methods:A cross-sectional survey was conducted. Clinical data of IBD patients admitted to the Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University from August 2022 to January 2023 were collected. A total of 45 healthy people were enrolled as the normal control group. Simple endoscopic score for Crohn′s disease (SES-CD) was used to evaluate the activity of Crohn′s disease (CD) and Mayo endoscopic score (MES) was used to evaluate the activity of ulcerative colitis (UC). Laboratory indicators included LDGs, white blood cell count (WBC), hemoglobin (HB), platelet count (PLT), C-reactive protein (CRP), neutrophil to lymphocyte ratio (NLR), and fecal calprotectin (FC). The difference in LDGs level between IBD patients and the normal control group was statistically analyzed and the relationship between LDGs level and clinical characteristics of IBD patients was analyzed. Receiver operating characteristic (ROC) curve was used to calculate the predictive value of LDGs for the disease activity of IBD.Results:A total of 130 IBD patients were included, including 83 CD patients and 47 UC. Compared with the normal control group, the LDGs levels of patients in the CD group and UC group were respectively higher[CD: 0.53% (0.32%, 1.41%) vs. 0.19% (0.12%, 0.29%), H= 57.71, P<0.001; UC: 0.87% (0.43%, 1.90%) vs. 0.19% (0.12%, 0.29%), H= 73.23, P<0.001]. Compared with the CD patients in remission phase, the level of LDGs in active CD patients was higher ( P<0.001). Compared with the CD patients in mild activity phase, the LDGs levels of CD patients in moderate and severe activity phases were higher (all P<0.05). In terms of different disease behaviours, the level of LDGs in stricturing CD patients was the highest [1.37% (0.91%, 3.06%), all P<0.05]. Compared with the UC patients in remission phase, the level of LDGs in active UC patients was higher ( P<0.001). Compared with the UC patients in mild activity phase, the LDGs levels of UC patients in moderate and severe activity phases were higher (all P<0.05). In the CD group, LDGs level was positively correlated with CRP, FC, NLR, PLT, simple Crohn′s disease activity index (CDAI), and SES-CD ( r= 0.374, 0.548, 0.345, 0.284, 0.764, 0.721, all P<0.05). In the UC group, LDG levels was positively correlated with CRP, FC, NLR, Sutherland disease activity index (DAI), and MES ( r= 0.325, 0.666, 0.474, 0.638, 0.740, all P<0.05). In CD patients, the cut-off value of LDGs was 0.565%, with the area under curve (AUC) of 0.873, sensitivity of 73.50%, and specificity of 99.30%. In UC patients, the cut-off value of LDGs was 0.545%, with the AUC of 0.877, sensitivity of 76.90%, and specificity of 100.00%. Conclusion:The expression of LDGs in IBD patients is significantly high, which correlates with the disease activity and may be used as a biological marker for the clinical evaluation of IBD patients.

Objective:To explore the expression level and clinical significance of low-density granulocytes (LDGs) in peripheral blood of patients with inflammatory bowel disease (IBD) .Methods:A cross-sectional survey was conducted. Clinical data of IBD patients admitted to the Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University from August 2022 to January 2023 were collected. A total of 45 healthy people were enrolled as the normal control group. Simple endoscopic score for Crohn′s disease (SES-CD) was used to evaluate the activity of Crohn′s disease (CD) and Mayo endoscopic score (MES) was used to evaluate the activity of ulcerative colitis (UC). Laboratory indicators included LDGs, white blood cell count (WBC), hemoglobin (HB), platelet count (PLT), C-reactive protein (CRP), neutrophil to lymphocyte ratio (NLR), and fecal calprotectin (FC). The difference in LDGs level between IBD patients and the normal control group was statistically analyzed and the relationship between LDGs level and clinical characteristics of IBD patients was analyzed. Receiver operating characteristic (ROC) curve was used to calculate the predictive value of LDGs for the disease activity of IBD.Results:A total of 130 IBD patients were included, including 83 CD patients and 47 UC. Compared with the normal control group, the LDGs levels of patients in the CD group and UC group were respectively higher[CD: 0.53% (0.32%, 1.41%) vs. 0.19% (0.12%, 0.29%), H= 57.71, P<0.001; UC: 0.87% (0.43%, 1.90%) vs. 0.19% (0.12%, 0.29%), H= 73.23, P<0.001]. Compared with the CD patients in remission phase, the level of LDGs in active CD patients was higher ( P<0.001). Compared with the CD patients in mild activity phase, the LDGs levels of CD patients in moderate and severe activity phases were higher (all P<0.05). In terms of different disease behaviours, the level of LDGs in stricturing CD patients was the highest [1.37% (0.91%, 3.06%), all P<0.05]. Compared with the UC patients in remission phase, the level of LDGs in active UC patients was higher ( P<0.001). Compared with the UC patients in mild activity phase, the LDGs levels of UC patients in moderate and severe activity phases were higher (all P<0.05). In the CD group, LDGs level was positively correlated with CRP, FC, NLR, PLT, simple Crohn′s disease activity index (CDAI), and SES-CD ( r= 0.374, 0.548, 0.345, 0.284, 0.764, 0.721, all P<0.05). In the UC group, LDG levels was positively correlated with CRP, FC, NLR, Sutherland disease activity index (DAI), and MES ( r= 0.325, 0.666, 0.474, 0.638, 0.740, all P<0.05). In CD patients, the cut-off value of LDGs was 0.565%, with the area under curve (AUC) of 0.873, sensitivity of 73.50%, and specificity of 99.30%. In UC patients, the cut-off value of LDGs was 0.545%, with the AUC of 0.877, sensitivity of 76.90%, and specificity of 100.00%. Conclusion:The expression of LDGs in IBD patients is significantly high, which correlates with the disease activity and may be used as a biological marker for the clinical evaluation of IBD patients.

Influenza is a highly contagious disease that mainly affects the respiratory system and often leads to lung complications. Also it can cause a variety of very rare and serious neurological complications, including Guillain-Barre syndrome, transverse myelitis, meningoencephalitis and others. In recent years, neurological complications caused by influenza A virus have been reported in many countries and regions, and gradually attracted international attention. However, the pathogenesis of this complication remains unclear, and there are few related cases and animal experimental studies,and no specific treatment. Therefore, the authors summarized the study of neurological complications caused by influenza A virus in human and laboratory animals, in order to have a comprehensive understanding of the neurological diseases caused by influenza A virus.

OBJECTIVE: To detect the levels of Epstein-Barr virus (EBV) latent membrane protein 1 Antibodies (LMP1-Ab) in nasopharyngeal carcinoma (NPC)sera and discuss the clinical significance of this test in diagnosis, prognosis, and immune-targeted therapy of NPC. METHOD: Enzyme-linked immunosorbent assay (ELISA) and Western blot method were used to detect the LMP1-Ab levels in 61 NPC sera, 30 nasopharyngitis sera, and 55 normal sera. The relationship between the LMP1-Ab level and clinical and pathological features of NPC was analyzed. RESULT: ELISA test showed that LMP1 antibodies level was significantly higher in nasopharyngeal carcinoma group than those in nasopharyngitis group and in healthy group and there were statistical significances (all P<0.05). In SNPC group, the LMP1-Ab level was not related to the pathological grade and cervical lymph node metastases (P>0.05). Western blot test revealed that the expression of LMP1 antibodies was higher in NPC sera than in nasopharyngitis sera and in normal sera. CONCLUSION LMP1-Ab level was higher in nasopharyngeal carcinoma group than in nasopharyngitis group and in normal group. Therefore, LMP1 may be considered as a tumor correlated antigen to help the diagnosis and immune-targeted therapy of NPC.
Adolescent ; Adult ; Aged ; Antibodies, Viral ; blood ; Carcinoma ; Case-Control Studies ; Enzyme-Linked Immunosorbent Assay ; Female ; Herpesvirus 4, Human ; immunology ; Humans ; Male ; Middle Aged ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; blood ; pathology ; Viral Matrix Proteins ; immunology ; Young Adult