Objective:To analyze the expression of pentraxin-3 (PTX-3) and cyclooxygenase-2 (COX-2) in patients with polycystic ovary syndrome (PCOS) and their relationship with insulin resistance.Methods::A total of 150 PCOS patients diagnosed and treated in our hospital from Dec. 2020 to Mar. 2023 were collected as the study subjects. According to the insulin resistance index (HOMA-IR), 68 cases were in the insulin resistance group and 82 cases in the non insulin resistance group. 150 healthy individuals who underwent physical examination were included into the control group; Enzyme linked immunosorbent assay (ELISA) was applied to detect serum levels of PTX-3 and COX-2; Pearson method was applied to analyze the correlation between serum PTX-3 and COX-2 levels, and their correlation with general data; Logistic regression was applied to analyze the influencing factors of insulin resistance in PCOS patients; Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of serum PTX-3 and COX-2 for insulin resistance in PCOS patients.Results::Fasting blood sugar (FBG) [ (5.46±1.12), (4.71±1.03) mmol/L] in insulin-resistant and non-insulin-resistant groups, fasting insulin (FINS), HOMA-IR, testosterone (T), estradiol (E2), total cholesterol (TC), triglycerides (TG), LDL cholesterol (LDL-C), PTX-3, and COX-2 were significantly higher than those in the control group ( P<0.05), HDL cholesterol (HDL-C) were significantly lower than those in the control group ( P<0.05), and the insulin-resistant group FBG, FINS, HOMA-IR, T, E2, TC, TG, LDL-C, PTX-3 and COX-2 levels were significantly higher than those in the non-insulin-resistant group ( P<0.05), and HDL-C levels were significantly lower than those in the non-insulin-resistant group ( P<0.05). According to Pearson correlation analysis, serum PTX-3 and COX-2 levels were positively correlated ( r=0.587, P<0.05), both of which were positively correlated with FBG, FINS, HOMA-IR, E2, T, TC, TG and LDL-C, and negatively correlated with HDL-C. According to Logistic regression analysis, the elevated levels of PTX-3, COX-2, FBG, FINS, and HOMA-IR were risk factors affecting insulin resistance in PCOS patients ( P<0.05). According to the ROC curve, the AUC of insulin resistance in PCOS patients diagnosed by the combination of the two was better than that diagnosed separately by PTX-3 and COX-2 (Z combination vs PTX-3=2.205, Z combination vs COX-2=2.703, P<0.05) . Conclusions::The expression levels of PTX-3 and COX-2 in the serum of PCOS patients are obviously increased, and they are closely related to insulin resistance. The combination of the two can improve the diagnostic value of insulin resistance in PCOS patients.

Objective:To analyze the expression of pentraxin-3 (PTX-3) and cyclooxygenase-2 (COX-2) in patients with polycystic ovary syndrome (PCOS) and their relationship with insulin resistance.Methods::A total of 150 PCOS patients diagnosed and treated in our hospital from Dec. 2020 to Mar. 2023 were collected as the study subjects. According to the insulin resistance index (HOMA-IR), 68 cases were in the insulin resistance group and 82 cases in the non insulin resistance group. 150 healthy individuals who underwent physical examination were included into the control group; Enzyme linked immunosorbent assay (ELISA) was applied to detect serum levels of PTX-3 and COX-2; Pearson method was applied to analyze the correlation between serum PTX-3 and COX-2 levels, and their correlation with general data; Logistic regression was applied to analyze the influencing factors of insulin resistance in PCOS patients; Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of serum PTX-3 and COX-2 for insulin resistance in PCOS patients.Results::Fasting blood sugar (FBG) [ (5.46±1.12), (4.71±1.03) mmol/L] in insulin-resistant and non-insulin-resistant groups, fasting insulin (FINS), HOMA-IR, testosterone (T), estradiol (E2), total cholesterol (TC), triglycerides (TG), LDL cholesterol (LDL-C), PTX-3, and COX-2 were significantly higher than those in the control group ( P<0.05), HDL cholesterol (HDL-C) were significantly lower than those in the control group ( P<0.05), and the insulin-resistant group FBG, FINS, HOMA-IR, T, E2, TC, TG, LDL-C, PTX-3 and COX-2 levels were significantly higher than those in the non-insulin-resistant group ( P<0.05), and HDL-C levels were significantly lower than those in the non-insulin-resistant group ( P<0.05). According to Pearson correlation analysis, serum PTX-3 and COX-2 levels were positively correlated ( r=0.587, P<0.05), both of which were positively correlated with FBG, FINS, HOMA-IR, E2, T, TC, TG and LDL-C, and negatively correlated with HDL-C. According to Logistic regression analysis, the elevated levels of PTX-3, COX-2, FBG, FINS, and HOMA-IR were risk factors affecting insulin resistance in PCOS patients ( P<0.05). According to the ROC curve, the AUC of insulin resistance in PCOS patients diagnosed by the combination of the two was better than that diagnosed separately by PTX-3 and COX-2 (Z combination vs PTX-3=2.205, Z combination vs COX-2=2.703, P<0.05) . Conclusions::The expression levels of PTX-3 and COX-2 in the serum of PCOS patients are obviously increased, and they are closely related to insulin resistance. The combination of the two can improve the diagnostic value of insulin resistance in PCOS patients.