Objective:To investigate the effects of infection on circulating tumor cells (CTC) and tumor markers levels in patients after radical gastrectomy.Methods:A retrospective case series study was conducted. A total of 100 patients who underwent radical gastrectomy admitted to Chang'an Hospital in Xi'an from January 2020 to December 2023 were selected. The patients were divided into the infection group and the non-infection group according to whether the patients were infected or not after surgery. The general clinical data, CTC positive rate on the third day after surgery and peripheral blood tumor markers levels between the 2 groups were compared.Results:Among 100 gastric cancer patients who underwent radical gastrectomy, 21 cases (21%) developed postoperative infection, mainly including respiratory infection (38.10%, 8/21) and surgical site infection (23.8%, 5/21). The infection group experienced longer surgery durations [(3.4±1.0) h vs. (3.0±0.4) h] and more intraoperative blood loss [(415±93) ml vs. (370±75) ml] compared to the non-infection group, and the differences were statistically significant (all P < 0.05). The positive rate of CTC, white blood cells, neutrophil-to-lymphocyte ratio, carbohydrate antigen 153 (CA153), carbohydrate antigen 199 (CA199), and carcinoembryonic antigen (CEA) levels in the infection group were higher than those in the non-infection group on the third day after surgery, and the differences were significantly significant (all P < 0.05). Levels of gastrin and motilin in the infection group were lower than those in the non-infection group on the third day after surgery, and the differences were statistically significant (all P < 0.05). Conclusions:Postoperative infection of gastric cancer causes an increase in CTC positive rate, peripheral blood CA135, CA199, CEA levels in patients after radical gastrectomy, which affects the patients' recovery of gastrointestinal function.

Approximately 140 million people worldwide are homozygous carriers of APOE4 (ε4), a strong genetic risk factor for late onset familial and sporadic Alzheimer's disease (AD), 91% of whom will develop AD at earlier age than heterozygous carriers and noncarriers. Susceptibility to AD could be reduced by targeted editing of APOE4, but a technical basis for controlling the off-target effects of base editors is necessary to develop low-risk personalized gene therapies. Here, we first screened eight cytosine base editor variants at four injection stages (from 1- to 8-cell stage), and found that FNLS-YE1 variant in 8-cell embryos achieved the comparable base conversion rate (up to 100%) with the lowest bystander effects. In particular, 80% of AD-susceptible ε4 allele copies were converted to the AD-neutral ε3 allele in human ε4-carrying embryos. Stringent control measures combined with targeted deep sequencing, whole genome sequencing, and RNA sequencing showed no DNA or RNA off-target events in FNLS-YE1-treated human embryos or their derived stem cells. Furthermore, base editing with FNLS-YE1 showed no effects on embryo development to the blastocyst stage. Finally, we also demonstrated FNLS-YE1 could introduce known protective variants in human embryos to potentially reduce human susceptivity to systemic lupus erythematosus and familial hypercholesterolemia. Our study therefore suggests that base editing with FNLS-YE1 can efficiently and safely introduce known preventive variants in 8-cell human embryos, a potential approach for reducing human susceptibility to AD or other genetic diseases.
Humans ; Apolipoprotein E4/genetics* ; Cytosine ; Mutation ; Blastocyst ; Heterozygote ; Gene Editing ; CRISPR-Cas Systems

Objective To investigate the surgical treatment strategy of intracranial alveolar echinococcosis(AE)and the clinical outcomes.Methods The clinical and follow-up data of 15 intracranial AE patients who underwent surgical treatment in the Departments of Neurosurgery of Sichuan Provincial People's Hospital(SPPH)and People's Hospital of Aba Tibetan and Qiang Autonomous Prefecture(a branch hospital of SPPH)between March 2017 and January 2021 were retrospectively analyzed.Full follow-up data were available for each of the 15 cases.The clinical and imaging characteristics,general surgical information,and surgical outcomes were analyzed.Results In the 15 patients,there were a total of 50 intracranial lesions,with an average of(3.3±3.1)/case.Four cases had solitary intracranial lesions,while 11 cases had multiple lesions,with the number of intracranial lesions per case ranging from 2 to 13.All patients with solitary intracranial lesions received total resection.In 6 patients with multiple intracranial lesions,only the largest lesion was surgically removed,and in 5 patients,2 to 3 adjacent lesions were surgically removed.All but one patient had extracranial lesions in their liver,lungs,kidneys,adrenal glands,and thoracic vertebrae.The patients were followed up for 12 to 58 months after surgery,with the mean follow-up time being(28.1±13.4)months.Among the 15 cases,13 showed stable intracranial condition during postoperative follow-up.Intracranial lesions recurred in 2 patients who had deep lesions accompanied by dissemination to the subarachnoid space.Two patients died during follow-up.Conclusion Microsurgical treatment of intracranial AE is effective,but total surgical resection is difficult to accomplish when patients have echinococcosis lesions located at a depth,especially when the lesions are spreading to the subarachnoid space.The prognosis of patients is closely associated with the extent of lesion invasion and the control of systemic hydatid lesions,especially those in the liver.

Excessive theta (θ) frequency oscillation and synchronization in the basal ganglia (BG) has been reported in elderly parkinsonian patients and animal models of levodopa (L-dopa)-induced dyskinesia (LID), particularly the θ oscillation recorded during periods when L-dopa is withdrawn (the off L-dopa state). To gain insight into processes underlying this activity, we explored the relationship between primary motor cortex (M1) oscillatory activity and BG output in LID. We recorded local field potentials in the substantia nigra pars reticulata (SNr) and M1 of awake, inattentive resting rats before and after L-dopa priming in Sham control, Parkinson disease model, and LID model groups. We found that chronic L-dopa increased θ synchronization and information flow between the SNr and M1 in off L-dopa state LID rats, with a SNr-to-M1 flow directionality. Compared with the on state, θ oscillational activity (θ synchronization and information flow) during the off state were more closely associated with abnormal involuntary movements. Our findings indicate that θ oscillation in M1 may be consequent to abnormal synchronous discharges in the BG and support the notion that M1 θ oscillation may participate in the induction of dyskinesia.

Frogs were caught from 4 towns in Huaxi of Guiyang and dissected.The collected spargana were used to infect young dogs for species identification.Results showed that the wild frogs were identified as Rana nigromaculata, and the infection rate was 16%(131/818) with an average intensity infection of 3.44 per frog, The tapeworm obtained from an infected dog was specified as Spirometra mansoni.