Objective To mine and analyze the routine blood test data of children with allergic rhinitis (AR), identify routine blood parameters related to childhood allergic rhinitis, establish an effective diagnostic model, and evaluate the performance of the model. Methods This study was a retrospective study of clinical cases. The experimental group comprised a total of 1110 children diagnosed with AR at the First Affiliated Hospital of Air Force Medical University during the period from December 12, 2020 to December 12, 2021, while the control group included 1109 children without a history of allergic rhinitis or other allergic diseases who underwent routine physical examinations during the same period. Information such as age, sex and routine blood test results was collected for all subjects. The levels of routine blood test indicators were compared between AR children and healthy children using comprehensive intelligent baseline analysis, with indicators of P≥0.05 excluded; variables were screened by Lasso regression. Binary Logistic regression was used to further evaluate the influence of multiple routine blood indexes on the results. Five kinds of machine model algorithms were used, namely extreme value gradient lift (XGBoost), logistic regression (LR), gradient lift decision tree (LGBMC), Random forest (RF) and adaptive lift algorithm (AdaBoost), to establish the diagnostic models. The receiver operating characteristic (ROC) curve was used to screen the optimal model. The best LightGBM algorithm was used to build an online patient risk assessment tool for clinical application. Results Statistically significant differences were observed between the AR group and the control group in the following routine blood test indicators: mean cellular hemoglobin concentration (MCHC), hemoglobin (HGB), absolute value of basophils (BASO), absolute value of eosinophils (EOS), large platelet ratio (P-LCR), mean platelet volume (MPV), platelet distribution width (PDW), platelet count (PLT), absolute values of leukocyte neutrophil (W-LCC), leukocyte monocyte (W-MCC), leukocyte lymphocyte (W-SCC), and age. Lasso regression identified these variables as important predictors, and binary Logistic regression further analyzed the significant influence of these variables on the results. The optimal machine learning algorithm LightGBM was used to establish a multi-index joint detection model. The model showed robust prediction performance in the training set, with AUC values of 0.8512 and 0.8103 in the internal validation set. Conclusion The identified routine blood parameters can be used as potential biomarkers for early diagnosis and risk assessment of AR, which can improve the accuracy and efficiency of diagnosis. The established model provides scientific basis for more accurate diagnostic tools and personalized prevention strategies. Future studies should prospectively validate these findings and explore their applicability in other related diseases.
Humans ; Male ; Female ; Rhinitis, Allergic/blood* ; Child ; Biomarkers/blood* ; Retrospective Studies ; Early Diagnosis ; Child, Preschool ; ROC Curve ; Logistic Models ; Hematologic Tests ; Algorithms ; Adolescent ; Machine Learning

Objective:To observe the clinical efficacy of against-lateral correction Tuina(Chinese therapeutic massage)in treating atlanto-axial joint disorder(AAJD)and imaging changes.Methods:A total of 142 patients with AAJD were recruited.They were randomly allocated to a trial group and a control group using the random number table method,with 71 participants in each group.The trial group was treated with against-lateral correction Tuina 3 times weekly.The control group was offered conventional physical traction therapy once daily.The interventions lasted 2 weeks in both groups.The two groups of participants were observed before and after treatment for their changes in the global pain scale(GPS)score,visual analog scale(VAS)score for dizziness assessment,cervical range of motion(ROM)in rotation,and the extent of atlanto-dental displacement.Results:The GPS and VAS scores dropped after treatment in both groups(P<0.05)and were lower in the trial group than in the control group after treatment and at the follow-up(P<0.05).Participants in the trial group achieved a significant increase in the cervical ROM in rotation after treatment and at the follow-up compared to the pre-treatment value(P<0.05)and surpassed the control group(P<0.05);the control group only showed an increase in the left-side rotation(P<0.05).After the intervention,neither the intra-group nor the between-group comparison revealed significant differences in the extent of atlanto-dental displacement(P>0.05),though the trial group presented an improving tendency.Conclusion:Compared to physical traction,the against-lateral correction Tuina method works more significantly in improving pain,dizziness,and ROM in AAJD patients.

Executive function(EF) is an advanced cognitive function of the central nervous system, and is closely related to an individual's capacity for daily living and adaptation. Patients with attention deficit hyperactivity disorder (ADHD) typically exhibit significant executive dysfunction. While most existing studies on the executive function of individuals with ADHD are cross-sectional, and little is known about the longitudinal maturation process of related brain structures and functional connectivity patterns. The findings indicate that ADHD patients exhibit differential developmental trajectories in brain structural and functional connectivity compared with typically developing group.Furthermore, there is a lifespan association between abnormal brain network development and ADHD symptoms. This article aims to elucidate the characteristics of executive function deficits in ADHD patients across different developmental stages, examining their relationship with the nervous system’s development from a development perspective.

Objective To search for and verify the genetic factors that lead to familial thoracic aortic aneurysm and dissection.Methods Patients with a family history were screened from clinical cases of thoracic aortic aneurysm and dissection,and their relevant clinical data were collected.After extracting the whole-genome DNA from the proband's blood samples,gene panel testing was carried out,and first-generation sequencing was used to verify the blood samples of the proband's immediate and collateral relatives.Meanwhile,relevant cell experiments were designed.First,the ACTA2-Leu195 * point mutation plasmid was constructed,and then the mutant plasmid and wild-type ACTA 2 were transfected into cells through expression plasmids respectively.After 48 hours,RNA and total protein were extracted,and the expression levels were detected by qPCR and Western blotting,respectively.Results Through high-throughput sequencing,the mutation site of c.583delC in the ACTA2 gene was found,and it was also confirmed that the heterozygous mutation at this site in the family was closely related to familial thoracic aortic aneurysm and dissection.The results of cell experiments showed that the mRNA and protein expression levels in the group transfected with the ACTA2-Leu195 * mutant plasmid were significantly decreased compared with those of the wild type,suggesting that the mutant could not express proteins normally.Conclusion Through clinical family studies and cell-level experiments,a new mutation site in ACTA2 leading to familial thoracic aortic aneurysm and dissection has been discovered,which provides a new approach for the screening,detection and clinical treatment of familial thoracic aortic aneurysm and dissection.

Executive function(EF) is an advanced cognitive function of the central nervous system, and is closely related to an individual's capacity for daily living and adaptation. Patients with attention deficit hyperactivity disorder (ADHD) typically exhibit significant executive dysfunction. While most existing studies on the executive function of individuals with ADHD are cross-sectional, and little is known about the longitudinal maturation process of related brain structures and functional connectivity patterns. The findings indicate that ADHD patients exhibit differential developmental trajectories in brain structural and functional connectivity compared with typically developing group.Furthermore, there is a lifespan association between abnormal brain network development and ADHD symptoms. This article aims to elucidate the characteristics of executive function deficits in ADHD patients across different developmental stages, examining their relationship with the nervous system’s development from a development perspective.

Specialist nurses assume an important role in clinical practice, teaching and research, and even in the development of the nursing discipline. Their professionalism is critical to improving the quality of nursing, enhancing the practice experience, and promoting organizational progress. This article reviews the current status, influencing factors, and measurement tools of professionalism among specialist nurses, with the aim of informing subsequent studies and intervention programs by nursing administrators.

Objective To search for and verify the genetic factors that lead to familial thoracic aortic aneurysm and dissection.Methods Patients with a family history were screened from clinical cases of thoracic aortic aneurysm and dissection,and their relevant clinical data were collected.After extracting the whole-genome DNA from the proband's blood samples,gene panel testing was carried out,and first-generation sequencing was used to verify the blood samples of the proband's immediate and collateral relatives.Meanwhile,relevant cell experiments were designed.First,the ACTA2-Leu195 * point mutation plasmid was constructed,and then the mutant plasmid and wild-type ACTA 2 were transfected into cells through expression plasmids respectively.After 48 hours,RNA and total protein were extracted,and the expression levels were detected by qPCR and Western blotting,respectively.Results Through high-throughput sequencing,the mutation site of c.583delC in the ACTA2 gene was found,and it was also confirmed that the heterozygous mutation at this site in the family was closely related to familial thoracic aortic aneurysm and dissection.The results of cell experiments showed that the mRNA and protein expression levels in the group transfected with the ACTA2-Leu195 * mutant plasmid were significantly decreased compared with those of the wild type,suggesting that the mutant could not express proteins normally.Conclusion Through clinical family studies and cell-level experiments,a new mutation site in ACTA2 leading to familial thoracic aortic aneurysm and dissection has been discovered,which provides a new approach for the screening,detection and clinical treatment of familial thoracic aortic aneurysm and dissection.

Objective:To observe the clinical efficacy of against-lateral correction Tuina(Chinese therapeutic massage)in treating atlanto-axial joint disorder(AAJD)and imaging changes.Methods:A total of 142 patients with AAJD were recruited.They were randomly allocated to a trial group and a control group using the random number table method,with 71 participants in each group.The trial group was treated with against-lateral correction Tuina 3 times weekly.The control group was offered conventional physical traction therapy once daily.The interventions lasted 2 weeks in both groups.The two groups of participants were observed before and after treatment for their changes in the global pain scale(GPS)score,visual analog scale(VAS)score for dizziness assessment,cervical range of motion(ROM)in rotation,and the extent of atlanto-dental displacement.Results:The GPS and VAS scores dropped after treatment in both groups(P<0.05)and were lower in the trial group than in the control group after treatment and at the follow-up(P<0.05).Participants in the trial group achieved a significant increase in the cervical ROM in rotation after treatment and at the follow-up compared to the pre-treatment value(P<0.05)and surpassed the control group(P<0.05);the control group only showed an increase in the left-side rotation(P<0.05).After the intervention,neither the intra-group nor the between-group comparison revealed significant differences in the extent of atlanto-dental displacement(P>0.05),though the trial group presented an improving tendency.Conclusion:Compared to physical traction,the against-lateral correction Tuina method works more significantly in improving pain,dizziness,and ROM in AAJD patients.

Specialist nurses assume an important role in clinical practice, teaching and research, and even in the development of the nursing discipline. Their professionalism is critical to improving the quality of nursing, enhancing the practice experience, and promoting organizational progress. This article reviews the current status, influencing factors, and measurement tools of professionalism among specialist nurses, with the aim of informing subsequent studies and intervention programs by nursing administrators.

Private health insurance(PHI)is an important component of China's multi-tiered medical security system,meeting people's multi-level and diversified health needs through supplementing and connecting with the basic medical insurance(BMI).It aims to draw on the practical experience of typical countries to provide implications for developing PHI that complements BMI in China.Considering the different models of medical security systems in various countries and the diverse functional positioning of PHI,France,Germany,Australia,the United Kingdom,and the United States are selected as reference objects.After examining the macro institutional systems of PHI,coverage population,benefits packages,characteristics of coordination with health care and medicines,as well as supporting regulatory measures,the experiences are summarized.Based on China's context,it proposes to improve institutional system building,enrich product supply,expand the insured population,explore the coverage list formulation,and refine supporting regulatory policies.