ObjectiveTo explore the impact of comprehensive vocational rehabilitation on quality of life and return-to-work outcomes for individuals with mild work-related disability. MethodsA longitudinal observational study was conducted. A total of 343 work-injured employees receiving comprehensive vocational rehabilitation intervention in Guangdong Work Injury Rehabilitation Hospital from June, 2023 to December, 2024 were selected. The 36-item Short Form Health Survey (SF-36) was adopted to assess their health-related quality of life at admission and discharge. All subjects were followed up within one year after discharge to collect their return-to-work status. The differences in each dimension score of SF-36 among patients with different return-to-work outcomes (return to original post, transfer to new post and non-return to work) were compared, and the independent influencing factors of return-to-work outcomes were identified. ResultsComprehensive vocational rehabilitation intervention significantly improved the scores of physical functioning (PF), role-physical (RP) and role-emotional (RE) among work-injured employees (P < 0.05). At discharge, compared with patients who failed to return to work, patients returning to original post obtained significantly higher scores in PF, RP, bodily pain (BP), general health (GH), vitality (VT), social functioning (SF) and mental health (MH), patients transferred to new post achieved significantly higher scores in PF, BP, GH and SF (P < 0.05). Taking non-return-to-work status as the reference category, the multinomial logistic regression model showed the adverse factors for return to original post included low educational attainment (college diploma or below), on-site placement service, increased lost workdays and low employment intention (pre-contemplation and contemplation stages); the protective factor was positive evaluation of health transition (HT); for patients transferred to new posts, adverse factors were low employment intention (pre-contemplation stage) and increased lost workdays, while protective factors covered on-site placement service and positive HT evaluation. ConclusionComprehensive vocational rehabilitation can improve the quality of life for injured workers. Better quality of life is positively associated with return-to-work outcomes. HT, education level, job placement mode, lost workdays and employment willingness are independent factors impacting return-to-work outcomes.

ObjectiveTo evaluate the effectiveness of stone needle thermocompression and massage compared to flurbiprofen gel patch in relieving chronic musculoskeletal pain in the shoulder and back， and to explore the potential mediating mechanism through muscle elasticity. MethodsA total of 120 patients with chronic musculoskeletal pain in the shoulder and back were randomly assigned to either stone needle group or flurbiprofen group， with 60 patients in each. The stone needle group received stone needle thermocompression and massage for 30 minutes， three times per week； the flurbiprofen group received flurbiprofen gel patch twice daily. Both groups were treated for 2 weeks. Pain improvement， as the primary outcome， was assessed using the Global Pain Scale （GPS） at baseline， after 2 weeks of treatment， and again 2 weeks post-treatment. To explore potential mechanisms， a mediator analysis was conducted by measuring changes in superficial and deep muscle elasticity using musculoskeletal ultrasound at baseline and after the 2-week treatment period. ResultsThe stone needle group showed significantly greater pain relief than the flurbiprofen group 2 weeks post-treatment. After adjusting for confounders related to pain duration， the between-group mean difference was -8.8 ［95% CI （-18.2， -0.7）， P＜0.05］. Part of the therapeutic effect was mediated by changes in deep muscle elasticity， with a mediation effect size of -1.5 ［95% CI （-2.0， -0.9）， P = 0.024］， accounting for 17.9% of the total effect. ConclusionStone needle thermocompression and massage can effectively relieve chronic musculoskeletal pain in the shoulder and back， partly through a mediating effect of improved deep muscle elasticity.

Objective To investigate the effect of long noncoding RNAMCTP1-AS1 on the proliferation and invasion of cervical cancer cells and its related mechanisms.Methods The Cancer Genome Atlas database was used to analyze the expression of MCTP1-AS1 in cervical cancer tissues and normal cervical tissues,and the correlation between the expression level of MCTP1-AS1 and the pathological stage of cervical cancer patients was analyzed.The expression of MCTP1-AS1 in cervical cancer cell lines HCC1106,HCC94,SiHa,Hela,C33A and normal cervical epithelial cells H8 was detected.Hela cells were transfected with pcDNA-Ctrl plasmid(Ctrl group)and pcDNA-MCTP1-AS1 plasmid(MCTP1-AS1 group),respectively.the proliferation and invasion ability of Hela cells were detected,respectively.the expression of proliferation proteins CDK2 and Cyclin A and invasion proteins N-cadherin and ZEB1 in Hela cells were detected,the targeting relationship between MCTP1-AS1 and miR-10a-5p were verified.The expression of miR-10a-5p in Hela cells was detected.Results Compared with normal cervical tissue,the expression of MCTP1-AS1 in cervical cancer tissue was significantly decreased(P＜0.01).The expression level of MCTP1-AS1 was negatively correlated with the pathological stage of cervical cancer patients(P＜0.01).Compared with H8 cells,the expression of MCTP1-AS1 in cervical cancer cell lines HCC1106,HCC94,SiHa,Hela,and C33A were significantly decreased(P＜0.01).Compared to Ctrl group,overexpression of MCTP1-AS1 significantly reduced the levels of proliferative proteins CDK2 and Cyclin A,as well as invasive proteins N-cadherin and ZEB1 in Hela cells.MCTP1-AS1 directly binds to miR-10a-5p(P＜0.01).Compared to Ctrl group,MCTP1-AS1 group showed a significant decrease in miR-10a-5p expression in Hela cells(P＜0.01).Conclusion MCTP1-AS1 expression is downregulated in cervical cancer tissues and cells,and MCTP1-AS1 expression is negatively correlated with the pathological stage of cervical cancer patients.MCTP1-AS1 inhibits the proliferation and invasion of cervical cancer cells by targeting miR-10a-5p.

Alzheimer's disease(AD)is a progressive neurodegenerative disease with hidden onset.Recent researches have shown that apolipoprotein E4(APOE4)is a high-risk genetic factor of AD,and the clinical symptoms in APOE4 genotype AD patients are closely related to metabolic disorders.This paper reviewed the role of APOE4 in the pathogenesis of AD,and it is indicated that APOE4 contributes to the injury of the cerebral blood-brain barrier,is associated with the disorder of cerebral glucose and lipid metabolism,and leads to the damage of the scavenging ability of microglia.Moreover,this paper explored the approach of early intervention of AD with traditional Chinese medicine(TCM)based on the APOE4 pathogenesis.Based on the pathogenesis of AD being deficiency in origin and excess in superficiality,and under the guidance of the principle of prevention before illness in TCM,it is proposed that early TCM intervention for APOE4 genotype AD patients with metabolic disorders can be performed through improving the phlegm-dampness constitution,by focusing on insulin resistance-related indicators,and with traditional Chinese drug therapy based on syndrome differentiation alone or together with comprehensive management of exercises,diet control and drug interventions,thus to reduce or delay the onset of APOE4 genotype AD.

Perimenopausal generalized anxiety disorder belongs to the category of"depression syndrome"in traditional Chinese medicine.It is caused by disharmony of blood vessels,stagnation of qi,and disturbance of the mind and brain,leading to excessive or insufficient qi in the five zang organs.Based on different clinical manifestations,it can be divided into five types of depression.In clinical treatment,Professor Zhuang Lixing applies the mind-regulating acupuncture therapy,with the main acupoints being Sishenzhen,Shenting(GV24),Yintang(EX-HN3),Shenmen(HT7),and Sanyinjiao(SP6).Additional acupoints can be selected according to the patient's symptoms.During the manipulation,the"guiding qi and harmonizing essence"reinforcing and reducing technique is used to regulate emotions and guide qi to relieve depression.This method has shown excellent clinical efficacy and is suitable for clinical promotion.

Objective:To explore the chain mediating effect of coping style and illness perception between intolerance of uncertainty and fear of disease progression in patients with intracranial aneurysm.Methods:A total of 270 patients with intracranial aneurysms admitted to the neurosurgery department of 2 hospitals in Guangzhou from October 2023 to June 2024 were selected by convenience sampling method. The general information questionnaire, intolerance of uncertainty scale-12, the brief disease perception questionnaire, medical coping mode questionnaire(MCMQ) and fear of progression questionnaire-short form were used to investigate the subjects. Statistical description and Spearman correlation analysis were performed using IBM SPSS 25.0 software, and AMOS 23.0 software was used to test and correct the structural equation models.Results:The total score of fear of disease progression in patients with intracranial aneurysms was 27.50(20.00, 34.00). The total score of intolerance of uncertainty was 23.50(19.75, 29.00). The sores of confrontation, avoidance and submission in MCMQ were 18.69±5.44, 12.00(10.00, 15.00) and 7.00(5.00, 9.00), respectively.The total score of illness perception was 37.23±11.60. Fear of disease progression was positively correlated with intolerance of uncertainty, submission and illness perception ( r=0.614, 0.696, 0.680, all P<0.01). The direct effect of intolerance of uncertainty on fear of disease progression was significant in patients with intracranial aneurysms ( β=0.431, P<0.001). Intolerance of uncertainty indirectly affected fear of disease progression through submission ( β=0.181, P<0.001) and illness perception ( β=0.092, P<0.001). Submission and illness perception played chain-mediating effect in the relationship between intolerance of uncertainty and fear of disease progression ( β=0.103, P<0.001). Conclusion:The intolerance of uncertainty in patients with intracranial aneurysms can positively predict the fear of disease progression. Submission and illness perception played a chain mediating role between intolerance of uncertainty and fear of disease progression in patients with intracranial aneurysms.

Objective:To explore the chain mediating effect of coping style and illness perception between intolerance of uncertainty and fear of disease progression in patients with intracranial aneurysm.Methods:A total of 270 patients with intracranial aneurysms admitted to the neurosurgery department of 2 hospitals in Guangzhou from October 2023 to June 2024 were selected by convenience sampling method. The general information questionnaire, intolerance of uncertainty scale-12, the brief disease perception questionnaire, medical coping mode questionnaire(MCMQ) and fear of progression questionnaire-short form were used to investigate the subjects. Statistical description and Spearman correlation analysis were performed using IBM SPSS 25.0 software, and AMOS 23.0 software was used to test and correct the structural equation models.Results:The total score of fear of disease progression in patients with intracranial aneurysms was 27.50(20.00, 34.00). The total score of intolerance of uncertainty was 23.50(19.75, 29.00). The sores of confrontation, avoidance and submission in MCMQ were 18.69±5.44, 12.00(10.00, 15.00) and 7.00(5.00, 9.00), respectively.The total score of illness perception was 37.23±11.60. Fear of disease progression was positively correlated with intolerance of uncertainty, submission and illness perception ( r=0.614, 0.696, 0.680, all P<0.01). The direct effect of intolerance of uncertainty on fear of disease progression was significant in patients with intracranial aneurysms ( β=0.431, P<0.001). Intolerance of uncertainty indirectly affected fear of disease progression through submission ( β=0.181, P<0.001) and illness perception ( β=0.092, P<0.001). Submission and illness perception played chain-mediating effect in the relationship between intolerance of uncertainty and fear of disease progression ( β=0.103, P<0.001). Conclusion:The intolerance of uncertainty in patients with intracranial aneurysms can positively predict the fear of disease progression. Submission and illness perception played a chain mediating role between intolerance of uncertainty and fear of disease progression in patients with intracranial aneurysms.

Objective:To explore the current situation and influencing factors of home care needs of primary caregivers of stroke patients based on the existence-relatedness-growth (ERG) need theory, so as to provide reference for the development of effective intervention programs in the future.Methods:The supplemental sequential mixed methods design was adopted. From December 2023 to June 2024, convenience sampling was used to select the primary home caregivers ( n=210) of stroke patients as study subjects in community health service centers in Guangzhou. The survey was conducted using the General Information Questionnaire, Barthel Index, and Professional Care Needs Questionnaire for Family Caregivers of Stroke. Multiple linear regression was used to analyze the influencing factors of home care need scores. In July 2024, 11 primary home caregivers of stroke patients were selected for semi-structured interviews using purposive sampling method. NVivo14.0 software was used for data storage and processing, and Colaizzi 7-step method was used for extracting and summarizing interview data. Results:In the quantitative study, 210 questionnaires were distributed and 202 were effectively collected, with an effective response rate of 96.19%. The total score for home care needs of 202 primary caregivers was (117.31±13.64). Multiple linear regression analysis showed that patients' marital status, history of falls, activities of daily living, caregiver's educational level, average daily caregiving time, and number of caregivers were the influencing factors of home care need score ( P<0.05). In the qualitative study, three themes were distilled, namely the need for disease knowledge and rehabilitation skills, the need for emotional and psychological interventions, and the need for social resource utilization and economic support. Integrating the results of quantitative and qualitative studies revealed that primary caregivers of stroke patients were characterized by congruence, complementarity, and expansiveness in the three dimensions of ERG needs, respectively. Conclusions:The home care needs of primary caregivers of stroke patients present multidimensional and hierarchical characteristics and are influenced by multiple factors such as the functional status of the patients and the characteristics of the caregivers. The quality of community care services should be strengthened, community resource allocation should be optimized, and training programs based on the hierarchy of needs should be constructed to meet the home care needs of primary caregivers of stroke patients.

Objective To investigate the effect of long noncoding RNAMCTP1-AS1 on the proliferation and invasion of cervical cancer cells and its related mechanisms.Methods The Cancer Genome Atlas database was used to analyze the expression of MCTP1-AS1 in cervical cancer tissues and normal cervical tissues,and the correlation between the expression level of MCTP1-AS1 and the pathological stage of cervical cancer patients was analyzed.The expression of MCTP1-AS1 in cervical cancer cell lines HCC1106,HCC94,SiHa,Hela,C33A and normal cervical epithelial cells H8 was detected.Hela cells were transfected with pcDNA-Ctrl plasmid(Ctrl group)and pcDNA-MCTP1-AS1 plasmid(MCTP1-AS1 group),respectively.the proliferation and invasion ability of Hela cells were detected,respectively.the expression of proliferation proteins CDK2 and Cyclin A and invasion proteins N-cadherin and ZEB1 in Hela cells were detected,the targeting relationship between MCTP1-AS1 and miR-10a-5p were verified.The expression of miR-10a-5p in Hela cells was detected.Results Compared with normal cervical tissue,the expression of MCTP1-AS1 in cervical cancer tissue was significantly decreased(P＜0.01).The expression level of MCTP1-AS1 was negatively correlated with the pathological stage of cervical cancer patients(P＜0.01).Compared with H8 cells,the expression of MCTP1-AS1 in cervical cancer cell lines HCC1106,HCC94,SiHa,Hela,and C33A were significantly decreased(P＜0.01).Compared to Ctrl group,overexpression of MCTP1-AS1 significantly reduced the levels of proliferative proteins CDK2 and Cyclin A,as well as invasive proteins N-cadherin and ZEB1 in Hela cells.MCTP1-AS1 directly binds to miR-10a-5p(P＜0.01).Compared to Ctrl group,MCTP1-AS1 group showed a significant decrease in miR-10a-5p expression in Hela cells(P＜0.01).Conclusion MCTP1-AS1 expression is downregulated in cervical cancer tissues and cells,and MCTP1-AS1 expression is negatively correlated with the pathological stage of cervical cancer patients.MCTP1-AS1 inhibits the proliferation and invasion of cervical cancer cells by targeting miR-10a-5p.

Objective:To explore the current situation and influencing factors of home care needs of primary caregivers of stroke patients based on the existence-relatedness-growth (ERG) need theory, so as to provide reference for the development of effective intervention programs in the future.Methods:The supplemental sequential mixed methods design was adopted. From December 2023 to June 2024, convenience sampling was used to select the primary home caregivers ( n=210) of stroke patients as study subjects in community health service centers in Guangzhou. The survey was conducted using the General Information Questionnaire, Barthel Index, and Professional Care Needs Questionnaire for Family Caregivers of Stroke. Multiple linear regression was used to analyze the influencing factors of home care need scores. In July 2024, 11 primary home caregivers of stroke patients were selected for semi-structured interviews using purposive sampling method. NVivo14.0 software was used for data storage and processing, and Colaizzi 7-step method was used for extracting and summarizing interview data. Results:In the quantitative study, 210 questionnaires were distributed and 202 were effectively collected, with an effective response rate of 96.19%. The total score for home care needs of 202 primary caregivers was (117.31±13.64). Multiple linear regression analysis showed that patients' marital status, history of falls, activities of daily living, caregiver's educational level, average daily caregiving time, and number of caregivers were the influencing factors of home care need score ( P<0.05). In the qualitative study, three themes were distilled, namely the need for disease knowledge and rehabilitation skills, the need for emotional and psychological interventions, and the need for social resource utilization and economic support. Integrating the results of quantitative and qualitative studies revealed that primary caregivers of stroke patients were characterized by congruence, complementarity, and expansiveness in the three dimensions of ERG needs, respectively. Conclusions:The home care needs of primary caregivers of stroke patients present multidimensional and hierarchical characteristics and are influenced by multiple factors such as the functional status of the patients and the characteristics of the caregivers. The quality of community care services should be strengthened, community resource allocation should be optimized, and training programs based on the hierarchy of needs should be constructed to meet the home care needs of primary caregivers of stroke patients.