Objective:To investigate the relationship between the Notch signaling pathway and expression of vascular relevant factors in rabbit deep Ⅱ degree burn model.Methods:A total of 120 New Zealand white rabbits were randomly divided into a block group,a model group,and a control group,The block group was injected 2 mg/kg γ-secretase inhibitor (GSI) once a day at 1 d before the model establishment,and 1-14 d after the deep Ⅱ degree burns model establishment.The model group were injected physiological saline at the same time.The control group was only injected with the same amount of saline.The expressions of vascular endothelial growth factor (VEGF),vascular endothelial growth factor receptor 2 (VEGFR-2),matrix metalloprotein 2 (MMP-2) and matrix metalloprotein 9 (MMP-9) were detected by immunohistochemistry.Results:The expressions of VEGF and VEGFR-2 in the model group and the block group were significantly increased within 21 days after modeling,while decreased after 21 days;the expressions of MMP-2 and MMP-9 were decreased within 21 days after modeling,while increased after 21 days,with significant differences compared with the control group (P＜0.05).The expressions of VEGF and VEGFR-2 in the model group were higher than that in the block group,and the expressions of MMP-2 and MMP-9 were lower than those in the block group (P＜0.05).The expression of VEGFR-2 was positively correlated with VEGF,while MMP-2 and MMP-9 were negatively correlated with VEGF within 21 days after modeling.Conclusion:In the rabbit deep Ⅱ degree burn model,the Notch signaling pathway was blocked to attenuate the expressions of VEGF and VEGFR-2,and to up-regulate the expressions of MMP-2 and MMP-9.

In order to study the antitumor function of the housefly antibacterial peptide in a more comprehensive way, this article studied the antitumor function of housefly antimicrobial peptides on the tumor cell proliferation, invasion and migration. To induce antibacterial peptides, we use the needle to prick housefly then go through a process of low temperature centrifugal, solid phase extraction and freeze?drying to obtain antibacterial peptides crude extract, and then adjust antibacterial peptides to different concentrations to inhibit CCK8, cell migration and invasion experiment. The experiment show that housefly antimicrobial peptide can inhibit HepG2 cells proliferation, and the dose?response characteristics is closely effected by its concentration. Low concentration of antibacterial peptides (60μg/mL) can also effectively inhibit the migration and invasion of HepG2 cells. In conclusion, the crude extractings of antibacterial peptides from housefly larvae can inhibit the proliferation, invasion and migration of HepG2 cells.